Association analysis of selected markers with litter size in mice:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Berlin
Köster
2012
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| Ausgabe: | Aufl. 2012 |
| Schriftenreihe: | Schriftenreihe Agrarwissenschaft
29 |
| Schlagworte: | |
| Online-Zugang: | Inhaltstext Inhaltsverzeichnis |
| Beschreibung: | Zsfassung in dt. Sprache |
| Beschreibung: | 117 S. Ill. |
| ISBN: | 9783895747847 |
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Datensatz im Suchindex
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IMAGE 1
TABLE O F CONTENTS
SECTION CONTENTS PAGE
II TABLE O F CONTENTS 3
II LIST O F ACRONYMS AND ABBREVIATIONS 6
III ABSTRACT 9
IV ABSTRAKT 10
1 INTRODUCTION 11
2 LITERATURE REVIEW. 12
2.1. FERTILITY AND LITTER SIZE 12
2.1.1. GENETIC CHARACTERISTICS O F FERTILITY TRAITS IN MICE 12
2.2. MARKER TRAIT ASSOCIATION STUDIES 16
2.2.1. QTLS ASSOCIATED WITH FERTILITY AND LITTER SIZE IN PIGS 18
2.2.2. SUITABILITY O F MICE AS ANIMAL MODELS IN FERTILITY RESEARCH 24
2.2.3 MICE MODELS FOR DETECTING QTL FOR FERTILITY AND LITTER SIZE 2 4
2.2.3.1. WILD MICE 25
2.2.3.2. INBRED MICE STRAINS 25
2.2.3.3. INBRED STRAINS DERIVATIVES 26
2.2.3.3.1. CONGENICS 27
2.2.3.3.2. INTER SPECIFIC RECOMBINANT CONGENIC STRAINS 28
2.2.3.3.3. KNOCKOUT MICE 29
2.3. MARKER ASSISTED SELECTION 33
3. MATERIAL AND METHODS 36
3.1. ANIMALS 36
3.2. LIST O F EQUIPMENT 37
3.3. LIST O F CHEMICALS AND REAGENTS 38
3.4. METHOD 4 0
3.4.1. DNA-ISOLATION 40
3.4.2. MICROSATELLITES 4 0
3.4.3. POLYMERASE CHAIN REACTION 4 0
3.4.4. GEL CASTING 41
3.4.5. GEL ELECTROPHORESIS 4 2
3.4.6. SILVER NITRATE STAINING 43
3.5. STATISTICAL METHODS 43
3
HTTP://D-NB.INFO/1020636254
IMAGE 2
3.5.1. STATISTICAL PARAMETERS 44
3.5.1.1. SAMPLE SIZE 44
3.5.1.2. AVERAGE LITTER SIZE 44
3.5.1.3. GENOTYPE COUNT 45
3.5.1.4. GENOTYPE FREQUENCIES 45
3.5.1.5. ALLELE FREQUENCIES 45
3.5.1.6. AVERAGE LITTER SIZE PER GENOTYPE CLASS PER MARKER LOCUS 46
3.5.1.7. ADDITIVE GENETIC EFFECT 46
3.5.1.8. DOMINANCE EFFECT 46
3.2.1.9. DEGREE O F DOMINANCE 46
3.5.2. STATISTICAL TESTS 47
3.5.2.1. CHI-SQUARE STATISTICS 47
3.5.2.2. ANALYSIS O F VARIANCE 47
3.5.2.3. REGRESSION TESTS 47
3.5.2.4. LIKELIHOOD RATIO STATISTICS 48
3.5.2.5. PERMUTATION TESTS 48
3.5.2.6. STRATIFIED PERMUTATION 49
3.6. STATISTICAL SOFTWARE 49
3.6.1. OPEN OFFICE.ORG STATISTICS CALCULATOR. 49
3.6.2. MAP MANAGER QTXB20 49
4. RESULTS 51
4.1. PHENOTYPE DATA 51
4.1.1. LITTER SIZE HETEROSIS 51
4.1.2. LITTER SIZE AVERAGES, VARIANCES AND STANDARD DEVIATIONS 52
4.1.3. FAMILY EFFECT ON LITTER SIZE 52
4.2. GENOTYPE DATA 53
4.2.1. GENOTYPE COUNT 56
4.2.2. FAMILY EFFECT ON GENOTYPE COUNT AND AVERAGE LITTER SIZE 56
4.3. SELECTIVE GENOTYPING THE EXTREME PERFORMANCE GROUPS 57
4.3.1. AVERAGE LITTER SIZE PER GENOTYPE CLASS PER MARKER LOCUS 57
4.3.2. ADDITIVE EFFECT, DOMINANCE AND SUPERDOMINANCE 61
4.4. DNA-MARKER LOCUS AND TRAIT ASSOCIATION ANALYSIS 62
4.4.1. STATISTICAL RESULTS FOR MARKER TRAIT ASSOCIATION 62
4
IMAGE 3
4.4.1.1. CHI-SQUARE ANALYSIS 62
4.4.2. QTLS VERIFICATION USING KNOCKOUT MICE AS CONGENICS 65
4.5. HOMOLOGY: MUS MUSCULUS AND SUS SCROFA FOR LOCI AFFECTING LITTER
SIZE 66
5. DISCUSSION 69
5.1. PHENOTYPE DATA 69
5.1.1. SELECTIVE GENOTYPING 69
5.1.2. LITTER SIZE 70
5.1.3. MODE O F INHERITANCE FOR LITTER SIZE 70
5.2. GENOTYPIC DATA 72
5.2.1. GENOTYPE COUNT 72
5.2.2. ALLELE AND GENOTYPE FREQUENCY 73
5.2.3. FAMILY EFFECT ON GENOTYPE AND AVERAGE LITTER SIZE 74
5.2.4. ADDITIVE AND DOMINANCE EFFECT 75
5.3. ASSOCIATION ANALYSIS 77
5.3.1. CHI-SQUARES RESULTS FOR GENETIC ASSOCIATION 78
5.3.2. GENOTYPE-PHENOTYPE CORRELATION FOR LITTER SIZE 79
5.3.3. MARKER REGRESSION USING MAPPING SOFTWARE MAP MANAGER
QTXB20 79
5.4. PERMUTATION TESTS 81
5.5. USING KNOCKOUT MICE AS CONGENICS TO MAP LITTER SIZE QTLS 82
5.6. EFFICIENCY O F THE KNOCKOUT MICE MAPPING TECHNOLOGY 84
5.7. MARKER ASSISTED SELECTION 84
5.7.1. HOMOLOGOUS LOCI FOR FERTILITY BETWEEN MICE AND PIGS 85
6. SUMMARY. 87
7 LIST O F REFERENCES 92
8 APPENDICES 104
9 LIST O F TABLES 113
10 LIST O F FIGURES 114
ACKNOWLEDGEMENT 115
STATUTORY DECLARATION 116
5 |
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| dewey-full | 636.082 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 636 - Animal husbandry |
| dewey-raw | 636.082 |
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| dewey-tens | 630 - Agriculture and related technologies |
| discipline | Biologie Agrarwissenschaft Agrar-/Forst-/Ernährungs-/Haushaltswissenschaft / Gartenbau |
| edition | Aufl. 2012 |
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| spelling | Gordon, Hadenal 1958- Verfasser (DE-588)1021286613 aut Association analysis of selected markers with litter size in mice Hadenal Gordon Aufl. 2012 Berlin Köster 2012 117 S. Ill. txt rdacontent n rdamedia nc rdacarrier Schriftenreihe Agrarwissenschaft 29 Zsfassung in dt. Sprache Zugl.: Berlin, Humboldt-Univ., Diss., 2011 Tiermodell (DE-588)4140660-6 gnd rswk-swf Wurfgröße (DE-588)4372234-9 gnd rswk-swf Markierungsgen (DE-588)4168918-5 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Wurfgröße (DE-588)4372234-9 s Markierungsgen (DE-588)4168918-5 s Tiermodell (DE-588)4140660-6 s DE-604 Schriftenreihe Agrarwissenschaft 29 (DE-604)BV010211734 29 X:MVB text/html http://deposit.dnb.de/cgi-bin/dokserv?id=3992676&prov=M&dok_var=1&dok_ext=htm Inhaltstext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024852326&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Gordon, Hadenal 1958- Association analysis of selected markers with litter size in mice Schriftenreihe Agrarwissenschaft Tiermodell (DE-588)4140660-6 gnd Wurfgröße (DE-588)4372234-9 gnd Markierungsgen (DE-588)4168918-5 gnd |
| subject_GND | (DE-588)4140660-6 (DE-588)4372234-9 (DE-588)4168918-5 (DE-588)4113937-9 |
| title | Association analysis of selected markers with litter size in mice |
| title_auth | Association analysis of selected markers with litter size in mice |
| title_exact_search | Association analysis of selected markers with litter size in mice |
| title_full | Association analysis of selected markers with litter size in mice Hadenal Gordon |
| title_fullStr | Association analysis of selected markers with litter size in mice Hadenal Gordon |
| title_full_unstemmed | Association analysis of selected markers with litter size in mice Hadenal Gordon |
| title_short | Association analysis of selected markers with litter size in mice |
| title_sort | association analysis of selected markers with litter size in mice |
| topic | Tiermodell (DE-588)4140660-6 gnd Wurfgröße (DE-588)4372234-9 gnd Markierungsgen (DE-588)4168918-5 gnd |
| topic_facet | Tiermodell Wurfgröße Markierungsgen Hochschulschrift |
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