Kidney development: methods and protocols
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Humana Press
2012
|
| Schriftenreihe: | Methods in molecular biology
886 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XIV, 415 S. Ill., graph. Darst. |
| ISBN: | 9781617798504 |
Internformat
MARC
| LEADER | 00000nam a2200000 cb4500 | ||
|---|---|---|---|
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| 020 | |a 9781617798504 |c hardback |9 978-1-61779-850-4 | ||
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| 245 | 1 | 0 | |a Kidney development |b methods and protocols |c ed. by Odyssé Michos |
| 264 | 1 | |a New York, NY |b Humana Press |c 2012 | |
| 300 | |a XIV, 415 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 886 | |
| 490 | 0 | |a Springer protocols | |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-024849554 | ||
Datensatz im Suchindex
| _version_ | 1804148981877440512 |
|---|---|
| adam_text | Contents
Preface
..........................................................
v
Contributors.
..................................................... xi
Part I Organ Culture, Tissue Manipulation, and Live Imaging
1
Dissecting and Culturing and Imaging the Mouse
Urogenital
System
....... 3
Paul
N.
Riccio
and
Odyssé
Michos
2
In Vitro Culture of Embryonic Kidney Rudiments
and Isolated Ureteric Buds
....................................... 13
Xing Zhang, Kevin T. Bush, and Sanjay K. Nigam
3
In Vitro Induction of Nephrogenesis in Mouse Metanephric
Mesenchyme with Lithium Introduction and Ureteric
Bud Recombination
............................................ 23
Kimmo
Halt and Seppo Vainio
4
Live Imaging of the Developing Mouse Mesonephros
................... 31
David
Grote,
Michael
Marcotte,
and
Maxime
Bouchard
5
Organotypic Culture of the
Urogenital
Tract
.......................... 45
Ekaterina Batourina,
Levantini
Gandhi, Cathy L. Mendelsohn,
and Andrei Molotkov
6
Live Imaging Kidney Development in Zebrafish
....................... 55
Aleksandr
Vasilyev and Iain A.
Drummond
Part II Analyzing Ureteric Bud Branching and Nephrogenesis
7
Analysis of
3D
Branching Pattern: Hematoxylin and Eosin Method
......... 73
Sunder Sims-Lucas
8
Three-Dimensional Imaging of Fetal Mouse Kidneys
.................... 87
Deborah Hyink
9
Analysis of Native Kidney Structures in Three Dimensions
............... 95
Kieran M. Short and Ian M. Smyth
10
Estimating Nephron Number in the Developing Kidney Using
the Physical Disector/Fractionator Combination
....................... 109
Luise
A. Cullen-McEwen, James
A. Armitage,
Jens R. Nyengaard, and John F. Bertram
11
An Immunofluorescence Method to Analyze the Proliferation
Status of Individual Nephron Segments in the Xenopus
Pronephric Kidney
............................................. 121
Daniel Romaker, Bo Zhang, and Oliver Wessely
VII
viii Contents
Part III Renal
Primary Cell Cultures and Cell Lines
12
Dissociation of Embryonic Kidney Followed by Re-aggregation
as a Method for Chimeric Analysis
.................................. 135
Jamie A. Davies,
Mathieu
Unbekandt, Jessica Ineson,
Michael Lusts, and Melissa H. Little
13
Analysis of Migration in Primary Ureteric Bud Epithelial Cells
............. 147
Satu
Kuure
14
Investigating Primary Cilia in Cultured Metanephric
Mesenchymal Cells
............................................. 157
Lijun Chi and Norman
Rosenblum
15
Making Immortalized Cell Lines from Embryonic Mouse Kidney
.......... 165
Guanping
Tai,
Peter Hohenstein, and Jamie A. Davies
16
Engineered Tissues to Quantify Collective Cell Migration
During Morphogenesis
.......................................... 173
Sriram Manivannan, Jason P. Gleghorn, and Celeste M. Nelson
Part IV Detecting Gene/Protein Expression and Signaling
17
Access and Use of the GUDMAP Database
of Genitourinary Development
.................................... 185
Jamie A. Davies, Melissa H. Little, Bruce
Arenavo,
Jane Armstrong,
Jane Brennan, Sue Lloyd-MacGilp, Chris Armit, Simon Harding,
Xinjun
Piu,
Yogmatee Roochun, Bernard Haggarty,
Derek Houghton, Duncan Davidson, and Richard Baldock
18
Isolation of High Quality
RNA
from Embryonic Kidney and Cells
......... 203
Shifaan Thowfeequ and
Odyssé Michos
19
Laser Capture
................................................. 211
S. Steven Potter and Eric W. Brunskill
20
Use of In Situ Hybridization to Examine Gene Expression
in the Embryonic, Neonatal, and Adult
Urogenital
System
............... 223
Bree
A. Rumballe, Han Sheng
Chiu,
Kylie
M. Georgas,
and Melissa H. Little
21
Detection of
ß-Galactosidase
Activity:
Х
-gal
Staining
.................... 241
Sally F. Burn
22
Fluorescent Immunolabeling of Embryonic Kidney Samples
.............. 251
Cristina Cebrián
23
Immunohistochemical Staining of dpERK Staining During
Early Kidney Development
....................................... 261
Xuan Chi and
Odyssé Michos
24
Sensing BMP Pathway Activity by Immune Detection
of Phosphorylated R-Smad Proteins in Mouse Embryonic Kidney
.......... 267
Javier Lopez-Rios
25
Analysis of In Vivo Transcription Factor Recruitment by Chromatin
Immunoprecipitation of Mouse Embryonic Kidney
..................... 275
Claire Heliot and Silvia Cereghini
Contents
¡χ
Part V Manipulating Gene Expression in Developing
and Adult Kidney
26
siRNA-Mediated
RNA
Interference in Embryonic Kidney
Organ Culture
................................................ 295
Jamie A. Davies and
Mathieu Unbekandt
27
Morpholino-Mediated Gene Knockdown in Mammalian
Organ Culture
................................................ 305
Alda Tufro
28
Microinjection
into the Lumen of the Ureteric Tree
.................... 311
Cristina Cebrián
29
Renal Delivery of Adenovirus and Antisense Oligonucleotides
in Rats by Retrograde Renal Vein Injection
........................... 321
Guadalupe Ortiz-Muñoz, Beñat Mallavia,
Oscar Lopez-Franco,
Purificación
Hernandez-Vargas, Jesus
Egido,
and Carmen Gomez-Guerrero
Part VI Analyzing Functional Defects of the Kidney
and Urinary Tract
30
Estimating Total Nephron Number in the Adult Kidney
Using the Physical Disector/Fractionator Combination
.................. 333
Luise
A. Cullen-McEwen, Rebecca
N.
Douglas-Denton,
and John
F
Bertram
31
Assessing Urinary Tract Defects in Mice: Methods to Detect
the Presence ofVesicoureteric Reflux and Urinary Tract Obstruction
....... 351
Inga
J
.
Murawski, Christine L. Watt, and
Indra R.
Gupta
32
Ischemia-Reperfusion Injury of the Mouse Kidney
..................... 363
LeifOxburgh and Mark P.
de Caestecker
33
Variable Partial Unilateral
Ureteral
Obstruction and Its Release
in the Neonatal and Adult Mouse
.................................. 381
Barbara A. Thornhill and Robert L. Chevalier
34
Urinary Diversion via Cutaneous Vesicostomy
in the Megabladder Mouse
....................................... 393
Ashley R. Carpenter, Brian Becknell, Daniel A. Hirselj,
and Kirk M. McHugh
35
Ultrasound Imaging of the
Murine
Kidney
........................... 403
Ashley R. Carpenter, Brian Becknell, Susan E. Ingraham,
and Kirk M. McHugh
Index
........................................................... 411
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| indexdate | 2024-07-10T00:15:46Z |
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| isbn | 9781617798504 |
| language | English |
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| series | Methods in molecular biology |
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| spelling | Kidney development methods and protocols ed. by Odyssé Michos New York, NY Humana Press 2012 XIV, 415 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 886 Springer protocols Niere (DE-588)4042270-7 gnd rswk-swf Entwicklungsbiologie (DE-588)4152440-8 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Niere (DE-588)4042270-7 s Entwicklungsbiologie (DE-588)4152440-8 s b DE-604 Michos, Odyssé Sonstige oth Methods in molecular biology 886 (DE-604)BV035362695 886 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024849554&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Kidney development methods and protocols Methods in molecular biology Niere (DE-588)4042270-7 gnd Entwicklungsbiologie (DE-588)4152440-8 gnd |
| subject_GND | (DE-588)4042270-7 (DE-588)4152440-8 (DE-588)4143413-4 |
| title | Kidney development methods and protocols |
| title_auth | Kidney development methods and protocols |
| title_exact_search | Kidney development methods and protocols |
| title_full | Kidney development methods and protocols ed. by Odyssé Michos |
| title_fullStr | Kidney development methods and protocols ed. by Odyssé Michos |
| title_full_unstemmed | Kidney development methods and protocols ed. by Odyssé Michos |
| title_short | Kidney development |
| title_sort | kidney development methods and protocols |
| title_sub | methods and protocols |
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| topic_facet | Niere Entwicklungsbiologie Aufsatzsammlung |
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