Protein engineering for therapeutics: B
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Amsterdam [u.a.]
Elsevier, Acad. Press
2012
|
| Ausgabe: | 1. ed. |
| Schriftenreihe: | Methods in enzymology
503 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XLIV, 348 S. Ill., graph. Darst. |
| ISBN: | 9780123969620 |
Internformat
MARC
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| 245 | 1 | 0 | |a Protein engineering for therapeutics |n B |c ed. by K. Dane Wittrup ... |
| 250 | |a 1. ed. | ||
| 264 | 1 | |a Amsterdam [u.a.] |b Elsevier, Acad. Press |c 2012 | |
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Datensatz im Suchindex
| _version_ | 1804148946500583424 |
|---|---|
| adam_text | Contents
Contributors
ix
Preface
xiii
Volumes in series
xv
Section IV. Peptides
1
1.
Stapled Peptides for Intracellular Drug Targets
3
Gregory L.
Verdine
and Gerard J. Hilinski
1.
Introduction
4
2.
All-Hydrocarbon Stapled
α
-Helical Peptides
6
3.
The Design of Stapled Peptides
9
4.
Stapled
Peptide
Synthesis
12
5.
Olefin
Metathesis
16
6.
N-terminal and Internal Modifications
16
7.
Cleavage of Stapled Peptides from the Solid Support
19
8.
Stapled
Peptide
Purification
20
9.
Biophysical Characterization
20
10.
Cell Permeability
23
11.
In Vitro Target Interaction and Activity Assays
26
12.
In Vivo Efficacy
27
13.
Strategies for Stapled
Peptide
Optimization
28
14.
Summary
30
Acknowledgments
30
References
30
2.
Mapping of Vascular ZIP Codes by Phage Display
35
Tambet Teesalu, Kazuki
N.
Sugahara, and
Erkki Ruoslahti
1.
Introduction
36
2.
Methods
41
3.
Concluding Remarks and Perspectives
52
Acknowledgments
53
References
53
vi
Contents
3.
Engineering
Cyclic
Peptide
Toxins
57
Richard J. Clark and David J. Craik
1.
Introduction
58
2.
Peptide
Design
59
3.
Peptide
Synthesis
60
4.
Structural Analysis
66
5.
Stability Assays
68
6.
Special Considerations for Cyclic Peptides
69
Acknowledgments
71
References
71
4.
Peptide
Discovery Using Bacterial Display and Flow Cytometry
75
Jennifer A. Getz, Tobias D. Schoep, and Patrick S. Daugherty
1.
Introduction
76
2.
Protocols
79
3.
Conclusions
94
Acknowledgments
95
References
95
Section V. Scaffolds
99
5.
Designed Ankyrin Repeat Proteins (DARPins): From Research
to Therapy
101
Rastislav Tamaskovic,
Manuel Simon, Nikolas Stefan, Martin
Schwill,
and Andreas Pliickthun
1.
Introduction
102
2.
Applications of DARPins
107
3.
Protocols for DARPins in
Biomedical
Applications
112
Acknowledgment
129
References
129
6.
Target-Binding Proteins Based on the 10th Human Fibronectin
Type III Domain (10Fn3)
135
Shohei Koide, Akiko Koide, and
Dáša Lipovšek
1.
Introduction
136
2.
Library Design
137
3.
Choice of Selection Platform
139
4.
Phage Display, mRNA Display, and Yeast-Surface Display
of 10Fn3-Based Libraries
141
5.
Production
151
Contents
vii
6.
Conclusion
153
Acknowledgments
154
References
154
7.
Anticalins: Small Engineered Binding Proteins Based on the
Lipocalin Scaffold
157
Michaela Gebauer
and
Arne Skerra
1.
Introduction
158
2.
Cloning and Expression of Lipocalins and Anticalins in E. coll
166
3.
Construction of a Genetic Anticalin Library
170
4.
Preparation and Selection of a Phage Display Library for Anticatins
172
5.
Preparation and Selection of a Bacterial Surface Display Library
for Anticalins
176
6.
Colony Screening for Anticalins with Specific Target-Binding Activity
177
7.
Screening for Anticalins with Specific Target-Binding Activity Using
Microtiter Plate Expression in
£
coli
179
8.
Measuring Target Affinity of Anticalins in an
ELISA
181
9.
Measuring Target Affinity of Anticalins via Surface Plasmon Resonance
182
10.
Application of Anticalins in Biochemical Research and
Drug Development
184
References
186
8.
Τ
Cell Receptor Engineering
189
Jennifer D. Stone, Adam S. Chervin, David H.
Aggen,
and David M.
Kranz
1.
Introduction
190
2.
Stability and Affinity Engineering of
T
Cell Receptors by Yeast
Surface Display
192
3.
Affinity Engineering and Selection of
T
Cell Receptors
by
T
Cell Display
200
4.
Expression, Purification, and Applications of Soluble scTv Proteins
207
5.
Recipes for Media and Buffers
215
Acknowledgments
218
References
219
9.
Engineering Knottins as Novel Binding Agents
223
Sarah
).
Moore and Jennifer R. Cochran
1.
Introduction
224
2.
Knottins as Scaffolds for Engineering Molecular Recognition
225
3.
Engineering Knottins by Yeast Surface Display
227
4.
Knottin Library Construction
230
5.
Screening Yeast-Displayed Knottin Libraries
234
6.
Knottin production by chemical synthesis or
recombinant
expression
237
7.
Cell binding assays
243
viii Contents
8.
Summary
247
Acknowledgments
247
References
247
Section VI. Pharmacokinetics
253
10.
Practical Theoretic Guidance for the Design of
Tumor-Targeting Agents
255
K. Dane Wittrup, Greg M. Thurber, Michael M. Schmidt, and John
).
Rhoden
1.
Introduction
256
2.
What Molecular Size Is Best for Tumor Uptake?
256
3.
Will Targeting Increase Nanoparticle Accumulation in a Tumor?
259
4.
How Does Affinity Affect
Biodistribution?
261
5.
What Dose Is Necessary in Order to Overcome the Binding
Site Barrier ?
263
6.
Conclusions
264
References
265
11.
Reengineering Biopharmaceuticals
for Targeted Delivery Across
the Blood-Brain Barrier
269
William M. Pardridge and Ruben
].
Boado
1.
Introduction
270
2.
Blood-Brain Barrier Receptor-Mediated Transport and Molecular
Trojan Horses
271
3.
Reengineering Recombinant
Proteins for Targeted Brain Delivery
274
4.
Genetic Engineering of Expression Plasmid
DNA
Encoding IgG
Fusion Proteins
278
5.
Pharmacokinetics and Brain Uptake of IgG Fusion Proteins
279
6.
CNS Pharmacological Effects of IgG Fusion Proteins
284
7.
Immune Response Against IgG Fusion Proteins
287
8.
Summary
288
References
289
12.
Engineering and Identifying Supercharged Proteins for
Macromolecule
Delivery into Mammalian Cells
293
David B. Thompson, James J. Cronican, and David R. Liu
1.
Introduction
294
2.
Methods
301
3.
Conclusion
317
References
318
Author Index 321
Subject Index
jaj
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| indexdate | 2024-07-10T00:15:13Z |
| institution | BVB |
| isbn | 9780123969620 |
| language | English |
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| physical | XLIV, 348 S. Ill., graph. Darst. |
| publishDate | 2012 |
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| series | Methods in enzymology |
| series2 | Methods in enzymology |
| spelling | Protein engineering for therapeutics B ed. by K. Dane Wittrup ... 1. ed. Amsterdam [u.a.] Elsevier, Acad. Press 2012 XLIV, 348 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in enzymology 503 Methods in enzymology ... Proteindesign (DE-588)4346801-9 gnd rswk-swf Biochemische Methode (DE-588)4319880-6 gnd rswk-swf Therapie (DE-588)4059798-2 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Proteindesign (DE-588)4346801-9 s Biochemische Methode (DE-588)4319880-6 s DE-604 Therapie (DE-588)4059798-2 s b DE-604 Wittrup, K. Dane Sonstige oth (DE-604)BV039965761 2 Methods in enzymology 503 (DE-604)BV000000938 503 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024826311&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Protein engineering for therapeutics Methods in enzymology Proteindesign (DE-588)4346801-9 gnd Biochemische Methode (DE-588)4319880-6 gnd Therapie (DE-588)4059798-2 gnd |
| subject_GND | (DE-588)4346801-9 (DE-588)4319880-6 (DE-588)4059798-2 (DE-588)4143413-4 |
| title | Protein engineering for therapeutics |
| title_auth | Protein engineering for therapeutics |
| title_exact_search | Protein engineering for therapeutics |
| title_full | Protein engineering for therapeutics B ed. by K. Dane Wittrup ... |
| title_fullStr | Protein engineering for therapeutics B ed. by K. Dane Wittrup ... |
| title_full_unstemmed | Protein engineering for therapeutics B ed. by K. Dane Wittrup ... |
| title_short | Protein engineering for therapeutics |
| title_sort | protein engineering for therapeutics |
| topic | Proteindesign (DE-588)4346801-9 gnd Biochemische Methode (DE-588)4319880-6 gnd Therapie (DE-588)4059798-2 gnd |
| topic_facet | Proteindesign Biochemische Methode Therapie Aufsatzsammlung |
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