Protein engineering for therapeutics: A
Gespeichert in:
Format: | Buch |
---|---|
Sprache: | English |
Veröffentlicht: |
Amsterdam [u.a.]
Elsevier, Acad. Press
2012
|
Ausgabe: | 1. ed. |
Schriftenreihe: | Methods in enzymology
502 |
Schlagworte: | |
Online-Zugang: | Inhaltsverzeichnis |
Beschreibung: | XLIV, 344 S. Ill., graph. Darst. |
ISBN: | 9780124160392 |
Internformat
MARC
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245 | 1 | 0 | |a Protein engineering for therapeutics |n A |c ed. by K. Dane Wittrup ... |
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264 | 1 | |a Amsterdam [u.a.] |b Elsevier, Acad. Press |c 2012 | |
300 | |a XLIV, 344 S. |b Ill., graph. Darst. | ||
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Datensatz im Suchindex
_version_ | 1804148942405894144 |
---|---|
adam_text | Contents
Contributors
ix
Preface
xiii
Volumes in Series
xv
Section I. Antibodies
1
1.
Simplified Synthetic Antibody Libraries
3
Saravanan
Rajan
and Sachdev S. Sidhu
1.
Introduction
4
2.
Materials
6
3.
Methods
8
References
22
2.
Generation of Dual-Variable-Domain Immunoglobulin Molecules
for Dual-Specific Targeting
25
Jijie Gu and Tariq Ghayur
1.
Introduction
26
2.
Design of DVD-lg™ Molecules
28
3.
Cloning and Expression of DVD-lg™ Molecules
31
4.
Molecular Characterization of DVD-lg™ Proteins
36
5.
Functional Characterization of DVD-lg™ Proteins
38
6.
Concluding Remarks
40
Acknowledgments
40
References
40
3.
Discovery of Internalizing Antibodies to Tumor Antigens from
Phage Libraries
43
Yu Zhou and James D. Marks
1.
Introduction
44
2.
Selection of Tumor Cell-Specific Internalizing scFv from Phage
Antibody Libraries
48
3.
Profiling the Specificity of Phage Antibodies on Tumor Cells Using
Flow Cytometry
52
4.
Identification of the Cognate Antigen Recognized by Tumor-Specific
scFv by Using Mass Spectrometry
53
vi
Contents
5.
Selection of Antigen-Specific Phage Antibodies by Sequential
Selection on Tumor Cells Followed by Selection on Yeast Cells
Displaying Specific Tumor Antigens
58
References
63
4.
Optimizing Properties of Antireceptor Antibodies Using Kinetic
Computational Models and Experiments
67
Brian D. Harms, Jeffrey D. Kearns, Stephen V.
Su,
Neeraj Kohli,
Ulrik B.
Nielsen, and
Birgit Schoeberl
1.
Introduction: Valence, Affinity, and Avidity in Antibodies
68
2.
Quantifying Antibody Cross-linking Strength Using VFC Models
of Antibody Binding to Cell-Surface Receptors
69
3.
Workflow for Quantifying Antibody Cross-linking Strength
73
4.
Model Applications and Insights
78
Acknowledgments
86
References
86
Section II. Protein Conjugates
89
5.
Cell-Specific siRNA Delivery by Peptides and Antibodies
91
Sang-Kyung Lee, Alyssa Siefert, Jagadish Beloor,
Tarek
M. Fahmy,
and Priti Kumar
1.
Introduction
92
2.
Cationic Cell Permeable Peptides
92
3.
Solid Biodegradable Polymer and Vesicular (Liposomal) Systems
109
Acknowledgments
118
References
118
6.
Conjugation of
Anticancer
Drugs Through Endogenous
Monoclonal Antibody
Cysteine
Residues
123
Robert P.
Lyon,
David L. Meyer, Jocelyn R. Setter, and Peter D. Senter
1.
Antibody Reduction
124
2.
Monitoring Antibody Reduction
127
3.
Conjugation with Maleimido Drug-Linker
128
4.
Purification Methods
128
5.
Small-Scale Conjugations for Early ADC Discovery
129
6.
Procedure for Solid-Phase Conjugation
132
7.
Determination of Drug Loading
133
8.
Determination of Unconjugated Drug Content
136
9.
Conclusions
137
References
137
Contents
vii
7. Building
and Characterizing Antibody-Targeted
Lipidie
Nanotherapeutics
139
Dmitri B. Kirpotin, Charles
0.
Noble, Mark E. Hayes, Zhaohua Huang,
Tad Kornaga, Yu Zhou,
Ulrik
В.
Nielsen, James D. Marks,
and Daryt
С
Drummond
1.
Introduction
140
2.
Preparation of Immunoliposomes
143
3.
Physicochemical Characterization of Immunoliposomes
157
4.
In Vitro and In Vivo Characterization of Immunoliposomal
Therapeutics
160
References
164
8.
Cell-Targeting Fusion Constructs Containing
Recombinant
Gelonin
167
Mi-Ae Lyu, Yu Qoshua)
Cao, Khalid
A. Mohamedali,
and
Michael G.
Rosenblum
1.
Introduction
168
2.
Engineered Proteins Targeting Her2/neu
169
3.
rGel/BLyS Targeting Malignant B-Cells
181
4.
VEGFui/rGel Targeting Tumor Vasculature and Skeletal
Métastases
195
References
210
9.
Fusions of Elastin-Like Polypeptides to Pharmaceutical Proteins
215
Wafa Hassouneh, Sarah R. MacEwan, and Ash
u
tosh Chilkoti
1.
Introduction
216
2.
Protein ELP Fusion Design
218
3.
Recursive Directional Ligation by Plasmid Reconstruction
221
4.
Expression and Purification of Protein ELP Fusions
228
5.
Physical Characterization of Protein ELP Fusions
233
6.
Conclusions
235
Acknowledgment
236
References
236
Section III. ENZYMES
239
10.
Oral Enzyme Therapy for
Čeliac
Sprue
241
Michael T. Bethune and Chaitan Khosla
1.
Introduction
242
2.
Lead Identification
245
3.
Assay Development
248
4.
Gram-Scale Production and Formulation
259
5.
Lead Optimization for Next-Generation Proteases
261
viii Contents
6.
Summary and Outlook
264
References
266
11.
Rational Design and Evaluation of Mammalian Ribonuclease
Cytotoxins
273
Jo E. Lomax, Chelcie H.
Eller,
and Ronald T. Raines
1.
Introduction
274
2.
Attributes of Cytotoxic ptRNases
276
3.
Assays to Evaluate the Cytotoxicity of ptRNases
280
4.
Prospectus
287
Acknowledgments
287
References
287
12.
Engineering Reduced-lmmunogenicity Enzymes for
Amino
Acid
Depletion Therapy in Cancer
291
Jason R. Cantor, Vasiliki Panayiotou,
Giulia Agnello,
George Georgiou,
and Everett M. Stone
1.
Introduction:
Amino
Acid Depletion as a Chemotherapeutic Strategy
292
2.
Method I. Combinatorial Deimmunization
297
3.
Method II. Engineering Therapeuticatly Relevant Substrate
Specificities into a Human Enzyme
302
4.
Method III. Improving the Kinetics and Stability of a Human Enzyme
309
5.
Concluding Remarks
312
References
314
Author Index
321
Subject Index
339
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illustrated | Illustrated |
indexdate | 2024-07-10T00:15:09Z |
institution | BVB |
isbn | 9780124160392 |
language | English |
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physical | XLIV, 344 S. Ill., graph. Darst. |
publishDate | 2012 |
publishDateSearch | 2012 |
publishDateSort | 2012 |
publisher | Elsevier, Acad. Press |
record_format | marc |
series | Methods in enzymology |
series2 | Methods in enzymology |
spelling | Protein engineering for therapeutics A ed. by K. Dane Wittrup ... 1. ed. Amsterdam [u.a.] Elsevier, Acad. Press 2012 XLIV, 344 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in enzymology 502 Methods in enzymology ... Therapie (DE-588)4059798-2 gnd rswk-swf Biochemische Methode (DE-588)4319880-6 gnd rswk-swf Proteindesign (DE-588)4346801-9 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Proteindesign (DE-588)4346801-9 s Therapie (DE-588)4059798-2 s DE-604 Biochemische Methode (DE-588)4319880-6 s b DE-604 Wittrup, K. Dane Sonstige oth (DE-604)BV039965761 1 Methods in enzymology 502 (DE-604)BV000000938 502 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024823416&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
spellingShingle | Protein engineering for therapeutics Methods in enzymology Therapie (DE-588)4059798-2 gnd Biochemische Methode (DE-588)4319880-6 gnd Proteindesign (DE-588)4346801-9 gnd |
subject_GND | (DE-588)4059798-2 (DE-588)4319880-6 (DE-588)4346801-9 (DE-588)4143413-4 |
title | Protein engineering for therapeutics |
title_auth | Protein engineering for therapeutics |
title_exact_search | Protein engineering for therapeutics |
title_full | Protein engineering for therapeutics A ed. by K. Dane Wittrup ... |
title_fullStr | Protein engineering for therapeutics A ed. by K. Dane Wittrup ... |
title_full_unstemmed | Protein engineering for therapeutics A ed. by K. Dane Wittrup ... |
title_short | Protein engineering for therapeutics |
title_sort | protein engineering for therapeutics |
topic | Therapie (DE-588)4059798-2 gnd Biochemische Methode (DE-588)4319880-6 gnd Proteindesign (DE-588)4346801-9 gnd |
topic_facet | Therapie Biochemische Methode Proteindesign Aufsatzsammlung |
url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024823416&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
volume_link | (DE-604)BV039965761 (DE-604)BV000000938 |
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