Recombinant gene expression: reviews and protocols
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York [u.a.]
Humana Press
2012
|
| Ausgabe: | 3. ed. |
| Schriftenreihe: | Methods in molecular biology
824 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XVI, 649 S. Ill., graph. Darst. |
| ISBN: | 1617794325 9781617794322 |
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Datensatz im Suchindex
| _version_ | 1804148868523229184 |
|---|---|
| adam_text | Recombinant Gene Expression
Reviews and Protocols, Third Edition
Edited by
Argelia Lorence
Arkansas Biosciences Institute, Arkansas State University, Jonesboro, AR, USA
Humana Press
Contents
Preface vii
Contributors xiii
PART I GENERAL ASPECTS
1 Using Folding Promoting Agents in Recombinant
Protein Production: A Review 3
Beatrix Fabnert
2 Routine Identity Confirmation of Recombinant Proteins
by MALDI-TOF Mass Spectrometry 37
Brett J Savary and Prasanna Vast*
3 A Matter of Packaging: Influence of Nucleosome Positioning
on Heterologous Gene Expression 51
Maria tie la Crttz Muhoz-Centeno, Gonzalo Milldn-Zambrano,
and Sebastian Chavez
4 Tools of the Trade: Developing Antibody-Based Detection
Capabilities for Recombinant Proteins 65
Maureen C Dolan, Giuliana Medrano, Anthony McMickle,
and Carole L Cramer
PART II PROKARYOTKS
5 Heat-Shock Protein Fusion Vectors for Improved Expression
of Soluble Recombinant Proteins in Escherichia coli 109
Christos A Kyratsous and Christos A Panagiotidi-
6 The Use of a Flagellar Export Signal for the Secretion
of Recombinant Proteins in Salmonella 131
Ferenc Vonderviszt, Rachel Sajd, Jozsef Dobo, and Peter Zdvodszky
7 Optimization of Purification Protocols Based on the Step-by-Step
Monitoring of the Protein Aggregates in Soluble Fractions 145
Ario de Marco
8 Heterologous Protein Expression by Lactococcus lactis 155
Julio Villatoro-Herndndez, Oscar P Kuipers,
Odila Saucedo-Cdrdenas, and Roberto Montes-de-Oca-Luna
9 An Extended Suite of Genetic Tools for Use in Bacte ria
of the Halomonadaceae An Overview 167
Montserrat Argandoha, Carmen Vargas, Mercedes Reina-Bueno,
Javier Rodriguez-Moya, Manuel Salvador, and Joaquin J Nieto
ix
X Contents
10 Regulated Recombinant Protein Production in the Antarctic Bacterium
Pseudoalteromonas haloplanktisTACl25 203
Valentina Rippa, Rosanna Papa, Maria Giuliani, Cinzia Pezzella,
Ermenegilda Parrilli, Maria Luisa Tutino, Gennaro Marino,
and Angela Duilio
11 A Novel Strategy for the Construction of Genomic Mutants
of the Antarctic Bacterium Pseudoalteromonas haloplanktisTAC 125 219
Maria Giuliani, Ermenejjilda Parrilli, Cinzia Pezzella, Valentina Rippa,
Angela Duilio, Gennaro Marino, and Maria Luisa Tutino
12 A New Bacterial Co-expression System for Over-expressing Soluble Protein
and Validating Protein-Protein Interaction 235
Jumei Zeng and Zheng-Guo He
13 Heterologous High-Level Gene Expression in the Photosynthetic
Bacterium Rhodobacter capsulatus 251
Nadine Katzke, Rene Bergmann, Karl-Erich Jaeger, and Thomas Drepper
14 Plasmid DNA Production for Therapeutic Applications 271
Alvaro R Lara and Octavio T Ramirez
PART III LOWER EUKARYOTES
15 Recombinant Protein Production in the Eukaryotic Protozoan Parasite
Leishmania tarentolae: A Review
Tomoaki Niimi
16 Expression of Multisubunit Proteins in Leishmania tarentolae
Marisa Sugino and Tomoaki Niimi
PART IV FUNGI
17 Recombinant Protein Production in Yeasts 329
Diethard Mattanovich, Paola Branduardi, Laura Data, Brigitte Gasser,
Michael Sauer, and Danilo Porro
18 Yeasts as a Tool for Heterologous Gene Expression 359
Raja Mokdad-Gargouri, Salma Abdelmoula-Soussi, Nadia Hadiji-Abbes,
Ines Tacoubi-Hadj Amor, Istabrak Borchani-Chabchoub, and Ali Gargouri
19 The Cre/Lox System: A Practical Tool to Efficiently Eliminate Selectable
Markers in Fungal Endophytes 371
Simona Florea, Caroline Machado, Kalina Andreeva,
and Christopher L Schardl
20 Aptamer-Regulated Expression of Essential Genes in Yeast 381
Beatrix Suess, Karl-Dieter Entian, Peter Kotter, and Julia E Weigand
21 Cloning and Expression of Hemicellulases from Aspergillus nidulans
in Pichia pastoris 393
Prasanna Vasu, Stefan Bauer, and Brett J Savary
22 A Thiamine-Regulatable Epitope-Tagged Protein Expression System
in Fission Yeast 417
Tiina Tamm
Contents xi
23 Heterologous Gene Expression by Chromosomal Integration
in Fission Yeast 433
Akihisa Matsuyama and Minoru Toshida
24 Genetic Engineering of Industrial Strains of Sac charomyces cerevisiae 451
Sylvie Le Borgne
PART V PLANTS AND PLANT CELLS
25 Recombinant Protein Production in Plants: Challenges and Solutions
Elizabeth E Hood and Deborah V Vicuna Ret; uesens
26 A Novel Plant Cell Bioproduction Platform for High-Yield Secretion
of Recombinant Proteins
Jianfeng Xu and Marcia J Kieliszewski
27 Super-promoter:TEV, a Powerful Gene Expression System
for Tobacco Hairy Roots
Luis Nopo, Bonnie J Woffenden, Deborah G Reed, Scott Buswell,
Chenming Zhang, and Fabricio Medina-Bolivar
28 Bioseparation of Recombinant Proteins from Plant Extract
with Hydrophobin Fusion Technology
Jussi J Joensuu, Andrew J Conley, Markus B Linder, and Rima Menassa
29 Quality Assessment of Recombinant Proteins Produced in Plants
Giuliana Medrano, Maureen C Dolan, Jose Condori, David N Radin,
and Carole L Cramer
30 Cell-Free Protein Synthesis as a Promising Expression System
for Recombinant Proteins
Xumeng Ge and Jianfeng Xu
PART VI ANIMALS AND ANIMAL CELLS
31 The Use of Bacterial Artificial Chromosomes for Recombinant Protein
Production in Mammalian Cell Lines 581
Leander Blaas, Monica Musteanu, Beatrice Grabner, Robert Eferl,
Anton Bauer, and Emilio Casanova
32 Engineering the Chaperone Network of CHO Cells for Optimal Recombinant
Protein Production and Authenticity 595
Lyne Josse, C Mark Smales, and Mick F Tuite
33 High-Throughput Baculovirus Expression in Insect Cells 609
Richard B Hitchman, Robert D Possee, and Linda A King
34 Recombinant Protein Expression in Milk of Livestock Species 629
Zsuzsanna Bosze and Laszlo Hiripi
Index 643
|
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| discipline | Biologie |
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| title | Recombinant gene expression reviews and protocols |
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| title_exact_search | Recombinant gene expression reviews and protocols |
| title_full | Recombinant gene expression reviews and protocols ed. by Argelia Lorence |
| title_fullStr | Recombinant gene expression reviews and protocols ed. by Argelia Lorence |
| title_full_unstemmed | Recombinant gene expression reviews and protocols ed. by Argelia Lorence |
| title_short | Recombinant gene expression |
| title_sort | recombinant gene expression reviews and protocols |
| title_sub | reviews and protocols |
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