mTor: methods and protocols
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York
Humana Press
2012
|
| Schriftenreihe: | Methods in molecular biology
821 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Includes bibliographical reference and index Mammalian target of rapamycin : a signaling kinase for every aspect of cellular life / Thomas Weichhart -- Biochemical and pharmacological inhibition of mTOR by rapamycin and an ATP-competitive mTOR inhibitor / Ker Yu and Lourdes Toral-Barza -- Evaluation of the nutrient-sensing mTOR pathway / Sungki Hong, Aristotle M. Mannan, and Ken Inoki -- mTor activity under hypoxia / Douangsone D. Vadysirisack and Leif W. Ellisen -- Isolation of the mTOR complexes by affinity purification / Dos D. Sarbassov ... [et al.] -- An in vitro assay for the kinase activity of mTOR complex 2 / Jingxiang Huang -- Overexpression or downregulation of mTOR in mammalian cells / Mahmoud Khalil and Ivan Gout -- Detection of cytoplasmic and nuclear functions of mTOR by fractionation / Margit Rosner and Markus Hengstschläger -- Evaluation of rapamycin-induced cell death / Lorenzo Galluzzi ... [et al.] -- Evaluation of mTOR-regulated mRNA translation / Valentina Iadevaia ... [et al.] -- A genome-wide RNAi screen for polypeptides that alter rpS6 phosphorylation / Angela Papageorgiou and Joseph Avruch -- Immunohistochemical analysis of mTOR activity in tissues / Jinhee Kim ... [et al.] -- Assessing cell size and cell cycle regulation in cells with altered TOR activity / Megan Cully and Julian Downward -- Quantitative visualization of autophagy induction by mTOR inhibitors / Beat Nyfeler ... [et al.] -- The in vivo evaluation of active-site TOR inhibitors in models of BCR-ABL+ leukemia / Matthew R. Janes and David A. Fruman -- Inducible raptor and rictor knockout mouse embryonic fibroblasts / Nadine Cybulski, Vittoria Zinzalla, and Michael N. Hall -- Expanding human T regulatory cells with the mTOR-inhibitor rapamycin / Manuela Battaglia, Angela Stabilini, and Eleonora Tresoldi -- Rapamycin-induced enhancement of vaccine efficacy in mice / Chinnaswamy Jagannath and Pearl Bakhru -- Utilizing a retroviral RNAi system to investigate in vivo mTOR functions in T cells / Koichi Araki and Bogumila T. Konieczny -- Exploring functional in vivo consequences of the selective genetic ablation of mTOR signaling in T helper lymphocytes / Greg M. Delgoffe and Jonathan D. Powell -- Evaluating the therapeutic potential of mTOR inhibitors using mouse genetics / Huawei Li, Jennifer L. Cotton, and David A. Guertin -- Inhibition of PI3K-Akt-mTOR signaling in glioblastoma by mTORC1/2 inhibitors / Qi-Wen Fan and William A. Weiss -- Assessing the function of mTOR in human embryonic stem cells / Jiaxi Zhou, Dong Li, and Fei Wang -- Video-EEG monitoring methods for characterizing rodent models of tuberous sclerosis and epilepsy / Nicholas R. Rensing, Dongjun Guo, and Michael Wong -- A genetic model to dissect the role of TSC-mTORC1 in neuronal cultures / Duyu Nie and Mustafa Sahin -- Tissue-specific ablation of TSC1 in pancreatic beta-cells / Hiroyuki Mori and Kun-Liang Guan -- A mouse model of diet-induced obesity and insulin resistance / Chao-Yung Wang and James K. Liao -- Rapamycin as immunosuppressant in murine transplantation model / Louis-Marie Charbonnier and Alain Le Moine -- Development of ATP-competitive mTOR inhibitors / Qingsong Liu ... [et al.] |
| Beschreibung: | XIII, 464 S. Ill., graph. Darst. 26 cm |
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| 300 | |a XIII, 464 S. |b Ill., graph. Darst. |c 26 cm | ||
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| 490 | 1 | |a Methods in molecular biology |v 821 | |
| 490 | 0 | |a Springer protocols | |
| 500 | |a Includes bibliographical reference and index | ||
| 500 | |a Mammalian target of rapamycin : a signaling kinase for every aspect of cellular life / Thomas Weichhart -- Biochemical and pharmacological inhibition of mTOR by rapamycin and an ATP-competitive mTOR inhibitor / Ker Yu and Lourdes Toral-Barza -- Evaluation of the nutrient-sensing mTOR pathway / Sungki Hong, Aristotle M. Mannan, and Ken Inoki -- mTor activity under hypoxia / Douangsone D. Vadysirisack and Leif W. Ellisen -- Isolation of the mTOR complexes by affinity purification / Dos D. Sarbassov ... [et al.] -- An in vitro assay for the kinase activity of mTOR complex 2 / Jingxiang Huang -- Overexpression or downregulation of mTOR in mammalian cells / Mahmoud Khalil and Ivan Gout -- Detection of cytoplasmic and nuclear functions of mTOR by fractionation / Margit Rosner and Markus Hengstschläger -- Evaluation of rapamycin-induced cell death / Lorenzo Galluzzi ... [et al.] -- Evaluation of mTOR-regulated mRNA translation / Valentina Iadevaia ... [et al.] -- | ||
| 500 | |a A genome-wide RNAi screen for polypeptides that alter rpS6 phosphorylation / Angela Papageorgiou and Joseph Avruch -- Immunohistochemical analysis of mTOR activity in tissues / Jinhee Kim ... [et al.] -- Assessing cell size and cell cycle regulation in cells with altered TOR activity / Megan Cully and Julian Downward -- Quantitative visualization of autophagy induction by mTOR inhibitors / Beat Nyfeler ... [et al.] -- The in vivo evaluation of active-site TOR inhibitors in models of BCR-ABL+ leukemia / Matthew R. Janes and David A. Fruman -- Inducible raptor and rictor knockout mouse embryonic fibroblasts / Nadine Cybulski, Vittoria Zinzalla, and Michael N. Hall -- Expanding human T regulatory cells with the mTOR-inhibitor rapamycin / Manuela Battaglia, Angela Stabilini, and Eleonora Tresoldi -- Rapamycin-induced enhancement of vaccine efficacy in mice / Chinnaswamy Jagannath and Pearl Bakhru -- | ||
| 500 | |a Utilizing a retroviral RNAi system to investigate in vivo mTOR functions in T cells / Koichi Araki and Bogumila T. Konieczny -- Exploring functional in vivo consequences of the selective genetic ablation of mTOR signaling in T helper lymphocytes / Greg M. Delgoffe and Jonathan D. Powell -- Evaluating the therapeutic potential of mTOR inhibitors using mouse genetics / Huawei Li, Jennifer L. Cotton, and David A. Guertin -- Inhibition of PI3K-Akt-mTOR signaling in glioblastoma by mTORC1/2 inhibitors / Qi-Wen Fan and William A. Weiss -- Assessing the function of mTOR in human embryonic stem cells / Jiaxi Zhou, Dong Li, and Fei Wang -- Video-EEG monitoring methods for characterizing rodent models of tuberous sclerosis and epilepsy / Nicholas R. Rensing, Dongjun Guo, and Michael Wong -- A genetic model to dissect the role of TSC-mTORC1 in neuronal cultures / Duyu Nie and Mustafa Sahin -- Tissue-specific ablation of TSC1 in pancreatic beta-cells / Hiroyuki Mori and Kun-Liang Guan -- | ||
| 500 | |a A mouse model of diet-induced obesity and insulin resistance / Chao-Yung Wang and James K. Liao -- Rapamycin as immunosuppressant in murine transplantation model / Louis-Marie Charbonnier and Alain Le Moine -- Development of ATP-competitive mTOR inhibitors / Qingsong Liu ... [et al.] | ||
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-024754776 | ||
Datensatz im Suchindex
| _version_ | 1804148846575484928 |
|---|---|
| adam_text | Titel: mTOR
Autor: Weichhart, Thomas
Jahr: 2012
Contents
Preface.......................................................... v
Contributors...................................................... ix
1 Mammalian Target of Rapamycin: A Signaling Kinase for Every Aspect
of Cellular Life................................................ 1
Thomas Weichhart
2 Biochemical and Pharmacological Inhibition of mTOR by Rapamycin
and an ATP-Competitive mTOR Inhibitor........................... 15
Ker Yu and Lourdes Toral-Barza
3 Evaluation of the Nutrient-Sensing mTOR Pathway.................... 29
Sungki Hong, Aristotle M. Mannan, and Ken Inoki
4 mTOR Activity Under Hypoxia................................... 45
Douangsone D. Vadysirisack and Leif W. Ellisen
5 Isolation of the mTOR Complexes by Affinity Purification............... 59
DosD. Sarbassov, Olga Bulgakov a, Rakhmetl. Bersimbaev,
and Tattym Shaiken
6 An In Vitro Assay for the Kinase Activity of mTOR Complex 2............ 75
Jingxiang Huang
7 Overexpression or Downregulation of mTOR in Mammalian Cells......... 87
Mahmoud Khalil and Ivan Gout
8 Detection of Cytoplasmic and Nuclear Functions of mTOR by Fractionation . 105
Margit Rosner and Markus Hengstschlager
9 Evaluation of Rapamycin-Induced Cell Death......................... 125
Lorenzo Galluzzi, Eugenia Morselli, Oliver Kepp, Ilio Vitale,
Amena Ben Younes, Maria Chiara Maiuri, and Guido Kroemer
10 Evaluation of mTOR-Regulated mRNA Translation..................... 171
Valentina Iadevaia, Xuemin Wang, Zhong Yao, Leonard J. Foster,
and Christopher G. Proud
11 A Genome-wide RNAi Screen for Polypeptides that Alter
rpS6 Phosphorylation........................................... 187
Angela Papageorgiou and Joseph Avruch
12 Immunohistochemical Analysis of mTOR Activity in Tissues.............. 215
Jinhee Kim, Nancy Otto, ClaudioJ. Conti, Irma B. Gimenz-Conti,
and Cheryl L. Walker
13 Assessing Cell Size and Cell Cycle Regulation in Cells
with Altered TOR Activity....................................... 227
Megan Cully and Julian Downward
VII
viii Contents
14 Quantitative Visualization of Autophagy Induction by mTOR Inhibitors..... 239
BeatNyfeler, Philip Bergman, Christopher J. Wilson, and Leon O. Murphy
15 The In Vivo Evaluation of Active-Site TOR Inhibitors in Models
of BCR-ABL+ Leukemia......................................... 251
Matthew R. Janes and David A. Fruman
16 Inducible raptor and rictor Knockout Mouse Embryonic Fibroblasts........ 267
Nadine Cybulski, Vittoria Zinzalla, and Michael N. Hall
17 Expanding Human T Regulatory Cells with the mTOR-Inhibitor Rapamycin . 279
Manuela Battaglia, Angela Stabilini, and Eleonora Tresoldi
18 Rapamycin-Induced Enhancement of Vaccine Efficacy in Mice............ 295
Chinnaswamy Jagannath and Pearl Bakhru
19 Utilizing a Retroviral RNAi System to Investigate In Vivo mTOR
Functions in T Cells............................................ 305
Koichi Araki and Bqgumila T. Konieczny
20 Exploring Functional In Vivo Consequences of the Selective Genetic
Ablation of mTOR Signaling in T Helper Lymphocytes................. 317
Greg M. Delgojfe and Jonathan D. Powell
21 Evaluating the Therapeutic Potential of mTOR Inhibitors Using
Mouse Genetics............................................... 329
Huawei Li, Jennifer L. Cotton, and David A. Guertin
22 Inhibition of PI3K-Akt-mTOR Signaling in Glioblastoma
bymTORCl/2 Inhibitors....................................... 349
Qi-Wen Fan and William A. Weiss
23 Assessing the Function of mTOR in Human Embryonic Stem Cells........ 361
Jiaxi Zhou, Dong Li, and Fei Wang
24 Video-EEG Monitoring Methods for Characterizing Rodent Models
of Tuberous Sclerosis and Epilepsy................................. 373
Nicholas R. Rensing, Dongjun Guo, and Michael Wong
25 A Genetic Model to Dissect the Role of Tsc-mTORCl in Neuronal Cultures . 393
Duyu Nie and Mustafa Sahin
26 Tissue-Specific Ablation of Tscl in Pancreatic Beta-Cells................. 407
Hiroyuki Mori and Kun-Liang Guan
27 A Mouse Model of Diet-Induced Obesity and Insulin Resistance........... 421
Chao-Yung Wang and James K. Liao
28 Rapamycin as Immunosuppressant in Murine Transplantation Model....... 435
Louis-Marie Charbonnier and Alain Le Moine
29 Development of ATP-Competitive mTOR Inhibitors................... 447
QingsongLiu, SeongA. Kang, Carson C. Thoreen, Wooyoung Hur,
Jinhua Wang,Jae Won Chang, Andrew Markhard, Jianming Zhang,
Taebo Sim, David M. Sabatini, and Nathanael S. Gray
Index............................................................... 461
|
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| indexdate | 2024-07-10T00:13:37Z |
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| language | English |
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| physical | XIII, 464 S. Ill., graph. Darst. 26 cm |
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| spelling | mTor methods and protocols ed. by Thomas Weichhart New York Humana Press 2012 XIII, 464 S. Ill., graph. Darst. 26 cm txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 821 Springer protocols Includes bibliographical reference and index Mammalian target of rapamycin : a signaling kinase for every aspect of cellular life / Thomas Weichhart -- Biochemical and pharmacological inhibition of mTOR by rapamycin and an ATP-competitive mTOR inhibitor / Ker Yu and Lourdes Toral-Barza -- Evaluation of the nutrient-sensing mTOR pathway / Sungki Hong, Aristotle M. Mannan, and Ken Inoki -- mTor activity under hypoxia / Douangsone D. Vadysirisack and Leif W. Ellisen -- Isolation of the mTOR complexes by affinity purification / Dos D. Sarbassov ... [et al.] -- An in vitro assay for the kinase activity of mTOR complex 2 / Jingxiang Huang -- Overexpression or downregulation of mTOR in mammalian cells / Mahmoud Khalil and Ivan Gout -- Detection of cytoplasmic and nuclear functions of mTOR by fractionation / Margit Rosner and Markus Hengstschläger -- Evaluation of rapamycin-induced cell death / Lorenzo Galluzzi ... [et al.] -- Evaluation of mTOR-regulated mRNA translation / Valentina Iadevaia ... [et al.] -- A genome-wide RNAi screen for polypeptides that alter rpS6 phosphorylation / Angela Papageorgiou and Joseph Avruch -- Immunohistochemical analysis of mTOR activity in tissues / Jinhee Kim ... [et al.] -- Assessing cell size and cell cycle regulation in cells with altered TOR activity / Megan Cully and Julian Downward -- Quantitative visualization of autophagy induction by mTOR inhibitors / Beat Nyfeler ... [et al.] -- The in vivo evaluation of active-site TOR inhibitors in models of BCR-ABL+ leukemia / Matthew R. Janes and David A. Fruman -- Inducible raptor and rictor knockout mouse embryonic fibroblasts / Nadine Cybulski, Vittoria Zinzalla, and Michael N. Hall -- Expanding human T regulatory cells with the mTOR-inhibitor rapamycin / Manuela Battaglia, Angela Stabilini, and Eleonora Tresoldi -- Rapamycin-induced enhancement of vaccine efficacy in mice / Chinnaswamy Jagannath and Pearl Bakhru -- Utilizing a retroviral RNAi system to investigate in vivo mTOR functions in T cells / Koichi Araki and Bogumila T. Konieczny -- Exploring functional in vivo consequences of the selective genetic ablation of mTOR signaling in T helper lymphocytes / Greg M. Delgoffe and Jonathan D. Powell -- Evaluating the therapeutic potential of mTOR inhibitors using mouse genetics / Huawei Li, Jennifer L. Cotton, and David A. Guertin -- Inhibition of PI3K-Akt-mTOR signaling in glioblastoma by mTORC1/2 inhibitors / Qi-Wen Fan and William A. Weiss -- Assessing the function of mTOR in human embryonic stem cells / Jiaxi Zhou, Dong Li, and Fei Wang -- Video-EEG monitoring methods for characterizing rodent models of tuberous sclerosis and epilepsy / Nicholas R. Rensing, Dongjun Guo, and Michael Wong -- A genetic model to dissect the role of TSC-mTORC1 in neuronal cultures / Duyu Nie and Mustafa Sahin -- Tissue-specific ablation of TSC1 in pancreatic beta-cells / Hiroyuki Mori and Kun-Liang Guan -- A mouse model of diet-induced obesity and insulin resistance / Chao-Yung Wang and James K. Liao -- Rapamycin as immunosuppressant in murine transplantation model / Louis-Marie Charbonnier and Alain Le Moine -- Development of ATP-competitive mTOR inhibitors / Qingsong Liu ... [et al.] Rapamycin / Laboratory manuals Weichhart, Thomas Sonstige oth Methods in molecular biology 821 (DE-604)BV035362695 821 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024754776&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | mTor methods and protocols Methods in molecular biology Rapamycin / Laboratory manuals |
| title | mTor methods and protocols |
| title_auth | mTor methods and protocols |
| title_exact_search | mTor methods and protocols |
| title_full | mTor methods and protocols ed. by Thomas Weichhart |
| title_fullStr | mTor methods and protocols ed. by Thomas Weichhart |
| title_full_unstemmed | mTor methods and protocols ed. by Thomas Weichhart |
| title_short | mTor |
| title_sort | mtor methods and protocols |
| title_sub | methods and protocols |
| topic | Rapamycin / Laboratory manuals |
| topic_facet | Rapamycin / Laboratory manuals |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024754776&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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