Bone research protocols:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York, NY [u.a.]
Humana Press, Springer
2012
|
| Ausgabe: | 2. ed |
| Schriftenreihe: | Methods in molecular biology
816 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Klappentext |
| Beschreibung: | Includes bibliographical references and index Primary human osteoblast cultures / Jane P. Dillon ... [et al.] -- Osteoblast isolation from murine calvaria and long bones / Astrid D. Bakker and Jenneke Klein-Nulend -- Rat osteoblast cultures / Isabel R. Orriss, Sarah E.B. Taylor, and Timothy R. Arnett -- Isolation of primary avian osteocytes / Cor M. Semeins, Astrid D. Bakker, and Jenneke Klein-Nulend -- Isolation of mouse osteocytes using cell fractionation for gene expression analysis/ Christine Halleux ... [et al.] -- Studying osteocyte function using the cell lines MLO-Y4 and MLO-A5 / Jennifer Rosser and Lynda F. Bonewald -- Isolation, differentiation, and characterisation of skeletal stem cells from human bone marrow in vitro and in vivo / Rahul S. Tare ... [et al.] -- Rodent osteoclast cultures / Isabel R. Orriss and Timothy R. Arnett -- Isolation and culture of primary chicken osteoclasts / Patricia Collin-Osdoby and Philip Osdoby -- Isolation and purification of rabbit osteoclasts / Fraser P. Coxon, Michael J. Rogers, and Julie C. Crockett -- Generation of human osteoclasts from peripheral blood / Kim Henriksen ... [et al.] -- Osteoclast formation in mouse co-cultures / Cecile Itzstein and Robert J. Van 't Hof -- RANKL-mediated osteoclast formation from murine RAW 264.7 cells / Patricia Collin-Osdoby and Philip Osdoby -- Transfection of osteoclasts and osteoclast precursors / Julie C. Crockett, David J. Mellis, and Adam Taylor -- Analysis of signalling pathways by western blotting and immunoprecipitation / Aymen I. Idris -- Analysis of transcriptional regulation in bone cells / Huilin Jin and Stuart H. Ralston -- Extraction of nucleic acids from bone / Alun Hughes, Tracy L. Stewart, and Val Mann -- Analysis of gene expression in bone by quantitative RT/PCR / Alun Hughes -- Histomorphometry in rodents / Reinhold G. Erben and Martin Glösmann -- Studying gene expression in bone by in situ hybridization / Ina Kramer ... [et al.] -- Immunostaining of skeletal tissues / Tobias B. Kurth and Cosimo De Bari -- Techniques for the study of apoptosis in bone / Sudeh Riahi and Brendon Noble -- Transmission electron microscopy of bone / Vincent Everts ... [et al.] -- Scanning electron microscopy of bone / Alan Boyde -- Fluorescence imaging of osteoclasts using confocal microscopy / Fraser P. Coxon -- Live imaging of bone cell and organ cultures / Sarah L. Dallas and Patricia A. Veno -- Analysis of bone architecture in rodents using microcomputed tomography / Robert J. van 't Hof -- Bone measurements by peripheral quantitative computed tomography in rodents / Jürg A. Gasser and Johannes Willnecker -- Quantitative X-ray imaging of rodent bone by Faxitron / J.H. Duncan Bassett ... [et al.] -- Bioluminescence imaging of bone metastasis in rodents / Thomas J.A. Snoeks ... [et al.] -- Fourier transform infrared imaging of bone / Eleftherios P. Paschalis -- Raman microscopy of bone / Simon R. Goodyear and Richard M. Aspden -- The calvarial injection assay / Robert J. van 't Hof -- Ovariectomy/orchidectomy in rodents / Aymen I. Idris -- Mechanical properties of bone ex vivo / Simon R. Goodyear and Richard M. Aspden -- Mechanical stimulation of bone cells using fluid flow / Carmen Huesa and Astrid D. Bakker -- Using cell and organ culture models to analyze responses of bone cells to mechanical stimulation / Andrew A. Pitsillides and Simon C.F. Rawlinson -- In vivo mechanical loading / Roberto Lopes de Souza and Leanne Saxon |
| Beschreibung: | XIV, 643 S. Ill., graph. Darst. 27 cm |
| ISBN: | 1617794147 9781617794148 |
Internformat
MARC
| LEADER | 00000nam a2200000 cb4500 | ||
|---|---|---|---|
| 001 | BV039895790 | ||
| 003 | DE-604 | ||
| 005 | 20120323 | ||
| 007 | t | ||
| 008 | 120217s2012 ad|| |||| 00||| eng d | ||
| 020 | |a 1617794147 |c (alk. paper) |9 1-61779-414-7 | ||
| 020 | |a 9781617794148 |c (alk. paper) |9 978-1-61779-414-8 | ||
| 035 | |a (OCoLC)756747765 | ||
| 035 | |a (DE-599)BVBBV039895790 | ||
| 040 | |a DE-604 |b ger |e rakwb | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-12 |a DE-355 | ||
| 084 | |a WW 5540 |0 (DE-625)152105:13423 |2 rvk | ||
| 245 | 1 | 0 | |a Bone research protocols |c ed. by Miep H. Helfrich ... |
| 250 | |a 2. ed | ||
| 264 | 1 | |a New York, NY [u.a.] |b Humana Press, Springer |c 2012 | |
| 300 | |a XIV, 643 S. |b Ill., graph. Darst. |c 27 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 816 | |
| 490 | 0 | |a Springer protocols | |
| 500 | |a Includes bibliographical references and index | ||
| 500 | |a Primary human osteoblast cultures / Jane P. Dillon ... [et al.] -- Osteoblast isolation from murine calvaria and long bones / Astrid D. Bakker and Jenneke Klein-Nulend -- Rat osteoblast cultures / Isabel R. Orriss, Sarah E.B. Taylor, and Timothy R. Arnett -- Isolation of primary avian osteocytes / Cor M. Semeins, Astrid D. Bakker, and Jenneke Klein-Nulend -- Isolation of mouse osteocytes using cell fractionation for gene expression analysis/ Christine Halleux ... [et al.] -- Studying osteocyte function using the cell lines MLO-Y4 and MLO-A5 / Jennifer Rosser and Lynda F. Bonewald -- Isolation, differentiation, and characterisation of skeletal stem cells from human bone marrow in vitro and in vivo / Rahul S. Tare ... [et al.] -- Rodent osteoclast cultures / Isabel R. Orriss and Timothy R. Arnett -- Isolation and culture of primary chicken osteoclasts / Patricia Collin-Osdoby and Philip Osdoby -- | ||
| 500 | |a Isolation and purification of rabbit osteoclasts / Fraser P. Coxon, Michael J. Rogers, and Julie C. Crockett -- Generation of human osteoclasts from peripheral blood / Kim Henriksen ... [et al.] -- Osteoclast formation in mouse co-cultures / Cecile Itzstein and Robert J. Van 't Hof -- RANKL-mediated osteoclast formation from murine RAW 264.7 cells / Patricia Collin-Osdoby and Philip Osdoby -- Transfection of osteoclasts and osteoclast precursors / Julie C. Crockett, David J. Mellis, and Adam Taylor -- Analysis of signalling pathways by western blotting and immunoprecipitation / Aymen I. Idris -- Analysis of transcriptional regulation in bone cells / Huilin Jin and Stuart H. Ralston -- Extraction of nucleic acids from bone / Alun Hughes, Tracy L. Stewart, and Val Mann -- Analysis of gene expression in bone by quantitative RT/PCR / Alun Hughes -- Histomorphometry in rodents / Reinhold G. Erben and Martin Glösmann -- | ||
| 500 | |a Studying gene expression in bone by in situ hybridization / Ina Kramer ... [et al.] -- Immunostaining of skeletal tissues / Tobias B. Kurth and Cosimo De Bari -- Techniques for the study of apoptosis in bone / Sudeh Riahi and Brendon Noble -- Transmission electron microscopy of bone / Vincent Everts ... [et al.] -- Scanning electron microscopy of bone / Alan Boyde -- Fluorescence imaging of osteoclasts using confocal microscopy / Fraser P. Coxon -- Live imaging of bone cell and organ cultures / Sarah L. Dallas and Patricia A. Veno -- Analysis of bone architecture in rodents using microcomputed tomography / Robert J. van 't Hof -- Bone measurements by peripheral quantitative computed tomography in rodents / Jürg A. Gasser and Johannes Willnecker -- Quantitative X-ray imaging of rodent bone by Faxitron / J.H. Duncan Bassett ... [et al.] -- Bioluminescence imaging of bone metastasis in rodents / Thomas J.A. Snoeks ... [et al.] -- | ||
| 500 | |a Fourier transform infrared imaging of bone / Eleftherios P. Paschalis -- Raman microscopy of bone / Simon R. Goodyear and Richard M. Aspden -- The calvarial injection assay / Robert J. van 't Hof -- Ovariectomy/orchidectomy in rodents / Aymen I. Idris -- Mechanical properties of bone ex vivo / Simon R. Goodyear and Richard M. Aspden -- Mechanical stimulation of bone cells using fluid flow / Carmen Huesa and Astrid D. Bakker -- Using cell and organ culture models to analyze responses of bone cells to mechanical stimulation / Andrew A. Pitsillides and Simon C.F. Rawlinson -- In vivo mechanical loading / Roberto Lopes de Souza and Leanne Saxon | ||
| 650 | 4 | |a Bones / Cultures and culture media / Laboratory manuals | |
| 650 | 4 | |a Bone cells / Laboratory manuals | |
| 650 | 4 | |a Bone and Bones / Laboratory Manuals | |
| 650 | 4 | |a Osteoblasts / Laboratory Manuals | |
| 650 | 4 | |a Cytological Techniques / Laboratory Manuals | |
| 650 | 0 | 7 | |a Knochen |0 (DE-588)4128031-3 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Molekulare Medizin |0 (DE-588)4543844-4 |2 gnd |9 rswk-swf |
| 689 | 0 | 0 | |a Knochen |0 (DE-588)4128031-3 |D s |
| 689 | 0 | 1 | |a Molekulare Medizin |0 (DE-588)4543844-4 |D s |
| 689 | 0 | |5 DE-604 | |
| 700 | 1 | |a Helfrich, Miep H. |e Sonstige |4 oth | |
| 776 | 0 | 8 | |i Erscheint auch als |n Online-Ausgabe |z 1-61779-415-5 |
| 776 | 0 | 8 | |i Erscheint auch als |n Online-Ausgabe |z 978-1-61779-415-5 |
| 830 | 0 | |a Methods in molecular biology |v 816 |w (DE-604)BV035362695 |9 816 | |
| 856 | 4 | 2 | |m Digitalisierung UB Regensburg |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024754745&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 856 | 4 | 2 | |m Digitalisierung UB Regensburg |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024754745&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA |3 Klappentext |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-024754745 | ||
Datensatz im Suchindex
| _version_ | 1804148846536687616 |
|---|---|
| adam_text | Contents
Preface
.......................................................... v
Contributors.
..................................................... xi
Part I Culture of Osteoblasts and Osteocytes
1
Primary Human Osteoblast Cultures
................................ 3
Jane P. Dillon, Victoria J. Waring-Green,
Adam M. Taylor, Peter J.M. Wilson, Mark Birch,
Alison Gartland, and James A. Gallagher
2
Osteoblast Isolation from
Murine
Calvaría
and Long Bones
.............. 19
Astrid
D.
Bakker andjenneke Klein-Nulend
3
Rat Osteoblast
Cultures
......................................... 31
Isabel
R.
Orriss, Sarah E.B. Taylor,
and Timothy R. Arnett
4
Isolation of Primary Avian Osteocytes
............................... 43
Cor M.
Semeins, Astrid D.
Bakker,
andjenneke
Klein-Nulend
5
Isolation of Mouse Osteocytes Using Cell Fractionation
for Gene Expression Analysis
...................................... 55
Christine Halleux,
Ina
Kramer, Cyril Allard,
and
Michaela Kneissel
6
Studying Osteocyte Function Using the Cell Lines
MLO-Y4 and MLO-A5
.......................................... 67
Jennifer
Rosser
and Lynda
F. Bonewald
7
Isolation, Differentiation, and Characterisation of Skeletal Stem Cells
from Human Bone Marrow In Vitro and In Vivo
...................... 83
Rahul S. Tare, Peter D. Mitchell,
Janos
Kanczler,
and Richard O.C. Oreffo
Part II Culture of Osteoclasts
8
Rodent Osteodast Cultures
....................................... 103
Isabel R. Orriss and Timothy R. Arnett
9
Isolation and Culture of Primary Chicken Osteoclasts
................... 119
Patricia Collin-Osdoby and Philip Osdoby
10
Isolation and Purification of Rabbit Osteoclasts
........................ 145
Fraser
P. Coxon, Michael J. Rogers, and Julie C. Crockett
VII
viii Contents
11 Generation
of
Human
Osteoclasts from Peripheral Blood
................ 159
Kim Henriben,
Morten A. Karsdal,
Adam Taylor,
Denise
Tosh,
and Fraser
P. Coxon
12
Osteoclast Formation in Mouse Co-cultures
.......................... 177
Cecile
Itzstein and Robert J. van
t Hof
13
RANKL-Mediated Osteoclast Formation from
Murine
RAW
264.7
cells
............................................... 187
Patricia Collin-Osdoby and Philip Osdoby
Part III Biochemical and Molecular Analysis of Bone Cells
14
Transfection of Osteoclasts and Osteoclast Precursors
................... 205
Julie
С
Crockett, David J. Mellis, and Adam Taylor
15
Analysis of Signalling Pathways by Western Blotting
and Immunoprecipitation
........................................ 223
Aymen I. Idris
16
Analysis of Transcriptional Regulation in Bone Cells
.................... 233
Huilinjin and Stuart H. Ralston
17
Extraction of Nucleic Acids from Bone
.............................. 249
Alun
Hughes, Tracy L. Stewart, and
Val Mann
18
Analysis of Gene Expression in Bone by Quantitative RT/PCR
............ 261
Alun
Hughes
Part IV Microscopical Techniques
19
Histomorphometry in Rodents
.................................... 279
Reinhold G.
Erben
and Martin
Glosmann
20
Studying Gene Expression in Bone by In Situ Hybridization
.............. 305
Ina
Kramer, Rishard
Salie,
Mira Susa,
and
Michaela Kneissel
21
Immunostaining of Skeletal Tissues
................................. 321
Tobias B. Kurth and Cosimo
De Bari
22
Techniques for the Study of Apoptosis in Bone
........................ 335
Sudeh Riahi and Brendan Noble
23
Transmission Electron Microscopy of Bone
........................... 351
Vincent Everts, Anneke Niehof, Wikky
Tigchelaar-Gutter, and
Wouter
Beertsen
24
Scanning Electron Microscopy of Bone
.............................. 365
Alan Boyde
25
Fluorescence Imaging of Osteoclasts Using Confocal Microscopy
.......... 401
Fraser
P. Coxon
26
live Imaging of Bone Cell and Organ Cultures
........................ 425
Sarah L· Dallas and Patricia
A. Veno
Contents ix
Part V Imaging Techniques
27
Analysis of Bone Architecture in Rodents Using
Microcomputed Tomography
..................................... 461
Robert J. van tHof
28
Bone Measurements by Peripheral Quantitative Computed
Tomography in Rodents
......................................... 477
JürgA.
Gasser and Johannes Willnecker
29
Quantitative X-ray Imaging of Rodent Bone by Faxitron
................. 499
J.H. Duncan Bassett, Anne van
der Spek,
Apostólos
Gogakos,
and Graham R. Williams
30
Bioluminescence Imaging of Bone Metastasis in Rodents
................ 507
Thomas].A. Snoeks, Ermond van
Beek,
Ivo
Que,
Eric
L.
Kaijzel,
and Clemens
W.G.M. Löwik
31
Fourier Transform Infrared Imaging of Bone
......................... 517
EleftheriosP. Paschalis
32
Raman Microscopy of Bone
...................................... 527
Simon R. Goodyear and Richard M. Aspden
Part VI In vivo Techniques
33
The Calvarial Injection Assay
...................................... 537
Robert J. van tHof
34
Ovariectomy/Orchidectomy in Rodents
............................. 545
Aymen I. Idris
Part
VII
Mechanical Loading Techniques
35
Mechanical Properties of Bone Ex Vivo
.............................. 555
Simon R. Goodyear and
Richard M.
Aspden
36
Mechanical Stimulation of Bone Cells Using Fluid Flow
................. 573
Carmen
Huesa
and
Astrid
D.
Bakker
37
Using Cell and Organ Culture Models to Analyze Responses
of Bone Cells to Mechanical Stimulation
............................. 593
Andrew A. Pitsillides and Simon
CF. Rawlinson
38
In Vivo Mechanical Loading
...................................... 621
Roberto Lopes
de Souza
and Leanne Saxon
Index
........................................................... 637
Methods in Molecular Biology
816
John M. Walker, Series Editor
Miep H. Helfrich
·
Stuart H. Ralston Editors
Bone Research Protocols
Second Edition
Studies over the past decade have continued to bring tremendous advances to our understanding
ofbone biology. New pathways have been discovered and expanded our knowledge of the ways
in which genes and gene products affect bone cells and thereby bone mass and bone strength.
In Bone Research Protocols, Second Edition, expert researchers in the field detail many methods
commonly used to study bone biology. Focusing mainly on in vitro methods, this volume gives
techniques for isolation, culture and functional analysis of all bone cell types and details a range
of imaging methods, including light and
ultrastructural
microscopy and live cell imaging. Some
important in vivo techniques are included, such as analysis ofbone
résorption
and imaging using
X rays, fluorescent or luminescent techniques. Methods for study of proteins and nucleic acid
are included and methods for analysis ofbone composition, measurement ofbone strength, and
response to mechanical stimulation are described. Written in the highly successful Methods in
Molecular Biology series format, chapters include introductions to their respective topics, lists
of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols,
and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Bone Research Protocols, Second Edition seeks to aid scientists in
the bone field to establish new techniques in their laboratories.
|
| any_adam_object | 1 |
| building | Verbundindex |
| bvnumber | BV039895790 |
| classification_rvk | WW 5540 |
| ctrlnum | (OCoLC)756747765 (DE-599)BVBBV039895790 |
| discipline | Biologie |
| edition | 2. ed |
| format | Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>05791nam a2200553 cb4500</leader><controlfield tag="001">BV039895790</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20120323 </controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">120217s2012 ad|| |||| 00||| eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">1617794147</subfield><subfield code="c">(alk. paper)</subfield><subfield code="9">1-61779-414-7</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9781617794148</subfield><subfield code="c">(alk. paper)</subfield><subfield code="9">978-1-61779-414-8</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)756747765</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV039895790</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-12</subfield><subfield code="a">DE-355</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WW 5540</subfield><subfield code="0">(DE-625)152105:13423</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Bone research protocols</subfield><subfield code="c">ed. by Miep H. Helfrich ...</subfield></datafield><datafield tag="250" ind1=" " ind2=" "><subfield code="a">2. ed</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">New York, NY [u.a.]</subfield><subfield code="b">Humana Press, Springer</subfield><subfield code="c">2012</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">XIV, 643 S.</subfield><subfield code="b">Ill., graph. Darst.</subfield><subfield code="c">27 cm</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="1" ind2=" "><subfield code="a">Methods in molecular biology</subfield><subfield code="v">816</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">Springer protocols</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">Includes bibliographical references and index</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">Primary human osteoblast cultures / Jane P. Dillon ... [et al.] -- Osteoblast isolation from murine calvaria and long bones / Astrid D. Bakker and Jenneke Klein-Nulend -- Rat osteoblast cultures / Isabel R. Orriss, Sarah E.B. Taylor, and Timothy R. Arnett -- Isolation of primary avian osteocytes / Cor M. Semeins, Astrid D. Bakker, and Jenneke Klein-Nulend -- Isolation of mouse osteocytes using cell fractionation for gene expression analysis/ Christine Halleux ... [et al.] -- Studying osteocyte function using the cell lines MLO-Y4 and MLO-A5 / Jennifer Rosser and Lynda F. Bonewald -- Isolation, differentiation, and characterisation of skeletal stem cells from human bone marrow in vitro and in vivo / Rahul S. Tare ... [et al.] -- Rodent osteoclast cultures / Isabel R. Orriss and Timothy R. Arnett -- Isolation and culture of primary chicken osteoclasts / Patricia Collin-Osdoby and Philip Osdoby -- </subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">Isolation and purification of rabbit osteoclasts / Fraser P. Coxon, Michael J. Rogers, and Julie C. Crockett -- Generation of human osteoclasts from peripheral blood / Kim Henriksen ... [et al.] -- Osteoclast formation in mouse co-cultures / Cecile Itzstein and Robert J. Van 't Hof -- RANKL-mediated osteoclast formation from murine RAW 264.7 cells / Patricia Collin-Osdoby and Philip Osdoby -- Transfection of osteoclasts and osteoclast precursors / Julie C. Crockett, David J. Mellis, and Adam Taylor -- Analysis of signalling pathways by western blotting and immunoprecipitation / Aymen I. Idris -- Analysis of transcriptional regulation in bone cells / Huilin Jin and Stuart H. Ralston -- Extraction of nucleic acids from bone / Alun Hughes, Tracy L. Stewart, and Val Mann -- Analysis of gene expression in bone by quantitative RT/PCR / Alun Hughes -- Histomorphometry in rodents / Reinhold G. Erben and Martin Glösmann -- </subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">Studying gene expression in bone by in situ hybridization / Ina Kramer ... [et al.] -- Immunostaining of skeletal tissues / Tobias B. Kurth and Cosimo De Bari -- Techniques for the study of apoptosis in bone / Sudeh Riahi and Brendon Noble -- Transmission electron microscopy of bone / Vincent Everts ... [et al.] -- Scanning electron microscopy of bone / Alan Boyde -- Fluorescence imaging of osteoclasts using confocal microscopy / Fraser P. Coxon -- Live imaging of bone cell and organ cultures / Sarah L. Dallas and Patricia A. Veno -- Analysis of bone architecture in rodents using microcomputed tomography / Robert J. van 't Hof -- Bone measurements by peripheral quantitative computed tomography in rodents / Jürg A. Gasser and Johannes Willnecker -- Quantitative X-ray imaging of rodent bone by Faxitron / J.H. Duncan Bassett ... [et al.] -- Bioluminescence imaging of bone metastasis in rodents / Thomas J.A. Snoeks ... [et al.] -- </subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">Fourier transform infrared imaging of bone / Eleftherios P. Paschalis -- Raman microscopy of bone / Simon R. Goodyear and Richard M. Aspden -- The calvarial injection assay / Robert J. van 't Hof -- Ovariectomy/orchidectomy in rodents / Aymen I. Idris -- Mechanical properties of bone ex vivo / Simon R. Goodyear and Richard M. Aspden -- Mechanical stimulation of bone cells using fluid flow / Carmen Huesa and Astrid D. Bakker -- Using cell and organ culture models to analyze responses of bone cells to mechanical stimulation / Andrew A. Pitsillides and Simon C.F. Rawlinson -- In vivo mechanical loading / Roberto Lopes de Souza and Leanne Saxon</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Bones / Cultures and culture media / Laboratory manuals</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Bone cells / Laboratory manuals</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Bone and Bones / Laboratory Manuals</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Osteoblasts / Laboratory Manuals</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cytological Techniques / Laboratory Manuals</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Knochen</subfield><subfield code="0">(DE-588)4128031-3</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Molekulare Medizin</subfield><subfield code="0">(DE-588)4543844-4</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Knochen</subfield><subfield code="0">(DE-588)4128031-3</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Molekulare Medizin</subfield><subfield code="0">(DE-588)4543844-4</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Helfrich, Miep H.</subfield><subfield code="e">Sonstige</subfield><subfield code="4">oth</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Online-Ausgabe</subfield><subfield code="z">1-61779-415-5</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Online-Ausgabe</subfield><subfield code="z">978-1-61779-415-5</subfield></datafield><datafield tag="830" ind1=" " ind2="0"><subfield code="a">Methods in molecular biology</subfield><subfield code="v">816</subfield><subfield code="w">(DE-604)BV035362695</subfield><subfield code="9">816</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">Digitalisierung UB Regensburg</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024754745&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">Digitalisierung UB Regensburg</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024754745&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Klappentext</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-024754745</subfield></datafield></record></collection> |
| id | DE-604.BV039895790 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T00:13:37Z |
| institution | BVB |
| isbn | 1617794147 9781617794148 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-024754745 |
| oclc_num | 756747765 |
| open_access_boolean | |
| owner | DE-12 DE-355 DE-BY-UBR |
| owner_facet | DE-12 DE-355 DE-BY-UBR |
| physical | XIV, 643 S. Ill., graph. Darst. 27 cm |
| publishDate | 2012 |
| publishDateSearch | 2012 |
| publishDateSort | 2012 |
| publisher | Humana Press, Springer |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | Bone research protocols ed. by Miep H. Helfrich ... 2. ed New York, NY [u.a.] Humana Press, Springer 2012 XIV, 643 S. Ill., graph. Darst. 27 cm txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 816 Springer protocols Includes bibliographical references and index Primary human osteoblast cultures / Jane P. Dillon ... [et al.] -- Osteoblast isolation from murine calvaria and long bones / Astrid D. Bakker and Jenneke Klein-Nulend -- Rat osteoblast cultures / Isabel R. Orriss, Sarah E.B. Taylor, and Timothy R. Arnett -- Isolation of primary avian osteocytes / Cor M. Semeins, Astrid D. Bakker, and Jenneke Klein-Nulend -- Isolation of mouse osteocytes using cell fractionation for gene expression analysis/ Christine Halleux ... [et al.] -- Studying osteocyte function using the cell lines MLO-Y4 and MLO-A5 / Jennifer Rosser and Lynda F. Bonewald -- Isolation, differentiation, and characterisation of skeletal stem cells from human bone marrow in vitro and in vivo / Rahul S. Tare ... [et al.] -- Rodent osteoclast cultures / Isabel R. Orriss and Timothy R. Arnett -- Isolation and culture of primary chicken osteoclasts / Patricia Collin-Osdoby and Philip Osdoby -- Isolation and purification of rabbit osteoclasts / Fraser P. Coxon, Michael J. Rogers, and Julie C. Crockett -- Generation of human osteoclasts from peripheral blood / Kim Henriksen ... [et al.] -- Osteoclast formation in mouse co-cultures / Cecile Itzstein and Robert J. Van 't Hof -- RANKL-mediated osteoclast formation from murine RAW 264.7 cells / Patricia Collin-Osdoby and Philip Osdoby -- Transfection of osteoclasts and osteoclast precursors / Julie C. Crockett, David J. Mellis, and Adam Taylor -- Analysis of signalling pathways by western blotting and immunoprecipitation / Aymen I. Idris -- Analysis of transcriptional regulation in bone cells / Huilin Jin and Stuart H. Ralston -- Extraction of nucleic acids from bone / Alun Hughes, Tracy L. Stewart, and Val Mann -- Analysis of gene expression in bone by quantitative RT/PCR / Alun Hughes -- Histomorphometry in rodents / Reinhold G. Erben and Martin Glösmann -- Studying gene expression in bone by in situ hybridization / Ina Kramer ... [et al.] -- Immunostaining of skeletal tissues / Tobias B. Kurth and Cosimo De Bari -- Techniques for the study of apoptosis in bone / Sudeh Riahi and Brendon Noble -- Transmission electron microscopy of bone / Vincent Everts ... [et al.] -- Scanning electron microscopy of bone / Alan Boyde -- Fluorescence imaging of osteoclasts using confocal microscopy / Fraser P. Coxon -- Live imaging of bone cell and organ cultures / Sarah L. Dallas and Patricia A. Veno -- Analysis of bone architecture in rodents using microcomputed tomography / Robert J. van 't Hof -- Bone measurements by peripheral quantitative computed tomography in rodents / Jürg A. Gasser and Johannes Willnecker -- Quantitative X-ray imaging of rodent bone by Faxitron / J.H. Duncan Bassett ... [et al.] -- Bioluminescence imaging of bone metastasis in rodents / Thomas J.A. Snoeks ... [et al.] -- Fourier transform infrared imaging of bone / Eleftherios P. Paschalis -- Raman microscopy of bone / Simon R. Goodyear and Richard M. Aspden -- The calvarial injection assay / Robert J. van 't Hof -- Ovariectomy/orchidectomy in rodents / Aymen I. Idris -- Mechanical properties of bone ex vivo / Simon R. Goodyear and Richard M. Aspden -- Mechanical stimulation of bone cells using fluid flow / Carmen Huesa and Astrid D. Bakker -- Using cell and organ culture models to analyze responses of bone cells to mechanical stimulation / Andrew A. Pitsillides and Simon C.F. Rawlinson -- In vivo mechanical loading / Roberto Lopes de Souza and Leanne Saxon Bones / Cultures and culture media / Laboratory manuals Bone cells / Laboratory manuals Bone and Bones / Laboratory Manuals Osteoblasts / Laboratory Manuals Cytological Techniques / Laboratory Manuals Knochen (DE-588)4128031-3 gnd rswk-swf Molekulare Medizin (DE-588)4543844-4 gnd rswk-swf Knochen (DE-588)4128031-3 s Molekulare Medizin (DE-588)4543844-4 s DE-604 Helfrich, Miep H. Sonstige oth Erscheint auch als Online-Ausgabe 1-61779-415-5 Erscheint auch als Online-Ausgabe 978-1-61779-415-5 Methods in molecular biology 816 (DE-604)BV035362695 816 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024754745&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024754745&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA Klappentext |
| spellingShingle | Bone research protocols Methods in molecular biology Bones / Cultures and culture media / Laboratory manuals Bone cells / Laboratory manuals Bone and Bones / Laboratory Manuals Osteoblasts / Laboratory Manuals Cytological Techniques / Laboratory Manuals Knochen (DE-588)4128031-3 gnd Molekulare Medizin (DE-588)4543844-4 gnd |
| subject_GND | (DE-588)4128031-3 (DE-588)4543844-4 |
| title | Bone research protocols |
| title_auth | Bone research protocols |
| title_exact_search | Bone research protocols |
| title_full | Bone research protocols ed. by Miep H. Helfrich ... |
| title_fullStr | Bone research protocols ed. by Miep H. Helfrich ... |
| title_full_unstemmed | Bone research protocols ed. by Miep H. Helfrich ... |
| title_short | Bone research protocols |
| title_sort | bone research protocols |
| topic | Bones / Cultures and culture media / Laboratory manuals Bone cells / Laboratory manuals Bone and Bones / Laboratory Manuals Osteoblasts / Laboratory Manuals Cytological Techniques / Laboratory Manuals Knochen (DE-588)4128031-3 gnd Molekulare Medizin (DE-588)4543844-4 gnd |
| topic_facet | Bones / Cultures and culture media / Laboratory manuals Bone cells / Laboratory manuals Bone and Bones / Laboratory Manuals Osteoblasts / Laboratory Manuals Cytological Techniques / Laboratory Manuals Knochen Molekulare Medizin |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024754745&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024754745&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035362695 |
| work_keys_str_mv | AT helfrichmieph boneresearchprotocols |