The enzymatic synthesis of prebiotic lactulose in dairy matrices: set-up, analysis, process implementation and health effects
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Düsseldorf
VDI-Verl.
2011
|
| Ausgabe: | Als Ms. gedr. |
| Schriftenreihe: | Fortschritt-Berichte VDI
Reihe 14, Landtechnik, Lebensmitteltechnik ; 138 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XVIII, 182 S. graph. Darst. 21 cm |
| ISBN: | 9783183138142 |
Internformat
MARC
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| 245 | 1 | 0 | |a The enzymatic synthesis of prebiotic lactulose in dairy matrices |b set-up, analysis, process implementation and health effects |c Regina Lucie Schuster-Wolff-Bühring |
| 250 | |a Als Ms. gedr. | ||
| 264 | 1 | |a Düsseldorf |b VDI-Verl. |c 2011 | |
| 300 | |a XVIII, 182 S. |b graph. Darst. |c 21 cm | ||
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| 490 | 1 | |a Fortschritt-Berichte VDI : Reihe 14, Landtechnik, Lebensmitteltechnik |v 138 | |
| 502 | |a Zugl.: Hohenheim, Univ., Diss., 2011 | ||
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IMAGE 1
TABLE O F CONTENTS
SYMBOLS AND ABBREVIATIONS X
SUMMARY XV
ZUSAMMENFASSUNG XVII
1 INTRODUCTION 1
2 PRODUCTION AND PHYSIOLOGICAL ACTION O F THE DISACCHARIDE LACTULOSE 5
2.1 INTRODUCTION 6
2.2 LACTULOSE FORMATION IN MILK DURING HEAT TREATMENT 7
2.3 LACTULOSE PRODUCTION 8
2.3.1 ISOMERIZATION OF LACTOSE 9
2.3.1.1 ALKALISING CATALYSTS 9
2.3.1.2 COMPLEXING AGENTS 10
2.3.1.3 ION-MEDIATED PROCESSES 11
2.3.1.4 DOWNSTREAM PROCESSING OF THE LA-TRANSFORMATION 12
2.3.2 ENZYMATIC CATALYSIS OF LACTULOSE FORMATION 14
2.3.2.1 REDOX ISOMERIZATION OF LACTOSE 15
2.3.2.2 LACTULOSE FORMATION BY TRANSGALACTOSYLATION 16
2.4. PHYSIOLOGICAL EFFECTS OF LACTULOSE 19
2.4.1 PRIMARY EFFECTS OF LACTULOSE INGESTION 19
2.4.2 LACTULOSE IN THE TREATMENT OF CONSTIPATION 20
2.4.3 LACTULOSE IN THE TREATMENT OF HEPATIC ENCEPHALOPATHY 21
2.4.4 PREBIOTIC ACTION 22
2.4.5 ENHANCEMENT OF MINERAL ABSORPTION 23
2.4.6 LACTULOSE AND COLON CARCINOGENESIS 24
2.4.7 DIAGNOSTIC APPLICATION OF LACTULOSE 2 4
2.5 SAFETY AND REGULATORY STATUS OF LACTULOSE 25
2.6 OUTLOOK: LACTULOSE AS FOOD INGREDIENT 26
2.7 REFERENCES 30
3 ENZYMATIC SYNTHESIS O F THE PREBIOTIC SUGAR
LACTULOSE IN SKIM MILK ULTRAFILTRATION PERMEATE 38
HTTP://D-NB.INFO/1019702672
IMAGE 2
3.1 INTRODUCTION 39
3.2 MATERIALS & METHODS 4 0
3.3 RESULTS 4 3
3.3.1 GENERAL REACTION COURSE 4 3
3.3.2 FRUCTOSE CONCENTRATION 4 4
3.3.3 TEMPERATURE AND ENZYME ACTIVITY 45
3.3.4 MICROBIAL SOURCE OF /3-GALACTOSIDASE 4 6
3.4 DISCUSSION 4 9
3.4.1 GENERAL REACTION COURSE 4 9
3.4.2 FRUCTOSE CONCENTRATION 4 9
3.4.3 TEMPERATURE AND ENZYME ACTIVITY 51
3.4.4 MICROBIAL SOURCE OF/3-GALACTOSIDASE 51
3.5 CONCLUSION 52
3.6 REFERENCES 54
4 A NEW LIQUID CHROMATOGRAPHY METHOD F O R THE
SIMULTANEOUS AND SENSITIVE QUANTIFICATION OF LACTOSE AND LACTULOSE IN
MILK 57
4.1 INTRODUCTION 58
4.2 MATERIALS AND METHODS 59
4.3 RESULTS 6 2
4.3.1 COMPARISON OF STATIONARY PHASES 62
4.3.2 METHOD EVALUATION 66
4.3.3 APPLICATION TO REAL SAMPLES 68
4.4 DISCUSSION 69
4.4.1 COMPARISON OF STATIONARY PHASES 69
4.4.2 METHOD DEVELOPMENT 70
4.5 CONCLUSION 70
4.6 REFERENCES 71
5 INACTIVATION O F /?-GALACTOSIDASES FROM A. ORYZAE
AND E. COLI 74
5.A THERMAL INACTIVATION OF /?-GALACTOSIDASES FROM A. ORYZAE AND E.
COLI: KINETICS AND PROCESS DESIGN 75
5.A.1 INTRODUCTION 76
5.A.2 MATERIALS & METHODS 78
5.A.3 RESULTS 81
5.A.3.1 INACTIVATION KINETICS OF /3-GALACTOSIDASE FROM A. ORYZAE 81
5.A.3.2 INACTIVATION KINETICS OF /3-GALACTOSIDASE FROM E. COLI 84
5.A.4 DISCUSSION 85
5.A.5 CONCLUSION 86
IMAGE 3
5.B KINETICS OF THE PRESSURE-DRIVEN INACTIVATION OF A FUNGAL /?-
GALACTOSIDASE: APPLICATION OF A SECOND-ORDER PHASE-TRANSITION MODEL 88
5.B.1 INTRODUCTION 89
5.B.2 MATERIALS & METHODS 9 0
5.B.3 RESULTS 93
5.B.3.1 REACTION RATES OF HYDROSTATIC-THERMAL INACTIVATION OF/3-
GALACTOSIDASE 93
5.B.3.2 THERMODYNAMIC ASPECTS OF HYDROSTATIC-THERMAL TREATMENT OF THE
/3-GALACTOSIDASE FROM A. ORYZAE 97
5.B.4 DISCUSSION 99
5.B.4.1 REACTION RATES OF HYDROSTATIC-THERMAL INACTIVATION OF /3-
GALACTOSIDASE 99
5.B.4.2 THERMODYNAMIC ASPECTS OF HYDROSTATIC-THERMAL TREATMENT OF THE
/3-GALACTOSIDASE FROM A. ORYZAE 100
5.B.5 CONCLUSION 101
5.C REFERENCES 103
6 SYNTHESIS O F LACTULOSE B Y TRANSGALACTOSYLATION OF
FRUCTOSE: STRUCTURAL ASSIGNMENT O F REACTION PRODUCTS VIA NMR 106
6.1 INTRODUCTION 107
6.2 MATERIALS & METHODS 108
6.3 RESULTS 112
6.3.1 REACTION PRODUCTS OF ENZYMATIC TRANSGALACTOSYLATION OF FRUCTOSE
112
6.3.2 NMR-ASSIGNMENT OF REACTION PRODUCTS 113
6.3.3 BIOTRANSFORMATION WITH /3-GALACTOSIDASES FROM A. ORYZAE AND E . C
O L I 114
6.4 DISCUSSION 118
6.4.1 REACTION PRODUCTS OF ENZYMATIC TRANSGALACTOSYLATION OF FRUCTOSE
118
6.4.2 NMR-ASSIGNMENT OF REACTION PRODUCTS 118
6.4.3 BIOTRANSFORMATION WITH /3-GALACTOSIDASES FROM A. ORYZAE AND E . C
O L I 120
6.5 CONCLUSION T 122
6.6 REFERENCES 124
7 MODELLING THE ENZYMATIC FORMATION O F LACTULOSE 127 \
7.A EMPIRICAL MODELLING OF THE ENZYMATIC SYNTHESIS OF LACTULOSE 128
IMAGE 4
7.A.1 INTRODUCTION 129
7.A.2 MATERIALS & METHODS 130
7.A.3 RESULTS: MODEL DESIGN 131
7.A.3.1 LACTULOSE FORMATION 131
7.A.3.2 LACTULOSE HYDROLYSIS 135
7.A.4 DISCUSSION 139
7.A.5 CONCLUSION 140
7.B MODELLING OF THE TRANSGALACTOSYLATION REACTION ACCORDING TO K I N G
& A L T M A N 141
7.B.1 INTRODUCTION 142
7.B.2 MATERIALS & METHODS 143
7.B.3 RESULTS 144
7.B.3.1 MODEL DEVELOPMENT 144
7.B.3.2 BIOTRANSFORMATION WITH A /3-GALACTOSIDASE FROM E. COLI 146
7.B.3.3 BIOTRANSFORMATION WITH A /3-GALACTOSIDASE FROM A. ORYZAE 147
7.B.3.4 BIOTRANSFORMATION WITH A /3-GALACTOSIDASE FROM K. LACTIS 148
7.B.4 DISCUSSION 149
7.B.5 CONCLUSION 151
7.C REFERENCES 155
8 ENZYMATIC SYNTHESIS O F PREBIOTIC LACTULOSE IN DAIRY
PRODUCTS: A HUMAN CLINICAL STUDY ON ITS FUNCTIONAL EFFECTS 156
8.1 INTRODUCTION 157
8.2 MATERIALS & METHODS 158
8.3 RESULTS 163
8.3.1 REALIZATION OF ENZYMATIC LACTULOSE SYNTHESIS IN TECHNICAL SCALE
163
8.3.2 HEALTH STATE OF TEST PERSONS 165
8.3.3 ANALYSIS OF STOOL SAMPLES 166
8.3.4 MICROBIAL ANALYSIS OF FAECES 167
8.4 DISCUSSION , 169
8.4.1 REALIZATION OF ENZYMATIC LACTULOSE SYNTHESIS IN TECHNICAL SCALE
169
8.4.2 CLINICAL STUDY ON THE PHYSIOLOGICAL EFFECTS OF LACTULOSE 170
8.5 CONCLUSION 173
8.6 REFERENCES 174
9 OUTLOOK 177
9.1 DOWNSTREAM PROCESSING 177
9.2 BRINGING THE TRANSGALACTOSYLATION REACTION FORWARD TO NEW PRODUCTS
178
9.3 MICROBIAL TRANSGALACTOSYLATION 179
9.4 REFERENCES 181
IMAGE 5
COAUTHORS |
| any_adam_object | 1 |
| author | Schuster-Wolff-Bühring, Regina |
| author_GND | (DE-588)1016387628 |
| author_facet | Schuster-Wolff-Bühring, Regina |
| author_role | aut |
| author_sort | Schuster-Wolff-Bühring, Regina |
| author_variant | r s w rsw |
| building | Verbundindex |
| bvnumber | BV039881045 |
| classification_rvk | VN 8950 VN 8957 |
| ctrlnum | (OCoLC)780115097 (DE-599)BSZ358302714 |
| dewey-full | 637 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 637 - Processing dairy and related products |
| dewey-raw | 637 |
| dewey-search | 637 |
| dewey-sort | 3637 |
| dewey-tens | 630 - Agriculture and related technologies |
| discipline | Chemie / Pharmazie Agrar-/Forst-/Ernährungs-/Haushaltswissenschaft / Gartenbau |
| edition | Als Ms. gedr. |
| format | Thesis Book |
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| id | DE-604.BV039881045 |
| illustrated | Illustrated |
| indexdate | 2024-10-29T15:02:03Z |
| institution | BVB |
| isbn | 9783183138142 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-024740279 |
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| physical | XVIII, 182 S. graph. Darst. 21 cm |
| publishDate | 2011 |
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| series | Fortschritt-Berichte VDI |
| series2 | Fortschritt-Berichte VDI : Reihe 14, Landtechnik, Lebensmitteltechnik |
| spelling | Schuster-Wolff-Bühring, Regina Verfasser (DE-588)1016387628 aut The enzymatic synthesis of prebiotic lactulose in dairy matrices set-up, analysis, process implementation and health effects Regina Lucie Schuster-Wolff-Bühring Als Ms. gedr. Düsseldorf VDI-Verl. 2011 XVIII, 182 S. graph. Darst. 21 cm txt rdacontent n rdamedia nc rdacarrier Fortschritt-Berichte VDI : Reihe 14, Landtechnik, Lebensmitteltechnik 138 Zugl.: Hohenheim, Univ., Diss., 2011 Fructose (DE-588)4155521-1 gnd rswk-swf Milchprodukt (DE-588)4235506-0 gnd rswk-swf Zusatz (DE-588)4132717-2 gnd rswk-swf Enzymkatalyse (DE-588)4152480-9 gnd rswk-swf Galactosidase beta- (DE-588)4336804-9 gnd rswk-swf Molke (DE-588)4140861-5 gnd rswk-swf Ballaststoff (DE-588)4004346-0 gnd rswk-swf Hydrolyse (DE-588)4160918-9 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Molke (DE-588)4140861-5 s Fructose (DE-588)4155521-1 s Zusatz (DE-588)4132717-2 s Hydrolyse (DE-588)4160918-9 s Enzymkatalyse (DE-588)4152480-9 s Galactosidase beta- (DE-588)4336804-9 s Milchprodukt (DE-588)4235506-0 s Ballaststoff (DE-588)4004346-0 s DE-604 Fortschritt-Berichte VDI Reihe 14, Landtechnik, Lebensmitteltechnik ; 138 (DE-604)BV000021889 138 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024740279&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Schuster-Wolff-Bühring, Regina The enzymatic synthesis of prebiotic lactulose in dairy matrices set-up, analysis, process implementation and health effects Fortschritt-Berichte VDI Fructose (DE-588)4155521-1 gnd Milchprodukt (DE-588)4235506-0 gnd Zusatz (DE-588)4132717-2 gnd Enzymkatalyse (DE-588)4152480-9 gnd Galactosidase beta- (DE-588)4336804-9 gnd Molke (DE-588)4140861-5 gnd Ballaststoff (DE-588)4004346-0 gnd Hydrolyse (DE-588)4160918-9 gnd |
| subject_GND | (DE-588)4155521-1 (DE-588)4235506-0 (DE-588)4132717-2 (DE-588)4152480-9 (DE-588)4336804-9 (DE-588)4140861-5 (DE-588)4004346-0 (DE-588)4160918-9 (DE-588)4113937-9 |
| title | The enzymatic synthesis of prebiotic lactulose in dairy matrices set-up, analysis, process implementation and health effects |
| title_auth | The enzymatic synthesis of prebiotic lactulose in dairy matrices set-up, analysis, process implementation and health effects |
| title_exact_search | The enzymatic synthesis of prebiotic lactulose in dairy matrices set-up, analysis, process implementation and health effects |
| title_full | The enzymatic synthesis of prebiotic lactulose in dairy matrices set-up, analysis, process implementation and health effects Regina Lucie Schuster-Wolff-Bühring |
| title_fullStr | The enzymatic synthesis of prebiotic lactulose in dairy matrices set-up, analysis, process implementation and health effects Regina Lucie Schuster-Wolff-Bühring |
| title_full_unstemmed | The enzymatic synthesis of prebiotic lactulose in dairy matrices set-up, analysis, process implementation and health effects Regina Lucie Schuster-Wolff-Bühring |
| title_short | The enzymatic synthesis of prebiotic lactulose in dairy matrices |
| title_sort | the enzymatic synthesis of prebiotic lactulose in dairy matrices set up analysis process implementation and health effects |
| title_sub | set-up, analysis, process implementation and health effects |
| topic | Fructose (DE-588)4155521-1 gnd Milchprodukt (DE-588)4235506-0 gnd Zusatz (DE-588)4132717-2 gnd Enzymkatalyse (DE-588)4152480-9 gnd Galactosidase beta- (DE-588)4336804-9 gnd Molke (DE-588)4140861-5 gnd Ballaststoff (DE-588)4004346-0 gnd Hydrolyse (DE-588)4160918-9 gnd |
| topic_facet | Fructose Milchprodukt Zusatz Enzymkatalyse Galactosidase beta- Molke Ballaststoff Hydrolyse Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024740279&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV000021889 |
| work_keys_str_mv | AT schusterwolffbuhringregina theenzymaticsynthesisofprebioticlactuloseindairymatricessetupanalysisprocessimplementationandhealtheffects |