Single-Molecule Microscopy Study of Nano-Systems: From synthetic photo-switchable nano-devices to the dynamics of naturally occurring transcription factors:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
München
Dr. Hut
2012
|
| Ausgabe: | 1. Aufl. |
| Schriftenreihe: | Physikalische Chemie
|
| Schlagworte: | |
| Online-Zugang: | Inhaltstext https://nbn-resolving.org/urn:nbn:de:bvb:19-138640 Volltext Inhaltsverzeichnis |
| Beschreibung: | IV, 174 S. Ill., graph. Darst. |
| ISBN: | 9783843902809 3843902801 |
Internformat
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Datensatz im Suchindex
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IMAGE 1
CONTENTS
1. FLUORESCENCE 1
1.1. FUNDAMENTALS 1
1.2. FLUORESCENCE ANISOTROPY 5
1.3. FOERSTER RESONANCE ENERGY TRANSFER 6
2. TOTAL INTERNAL REFLECTION MICROSCOPY 11
2.1. FUNDAMENTALS 11
2.2. TOTAL INTERNAL REFLECTION 11
2.3. EXPERIMENTAL SETUP 15
2.3.1. TIRF PRISM 18
2.3.2. EMCCD 20
3. DATA ANALYSIS 27
3.1. MAPPING 27
3.2. EXTRACTION OF SINGLE-MOLECULE INTENSITIES 33
3.3. SIGNAL CORRECTION 35
3.4. THE HIDDEN MARKOV MODEL 41
4. SINGLE-MOLECULE PHOTOSWITCHABLE NANODEVICE 55 4.1. INTRODUCTION 55
4.2. FLUORESCENT PROTEIN DRONPA 56
4.2.1. STRUCTURE 58
4.3. SAMPLE PREPARATION 58
4.4. DRONPA AS A FRET SWITCH 61
4.4.1. SINGLE-MOLECULE EXPERIMENTS 61
4.4.2. LIVE-CELL IMAGING MEASUREMENTS 67
4.4.3. DRONPA AS PH SENSOR 74
5. MODULATION OF TBP BINDING TO THE TATA-BOX BY MOTI 85 5.1.
INTRODUCTION 85
5.1.1. PROTEIN BIOSYNTHESIS 86
BIBLIOGRAFISCHE INFORMATIONEN HTTP://D-NB.INFO/1019448210
DIGITALISIERT DURCH
IMAGE 2
IV CONTENTS
5.1.2. THETATA-BOX 87
5.1.3. THE TATA-BOX BINDING PROTEIN 87
5.1.4. THE MOTI PROTEIN 89
5.2. INTERACTION OF MOTI WITH THE TBP/DNA BINARY COMPLEX 91 5.2.1.
CONFORMATIONAL CHANGES OF THE TBP/DNA COMPLEX . . 91 5.2.2. MODULATION
OF THE TATA-BOX CONFORMATION 99
5.2.3. KINETIC OF TATA-BOX BENDING 117
5.2.4. DISSOCIATION OFTBP FROM THE TATA-BOX 123
5.3. DISCUSSION 129
6. SUMMARY AND OUTLOOK 135
A. APPENDICES 137
A.L. PROTEIN LABELING 137
A.2. BENDING ANGLE CALCULATION 137
A.3. DNA SEQUENCES 138
A.4. INFLUENCE OF FRET ON PHOTO-BLEACHING 138
A.5. TRACE INTENSITY ANALYSIS 140
A.6. TDP KINETIC RATES FITS 143 |
| any_adam_object | 1 |
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| dewey-ones | 572 - Biochemistry |
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| dewey-search | 572.88450282 |
| dewey-sort | 3572.88450282 |
| dewey-tens | 570 - Biology |
| discipline | Chemie / Pharmazie Biologie |
| edition | 1. Aufl. |
| format | Thesis Book |
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| isbn | 9783843902809 3843902801 |
| language | English |
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| physical | IV, 174 S. Ill., graph. Darst. |
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| spelling | Heiss, Gregor Verfasser aut Single-Molecule Microscopy Study of Nano-Systems: From synthetic photo-switchable nano-devices to the dynamics of naturally occurring transcription factors Gregor Heiss 1. Aufl. München Dr. Hut 2012 IV, 174 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Physikalische Chemie Zugl.: München, Ludwig-Maximilians-Univ., Diss., 2011 Einzelmolekülmikroskopie (DE-588)7669710-1 gnd rswk-swf Startreaktion (DE-588)4210216-9 gnd rswk-swf Transkription Genetik (DE-588)4185906-6 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Transkription Genetik (DE-588)4185906-6 s Startreaktion (DE-588)4210216-9 s Einzelmolekülmikroskopie (DE-588)7669710-1 s DE-604 Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:19-138640 X:MVB text/html http://deposit.dnb.de/cgi-bin/dokserv?id=3972939&prov=M&dok_var=1&dok_ext=htm Verlag kostenfrei Inhaltstext https://nbn-resolving.org/urn:nbn:de:bvb:19-138640 Resolving-System http://edoc.ub.uni-muenchen.de/13864/ Verlag kostenfrei Volltext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024733812&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Heiss, Gregor Single-Molecule Microscopy Study of Nano-Systems: From synthetic photo-switchable nano-devices to the dynamics of naturally occurring transcription factors Einzelmolekülmikroskopie (DE-588)7669710-1 gnd Startreaktion (DE-588)4210216-9 gnd Transkription Genetik (DE-588)4185906-6 gnd |
| subject_GND | (DE-588)7669710-1 (DE-588)4210216-9 (DE-588)4185906-6 (DE-588)4113937-9 |
| title | Single-Molecule Microscopy Study of Nano-Systems: From synthetic photo-switchable nano-devices to the dynamics of naturally occurring transcription factors |
| title_auth | Single-Molecule Microscopy Study of Nano-Systems: From synthetic photo-switchable nano-devices to the dynamics of naturally occurring transcription factors |
| title_exact_search | Single-Molecule Microscopy Study of Nano-Systems: From synthetic photo-switchable nano-devices to the dynamics of naturally occurring transcription factors |
| title_full | Single-Molecule Microscopy Study of Nano-Systems: From synthetic photo-switchable nano-devices to the dynamics of naturally occurring transcription factors Gregor Heiss |
| title_fullStr | Single-Molecule Microscopy Study of Nano-Systems: From synthetic photo-switchable nano-devices to the dynamics of naturally occurring transcription factors Gregor Heiss |
| title_full_unstemmed | Single-Molecule Microscopy Study of Nano-Systems: From synthetic photo-switchable nano-devices to the dynamics of naturally occurring transcription factors Gregor Heiss |
| title_short | Single-Molecule Microscopy Study of Nano-Systems: From synthetic photo-switchable nano-devices to the dynamics of naturally occurring transcription factors |
| title_sort | single molecule microscopy study of nano systems from synthetic photo switchable nano devices to the dynamics of naturally occurring transcription factors |
| topic | Einzelmolekülmikroskopie (DE-588)7669710-1 gnd Startreaktion (DE-588)4210216-9 gnd Transkription Genetik (DE-588)4185906-6 gnd |
| topic_facet | Einzelmolekülmikroskopie Startreaktion Transkription Genetik Hochschulschrift |
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