Rho GTPases: methods and protocols
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York [u.a.]
Humana Press
2012
|
| Schriftenreihe: | Methods in molecular biology
827 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XIV, 407 S. Ill., graph. Darst. |
| ISBN: | 9781617794414 1617794414 |
Internformat
MARC
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| 300 | |a XIV, 407 S. |b Ill., graph. Darst. | ||
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Datensatz im Suchindex
| _version_ | 1804148792963891200 |
|---|---|
| adam_text | Titel: Rho GTPases
Autor: Rivero, Francisco
Jahr: 2012
Contents
Preface.......................................................... v
Contributors...................................................... xi
Part I History and Phylogenesis
1 Historical Overview of Rho GTPases................................ 3
Anne J. Ridley
2 Rho GTPases: Deciphering the Evolutionary History
of a Complex Protein Family...................................... 13
Marek Elias and Vladimir Klimes
Part II Biochemistry of Rho GTPases
3 Biochemical Assays to Characterize Rho GTPases...................... 37
Mamta Jaiswal, Badri N. Dubey, Katja T. Koessmeier,
Lothar Gremer, and Mohammad R. Ahmadian
4 How to Analyze Bacterial Toxins Targeting Rho GTPases................ 59
Heike Bielek and Gudula Schmidt
5 Assessing Ubiquitylation of Rho GTPases in Mammalian Cells............ 77
Anne Doye, Amel Mettouchi, and Emmanuel Lemichez
6 Posttranslational Lipid Modification of Rho Family Small GTPases......... 87
Natalia Mitin, Patrick J. Roberts, Emily J. Chenette,
and ChanningJ. Der
7 Analysis of the Role of RhoGDIl and Isoprenylation
in the Degradation of RhoGTPases................................. 97
Etienne Boulter and Rafael Garcia-Mata
8 A Quantitative Fluorometric Approach for Measuring the Interaction
of RhoGDI with Membranes and Rho GTPases....................... 107
Jared Johnson, Richard A. Cerione, and Jon W. Erickson
Part III Functional Assays
9 Rho GTPases and Cancer Cell Transendothelial Migration............... 123
Nicolas Reymond, Philippe Riou, and Anne J. Ridley
10 Quantitative and Robust Assay to Measure Cell-Cell Contact
Assembly and Maintenance....................................... 143
Sebastien Nola, Jennifer C. Erasmus, and Vania M.M. Braga
11 Rho GTPase Knockout Induction in Primary Keratinocytes
from Adult Mice............................................... 157
Esben Pedersen, Astrid Basse, Tine Lefever, Karine Peyrollier,
and Cord Brakebusch
vii
viii Contents
12 Rho GTPase Techniques in Osteoclastogenesis........................ 167
Roland Leung and Michael Glogauer
13 Assessment of Rho GTPase Signaling During Neurite Outgrowth.......... 181
Daniel Feltrin and Olivier Pertz
14 Assessment of the Role for Rho Family GTPases in NADPH
Oxidase Activation............................................. 195
Kei Miyano and Hideki Sumimoto
Part TV Imaging and High Throughput Methods
15 Multiplex Imaging of Rho Family GTPase Activities in Living Cells......... 215
Desiree Spiering and Louis Hodgson
16 FRET-Based Imaging of Rac and Cdc42 Activation During
Fc-Receptor-Mediated Phagocytosis in Macrophages.................... 235
Adam D. Hoppe
17 High-Throughput Flow Cytometry Bead-Based Multiplex Assay
for Identification of Rho GTPase Inhibitors.......................... 253
Zurab Surviladze, Susan M. Young, and Larry A. Sklar
18 Functional Analysis of Rho GTPase Activation and Inhibition
in a Bead-Based Miniaturized Format............................... 271
Michael Schmohl, Stefanie Rimmele, Peter Gierschik,
Thomas O. Joos, and Nicole Schneiderhan-Marra
19 Use of Phage Display for the Identification of Molecular Sensors
Specific for Activated Rho........................................ 283
Patrick Chinestra, Isabelle Lajoie-Mazenc,
Jean-Charles Faye, and Gilles Favre
20 Identification of New Interacting Partners for Atypical
Rho GTPases: A SILAC-Based Approach............................ 305
Laura Montani, Damaris Bausch-Fluck, Ana Filipa Domingues,
Bernd Wollscheid, and Joäo Bettencourt Relvas
Part V Rho GTPases in Non-Mammalian Model Organisms
21 Using Zebrafish for Studying Rho GTPases Signaling In Vivo............. 321
Shizhen Zhu and Boon Chuan Low
22 Analysis of Rho GTPase Function in Axon Pathfinding
Using Caenorhabditis elegans...................................... 339
Jamie K. Alan and Erik A. Lundquist
23 Protocol for Ex Vivo Incubation of Drosophila Primary
Post-embryonic Haemocytes for Real-Time Analyses.................... 359
Christopher J. Sampson and Michael J. Williams
24: Analysis of Rho GTPase Activation in Saccharomyces cerevisiae............. 369
Gary Eitzen and Michael R. Logan
Contents ix
25 Assaying Rho GTPase-Dependent Processes
in Dictyostelium discoideum....................................... 381
Huajiang Xiong and Francisco Rivero
26 FRAP-Based Analysis of Rho GTPase-Dependent Polar
Exocytosis in Pollen Tubes....................................... 393
An Tan and Zhenbiao Tang
Index........................................................... 403
|
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| id | DE-604.BV039856255 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T00:12:46Z |
| institution | BVB |
| isbn | 9781617794414 1617794414 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-024715905 |
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| physical | XIV, 407 S. Ill., graph. Darst. |
| publishDate | 2012 |
| publishDateSearch | 2012 |
| publishDateSort | 2012 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | Rho GTPases methods and protocols ed. by Francisco Rivero New York [u.a.] Humana Press 2012 XIV, 407 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 827 Springer protocols Rho-Proteine (DE-588)4681926-5 gnd rswk-swf Rho-Proteine (DE-588)4681926-5 s DE-604 Rivero, Francisco Sonstige oth Methods in molecular biology 827 (DE-604)BV035362695 827 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024715905&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Rho GTPases methods and protocols Methods in molecular biology Rho-Proteine (DE-588)4681926-5 gnd |
| subject_GND | (DE-588)4681926-5 |
| title | Rho GTPases methods and protocols |
| title_auth | Rho GTPases methods and protocols |
| title_exact_search | Rho GTPases methods and protocols |
| title_full | Rho GTPases methods and protocols ed. by Francisco Rivero |
| title_fullStr | Rho GTPases methods and protocols ed. by Francisco Rivero |
| title_full_unstemmed | Rho GTPases methods and protocols ed. by Francisco Rivero |
| title_short | Rho GTPases |
| title_sort | rho gtpases methods and protocols |
| title_sub | methods and protocols |
| topic | Rho-Proteine (DE-588)4681926-5 gnd |
| topic_facet | Rho-Proteine |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024715905&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035362695 |
| work_keys_str_mv | AT riverofrancisco rhogtpasesmethodsandprotocols |