The role of LRP2 in embryonic development of zebrafish and mouse:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2012
|
| Schlagworte: | |
| Online-Zugang: | kostenfrei Inhaltsverzeichnis |
| Beschreibung: | Nebentitel: Die Rolle von LRP2 bei der Embryonalentwicklung von Zebrafisch und Maus |
| Beschreibung: | XIV, 107 S. Ill., graph. Darst. |
Internformat
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV039852094 | ||
| 003 | DE-604 | ||
| 005 | 00000000000000.0 | ||
| 007 | t | ||
| 008 | 120130s2012 gw ad|| m||| 00||| eng d | ||
| 035 | |a (OCoLC)849798690 | ||
| 035 | |a (DE-599)BVBBV039852094 | ||
| 040 | |a DE-604 |b ger |e rakwb | ||
| 041 | 0 | |a eng | |
| 044 | |a gw |c DE | ||
| 049 | |a DE-188 | ||
| 082 | 0 | |a 570 | |
| 100 | 1 | |a Kur, Esther |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a The role of LRP2 in embryonic development of zebrafish and mouse |c vorgelegt von Esther Kur |
| 246 | 1 | 3 | |a Die Rolle von LRP2 bei der Embryonalentwicklung von Zebrafisch und Maus |
| 264 | 1 | |c 2012 | |
| 300 | |a XIV, 107 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Nebentitel: Die Rolle von LRP2 bei der Embryonalentwicklung von Zebrafisch und Maus | ||
| 502 | |a Berlin, Freie Univ., Diss., 2011 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 776 | 0 | 8 | |i Erscheint auch als |n Online-Ausgabe |o urn:nbn:de:kobv:188-fudissthesis000000035490-4 |
| 856 | 4 | 1 | |u http://www.diss.fu-berlin.de/diss/receive/FUDISS_thesis_000000035490 |z kostenfrei |3 Volltext |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024711809&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 912 | |a ebook | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-024711809 | ||
Datensatz im Suchindex
| _version_ | 1804148787436847104 |
|---|---|
| adam_text | IMAGE 1
CONTENTS
VI LIST OF ABBREVIATIONS
VIII LIST OF FIGURES
X LIST OF TABLES
XI SUMMARY
XIII ZUSAMMENFASSUNG
1 1. INTRODUCTION
1 1.1 THE LDLR FAMILY
1 1.1.1 STRUCTURAL H A L L M A R K S O F LDLR FAMILY M E M B E R S
2 1.1.2 EVOLUTION O F T H E LDLR FAMILY
2 1.1.3 FUNCTIONS O F LDLR FAMILY M E M B E R S
2 FUNCTIONS IN T H E A D U L T O R G A N I S M
5 FUNCTIONS IN T H E D E V E L O P I N G O R G A N I S M
6 1.2 LRP2
6 1.2.1 I N V E R T E B R A T E A N I M A L M O D E L S F O R LRP2
DEFICIENCY
7 1.2.2 LRP2 DEFICIENT ZEBRAFISH
7 1.2.3 LRP2 DEFICIENT M I C E
7 I M P A I R E D R E N A L P R O T E I N R E A B S O R P T I O N IN
LRP2 / M I C E
8 I M P A I R E D SEX H O R M O N E SIGNALING IN LRP2~ / ~ M I C E
8 DEFECTIVE F O R E B R A I N D E V E L O P M E N T IN LRP2~ / ~ M I C E
10 1.2.4 LRP2 M U T A T I O N S IN H U M A N S
10 1.3 FOREBRAIN DEVELOPMENT AND MIDLINE SPECIFICATION
11 1.3.1 F O R E B R A I N I N D U C T I O N
12 1.3.2 N E U R U L A T I O N
13 1.3.3 MIDLINE SPECIFICATION A N D D O R S O V E N T R A L P A T T E R
N I N G
13 SHH SIGNALS F R O M T H E V E N T R A L M I D L I N E
1 4 FGF SIGNALS F R O M T H E A N T E R I O R N E U R A L R I D G E
1 4 BMP A N D WNT SIGNALS F R O M T H E D O R S A L M I D L I N E
15 DEREGULATION O F F O R E B R A I N P A T T E R N I N G C A N R E S U
L T IN HPE
1 6 2. AIM
17 3. RESULTS
17 3.1 ZEBRAFISH LRP2 IS CRUCIAL FOR KIDNEY FUNCTION, BUT NOT FOR
FOREBRAIN DEVELOPMENT
17 3.1.1 DETAILED E X P R E S S I O N ANALYSIS O F LRP2 IN T H E
ZEBRAFISH
HTTP://D-NB.INFO/102568009X
IMAGE 2
CONTENTS
17 ZEBRAFISH LRP2 R E C A P I T U L A T E S SPATIAL A S P E C T S O F M
A M M A L I A N LRP2
E X P R E S S I O N
19 ZEBRAFISH LRP2 D O E S N O T R E C A P I T U L A T E T E M P O R A L
A S P E C T S OF
M A M M A L I A N LRP2 E X P R E S S I O N
20 3.1.2 ANALYSIS O F LRP2 DEFICIENT ZEBRAFISH
20 BUGEYE A N D 5CBEN ZEBRAFISH LINES A R E DEFICIENT FOR LRP2
21 LOSS O F LRP2 D I S R U P T S CLEARANCE P A T H W A Y S IN T H E
ZEBRAFISH
E M B R Y O N I C K I D N E Y
25 LOSS O F LRP2 D O E S N O T I M P A I R F O R E B R A I N P A T T E R
N I N G IN ZEBRAFISH
30 3.1.3 ZEBRAFISH LRP2B D O E S N O T C O M P E N S A T E FOR LOSS O F
LRP2
30 T H E G E N E LRP2B E N C O D E S A R E C E P T O R H O M O L O G U E
U N I Q U E T O FISH
32 LRP2B IS D I S P E N S A B L E FOR C L E A R A N C E P A T H W A Y S
IN T H E ZEBRAFISH K I D N E Y
3 4 SIMULTANEOUS LOSS O F LRP2 A N D L R P 2 B D O E S N O T R E S U L T
IN A B R A I N
P H E N O T Y P E
35 3.2 LRP2 IS REQUIRED FOR THE SPECIFICATION OF MIDLINE CELLS DURING
MOUSE FOREBRAIN DEVELOPMENT
35 3.2.1 A N E W M O U S E M O D E L T O S T U D Y T H E FUNCTION O F
LRP2: L R P2 TM ( ECFPCRE )
35 GENERATION O F T H E I R P2 TM ( EGFPCRE ) LINE
3 8 CRE ACTIVITY INDICATES E A R L Y O N S E T O F LPR2 E X P R E S S I
O N
3 9 EGFP E X P R E S S I O N IS A R E A D - O U T FOR LRP2 P R O M O T E
R ACTIVITY
4 1 LRP2 P R O M O T E R ACTIVITY IS H I G H E R IN T H E V E N T R A L
T H A N IN T H E D O R S A L
MIDLINE
4 2 LRP2 P R O M O T E R ACTIVITY IS D E C R E A S E D IN LRP2 DEFICIENT
M I C E
4 3 VENTRAL MIDLINE CELLS A R E PARTICULARLY AFFECTED B Y LRP2
DEFICIENCY
4 5 LOW LRP2 P R O M O T E R ACTIVITY P R E V E N T S S O R T I N G O F
EGFP POSITIVE CELLS
4 6 3.2.2 EXPRESSION PROFILING O F WILD T Y P E A N D LRP2 DEFICIENT
MIDLINE CELLS
4 7 DISSECTION O F DEFINED LRP2 E X P R E S S I N G CELL P O P U L A T I
O N S FOR
E X P R E S S I O N PROFILING
4 8 EXPRESSION PROFILING REVEALS C H A N G E S IN G E N E E X P R E S S
I O N IN LRP2
DEFICIENT E M B R Y O S C O M P A R E D T O C O N T R O L S
52 ALL M A J O R SIGNALING C E N T E R S IN T H E DEVELOPING F O R E B R
A I N A R E
D I S T U R B E D IN LRP2 DEFICIENT E M B R Y O S
5 4 T H E T E L E N C E P H A L O N IS N O T CORRECTLY SPECIFIED IN LRP2
DEFICIENT
E M B R Y O S
56 4. DISCUSSION
5 6 4.1 LRP2 IN ZEBRAFISH DEVELOPMENT
5 6 4.1.1 LRP2 IS T H E M A I N ENDOCYTOSIS R E C E P T O R IN T H E
ZEBRAFISH P R O N E P H R O S
57 4.1.2 LRP2 IS N O T CRUCIAL FOR F O R E B R A I N D E V E L O P M E N
T O F T H E ZEBRAFISH
57 IS LOSS O F LRP2 FUNCTION C O M P E N S A T E D B Y O T H E R LDLR
FAMILY M E M B E R S ?
II
IMAGE 3
61
62
6 5
66
66
6 7
7 0
7 0
7 0
7 0
7 0
7 0
7 1
7 1
7 1
7 1
7 2
7 2
7 2
7 3
7 4
7 4
7 4
7 4
7 5
7 5
7 6
7 6
CONTENTS
DIFFERENT K E Y A S P E C T S IN V E N T R A L MIDLINE I N D U C T I O N
IN ZEBRAFISH A N D
M A M M A L S
4.2 LRP2 IN MOUSE DEVELOPMENT
4.2.1 N E W INSIGHTS INTO LRP2 E X P R E S S I O N A N D ITS R E G U L A
T I O N
EARLY O N S E T O F LRP2 E X P R E S S I O N IN M I C E
T H E V E N T R A L M I D L I N E IS T H E M A J O R E X P R E S S I O N
D O M A I N O F LRP2 IN T H E
F O R E B R A I N
REGULATION O F LRP2 E X P R E S S I O N
4.2.2 A ROLE FOR LRP2 IN CONTROLLING T H E M O R P H O L O G Y O F V E N
T R A L M I D L I N E CELLS
4.2.3 A DETAILED A C C O U N T O F T H E G E N E E X P R E S S I O N
DEFECTS IN T H E LRP2 DEFICIENT F O R E B R A I N
EXPRESSION O F ALL F O U R K E Y M O R P H O G E N S IS A L T E R E D IN
LRP2 DEFICIENT
E M B R Y O S A T E9.5
DORSALIZATION A N D E X P A N S I O N O F DIENCEPHALIC I D E N T I T Y L
E A D T O
I M P A I R E D I N D U C T I O N O F T H E T E L E N C E P H A L O N IN
LRP2 DEFICIENT E M B R Y O S
3. MATERIAL & METHODS
3.1 MATERIAL
3.1.1 OLIGONUCLEOTIDES
MOUSE SPECIFIC P R I M E R S E Q U E N C E S
ZEBRAFISH SPECIFIC P R I M E R S E Q U E N C E S
MORPHOLINO S E Q U E N C E S
QPCR P R O B E S E Q U E N C E S
3.1.2 ISH P R O B E S
MOUSE SPECIFIC ISH P R O B E S
ZEBRAFISH SPECIFIC ISH P R O B E S
3.1.3 ANTIBODIES
3.1.4 MEDIA
3.1.5 BUFFERS A N D S O L U T I O N S
3.1.6 KITS
3.1.7 TECHNICAL E Q U I P M E N T
3.1.8 CHEMICALS
3.2 ANIMAL EXPERIMENTS
3.2.1 MOUSE H U S B A N D R Y
3.2.2 ZEBRAFISH H U S B A N D R Y
3.2.3 MORPHOLINO INJECTIONS
3.2.4 DYE FILTRATION A N D U P T A K E E X P E R I M E N T S
3.3 MOLECULAR BIOLOGY METHODS
IMAGE 4
CONTENTS
7 6 3.3.1 CLONING
76 ENZYMATIC D I G E S T O F DNA
76 AMPLIFICATION O F DNA F R A G M E N T S B Y PCR
76 AGAROSE GEL E L E C T R O P H O R E S I S O F DNA A N D RNA
77 D E T E R M I N A T I O N DNA A N D RNA C O N C E N T R A T I O N
77 LIGATION O F DNA F R A G M E N T S
77 T R A N S F O R M A T I O N O F B A C T E R I A W I T H DNA
77 CRYOPRESERVATION O F B A C T E R I A
78 ISOLATION O F P L A S M I D DNA F R O M B A C T E R I A
78 SEQUENCING O F DNA
78 3.3.2 GENOTYPING
78 ISOLATION O F G E N O M I C DNA FOR S O U T H E R N BLOT
79 ISOLATION O F G E N O M I C DNA FOR PCR (HOT SHOT)
79 GENOTYPING B Y PCR
79 GENOTYPING B Y S O U T H E R N BLOT
80 ISOLATION O F TOTAL RNA FROM T I S S U E S A M P L E S
80 FIRST S T R A N D CDNA S Y N T H E S I S
80 QUANTITATIVE RT PCR (QPCR)
8 0 3.3.6 FLOW C Y T O M E T R Y
81 3.3.7 LASER C A P T U R E MICRODISSECTION
81 3.3.8 GENE E X P R E S S I O N PROFILING
81 P R E P A R A T I O N O F LABELED CDNA/CRNA
81 MICROARRAY
81 STATISTICS
8 2 3.4 HISTOLOGY AND STAININGS
82 3.4.1 TISSUE SECTIONS
82 PARAFFIN SECTIONS
82 PLASTIC SECTIONS
82 CRYOSECTIONS
82 VIBRATOME SECTIONS
83 3.4.2 HISTOLOGICAL STAININGS
83 ORANGE G S T A I N I N G
83 H&E STAINING
83 3.4.3 I M M U N O H I S T O C H E M I S T R Y
83 FLUORESCENT I M M U N O H I S T O C H E M I S T R Y O N CRYOSECTIONS
83 DAB I M M U N O H I S T O C H E M I S T R Y O N PARAFFIN SECTIONS
8 4 I M M U N O H I S T O C H E M I S T R Y O N W H O L E - M O U N T
ZEBRAFISH E M B R Y O S
8 4 3.4.4 IN SITU HYBRIDIZATION
8 4 GENERATION O F DIGOXIGENIN-LABELED RNA P R O B E S
8 4 W H O L E - M O U N T ISH O N M O U S E E M B R Y O S
8 5 W H O L E - M O U N T ISH O N ZEBRAFISH E M B R Y O S
IV
IMAGE 5
8 5
8 5
8 7
8 7
8 7
8 7
8 7
88
88
88 88
8 9
8 9
8 9
9 0
9 7
CONTENTS
3.5 MICROSCOPY
3.5.1 CONFOCAL M I C R O S C O P Y
3.5.2 ELECTRON M I C R O S C O P Y
3.6 GENERATION OF KNOCK-IN MOUSE MUTANTS
3.6.1 KNOCK-IN V E C T O R C O N S T R U C T I O N
3.6.2 ES CELL C U L T U R E
CULTIVATION O F ES CELLS
ELECTROPORATION O F ES CELLS
ISOLATION O F ES CELL CLONES
FREEZING O F ES CELL CLONES
3.6.3 INJECTION O F E M B R Y O N I C S T E M CELL CLONES INTO
BLASTOCYSTS
3.7 IN SILICO ANALYSES
3.7.1 PHYLOGENETIC T R E E
3.7.2 IDENTIFICATION O F T R A N S C R I P T I O N FACTOR B I N D I N G
S I T E S
6. APPENDIX
7. BIBLIOGRAPHY
V
|
| any_adam_object | 1 |
| author | Kur, Esther |
| author_facet | Kur, Esther |
| author_role | aut |
| author_sort | Kur, Esther |
| author_variant | e k ek |
| building | Verbundindex |
| bvnumber | BV039852094 |
| collection | ebook |
| ctrlnum | (OCoLC)849798690 (DE-599)BVBBV039852094 |
| dewey-full | 570 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 570 - Biology |
| dewey-raw | 570 |
| dewey-search | 570 |
| dewey-sort | 3570 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01506nam a2200361 c 4500</leader><controlfield tag="001">BV039852094</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">00000000000000.0</controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">120130s2012 gw ad|| m||| 00||| eng d</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)849798690</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV039852094</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="044" ind1=" " ind2=" "><subfield code="a">gw</subfield><subfield code="c">DE</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-188</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">570</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Kur, Esther</subfield><subfield code="e">Verfasser</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">The role of LRP2 in embryonic development of zebrafish and mouse</subfield><subfield code="c">vorgelegt von Esther Kur</subfield></datafield><datafield tag="246" ind1="1" ind2="3"><subfield code="a">Die Rolle von LRP2 bei der Embryonalentwicklung von Zebrafisch und Maus</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2012</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">XIV, 107 S.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">Nebentitel: Die Rolle von LRP2 bei der Embryonalentwicklung von Zebrafisch und Maus</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="a">Berlin, Freie Univ., Diss., 2011</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Online-Ausgabe</subfield><subfield code="o">urn:nbn:de:kobv:188-fudissthesis000000035490-4</subfield></datafield><datafield tag="856" ind1="4" ind2="1"><subfield code="u">http://www.diss.fu-berlin.de/diss/receive/FUDISS_thesis_000000035490</subfield><subfield code="z">kostenfrei</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024711809&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ebook</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-024711809</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV039852094 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T00:12:41Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-024711809 |
| oclc_num | 849798690 |
| open_access_boolean | 1 |
| owner | DE-188 |
| owner_facet | DE-188 |
| physical | XIV, 107 S. Ill., graph. Darst. |
| psigel | ebook |
| publishDate | 2012 |
| publishDateSearch | 2012 |
| publishDateSort | 2012 |
| record_format | marc |
| spelling | Kur, Esther Verfasser aut The role of LRP2 in embryonic development of zebrafish and mouse vorgelegt von Esther Kur Die Rolle von LRP2 bei der Embryonalentwicklung von Zebrafisch und Maus 2012 XIV, 107 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Nebentitel: Die Rolle von LRP2 bei der Embryonalentwicklung von Zebrafisch und Maus Berlin, Freie Univ., Diss., 2011 (DE-588)4113937-9 Hochschulschrift gnd-content Erscheint auch als Online-Ausgabe urn:nbn:de:kobv:188-fudissthesis000000035490-4 http://www.diss.fu-berlin.de/diss/receive/FUDISS_thesis_000000035490 kostenfrei Volltext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024711809&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Kur, Esther The role of LRP2 in embryonic development of zebrafish and mouse |
| subject_GND | (DE-588)4113937-9 |
| title | The role of LRP2 in embryonic development of zebrafish and mouse |
| title_alt | Die Rolle von LRP2 bei der Embryonalentwicklung von Zebrafisch und Maus |
| title_auth | The role of LRP2 in embryonic development of zebrafish and mouse |
| title_exact_search | The role of LRP2 in embryonic development of zebrafish and mouse |
| title_full | The role of LRP2 in embryonic development of zebrafish and mouse vorgelegt von Esther Kur |
| title_fullStr | The role of LRP2 in embryonic development of zebrafish and mouse vorgelegt von Esther Kur |
| title_full_unstemmed | The role of LRP2 in embryonic development of zebrafish and mouse vorgelegt von Esther Kur |
| title_short | The role of LRP2 in embryonic development of zebrafish and mouse |
| title_sort | the role of lrp2 in embryonic development of zebrafish and mouse |
| topic_facet | Hochschulschrift |
| url | http://www.diss.fu-berlin.de/diss/receive/FUDISS_thesis_000000035490 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024711809&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT kuresther theroleoflrp2inembryonicdevelopmentofzebrafishandmouse AT kuresther dierollevonlrp2beiderembryonalentwicklungvonzebrafischundmaus |