The role of the matrix regulator Fra-1 in arthritis and liver fibrosis: = Die Rolle des Matrixregulators Fra-1 in Arthritis und Leberfibrose
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| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
2011
|
| Subjects: | |
| Online Access: | Volltext https://nbn-resolving.org/urn:nbn:de:bvb:29-opus-28487 Inhaltsverzeichnis |
| Physical Description: | II, 92 S. Ill., graph. Darst. 21 cm |
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| 245 | 1 | 0 | |a The role of the matrix regulator Fra-1 in arthritis and liver fibrosis |b = Die Rolle des Matrixregulators Fra-1 in Arthritis und Leberfibrose |c vorgelegt von Trayana Kireva |
| 246 | 1 | 1 | |a Die Rolle des Matrixregulators Fra-1 in Arthritis und Leberfibrose |
| 264 | 1 | |c 2011 | |
| 300 | |a II, 92 S. |b Ill., graph. Darst. |c 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Erlangen-Nürnberg, Univ., Diss., 2011 | ||
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| 912 | |a ebook | ||
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Record in the Search Index
| _version_ | 1804148732741025792 |
|---|---|
| adam_text | TABLE
OF
CONTENTS
ZUSAMMENFASSUNG
.................................................................................................
I
SUMMARY
......................................................................................................................
3
1
INTRODUCTION
.....................................................................................................
5
1.1
THE
AP-1
COMPLEX................
-
----
.
-----------------------
.....
-
...
------
-
...
---
...
-
......
-
.......
5
1.2
BONE
DEVELOPMENT
......................
-
..........
___
...
-
--------------
-
-
...
-------------
...
-------------
---------
7
1.3
BONE
DESTRUCTION
DURING
ARTHRITIS
-----------------
-----------------------------
-
----------
-------------------
10
1.4
LIVER
DEVELOPMENT
...
___
....
-----
-----
.
-----
---------------------------
---------------------------------------------
12
1.5
LIVER
INJURY
13
1.6
LIVER
FIBROSIS
-----------------------
....
---------------------
-------..-----
--------------------
...
--------------
--------
14
1.7
CHOLESTATIC
LIVER
DISEASES
--------
-----------------------------
...........
--------------------------------------------
....
16
2
AIM
OF
THE
STUDY
.............................................................................................
18
3
MATERIAL
............................................................................................................
20
3.1
BIOLOGICAL
MATERIAL:.....
-------------------
-
-
------------------------------------------------------------------
20
3.1.1
ANIMALS
........................................................................................................................................................
20
3.1.2
PRIMARY
CELLS
................................................................................................................................................
20
3.1.3
HUMAN
TISSUE
..............................................................................................................................................20
3.2
NON-BIOLOGICAL
MATERIAL:......
--------------
......
---------------------------------------------
-
-
-----
............
21
3.2.1
CHEMICALS,
MEDIA
AND
BUFFER
.......................................................................................................................
21
3.2.2
CYTOKINES
AND
ENZYMES
...............................................................................................................................
22
3.2.3
PRIMERS
.........................................................................................................................................................
22
3.2.4
ANTIBODIES
....................................................................................................................................................22
3.2.5
KITS
...............................................................................................................................................................
22
3.2.6
SOFTWARE
.......................................................................................................................................................
22
3.2.7
INSTRUMENTS
...................................................................................................................................................23
4
METHODS
............................................................................................................
24
4.1
METHODS
PERFORMED
TO
STUDY
THE
BONE
PHENOTYPE
OF
FRA-LTG
X
HTNFTG
MICE
---------------------------
24
4.1.1
CLINICAL
ASSESSMENT
....................................................................................................................................
24
4.1.2
BONE
HISTOMORPHOMETRY
.............................................................................................................................24
4.1.3
BONE
HISTOLOGY
............................................................................................................................................24
4.1.4
IN
SITU
HYBRIDISATION
...................................................................................................................................25
4.1.5
ELISA
.............................................................................................................................................................25
4.1.6
ISOLATION
AND
CULTURE
OF
OSTEOCLAST
PRECURSORS
...........................................................................................
26
4.1.7
ISOLATION
AND
CULTURE
OF
CHONDROCYTES
........................................................................................................
26
4.2
METHODS
PERFORMED
TO
STUDY
LIVER
PATHOLOGY
OF
FRA-LTG
MICE
----------------
....
26
4.2.1
LIVER
HISTOLOGY
.............................................................................................................................................
26
4.2.2
SIRIUS
RED
STAINING
.........................................................................................................................................27
4.2.3
ALKALINE
PHOSPHATASE
ACTIVITY
....................................................................................................................27
4.2.4
HYDROXYPROLINE
ASSAY
.................................................................................................................................27
4.2.5
CHROMATINE
IMMUNOPRECIPITATION
(CHIP)
.................................................................................................27
4.2.6
RT
2
PROFILER
PCR
ARRAY
...............................................................................................................................28
4.2.7
ISOLATION
OF
NON-PARENCHYMAL
LIVER
CELLS
AND
BLOOD
CELLS
.........................................................................
28
4.2.8
FACS
ANALYSIS
.............................................................................................................................................29
4.3
GENERAL
METHODS
-------------------------------------------------------------------------------------------
----
......
___29
4.3.1
GENOTYPING
OF
ANIMALS
...............................................................................................................................
29
4.3.2
RT-PCR
.......................................................................................................................................................
29
4.3.3
IMMUNOHISTOCHEMISTRY
................................................................................................................................30
4.4
STATISTICAL
ANALYSIS.............
30
5
RESULTS
..............................................................................................................
31
5.1
THE
ROLE
OF
FRA-1
IN
BONE
AND
CARTILAGE
HOMEOSTASIS
DURING
CHRONIC
ARTHRITIS
________________
31
5.1.1
CLINICAL
FEATURES
OF
FRA-LTG
X
HTNFTG
MICE
.............................................................................................
31
5.1.2
SYNOVIAL
INFLAMMATION
IN
FRA-LTG
X
HTNFTG
MICE
..................................................................................
33
5.1.3
FRA-1
PREVENTS
LOCAL
BONE
DAMAGE
IN
HTNFTG
MICE
.................................................................................
35
5.1.4
FRA-LTG
X
HTNFTG
MICE
DEVELOP
SYSTEMIC
OSTEOSCLEROSIS
.......................................................................
37
5.1.5
FRA-1
PROTECTS
FROM
TNF-INDUCED
CARTILAGE
DEGRADATION
..........................................................................
40
5.1.6
INCREASED
PROLIFERATION
OF
PRIMARY
CHONDROCYTES
OVEREXPRESSING
FRA-1
.................................................
43
5.2
FRA-LTG
MICE
DEVELOP
LIVER
FIBROSIS
....................................
45
5.2.1
FRA-LTG
MICE
DEVELOP
PROGRESSIVE
HEPATIC
PORTAL
INFLAMMATION
AND
DUCTULAR
REACTION
........................45
5.2.2
HEPATIC
UP-REGULATION
OF
CHEMOKINES
IN
FRA-LTG
MICE
...........................................................................48
5.2.3
HEPATIC
INFILTRATION
OF
ACTIVATED
T
CELLS
AND
NEUTROPHILS
IN
FRA-LTG
MICE
.............................................
50
5.2.4
FRA-LTG
MICE
DEVELOP
PROGRESSIVE
BILIARY
CIRRHOSIS
..................................................................................
54
5.2.5
UPREGULATION
OF
PRO-FIBROTIC
GENES
IN
LIVERS
OF
FRA-LTG
MICE
..................................................................
56
5.2.6
CO-LOCALIZATION
OF
FRA-1
AND
PRO-FIBROTIC
PROTEINS
IS
CONFINED
TO
CHOLANGIOCYTES
IN
FRA-LTG
MICE....
58
5.2.7
FRA-1
EXPRESSION
IN
HUMAN
SAMPLES
OF
LIVER
FIBROSIS
................................................................................60
5.2.8
INVOLVEMENT
OF
T
CELLS
IN
THE
PROGRESSION
OF
LIVER
FIBROSIS
IN
FRA-LTG
MICE
........................................
62
6
DISCUSSION
........................................................................................................
64
REFERENCES
.............................................................................................................
73
APPENDIX
...................................................................................................................
83
ABBREVATIONS
.........................................................................................................
88
ACKNOWLEDGEMENT
...............................................................................................
90
CURRICULUM
VITAE
..................................................................................................
91
|
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| author_GND | (DE-588)1018317457 |
| author_facet | Kireva, Trayana 1980- |
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| author_sort | Kireva, Trayana 1980- |
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| institution | BVB |
| language | English |
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| physical | II, 92 S. Ill., graph. Darst. 21 cm |
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| spelling | Kireva, Trayana 1980- Verfasser (DE-588)1018317457 aut The role of the matrix regulator Fra-1 in arthritis and liver fibrosis = Die Rolle des Matrixregulators Fra-1 in Arthritis und Leberfibrose vorgelegt von Trayana Kireva Die Rolle des Matrixregulators Fra-1 in Arthritis und Leberfibrose 2011 II, 92 S. Ill., graph. Darst. 21 cm txt rdacontent n rdamedia nc rdacarrier Erlangen-Nürnberg, Univ., Diss., 2011 (DE-588)4113937-9 Hochschulschrift gnd-content Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:29-opus-28487 https://open.fau.de/handle/openfau/1978 Verlag kostenfrei Volltext https://nbn-resolving.org/urn:nbn:de:bvb:29-opus-28487 Resolvingsystem DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024664076&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Kireva, Trayana 1980- The role of the matrix regulator Fra-1 in arthritis and liver fibrosis = Die Rolle des Matrixregulators Fra-1 in Arthritis und Leberfibrose |
| subject_GND | (DE-588)4113937-9 |
| title | The role of the matrix regulator Fra-1 in arthritis and liver fibrosis = Die Rolle des Matrixregulators Fra-1 in Arthritis und Leberfibrose |
| title_alt | Die Rolle des Matrixregulators Fra-1 in Arthritis und Leberfibrose |
| title_auth | The role of the matrix regulator Fra-1 in arthritis and liver fibrosis = Die Rolle des Matrixregulators Fra-1 in Arthritis und Leberfibrose |
| title_exact_search | The role of the matrix regulator Fra-1 in arthritis and liver fibrosis = Die Rolle des Matrixregulators Fra-1 in Arthritis und Leberfibrose |
| title_full | The role of the matrix regulator Fra-1 in arthritis and liver fibrosis = Die Rolle des Matrixregulators Fra-1 in Arthritis und Leberfibrose vorgelegt von Trayana Kireva |
| title_fullStr | The role of the matrix regulator Fra-1 in arthritis and liver fibrosis = Die Rolle des Matrixregulators Fra-1 in Arthritis und Leberfibrose vorgelegt von Trayana Kireva |
| title_full_unstemmed | The role of the matrix regulator Fra-1 in arthritis and liver fibrosis = Die Rolle des Matrixregulators Fra-1 in Arthritis und Leberfibrose vorgelegt von Trayana Kireva |
| title_short | The role of the matrix regulator Fra-1 in arthritis and liver fibrosis |
| title_sort | the role of the matrix regulator fra 1 in arthritis and liver fibrosis die rolle des matrixregulators fra 1 in arthritis und leberfibrose |
| title_sub | = Die Rolle des Matrixregulators Fra-1 in Arthritis und Leberfibrose |
| topic_facet | Hochschulschrift |
| url | https://open.fau.de/handle/openfau/1978 https://nbn-resolving.org/urn:nbn:de:bvb:29-opus-28487 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024664076&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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