Hepatitis E virus in German domestic pigs: occurrence and prevalence:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Gießen
DVG-Service
2011
|
| Ausgabe: | 1. Aufl. |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | VIII, 94 S. Ill., graph. Darst. 22 cm |
| ISBN: | 9783863450564 |
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Datensatz im Suchindex
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| adam_text | IMAGE 1
CONTENTS
CONTENTS
1 . INTRODUCTION 1
2. LITERATURE REVIEW 4
2.1. HEPATITIS E VIRUS 4
2.1.1. TAXONOMY AND GENOTYPES 4
2.1.2. MORPHOLOGY 5
2.1.3. GENETIC STRUCTURE AND VIRAL PROTEINS 5
2.2. HEPATITIS E VIRUS INFECTION OF HUMANS 7
2.2.1. TRANSMISSION AND EPIDEMIOLOGY 7
2.2.2. CLINICAL SYMPTOMS, COURSE OF INFECTION AND DIAGNOSIS 8
2.2.3. PATHOGENESIS 10
2.2.4. THERAPY 11
2.2.5. PREVENTION AND CONTROL 12
2.3. HEPATITIS E VIRUS INFECTION OF DOMESTIC PIGS 13
2.3.1. TRANSMISSION AND EPIDEMIOLOGY 13
2.3.2. CLINICAL SIGNS, DIAGNOSIS AND COURSE OF INFECTION 14
2.3.3. EXPERIMENTAL HEPATITIS E VIRUS INFECTION OF PIGS 17
2.4. HEPATITIS E VIRUS INFECTION OF FURTHER ANIMAL SPECIES 19
2.4.1. HEPATITIS E VIRUS INFECTION OF WILD BOARS AND DEER 19
2.4.2. HEPATITIS E VIRUS INFECTION OF RODENTS AND OTHER ANIMAL SPECIES
20 2.4.3. HEPATITIS E VIRUS INFECTION OF CHICKEN 21
2.5. HEPATITIS E - A ZOONOSIS 21
3. PREVALENCE OF HEPATITIS E VIRUS-SPECIFIC ANTIBODIES IN SERA OF GERMAN
DOMESTIC PIGS ESTIMATED BY DIFFERENT ASSAYS 24
3.1. ABSTRACT 25
4. MOLECULAR CHARACTERISATION OF HEPATITIS E VIRUS INFECTION IN GERMAN
DOMESTIC PIGS 26
4.1. ABSTRACT 27
4.2. INTRODUCTION 28
4.3. MATERIALS AND METHODS 30
4.3.1. LIVER SAMPLES 30
4.3.2. ISOLATION OF RNA 30
4.3.3. REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION (RT-PCR) 30
4.3.4. CONSTRUCTION OF CRNA STANDARDS 31
4.3.5. REVERSE TRANSCRIPTASE-QUANTITATIVE PCR (RT-QPCR) 32
4.3.6. HISTOPATHOLOGICAL STUDIES 33
4.3.7. GENETIC TYPING AND PHYLOGENETIC ANALYSIS 33
4.3.8. STATISTICAL ANALYSES 33
4.4. RESULTS 34
4.4.1. RT-PCR 34
4.4.2. SEQUENCES AND PHYLOGENETIC ANALYSES 35
4.4.3. QUANTIFICATION OF VIRAL RNA LEVELS USING REAL TIME PCR 37
4.4.4. HISTOLOGY 38
BIBLIOGRAFISCHE INFORMATIONEN HTTP://D-NB.INFO/1018150374
DIGITALISIERT DURCH
IMAGE 2
CONTENTS
4.5. DISCUSSION 38
4.6. TABLE AND FIGURES 43
5. INFECTION OF DOMESTIC PIGS WITH A HUMAN HEPATITIS E VIRUS STRAIN -
PATHOHISTOLOGY AND DETECTION OF VIRAL RNA IN ORGANS AND TISSUES 49 5.1.
ABSTRACT 50
5.2. INTRODUCTION 51
5.3. MATERIALS AND METHODS 52
5.3.1. ANIMALS 52
5.3.2. HEPATITIS E VIRUS INOCULUM 53
5.3.3. STUDY DESIGN 53
5.3.4. RNA ISOLATION 53
5.3.5. RT-PCR AND SEQUENCE ANALYSIS 54
5.3.6. HISTOLOPATHOLOGY 54
5.4. RESULTS 55
5.4.1. CLINICAL EXAMINATION 55
5.4.2. RT-PCR 55
5.4.3. SEQUENCE ANALYSIS 56
5.4.4. MACROSCOPIC LESIONS 56
5.4.5. HISTOPATHOLOGY 56
5.5. DISCUSSION 57
5.6. TABLE AND FIGURES 61
6. OVERALL DISCUSSION 65
6.1 . SEROLOGICAL AND BIOMOLECULAR INVESTIGATIONS ON HEPATITIS E VIRUS
PREVALENCE IN GERMAN DOMESTIC PIGS 65
6.2. CHARACTERISTICS OF HEV INFECTION OF DOMESTIC PIGS 68
6.3. DOMESTIC PIGS AS HEV RESERVOIR - RELEVANCE FOR ZOONOTIC
INFECTIONS?... 71
7. SUMMARY 75
8. ZUSAMMENFASSUNG 77
9. BIBLIOGRAPHY 79
|
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| dewey-full | 636.40896362301 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 636 - Animal husbandry |
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| dewey-tens | 630 - Agriculture and related technologies |
| discipline | Agrar-/Forst-/Ernährungs-/Haushaltswissenschaft / Gartenbau Medizin |
| edition | 1. Aufl. |
| format | Thesis Book |
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| institution | BVB |
| isbn | 9783863450564 |
| language | English |
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| physical | VIII, 94 S. Ill., graph. Darst. 22 cm |
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| spelling | Bächlein, Christine Verfasser (DE-588)1018202560 aut Hepatitis E virus in German domestic pigs: occurrence and prevalence by Christine Bächlein 1. Aufl. Gießen DVG-Service 2011 VIII, 94 S. Ill., graph. Darst. 22 cm txt rdacontent n rdamedia nc rdacarrier Zugl.: Hannover, Tierärztl. Hochsch., Diss., 2011 hepatitis E virus cabt Germany cabt pigs cabt occurrence cabt prevalence cabt PCR cabt ELISA cabt liver diseases cabt Deutschland (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024660488&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Bächlein, Christine Hepatitis E virus in German domestic pigs: occurrence and prevalence hepatitis E virus cabt Germany cabt pigs cabt occurrence cabt prevalence cabt PCR cabt ELISA cabt liver diseases cabt |
| subject_GND | (DE-588)4113937-9 |
| title | Hepatitis E virus in German domestic pigs: occurrence and prevalence |
| title_auth | Hepatitis E virus in German domestic pigs: occurrence and prevalence |
| title_exact_search | Hepatitis E virus in German domestic pigs: occurrence and prevalence |
| title_full | Hepatitis E virus in German domestic pigs: occurrence and prevalence by Christine Bächlein |
| title_fullStr | Hepatitis E virus in German domestic pigs: occurrence and prevalence by Christine Bächlein |
| title_full_unstemmed | Hepatitis E virus in German domestic pigs: occurrence and prevalence by Christine Bächlein |
| title_short | Hepatitis E virus in German domestic pigs: occurrence and prevalence |
| title_sort | hepatitis e virus in german domestic pigs occurrence and prevalence |
| topic | hepatitis E virus cabt Germany cabt pigs cabt occurrence cabt prevalence cabt PCR cabt ELISA cabt liver diseases cabt |
| topic_facet | hepatitis E virus Germany pigs occurrence prevalence PCR ELISA liver diseases Deutschland Hochschulschrift |
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