Verfügbarkeit: Handbook of ELISPOT

Handbook of ELISPOT: methods and protocols

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Bibliographische Detailangaben
Format:	Buch
Sprache:	English
Veröffentlicht:	New York, NY [u.a.] Humana Press, Springer 2012
Ausgabe:	2. ed.
Schriftenreihe:	Methods in molecular biology 792
Schlagworte:	Enzyme-linked immunosorbent assay Aufsatzsammlung
Online-Zugang:	Klappentext Inhaltsverzeichnis
Beschreibung:	Includes bibliographical references and index
Beschreibung:	X, 261 S. Ill., graph. Darst.
ISBN:	9781617793240

Internformat

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Datensatz im Suchindex

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adam_text	Contents Preface .............................................................. γ Contributors. ......................................................... ix Part I Setting and Performing ELISPOT Assay 1 Unique Strengths of ELISPOT for Τ Cell Diagnostics .................,___ 3 Paul V. Lehmann and Wenfi Zhang 2 The Impact of Harmonization on ELISPOT Assay Performance ............... 25 Sylvia Janetzki and Cedrik M. Britten Part II ELISPOT for Veterinary Research 3 Equine ELISPOT Assay to Study Secretion of IFNy and IL-4 from Peripheral Blood Mononuclear Cells .................................___....... 39 Jodi Hagen, Chris Hartnett, Jeffrey P. Houchins, Steeve Giguire, and Alexander E, Kalyttzfmy 4 Utilization of Peline ELISPOT for Mapping Vaccine Epitopes ................ 47 Jeffrey R, Abbotts Ruiyn Pu, James К. Coleman, and Janet К, Tataamoto В Analyzing Cellular Immunity to AAV in a Canine Model Using ELISPOT Assay ___ .......................................... 65 Zeßng Wang, Rainer Starb, Stefibe» J. Taprntt, and Stanley Riddell Part III Advanced ELISPOT Techniques 6 Dual- and Triple-Color Fhtorospot ..................................... 77 Niklas Ablborg aud Bernt Axelsson 7 ELISPOT Assay as a Tool to Study Oxidative Stress in Lymphocytes. ........... 87 Jodi Hagen, Jeffrey P. Homhins, and Alexander E. Kalyusdiny 8 ELISPOT Assay for Neurosciencc Research: Studying TNFa Secretion from Microglial Cells ............................................... 97 Jedi Hagen, Jeffrey P. Hombins, and Alexander E. Kaly uzliny 9 ELI SPOT Assay as a Tool to Stady the Effects of Stem Celts on Cytokine Secretion .............................................. 105 Jun-SeopShm and Chung-Gym Park Ш Combining ELISPOT and ELISA to Measure Amounts of Cytokines Secreted by a Single Celi ....................... —.................... 1 IB Jedi Hägern^ Jeffrey R Hembins, mid Alexander Ш. vu viii Contents Part IV Image and Data Analysis 11 How ELISPOT Morphology Reflects on the Productivity and Kinetics of Cells Secretory Activity ........................................... 125 Alexey Ύ. Karulin and Paul V. Lehmann 12 Mathematical Algorithms for Automatic Search, Recognition, and Detection of Spots in ELISPOT Assay ............................... 145 Sergey S. Zadorozhny and Nikolai N. Martynov 13 Objective, User-Independent ELISPOT Data Analysis Based on Scientifically Validated Principles .................................... 155 Wenji Zhang and Paul V. Lehmann 14 Statistical Analysis of ELISPOT Assays .................................. 173 Marcus Oittrich and Paul V. Lehmann 15 Response Determination Criteria for ELISPOT: Toward a Standard that Can Be Applied Across Laboratories ................................ 185 Zoe Moodie, Leah Price, Sylvia Janetzki, and Cedrik M. Britten Part V ELISPOT Assay for Vaccine Development and Diagnostics 16 Detection of Vaccinia Virus-Specific IFNy and IL-10 Secretion from Human PBMCs and CD8+ Τ Cells by ELISPOT ...................... 199 Benjamin J . Umlauf, Norman A. Pinsky, Inna G. Ovsy annikova, and Gregory A. Poland 17 ELISPOT Assays to Enumerate Bovine IHSJ-y-Secreting Cells for the Development of Novel Vaccines Against Bovine Tuberculosis ........... 219 Martin Vordermeier and Adam O. Whelan 18 IL-7 Addition Increases Spot Size and Number as Measured byT-SPOT.ÏB® ................................................... 229 Marsha L. Feske, Miguel Medina, Edward A. Graviss, and Dorothy Ћ. Lewis 19 Overview of Membranes and Membrane Plates Used in Research and Diagnostic ELISPOT Assays ...................................... 243 AlanJ. Weiss Index ............................................................... 257 Methods in Molecular Biology 792 John M. Walker, Series Editor Alexander E. Kalyuzhny Editor Handbook of ELISPOT Methods and Protocols Second Edition Enzyme-linked immunospot assay { ELISPOT) has been known for some time as a unique state- of-the-art technique for studying the cytokine-secreting activity of immune system cells, and it appears to be one of the fast growing applications in biomedical research, becoming an indis¬ pensable tool in vaccine development, HIV research, transplantation studies, and cancer and allergy research. The second edition oí Handbook of HIJSPOT: Methods and Protocols, only the second book in the field which is entirely dedicated to ELISPOT assay, shares the detailed techniques that have been developed since the release of the popular first edition. Straight from the labs of seasoned experts, this book covers setting and performing ELISPOT assays, ELIS¬ POT for veterinary research, advanced ELISPOT techniques, image and data analysis, as well as vaccine development and diagnostics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Handbook of ELISPOT: Methods and Protocols, Second Edition serves as a compilation of a technical reference and a troubleshooting guide for researchers, both experienced and novice, worldwide in order to advance the usage of this key tool.
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discipline	Medizin
edition	2. ed.
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spelling	Handbook of ELISPOT methods and protocols ed. by Alexander E. Kalyuzhny 2. ed. New York, NY [u.a.] Humana Press, Springer 2012 X, 261 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 792 Springer protocols Includes bibliographical references and index Enzyme-linked immunosorbent assay Enzyme-linked immunosorbent assay (DE-588)4152476-7 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Enzyme-linked immunosorbent assay (DE-588)4152476-7 s DE-604 Kalyuzhny, Alexander E. Sonstige oth Erscheint auch als Online-Ausgabe 978-1-61779-325-7 Methods in molecular biology 792 (DE-604)BV035362695 792 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024624465&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Klappentext Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024624465&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
spellingShingle	Handbook of ELISPOT methods and protocols Methods in molecular biology Enzyme-linked immunosorbent assay Enzyme-linked immunosorbent assay (DE-588)4152476-7 gnd
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title	Handbook of ELISPOT methods and protocols
title_auth	Handbook of ELISPOT methods and protocols
title_exact_search	Handbook of ELISPOT methods and protocols
title_full	Handbook of ELISPOT methods and protocols ed. by Alexander E. Kalyuzhny
title_fullStr	Handbook of ELISPOT methods and protocols ed. by Alexander E. Kalyuzhny
title_full_unstemmed	Handbook of ELISPOT methods and protocols ed. by Alexander E. Kalyuzhny
title_short	Handbook of ELISPOT
title_sort	handbook of elispot methods and protocols
title_sub	methods and protocols
topic	Enzyme-linked immunosorbent assay Enzyme-linked immunosorbent assay (DE-588)4152476-7 gnd
topic_facet	Enzyme-linked immunosorbent assay Aufsatzsammlung
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