Ribosome display and related technologies: Methods and protocols
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York [u.a.]
Humana Press
2012
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| Schriftenreihe: | Methods in molecular biology
805 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XVI, 424 S. graph. Darst. |
| ISBN: | 9781617793783 |
Internformat
MARC
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| 245 | 1 | 0 | |a Ribosome display and related technologies |b Methods and protocols |c ed. by Julie A. Douthwaite ... |
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Datensatz im Suchindex
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| adam_text | Titel: Ribosome display and related technologies
Autor: Douthwaite, Julie A.
Jahr: 2012
Contents
Preface.............................................................. v
Contributors.......................................................... x¿
Part I Review
1 Ribosome Display: A Perspective...................................... 3
Andreas Pliicktbun
Part II Translation Extract Preparation
2 Preparation and Testing of E. coli S30 In Vitro Transcription
Translation Extracts................................................ 31
James F. Zawada
Part III Basic Ribosome Display and Related Selection Methods
3 Eukaryotic Ribosome Display Selection Using Rabbit
Reticulocyte Lysate................................................. 45
Julie A. Douthwaite
4 Stabilized Ribosome Display for In Vitro Selection......................... 59
Shuta Ham, Mingzhe Liu, Wei Wang, Muye Xu, Zha Li,
and Toshihiro Ito
5 Eukaryotic Ribosome Display with In Situ DNA Recovery................... 75
Mingyue He, Bryan M. Edwards, Damjana Kastelic,
and Michael J. Taussig
6 mRNA Display Using Covalent Coupling of mRNA
to Translated Proteins............................................... 87
Rong Wang, Steve W. Gotten, and Rihe Liu
7 SNAP Display: In Vitro Protein Evolution in Microdroplets.................. 101
Miriam Kaltenbach and Florian Hollfelder
8 cDNA Display: Rapid Stabilization of mRNA Display....................... 113
Shingo Ueno and Naoto Nemoto
Part IV Applications of Ribosome Display Methods
Using Natural Amino Acids
9 Optimisation of Antibody Affinity by Ribosome Display
Using Error-Prone or Site-Directed Mutagenesis.......................... 139
Leeanne Lewis and Chris Lloyd
10 Affinity Maturation of Phage Display Antibody Populations
Using Ribosome Display............................................. 163
Maria A. Groves and Adrian A. Nickson
¡X
x Contents
11 Evolution of Protein Stability Using Ribosome Display...................... 191
Andrew Buchanan
12 Selection of Lead Antibodies from Naive Ribosome
Display Antibody Libraries........................................... 213
Peter Ravn
13 Evolution of Disulfide-Rich Peptide Aptamers Using cDNA Display............ 237
Tuki Mochizuki and Naoto Nemoto
14 Peptide Screening Using PURE Ribosome Display......................... 251
Hiroyuki Ohashi, Takashi Kanamori, Eriko Osada,
Bintang K. Akbar, and Takuya Ueda
15 Rapid Selection of High-Affinity Binders Using Ribosome Display............. 261
Birgit Dreier and Andreas Plilckthun
16 mRNA Display-Based Selections Using Synthetic Peptide
and Natural Protein Libraries......................................... 287
Steve W. Gotten, Jianwei Zou, Rang Wang, Bao-cheng Huang,
and Rihe Liu
17 Identification of Candidate Vaccine Genes Using Ribosome Display............ 299
Liancheng Lei
18 Ribosome Display for the Selection of Sac7d Scaffolds...................... 315
Barbara Mouratou, Ghislaine Béhar, Lauranne Paillard-Laurance,
Stéphane Colinet, and Frederic Pecorari
Part V Incorporation of Non-Natural Amino Acids for Selection
by Ribosome Display and Related Methods
19 Charging of tRNAs Using Ribozymes and Selection
of Cyclic Peptides Containing Thioethers................................ 335
Patrick C. Reid, Yuki Goto, Takayuki Katoh, and Hiroaki Suga
20 Update on Pure Translation Display with Unnatural Amino
Acid Incorporation................................................. 349
R. Edward Watts and Anthony C. Forster
21 In Vitro Selection of Unnatural Cyclic Peptide Libraries
via mRNA Display................................................. 367
Zhong Ma and Matthew CT. Hartman
Part VI Case Studies
22 Optimization of CAT-354, a Therapeutic Antibody Directed
Against Interleukin-13, Using Ribosome Display.......................... 393
George Thorn and Ralph Minter
23 Affinity Maturation and Functional Dissection of a Humanised
Anti-RAGE Monoclonal Antibody by Ribosome Display..................... 403
Simon E. Hufton
Jndex............................................................... 423
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| publishDate | 2012 |
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| series | Methods in molecular biology |
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| spelling | Ribosome display and related technologies Methods and protocols ed. by Julie A. Douthwaite ... New York [u.a.] Humana Press 2012 XVI, 424 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 805 Ribosom (DE-588)4127731-4 gnd rswk-swf Molekularbiologische Methode (DE-588)7650063-9 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Ribosom (DE-588)4127731-4 s Molekularbiologische Methode (DE-588)7650063-9 s b DE-604 Douthwaite, Julie A. edt Methods in molecular biology 805 (DE-604)BV035362695 805 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024537320&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
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| title | Ribosome display and related technologies Methods and protocols |
| title_auth | Ribosome display and related technologies Methods and protocols |
| title_exact_search | Ribosome display and related technologies Methods and protocols |
| title_full | Ribosome display and related technologies Methods and protocols ed. by Julie A. Douthwaite ... |
| title_fullStr | Ribosome display and related technologies Methods and protocols ed. by Julie A. Douthwaite ... |
| title_full_unstemmed | Ribosome display and related technologies Methods and protocols ed. by Julie A. Douthwaite ... |
| title_short | Ribosome display and related technologies |
| title_sort | ribosome display and related technologies methods and protocols |
| title_sub | Methods and protocols |
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