Secretome analysis in higher basidiomycetes: freely secreted and cell wall proteins from Coprinopsis cinerea
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Clausthal-Zellerfeld
Papierflieger-Verl.
2011
|
| Ausgabe: | 1. Aufl. |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Zsfassung in dt. Sprache. - Enth. Zeitschriftenaufsätze |
| Beschreibung: | XIII, 244 S. Ill., graph. Darst. |
| ISBN: | 9783869481746 |
Internformat
MARC
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| 003 | DE-604 | ||
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| 100 | 1 | |a Güttel, Dorothea |d 1982- |e Verfasser |0 (DE-588)1015789110 |4 aut | |
| 245 | 1 | 0 | |a Secretome analysis in higher basidiomycetes |b freely secreted and cell wall proteins from Coprinopsis cinerea |c submitted by Dorothea Güttel |
| 250 | |a 1. Aufl. | ||
| 264 | 1 | |a Clausthal-Zellerfeld |b Papierflieger-Verl. |c 2011 | |
| 300 | |a XIII, 244 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Zsfassung in dt. Sprache. - Enth. Zeitschriftenaufsätze | ||
| 502 | |a Zugl.: Göttingen, Univ., Diss., 2010 | ||
| 650 | 0 | 7 | |a Extrazellulärraum |0 (DE-588)4205506-4 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Proteomanalyse |0 (DE-588)4596545-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Sekretorische Proteine |0 (DE-588)4728729-9 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Coprinus cinereus |0 (DE-588)4391978-9 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Coprinus cinereus |0 (DE-588)4391978-9 |D s |
| 689 | 0 | 1 | |a Sekretorische Proteine |0 (DE-588)4728729-9 |D s |
| 689 | 0 | 2 | |a Extrazellulärraum |0 (DE-588)4205506-4 |D s |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-024499004 | ||
Datensatz im Suchindex
| _version_ | 1804148507831959552 |
|---|---|
| adam_text | IMAGE 1
CONTENTS
ZUSAMMENFASSUNG I
SUMMARY IV
ACKNOWLEDGEMENT VII
ACKNOWLEDGEMENT TO CONTRIBUTIONS OF OTHERS TO THIS WORK IX
1 GENERAL INTRODUCTION 1
1.1 THE FUNGAL CELL WALL ARCHITECTURE 1
1.1.1 THE FUNGAL CELL WALL STRUCTURE - STATE OF THE ART 2
1.1.2 FUNGAL CELL WALL PROTEINS 6
1.1.2.1 NON-COVALENTLY BOUND CELL WALL PROTEINS 7
1.1.2.2 COVALENTLY BOUND CELL WALL PROTEINS 10
1.2 SECRETED PROTEINS FROM HIGHER BASIDIOMYCETES 14
1.3 FUNGAL PROTEOMICS - STATE OF THE ART 16
1.4 GENOMES OF HIGHER BASIDIOMYCETES 18
1.4.1 TECHNIQUES FOR GENOME SEQUENCING 19
1.4.2 ALREADY SEQUENCED GENOMES OF AGARICOMYCOTINA 22
1.4.3 GENOMES IN SEQUENCING AND ANNOTATION PROGRESS 24
1.4.4 APPLICATION OF WHOLE GENOME SEQUENCES 27
1.5 OBJECTIVE OF THE THESIS 29
2 OPTIMIZED 2-DE PROTOCOL 53
3 THE SECRETOME OF COPRINOPSIS CINEREA 65
3.1 ABSTRACT 65
3.2 INTRODUCTION 66
BIBLIOGRAFISCHE INFORMATIONEN HTTP://D-NB.INFO/1015396186
DIGITALISIERT DURCH
IMAGE 2
CONTENTS
3.3 MATERIAL AND METHODS 68
3.3.1 CHEMICALS 68
3.3.2 FUNGAL CULTURES 69
3.3.3 PROTEIN FRACTIONATION 69
3.3.4 PROTEIN PRECIPITATION 70
3.3.5 PROTEIN QUANTIFICATION 71
3.3.6 PROTEIN SEPARATION BY 2-DE 71
3.3.7 MASS SPECTROMETRY AND IDENTIFICATION OF 2-DE SPOTS 72
3.3.8 PROTEIN SEPARATION AND LC-MS 2 FOR THE SHOTGUN ANALYSIS 74
3.3.9 SHOTGUN DATA ANALYSIS AND PROTEIN IDENTIFICATION 74
3.4 RESULTS 76
3.4.1 2-DE ANALYSIS OF THE SECRETED PROTEIN FRACTIONS 77
3.4.2 1-DE SHOTGUN PROTEOMICS OF THE SECRETOME 83
3.4.2.1 PROTEIN IDENTIFICATION 95
3.4.3 SEARCH FOR POTENTIAL PIR AND GPI PROTEINS OF C. CINEREA 96
3.4.3.1 PIR PROTEINS 96
3.4.3.2 GPI-ANCHORED PROTEINS 97
3.5 DISCUSSION 98
3.5.1 2-DE ANALYSIS OF THE FRACTIONATED SECRETOME FROM COPRINOPSIS
CINEREA 99
3.5.2 1-DE SHOTGUN APPROACH 101
3.5.2.1 EXTRACELLULAR OXIDOREDUCTASES 101
3.5.2.2 GLYCOSIDE HYDROLASES 103
3.5.2.3 PEPTIDASES 104
3.5.2.4 OTHER HYDROLASES 105
3.5.2.5 POSSIBLE INTRACELLULAR CONTAMINATIONS AND MOONLIGHTING
PROTEINS 106
3.5.2.6 UNKNOWN PROTEINS 107
3.5.3 IN SILICO PREDICTION OF PUTATIVE PIR AND GPI PROTEINS 108
3.5.3.1 PIR-PROTEINS 108
3.5.3.2 GPI-ANCHORED PROTEINS 109
3.5.4 CONCLUSIONS AND FUTURE OUTLOOK 110
4 POSTTRANSLATIONAL MODIFICATIONS OF THE SECRETOME 126
IMAGE 3
CONTENTS
4.1 ABSTRACT 126
4.2 INTRODUCTION 127
4.3 MATERIAL AND METHODS 129
4.3.1 CHEMICALS 129
4.3.2 FUNGAL CULTURES 129
4.3.3 PROTEIN EXTRACTION AND IDENTIFICATION 129
4.4 RESULTS 130
4.4.1 PHOSPHORYLATED PROTEINS OCCUR IN THE SECRETOME OF C. CINEREA . .
130
4.4.2 THE NATURE OF PHOSPHORYLATION OF SECRETED PROTEINS 139
4.5 DISCUSSION 139
4.5.1 DEGLYCOSYLATION OF THE SECRETED PROTEINS 141
4.5.2 PHOSPHORYLATED PROTEINS OCCUR IN THE SECRETOME OF C. CINEREA . .
141
5 THE SECRETOME OF C. CINEREA DURING GROWTH 149
5.1 ABSTRACT 149
5.2 INTRODUCTION 149
5.3 MATERIAL AND METHODS 151
5.3.1 CHEMICALS 151
5.3.2 CULTURE CONDITIONS 151
5.3.3 DETERMINATION OF THE FUNGAL BIOMASS 152
5.3.4 DETERMINATION OF THE PROTEIN AND GLUCOSE CONCENTRATION 152
5.3.5 PROTEIN EXTRACTION AND IDENTIFICATION 153
5.4 RESULTS 153
5.4.1 GROWTH OF C. CINEREA IN LIQUID CULTURE OVER THE TIME 153
5.4.2 COMPARISON OF THE FRACTIONATED SECRETOME OF THE DIFFERENT GROWTH
PHASES 157
5.4.3 PROTEIN IDENTIFICATION 164
5.5 DISCUSSION 169
5.5.1 THE FRACTIONATED SECRETOME OF C. CINEREA IS REDUCED IN COM-
PLEXITY DURING GROWTH 171
5.5.2 IDENTIFICATION OF PROTEIN SPOTS 172
5.5.3 C. CINEREA POSSESSES A DYNAMIC SECRETOME 177
6 GENERAL DISCUSSION 182
IMAGE 4
CONTENTS
6.1 STRATEGIES FOR PROTEIN IDENTIFICATION 183
6.1.1 PUTATIVE INTRACELLULAR ENZYMES WERE DETECTED IN THE CELL WALL. .
184 6.2 THE SECRETOME OF COPRINOPSIS CINEREA SHOWS A DISTINCT
COMPARTMENTATIONL85 6.3 ENZYMES INVOLVED IN NUTRIENT SUPPLY 188
6.4 PUTATIVE PROTEINS INVOLVED IN THE CONSTRUCTION OF THE CELL WALL AND
THE HYPHAL SHEATH 192
6.5 ANALYSIS OF THE SECRETOME FROM PLEUROTUS OSTREATUS ON STRAW AS
NATURAL SUBSTRATE 194
6.6 FUTURE PERSPECTIVE 200
A APPENDIX 210
ABBREVIATIONS 242
|
| any_adam_object | 1 |
| author | Güttel, Dorothea 1982- |
| author_GND | (DE-588)1015789110 |
| author_facet | Güttel, Dorothea 1982- |
| author_role | aut |
| author_sort | Güttel, Dorothea 1982- |
| author_variant | d g dg |
| building | Verbundindex |
| bvnumber | BV039649292 |
| classification_tum | BIO 272d BIO 327d |
| ctrlnum | (OCoLC)760139312 (DE-599)BVBBV039649292 |
| discipline | Biologie |
| edition | 1. Aufl. |
| format | Thesis Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV039649292 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T00:08:14Z |
| institution | BVB |
| isbn | 9783869481746 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-024499004 |
| oclc_num | 760139312 |
| open_access_boolean | |
| owner | DE-M49 DE-BY-TUM DE-Eb1 |
| owner_facet | DE-M49 DE-BY-TUM DE-Eb1 |
| physical | XIII, 244 S. Ill., graph. Darst. |
| publishDate | 2011 |
| publishDateSearch | 2011 |
| publishDateSort | 2011 |
| publisher | Papierflieger-Verl. |
| record_format | marc |
| spelling | Güttel, Dorothea 1982- Verfasser (DE-588)1015789110 aut Secretome analysis in higher basidiomycetes freely secreted and cell wall proteins from Coprinopsis cinerea submitted by Dorothea Güttel 1. Aufl. Clausthal-Zellerfeld Papierflieger-Verl. 2011 XIII, 244 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zsfassung in dt. Sprache. - Enth. Zeitschriftenaufsätze Zugl.: Göttingen, Univ., Diss., 2010 Extrazellulärraum (DE-588)4205506-4 gnd rswk-swf Proteomanalyse (DE-588)4596545-6 gnd rswk-swf Sekretorische Proteine (DE-588)4728729-9 gnd rswk-swf Coprinus cinereus (DE-588)4391978-9 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Coprinus cinereus (DE-588)4391978-9 s Sekretorische Proteine (DE-588)4728729-9 s Extrazellulärraum (DE-588)4205506-4 s Proteomanalyse (DE-588)4596545-6 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024499004&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Güttel, Dorothea 1982- Secretome analysis in higher basidiomycetes freely secreted and cell wall proteins from Coprinopsis cinerea Extrazellulärraum (DE-588)4205506-4 gnd Proteomanalyse (DE-588)4596545-6 gnd Sekretorische Proteine (DE-588)4728729-9 gnd Coprinus cinereus (DE-588)4391978-9 gnd |
| subject_GND | (DE-588)4205506-4 (DE-588)4596545-6 (DE-588)4728729-9 (DE-588)4391978-9 (DE-588)4113937-9 |
| title | Secretome analysis in higher basidiomycetes freely secreted and cell wall proteins from Coprinopsis cinerea |
| title_auth | Secretome analysis in higher basidiomycetes freely secreted and cell wall proteins from Coprinopsis cinerea |
| title_exact_search | Secretome analysis in higher basidiomycetes freely secreted and cell wall proteins from Coprinopsis cinerea |
| title_full | Secretome analysis in higher basidiomycetes freely secreted and cell wall proteins from Coprinopsis cinerea submitted by Dorothea Güttel |
| title_fullStr | Secretome analysis in higher basidiomycetes freely secreted and cell wall proteins from Coprinopsis cinerea submitted by Dorothea Güttel |
| title_full_unstemmed | Secretome analysis in higher basidiomycetes freely secreted and cell wall proteins from Coprinopsis cinerea submitted by Dorothea Güttel |
| title_short | Secretome analysis in higher basidiomycetes |
| title_sort | secretome analysis in higher basidiomycetes freely secreted and cell wall proteins from coprinopsis cinerea |
| title_sub | freely secreted and cell wall proteins from Coprinopsis cinerea |
| topic | Extrazellulärraum (DE-588)4205506-4 gnd Proteomanalyse (DE-588)4596545-6 gnd Sekretorische Proteine (DE-588)4728729-9 gnd Coprinus cinereus (DE-588)4391978-9 gnd |
| topic_facet | Extrazellulärraum Proteomanalyse Sekretorische Proteine Coprinus cinereus Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024499004&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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