Single molecule analysis: methods and protocols
Gespeichert in:
Weitere Verfasser: | |
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Format: | Buch |
Sprache: | English |
Veröffentlicht: |
New York ; Heidelberg [u.a.]
Humana Press
2011
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Schriftenreihe: | Methods in molecular biology
783 |
Schlagworte: | |
Online-Zugang: | Inhaltsverzeichnis |
Beschreibung: | X, 317 S. Ill., graph. Darst. |
ISBN: | 9781617792816 |
Internformat
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Datensatz im Suchindex
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adam_text | Titel: Single molecule analysis
Autor: Peterman, Erwin J. G.
Jahr: 2011
Contents
Preface..................................................................... v
Contributors................................................................ ix
1 Introduction to Optical Tweezers: Background, System Designs,
and Commercial Solutions................................................ 1
Joost van Mameren, GijsJ.L. Wuite, and Iddo Heller
2 Optical Trapping and Unfolding of RNA................................... 21
Katherine H. White and Koen Visscher
3 DNA Unzipping and Force Measurements with a Dual
Optical Trap............................................................ 45
Ismail Cissê, Pierre Mangeol, and, Ulrich Bockelmann
4 Probing the Force Generation and Stepping Behavior
of Cytoplasmic Dynein................................................... 63
Arne Gennerich and Samara L. Reek-Peterson
5 A Brief Introduction to Single-Molecule Fluorescence Methods................ 81
Siet M.J.L. van den Wildenberg, Bram Prevo, and
Erwin J. G. Peterman
6 Fluorescent Labeling of Proteins........................................... 101
Mauro Modesti
7 Fluorescence Imaging of Single Kinesin Motors
on Immobilized Microtubules............................................. 121
Till Körten, Bert Nitzsche, Chris Gell, Felix Ruhnow,
Cécile Leduc, and Stefan Diez
8 Exploring Protein Superstructures and Dynamics in Live Bacterial
Cells Using Single-Molecule and Superresolution Imaging..................... 139
Julie S. Biteen, Lucy Shapiro, and W.E. Moerner
9 Fluorescence Microscopy of Nanochannel-Confined DNA..................... 159
Fredrik Persson, Fredrik Westerlund, and Jonas O. Tegenfeldt
10 Fluorescence Correlation Spectroscopy..................................... 181
Patrick Ferrand, Jerome Wenger, and Hervé Rißneault
11 Introduction to Atomic Force Microscopy.................................. 197
Pedro J. de Pablo
12 Sample Preparation for SFM Imaging of DNA, Proteins,
and DNA-Protein Complexes............................................. 213
Dejan Ristic, Humberto Sanchez, and Claire Wyman
13 Single-Molecule Protein Unfolding and Refolding Using
Atomic Force Microscopy................................................ 233
Thomas Bornschlößl and Matthias Rief
14 How to Perform a Nanoindentation Experiment on a Virus.................... 251
Wouter H. Roos
viii Contents
15 Magnetic Tweezers for Single-Molecule Manipulation........................ 265
Teonee Seol and Keir C. Neuman
16 Probing DNA Topology Using Tethered Particle Motion...................... 295
David Dunlap, Chiara Zurla, Carlo Manzo, and Laura Finzi
Index...................................................................... 315
|
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discipline | Chemie / Pharmazie Biologie |
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isbn | 9781617792816 |
language | English |
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physical | X, 317 S. Ill., graph. Darst. |
publishDate | 2011 |
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publisher | Humana Press |
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series | Methods in molecular biology |
series2 | Methods in molecular biology Springer protocols |
spelling | Single molecule analysis methods and protocols ed. by Erwin J. G. Peterman ... New York ; Heidelberg [u.a.] Humana Press 2011 X, 317 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 783 Springer protocols Biomolekül (DE-588)4135124-1 gnd rswk-swf Fluoreszenzmikroskopie (DE-588)4290958-2 gnd rswk-swf Freies Molekül (DE-588)4489205-6 gnd rswk-swf Strukturaufklärung (DE-588)4183788-5 gnd rswk-swf Untersuchungsmethode (DE-588)4324907-3 gnd rswk-swf Biochemische Analyse (DE-588)4255721-5 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Biomolekül (DE-588)4135124-1 s Freies Molekül (DE-588)4489205-6 s Fluoreszenzmikroskopie (DE-588)4290958-2 s Strukturaufklärung (DE-588)4183788-5 s Untersuchungsmethode (DE-588)4324907-3 s DE-604 Biochemische Analyse (DE-588)4255721-5 s 1\p DE-604 Peterman, Erwin J. G. (DE-588)1141687828 edt Methods in molecular biology 783 (DE-604)BV035362695 783 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024495813&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
spellingShingle | Single molecule analysis methods and protocols Methods in molecular biology Biomolekül (DE-588)4135124-1 gnd Fluoreszenzmikroskopie (DE-588)4290958-2 gnd Freies Molekül (DE-588)4489205-6 gnd Strukturaufklärung (DE-588)4183788-5 gnd Untersuchungsmethode (DE-588)4324907-3 gnd Biochemische Analyse (DE-588)4255721-5 gnd |
subject_GND | (DE-588)4135124-1 (DE-588)4290958-2 (DE-588)4489205-6 (DE-588)4183788-5 (DE-588)4324907-3 (DE-588)4255721-5 (DE-588)4143413-4 |
title | Single molecule analysis methods and protocols |
title_auth | Single molecule analysis methods and protocols |
title_exact_search | Single molecule analysis methods and protocols |
title_full | Single molecule analysis methods and protocols ed. by Erwin J. G. Peterman ... |
title_fullStr | Single molecule analysis methods and protocols ed. by Erwin J. G. Peterman ... |
title_full_unstemmed | Single molecule analysis methods and protocols ed. by Erwin J. G. Peterman ... |
title_short | Single molecule analysis |
title_sort | single molecule analysis methods and protocols |
title_sub | methods and protocols |
topic | Biomolekül (DE-588)4135124-1 gnd Fluoreszenzmikroskopie (DE-588)4290958-2 gnd Freies Molekül (DE-588)4489205-6 gnd Strukturaufklärung (DE-588)4183788-5 gnd Untersuchungsmethode (DE-588)4324907-3 gnd Biochemische Analyse (DE-588)4255721-5 gnd |
topic_facet | Biomolekül Fluoreszenzmikroskopie Freies Molekül Strukturaufklärung Untersuchungsmethode Biochemische Analyse Aufsatzsammlung |
url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024495813&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
volume_link | (DE-604)BV035362695 |
work_keys_str_mv | AT petermanerwinjg singlemoleculeanalysismethodsandprotocols |