Human pluripotent stem cells: methods and protocols
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York ; Heidelberg [u.a.]
Humana Press
2011
|
| Schriftenreihe: | Methods in molecular biology
767 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XIX, 468 S. Ill., graph. Darst. |
| ISBN: | 9781617792007 |
Internformat
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Datensatz im Suchindex
| _version_ | 1804148500653408256 |
|---|---|
| adam_text | Titel: Human pluripotent stem cells
Autor: Schwartz, Philip H.
Jahr: 2011
Contents
Preface................................................................. v
Contributors............................................................. xv
Part I Laboratory Essentials
1 The Stem Cell Laboratory: Design, Equipment, and Oversight............... 3
Robin L. Wesselscbmidt and Philip H. Schwartz
2 Stem Cell Banks: Preserving Cell Lines, Maintaining Genetic Integrity,
and Advancing Research............................................ 15
Lyn Healy, Lesley Young, and Glyn N. Stacey
Part II Derivation
3 Embryonic Stem Cell Derivation from Human Embryos.................... 31
Paul Lerou
4 Derivation of Human Parthenogenetic Stem Cell Lines..................... 37
Nikolay Turovets, Audrey Semechkin, Leonid Kuzmichev, Jeffrey Janus,
Larissa Agapova, and Elena Revazova
5 Generation of Induced Pluripotent Stem Cell Lines from Human
Fibroblasts via Retroviral Gene Transfer................................ 55
Justine D. Miller and Thorsten M. Schlaeger
6 Derivation of Induced Pluripotent Stem Cells by Lentiviral Transduction....... 67
Hubert E. Nethercott, David J. Brick, and Philip H Schwartz
7 Transgene-Free Production of Pluripotent Stem Cells Using
pißßyBac Transposons.............................................. 87
Knut Woltjen, Riikka Hämäläinen, Mark Kibschull,
Maria Mileikovsky, and Andras Nagy
Part III Growth, Maintenance, and Expansion
8 Traditional Human Embryonic Stem Cell Culture........................ 107
Philip H. Schwartz, David J. Brick, Hubert E. Nethercott,
and Alexander E. Stover
9 Xeno-Free Culture of Human Pluripotent Stem Cells...................... 125
Rosita Bergström, Susanne Ström, Frida Holm, Anis Feki, and Outi Hovatta
10 Adaptation of Human Pluripotent Stem Cells to Feeder-Free Conditions
in Chemically Defined Medium with Enzymatic Single-Cell Passaging......... 137
Alexander E. Stover and Philip H Schwartz
xii Contents
11 GMP Scale-Up and Banking of Pluripotent Stem Cells
for Cellular Therapy Applications..................................... 147
Lara J. Ausubel, Patricia M. Lopez, and Larry A. Couture
12 Culture of Human Pluripotent Stem Cells on Glass Slides
for High-Resolution Imaging........................................ 161
Victoria Fox
Part IV Characterization
13 Classical Cytogenetics: Karyotyping Techniques.......................... 177
Steven E. Bates
14 FISH Analysis of Human Pluripotent Stem Cells.......................... 191
Suzanne E. Peterson, Jerold Chun, and Jeanne Loring
15 Immunocytochemical Analysis of Human Pluripotent Stem Cells............. 201
Hubert E. Nethercott, David J. Brick, and Philip H. Schwartz
16 Flow Cytometric Analysis of Human Pluripotent Stem Cells................. 221
Mirabelle S.H. Ho, Andrew Fryga, and Andrew L. Laslett
17 The Teratoma Assay: An In Vivo Assessment of Pluripotency................ 231
Robin L. Wesselschmidt
18 Detection of Copy Number Variation Using SNP Genotyping............... 243
Gregory M. Cooper and Heather C. Mefford
19 Genome-Wide Epigenetic Analysis of Human Pluripotent Stem Cells
by CMP and ChlP-Seq............................................. 253
Michael J. Hitchler andjudd C. Rice
20 Basic Approaches to Gene Expression Analysis of Stem Cells by Microarrays..... 269
Bernhard Schuldt, Qiong Lin, Franz-Josef Müller, and Jeanne Loring
21 Human Pluripotent Stem Cells: The Development of High-Content
Screening Strategies............................................... 283
Sean P. Sherman, Jackelyn A. Alva, Kaushali Thakore-Shah,
and April D. Pyle
22 Quantitative Proteome and Phosphoproteome Analysis of Human
Pluripotent Stem Cells............................................. 297
Javier Munoz and Albert J.R. Heck
Part V Genetic Manipulation (Gene Modulation)
23 Lentivirus-Mediated Modification of Pluripotent Stem Cells................. 315
Ruchi Bajpai
24 Nucleofection of Human Embryonic Stem Cells.......................... 333
Helen Fong, K.A. Hohenstein Elliott, Leslie F. Lock, and Peter J. Donovan
25 Nonviral Gene Delivery in Neural Progenitors Derived from Human
Pluripotent Stem Cells............................................. 343
Sujoy K. Dhara, Anirban Majumder, Mahesh C. Dodla, and Steven L. Stice
26 Gene Targeting in Human Pluripotent Stem Cells......................... 355
Ting Liu and Mahendra Rao
27 Episomal Transgene Expression in Pluripotent Stem Cells................... 369
Michèle M.P. Lufino, Anna R. Popplestone, Sally A. Cowley,
Pauline A.H. Edser, William S. James, and Richard Wade-Martins
Contents xiii
Part VT Differentiation
28 The Generation of Embryoid Bodies from Feeder-Based or Feeder-Free
Human Pluripotent Stem Cell Cultures................................. 391
Alexander E. Stover and Philip H Schwartz
29 Derivation of Oligodendrocyte Progenitor Cells from Human
Embryonic Stem Cells............................................. 399
Jason Sharp, Maya Hatch, Gabriel Nistor, and Hans Keirstead
30 Directed Differentiation of Dopamine Neurons from Human
Pluripotent Stem Cells............................................. 411
Lixiang Ma, Tan Liu, and Su-Chun Zhang
31 Methods for the Derivation and Use of Cardiomyocytes from Human
Pluripotent Stem Cells............................................. 419
Wei-Zhong Zhu, Benjamin Van Biber, and Michael A. Laflamme
32 In Vivo Evaluation of Putative Hematopoietic Stem Cells Derived
from Human Pluripotent Stem Cells................................... 433
Melinda K. Hexum, Xinghui Tian, and Dan S. Kaufman
33 Differentiation of Dendritic Cells from Human Embryonic Stem Cells......... 449
Kathryn M. Silk, Su-Ti Tseng, Kevin P. Nishimoto, Jane Lebkowski,
Anita Reddy, and Paul J. Fairchild
Index.................................................................. 463
|
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| isbn | 9781617792007 |
| language | English |
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| physical | XIX, 468 S. Ill., graph. Darst. |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | Human pluripotent stem cells methods and protocols ed. by Philip H. Schwartz ... New York ; Heidelberg [u.a.] Humana Press 2011 XIX, 468 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 767 Springer protocols Stammzelle (DE-588)4665329-6 gnd rswk-swf Pluripotenz (DE-588)4420540-5 gnd rswk-swf Stammzelle (DE-588)4665329-6 s Pluripotenz (DE-588)4420540-5 s DE-604 Schwartz, Philip H. edt Erscheint auch als Online-Ausgabe Human Pluripotent Stem Cells Methods in molecular biology 767 (DE-604)BV035362695 767 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024494093&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
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| subject_GND | (DE-588)4665329-6 (DE-588)4420540-5 |
| title | Human pluripotent stem cells methods and protocols |
| title_auth | Human pluripotent stem cells methods and protocols |
| title_exact_search | Human pluripotent stem cells methods and protocols |
| title_full | Human pluripotent stem cells methods and protocols ed. by Philip H. Schwartz ... |
| title_fullStr | Human pluripotent stem cells methods and protocols ed. by Philip H. Schwartz ... |
| title_full_unstemmed | Human pluripotent stem cells methods and protocols ed. by Philip H. Schwartz ... |
| title_short | Human pluripotent stem cells |
| title_sort | human pluripotent stem cells methods and protocols |
| title_sub | methods and protocols |
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| topic_facet | Stammzelle Pluripotenz |
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