Biology of serpins:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Amsterdam [u.a.]
Elsevier, Acad. Press
2011
|
| Ausgabe: | 1. ed. |
| Schriftenreihe: | Methods in enzymology
499 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | L, 405 S. Ill., graph. Darst. |
| ISBN: | 9780123864710 |
Internformat
MARC
| LEADER | 00000nam a2200000 cb4500 | ||
|---|---|---|---|
| 001 | BV039587493 | ||
| 003 | DE-604 | ||
| 005 | 20111021 | ||
| 007 | t | ||
| 008 | 110919s2011 ad|| |||| 00||| eng d | ||
| 020 | |a 9780123864710 |9 978-0-12-386471-0 | ||
| 035 | |a (OCoLC)752356935 | ||
| 035 | |a (DE-599)BVBBV039587493 | ||
| 040 | |a DE-604 |b ger |e rakwb | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-355 |a DE-19 |a DE-12 |a DE-91G | ||
| 084 | |a VK 8700 |0 (DE-625)147548:253 |2 rvk | ||
| 084 | |a WC 4355 |0 (DE-625)148096: |2 rvk | ||
| 084 | |a CHE 820f |2 stub | ||
| 084 | |a CHE 832f |2 stub | ||
| 245 | 1 | 0 | |a Biology of serpins |c ed. by James C. Whisstock ... |
| 250 | |a 1. ed. | ||
| 264 | 1 | |a Amsterdam [u.a.] |b Elsevier, Acad. Press |c 2011 | |
| 300 | |a L, 405 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in enzymology |v 499 | |
| 650 | 0 | 7 | |a Serpine |0 (DE-588)4285093-9 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Methode |0 (DE-588)4038971-6 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4143413-4 |a Aufsatzsammlung |2 gnd-content | |
| 689 | 0 | 0 | |a Serpine |0 (DE-588)4285093-9 |D s |
| 689 | 0 | 1 | |a Methode |0 (DE-588)4038971-6 |D s |
| 689 | 0 | |C b |5 DE-604 | |
| 700 | 1 | |a Whisstock, James C. |e Sonstige |4 oth | |
| 830 | 0 | |a Methods in enzymology |v 499 |w (DE-604)BV000000938 |9 499 | |
| 856 | 4 | 2 | |m Digitalisierung UB Regensburg |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024438656&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-024438656 | ||
Datensatz im Suchindex
| _version_ | 1804148421381062656 |
|---|---|
| adam_text | Contents
Contributors
xi
Preface
xix
Volumes in Series
xxi
1.
Analysis of Serpin Secretion, Misfolding, and Surveillance
in the Endoplasmic Reticulum
1
Shujuan Pan, Michael J. lannotti, and Richard
N.
Sifers
1.
Introduction
2
2.
Protocols
3
3.
Concluding Remarks
14
Acknowledgments
14
References
14
2.
Serpm-Enzyme Receptors: LDL Receptor-Related Protein
1 17
Dudley K. Strickland,
Selen
Catania Muratoglu, and
Toni
M. Antalis
1.
Introduction
18
2.
Purification of LRP1
18
3.
Expression of Receptor Fragments
21
4.
Ugand
Binding to LRP1
23
5.
Binding of Serpin-Enzyme Complexes to LRP1
26
6.
Summary
28
Acknowledgments
28
References
28
3.
The Role of Autophagy in Alpha-l-Antrtrypsin Deficiency
33
Tunda
Hidvegi, Amitava Mukherjee, Michael Ewing, Carolyn Kemp,
and David H.
Perlmutter
1.
Introduction
34
2.
Methods for Detection of Autophagy in Cell Line Models
36
3.
LC3 Conversion Assay
38
4.
Assessing Autophagy by Analyzing Degradation
of Long-Lived Proteins
41
5.
Use of Autophagy-Deflcient Cell Lines
42
vi
Contents
6.
Pulse-Chase Labeling to Determine the Effect of Autophagy
on Degradation of Individual Proteins
43
7.
Methods for Detection of Hepatic Autophagy In Vivo
45
8.
Methods for Detection of Hepatic Injury
48
References
52
4.
Serpins and the Complement System
55
László
Beinrohr,
Thomas A. Murray-Rust, Leanne Dyksterhuis,
Péter
Závodszky, Péter Gál,
Robert
N.
Pike, and Lakshmi
C. Wijeyewickrema
1.
introduction
56
2.
Human Plasma Cl-lnhibitor
57
3.
Production of
Recombinant
Cl-lnhibitor in Yeast
64
Acknowledgments
73
References
74
5.
Use of Mouse Models to Study Plasminogen Activator Inhibitor-
1 77
Paul J. Declerck, Ann Gils, and
Bart De Taeye
1.
Introduction
78
2.
Role of PAI-1 in Different (Patho) Physiological Processes
80
3.
Mouse Models to Study the Role of PAI-1
82
4.
Mouse (Wild-Type) Models to Study the Role of PAI-1
in Cardiovascular Disease and Cancer
83
5.
Genetically Modified Mice to Study the Role of PAI-1
in Cardiovascular Disease and Cancer
87
6.
Conclusions
93
References
94
6.
Plasminogen Activator Inhibitor Type
2:
Still an Enigmatic Serpin but a Model for Gene Regulation
105
Robert L. Medcalf
1.
Introduction
106
2.
PAI-2 and the Plasminogen-Activating System
107
3.
General Features of PAI-2
108
4.
PAI-2 Gene Expression and Regulation
115
5.
Conclusions
122
6.
Methodology: Rapid Run-On Transcription Assay Protocol
123
Acknowledgments
126
References
126
Contents
Vil
7.
The SerpinBl Knockout Mouse: A Model for Studying
Neutrophil Protease Regulation in Homeostasis
and Inflammation
135
Charaf Benarafa
1.
Generation of SerpinBl Knockout Mice
136
2.
Models of Lung Infection and Inflammation
139
3.
Neutrophil Homeostasis
142
Acknowledgments
146
References
146
8.
Investigating Maspin in Breast Cancer Progression
Using Mouse Models
149
Michael P. Endsley and Ming Zhang
1.
Introduction
150
2.
Transgenic Mouse Models
151
3.
Syngeneic Tumor Model
157
4.
Conclusion
161
References
162
9.
Hsp47 as a Collagen-Specific Molecular Chaperone
167
Yoshihito Ishida and Kazuhiro Nagata
1.
Introduction
168
2.
Hsp47 as a Collagen-Binding Protein in the
ER 169
3.
Interaction and Recognition of Collagen by Hsp47
170
4.
A Phenotype and Abnormal Collagen Maturation in
Hsp47 Knockout Mice
171
5.
Possible Roles of Hsp47 in
Procollagen
Maturation
in the
ER 172
6.
Hsp47 Null Cells: A Tool for Studying the Fate of Misfolded Collagen
174
7.
Regulation of Hsp47 Expression and Its
Clinicai
Importance
176
References
178
10.
Assays for the Antiangiogenic and Neurotrophic
Serpin Pigment Epithelium-Derived Factor
183
Preeti Subramanian, Susan E. Crawford, and S. Patricia
Becerra
1.
Introduction
184
2.
Purification of PEDF Protein
185
3.
Techniques to Assay PEDF
188
4.
Neurotrophic Assays
194
5.
Antiangiogenic Assays
197
Acknowledgments
202
References
202
v¡¡¡
Contents
11.
The Drosophila
Serpins: Multiple
Functions
in
Immunity and Morphogenesis
205
Jean
Marc Reichhart, David Gubb, and Vincent Ledere
1.
Introduction
206
2. The Range
of
Drosophila
Serpin
Functions
207
3.
Techniques for Analysis of Immune Response
214
Acknowledgments
220
References
220
12.
Modeling Serpin Conformational Diseases
in
Drosophila melanogaster
227
Thomas R.
Jahn, Elke Malzer,
John Roote,
Anastasia
Vishnivetskaya, Sara Imarisio, Maria Giannakou,
Karin
Panser,
Stefan Marciniak, and
Damian
C. Crowther
1.
Introduction
228
2.
Why
Drosophila
Models of Serpinopathies?
229
3.
Screening for Polymerogenic Mutations in Physiologically
Important Fly Serpins
229
4.
First Steps in the Generation of a Human Serpinopathy
Model in
Drosophila
230
5.
Longevity Assays in Flies Expressing Human Serpins
233
6.
Behavioral Assays in Flies Expressing Human Serpins
234
7.
Microscopic Phenotyping of Flies Expressing Human Serpins
235
8.
Using Genetic Screens to Identify Genes That Have a Role in
Generating, or Suppressing Serpin-lnduced Phenotypes
236
9.
Conclusions
237
10.
Method
1:
Generating Transgenic
Drosophila
237
11.
Method
2:
The
Drosophila UAS/GAL4
Expression System
242
12.
Method
3:
Chemical Mutagenesis and X-ray Mutagenesis
242
13.
Method
4:
Screening for Mutations Caused
by Chemical and X-ray Mutagenesis
245
14.
Method
6:
Examination of the Eye
Imaginai Disc
246
15.
Method
7:
Pseudopupil Assay
248
16.
Method
8:
Protein Extraction from Flies
250
17.
Method
9:
Genetic Backcrossing
251
18.
Method
10:
Longevity Assays
252
19.
Method
11:
Locomotor
Assays
253
20.
Method
12:
Immunostaining of Fly Brains
253
21.
Method
13:
P-element and RNAi Screening
254
22.
Method
15:
Deletion Kit Screening
255
References
255
Contents ix
13.
Using
Caenorhabditis
elegans
to Study Serpinopathies
259
Olivia S. Long, Sager J. Gosai,
Joon
Hyeok
Kwak,
Dale E. King,
David H.
Perlmutter,
Gary
A. Silverman,
and Stephen
С. Рак
1.
Introduction
260
2.
Considerations for
Transgenesis 261
3.
Microinjection
268
4.
High-Content Drug Screening
273
Acknowledgments
279
References
279
14.
Using
C. elegans
to Identify the Protease
Targets of Serpins In Vivo
283
Sangeeta R. Bhatia, Mark T. Miedel,
Cavita
К.
Chotoo, Nathan J. Graf,
Brian L. Hood, Thomas P.
Conrads,
Gary
A. Silverman,
and Cliff J. Luke
1.
Introduction
284
2.
Methods for Identifying the Targets of Intracellular
Serpins in
C. elegans
286
Acknowledgments
297
References
297
15.
Viral Serpin Therapeutics: From Concept to Clinic
301
Hao Chen, Donghang Zheng, Jennifer Davids,
Mee Yong
Bartee,
Erbin
Dai, Liying Liu, Lyubomir
Petrov,
Colin Macaulay, Robert Thoburn,
Eric
Sobel,
Richard Moyer, Grant McFadden, and Alexandra Lucas
1.
Introduction
302
2.
From Viral Pathogenesis to Identification of Immunomodulatory
Potential
305
3.
Testing Biological Potential
307
4.
Assessing Clinical Therapeutic Potential for a Viral Serpin
in Clinical Trial: Trial of Serp-1 Treatment in Patients with
Acute Coronary Syndrome and Stent Implant
319
References
326
16.
Human SCCA Serpins Inhibit Staphyiococcal
Cysteine
Proteases by Forming Classic Serpin-Like
Covalent Complexes
331
Tomasz Kantyka
and Jan Potempa
1.
Introduction
332
2.
Purification of Staphopains
333
3.
Purification of GST-SCCAl and GST-SCCA2 Fusion Proteins
333
4.
Characterization of Inhibition
334
χ
Contents
5.
Detection of
Serpin-Enzyme
Complex
338
6.
Determination of an Interaction Site
341
7.
Summary
343
Acknowledgments
344
References
344
17.
Plants and the Study of Serpin Biology
347
Thomas H. Roberts,
Joon-Woo Ahn,
Nardy
Lampi,
and Robert Fluhr
1.
Introduction
348
2.
Detection of Serpins in Plant Extracts and Localization
of Serpins in Plant Tissues and Cells
349
3.
Purification of Serpins from Plant Tissues
353
4.
Production of
Recombinant
Plant Serpins
360
5.
Analysis of Plant Serpin-Protease Interactions
360
Acknowledgments
364
References
364
Author Index
369
Subject Index
397
|
| any_adam_object | 1 |
| building | Verbundindex |
| bvnumber | BV039587493 |
| classification_rvk | VK 8700 WC 4355 |
| classification_tum | CHE 820f CHE 832f |
| ctrlnum | (OCoLC)752356935 (DE-599)BVBBV039587493 |
| discipline | Chemie / Pharmazie Biologie Chemie |
| edition | 1. ed. |
| format | Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01602nam a2200421 cb4500</leader><controlfield tag="001">BV039587493</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20111021 </controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">110919s2011 ad|| |||| 00||| eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9780123864710</subfield><subfield code="9">978-0-12-386471-0</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)752356935</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV039587493</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield><subfield code="a">DE-19</subfield><subfield code="a">DE-12</subfield><subfield code="a">DE-91G</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">VK 8700</subfield><subfield code="0">(DE-625)147548:253</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 4355</subfield><subfield code="0">(DE-625)148096:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">CHE 820f</subfield><subfield code="2">stub</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">CHE 832f</subfield><subfield code="2">stub</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Biology of serpins</subfield><subfield code="c">ed. by James C. Whisstock ...</subfield></datafield><datafield tag="250" ind1=" " ind2=" "><subfield code="a">1. ed.</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Amsterdam [u.a.]</subfield><subfield code="b">Elsevier, Acad. Press</subfield><subfield code="c">2011</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">L, 405 S.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="1" ind2=" "><subfield code="a">Methods in enzymology</subfield><subfield code="v">499</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Serpine</subfield><subfield code="0">(DE-588)4285093-9</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Methode</subfield><subfield code="0">(DE-588)4038971-6</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4143413-4</subfield><subfield code="a">Aufsatzsammlung</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Serpine</subfield><subfield code="0">(DE-588)4285093-9</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Methode</subfield><subfield code="0">(DE-588)4038971-6</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="C">b</subfield><subfield code="5">DE-604</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Whisstock, James C.</subfield><subfield code="e">Sonstige</subfield><subfield code="4">oth</subfield></datafield><datafield tag="830" ind1=" " ind2="0"><subfield code="a">Methods in enzymology</subfield><subfield code="v">499</subfield><subfield code="w">(DE-604)BV000000938</subfield><subfield code="9">499</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">Digitalisierung UB Regensburg</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024438656&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-024438656</subfield></datafield></record></collection> |
| genre | (DE-588)4143413-4 Aufsatzsammlung gnd-content |
| genre_facet | Aufsatzsammlung |
| id | DE-604.BV039587493 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T00:06:52Z |
| institution | BVB |
| isbn | 9780123864710 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-024438656 |
| oclc_num | 752356935 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-19 DE-BY-UBM DE-12 DE-91G DE-BY-TUM |
| owner_facet | DE-355 DE-BY-UBR DE-19 DE-BY-UBM DE-12 DE-91G DE-BY-TUM |
| physical | L, 405 S. Ill., graph. Darst. |
| publishDate | 2011 |
| publishDateSearch | 2011 |
| publishDateSort | 2011 |
| publisher | Elsevier, Acad. Press |
| record_format | marc |
| series | Methods in enzymology |
| series2 | Methods in enzymology |
| spelling | Biology of serpins ed. by James C. Whisstock ... 1. ed. Amsterdam [u.a.] Elsevier, Acad. Press 2011 L, 405 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in enzymology 499 Serpine (DE-588)4285093-9 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Serpine (DE-588)4285093-9 s Methode (DE-588)4038971-6 s b DE-604 Whisstock, James C. Sonstige oth Methods in enzymology 499 (DE-604)BV000000938 499 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024438656&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Biology of serpins Methods in enzymology Serpine (DE-588)4285093-9 gnd Methode (DE-588)4038971-6 gnd |
| subject_GND | (DE-588)4285093-9 (DE-588)4038971-6 (DE-588)4143413-4 |
| title | Biology of serpins |
| title_auth | Biology of serpins |
| title_exact_search | Biology of serpins |
| title_full | Biology of serpins ed. by James C. Whisstock ... |
| title_fullStr | Biology of serpins ed. by James C. Whisstock ... |
| title_full_unstemmed | Biology of serpins ed. by James C. Whisstock ... |
| title_short | Biology of serpins |
| title_sort | biology of serpins |
| topic | Serpine (DE-588)4285093-9 gnd Methode (DE-588)4038971-6 gnd |
| topic_facet | Serpine Methode Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024438656&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV000000938 |
| work_keys_str_mv | AT whisstockjamesc biologyofserpins |