Plant kinases: methods and protocols
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York
Humana Press
2011
|
| Schriftenreihe: | Methods in molecular biology
779 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 285 S. Ill., graph. Darst. |
| ISBN: | 9781617792632 |
Internformat
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Datensatz im Suchindex
| _version_ | 1804148385877327872 |
|---|---|
| adam_text | Plant Kinases
Methods and Protocols
Edited by
Nico Dissmeyer
Leibniz Institute of Plant Biochemistry (IPB), Halle (Saale), Germany
Arp Schnittger
Institut de Biologie Moleculaire des Plantes
Centre National de la Recherche Scientifique (IBMP-CNRS), Strasbourg, France
$),£ Humana Press
Contents
Preface v
Contributors ix
PART I OVERVIEW
1 Guide to the Book Plant Kinases 3
Nico Dissmeyer and Arp Schnittger
2 The Age of Protein Kinases 7
Nico Dissmeyer and Arp Schnittger
PART II KINASE EXPRESSION, PURIFICATION AND ACTIVITY ASSAYS
3 Expression and Purification of Active Protein Kinases
from Wheat Germ Extracts 55
Bogldrka Sonkoly, Viola Barddczy, and Tamds Meszdros
4 Measurement of Plant Cyclin-Dependent Kinase Activity Using
Immunoprecipitation-Coupled and Affinity Purification-Based
Kinase Assays and the Baculovirus Expression System 65
Hirofumi Harashima and Masami Sekine
5 Mitogen-Activated Protein Kinase Activity and Reporter
Gene Assays in Plants 79
Rdbert Doczi, Elizabeth Hatzimasoura, and Laszld Bo gre
6 Use of Phospho-Site Substitutions to Analyze the Biological
Relevance of Phosphorylation Events in Regulatory Networks 93
Nico Dissmeyer and Arp Schnittger
7 Bacterial Assay to Study Plant Sensor Histidine Kinases 139
Lukds Spichal
8 Substrate Analysis of Arabidopsis ¥P2C-Tyipc Protein Phosphatases 149
Julija Umbrasaite, Alois Schweighofer, and Irute Meskiene
PART III STUDYING KINASE FUNCTION AT THE CELLULAR LEVEL
9 Modulating and Monitoring MAPK Activity During Programmed
Cell Death in Pollen 165
Sbutian Li and Vernonica E Franklin-Tong
10 Sensitizing Plant Protein Kinases to Specific Inhibition
by ATP-Competitive Molecules 185
Dor Salomon, Chao Zhang, Kevan M Shokat, and Guido Sessa
11 Fluorescence Fluctuation Analysis of Receptor Kinase Dimerization 199
Mark A Hink, Sacco C de Vries, and Antonie JWG Visser
VII
viii Contents
12 Quantifying Degradation Rates of Transmembrane Receptor Kinases 217
Niko Geldner
13 Fluorescence Correlation Spectroscopy and Fluorescence Recovery
After Photobleaching to Study Receptor Kinase Mobility In Planta 225
Mark Kwaaitaal, Marieke Schor, Mark A Hink,
AntonieJ W G Visser, and Sacco C de Vries
PART IV IDENTIFICATION OF KINASE SUBSTRATES
AND PHOSPHORYLATION-SlTE DETECTION
14 Bimolecular-Fluorescence Complementation Assay to Monitor
Kinase-Substrate Interactions In Vivo 245
Stefan Pusch, Nico Dissmeyer, and Arp Schnittger
15 Chemical Genetic Analysis of Protein Kinase Function in Plants 259
Maik Bdhmer, Michael Bb lker, and Tina Romeis
16 Modified Metal-Oxide Affinity Enrichment Combined with 2D-PAGE
and Analysis of Phosphoproteomes 273
Thomas Colby, Horst Rb hrig, Anne Harzen, and Jiirgen Schmidt
17 Phosphoproteomics Using iTRAQ 287
Alexandra M E Jones and Thomas S Ntihse
Index : 303
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| title | Plant kinases methods and protocols |
| title_auth | Plant kinases methods and protocols |
| title_exact_search | Plant kinases methods and protocols |
| title_full | Plant kinases methods and protocols ed. by Nico Dissmeyer ... |
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| title_short | Plant kinases |
| title_sort | plant kinases methods and protocols |
| title_sub | methods and protocols |
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