Arabidopsis flavonoid glycosylation impacts on phenylpropanoid biosynthesis and plant growth:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2010
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| Schlagworte: | |
| Online-Zugang: | Volltext https://nbn-resolving.org/urn:nbn:de:bvb:19-131462 Inhaltsverzeichnis |
| Beschreibung: | VII, 94 S. Ill., graph. Darst. |
Internformat
MARC
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| 100 | 1 | |a Yin, Ruohe |d 1977- |e Verfasser |0 (DE-588)1012966569 |4 aut | |
| 245 | 1 | 0 | |a Arabidopsis flavonoid glycosylation impacts on phenylpropanoid biosynthesis and plant growth |c Ruohe Yin |
| 264 | 1 | |c 2010 | |
| 300 | |a VII, 94 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a München, Univ., Diss., 2010 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
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| 912 | |a ebook | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-024410635 | ||
Datensatz im Suchindex
| _version_ | 1804148382640373760 |
|---|---|
| adam_text | IMAGE 1
CONTENT IV
SUMMARY I
CURRICULUM VITAE ILL
1 CHAPTER 1: GENERAL INTRODUCTION 8
1.1 GLYCOSYLTRANSFERASE 8
1.2 FLAVONOID BIOSYNTHESIS IN ARABIDOPSIS 9
1.3 FLAVONOID GLYCOSYLTRANSFERASES IN ARABIDOPSIS 12
1.4 TRANSCRIPTIONAL REGULATION OF FLAVONOL BIOSYNTHESIS 13
1.5 ENZYME COMPLEXES IN THE PHENYLPROPANOID AND FLAVONOID PATHWAY 13
1.6 THE INFLUENCE OF FLAVONOIDS ON PLANT GROWTH AND DEVELOPMENT 14
1.7 FLAVONOIDS AND AUXIN TRANSPORT 15
1.8 AIMS OF THE THESIS 17
2 CHAPTER 2: MATERIALS AND METHODS 18
2.1 MATERIALS 18
2.1.1 PLANT MATERIAL 18
2.1.2 BUFFERS AND MEDIA 18
2.1.3 VECTORS 18
2.1.4 RESTRICTION ENDONUCLEASES AND DNA MODIFYING ENZYMES 19
2.1.5 OLIGONUCLEOTIDES AND SEQUENCING 19
2.2 METHODS 19
2.2.1 PLANT GROWTH CONDITIONS 19
2.2.2 SEED SURFACE STERILIZATION 20
2.2.3 BACTERIAL STRAINS 20
2.2.4 PREPARATION OF E. COLI COMPETENT CELLS 20
2.2.5 E. COLI TRANSFORMATION BY HEAT SHOCK 20
2.2.6 AGROBACTERIUM TRANSFORMATION BY ELECTROPORATION 21
2.2.7 ARABIDOPSIS TRANSFORMATION BY FLORAL DIP 21
2.2.8 SELECTION OF TRANSFORMED ARABIDOPSIS PLANTS 21
2.2.9 HISTOCHEMICAL GUS ASSAY 21
2.2.10 PREPARATION OF PLANT GENOMIC DNA 22
2.2.11 PREPARATION OF PLASMID DNA 22
2.2.12 ISOLATION OF TOTAL RNA FOR RT-PCR 22
HTTP://D-NB.INFO/104191296X
IMAGE 2
CONTENT
V
2.2.13 DETERMINATION OF NUCLEIC ACID CONCENTRATIONS 23
2.2.14 GEL ELECTROPHORESIS 23
2.2.15 PURIFICATION OF PCR PRODUCTS 23
2.2.16 DNA GEL EXTRACTION 23
2.2.17 REAL-TIME QUANTITATIVE RT-PCR 23
2.2.18 MOLECULAR CLONING USING THE GATEWAY TECHNOLOGY 24
2.2.19 BIOINFORMATICS TOOL 24
2.2.20 CONSTRUCT FOR UGF78D1 MUTANT GENETIC COMPLEMENTATION 24
2.2.21 CONSTRUCT FOR UGRF78D2 MUTANT GENETIC COMPLEMENTATION 25
2.2.22 CONSTRUCT FOR ARTIFICIAL MIRNA 25
2.2.23 EXTRACTION AND DETERMINATION OF FREE AND GLUCOSE-CONJUGATED SA 26
2.2.24 PAL ACTIVITY ASSAY 26
2.2.25 CONSTRUCTION OF THE CALIBRATION CURVES FOR DIFFERENT FLAVONOLS 26
2.2.26 EXTRACTION OF FLAVONOL METABOLITES FOR HPLC ANALYSIS 28
2.2.27 HPLC ANALYSIS OF FLAVONOLS AND SINAPATE ESTERS 28
2.2.28 PHOTOMETRIC DETERMINATION OF ANTHOCYANINS 29
2.2.29 LC-ESI-MS 29
2.2.30 LC-ESI-MS METABOLITE ANALYSIS 29
2.2.31 AUXIN TRANSPORT ASSAY 30
2.2.32 IAA QUANTIFICATION 30
2.2.33 IMAGING METHODS 30
3 CHAPTER 3: FEEDBACK INHIBITION OF PHENYLPROPANOID AND
FLAVONOL-BIOSYNTHETIC PATHWAYS UPON A COMPROMISED
FLAVONOL3-O-GLYCOSYLATION 31
3.1 ABSTRACT 31
3.2 INTRODUCTION 31
3.3 RESULTS 33
3.3.1 THE UGT78D1 UGT78D2 DOUBLE MUTANT HAS STRONGLY REDUCED TOTAL
FLAVONOL
CONTENT IN LEAVES 33
3.3.2 EXPRESSION PATTERN OF UGT78D1 AND UGT78D2 MATCHES ORGAN-SPECIFIC
REDUCTION IN FLAVONOLS 37
3.3.3 FLAVONOID BIOSYNTHETIC GENES ARE TRANSCRIPTIONALLY DOWN-REGULATED
IN
UGT78D1 UGT78D2 DOUBLE MUTANT LEAVES 39
IMAGE 3
CONTENT VI
3.3.4 PAL ENZYME ACTIVITY IS REDUCED IN THE UGT78D1 UGT78D2 DOUBLE
MUTANT
40
3.3.5 THE INFLUENCE OF REDUCED PAL ACTIVITY ON PHENYLPROPANOID
METABOLISM
41
3.3.6 THE REDUCTION OF PAL EXPRESSION OF UGT78D1 UGT78D2 IS DEPENDENT ON
FLAVONOL AGLYCONE FORMATION 44
3.4 DISCUSSION 48
4 CHAPTER 4: THE OVER-ACCUMULATION OF KAEMPFEROL 3-0-
RHAMNOSIDE-7-O-RHAMNOSIDE IN THE UGT78D2 MUTANT RESULTS IN
DECREASED BASIPETAL AUXIN TRANSPORT AND PLANT GROWTH
DEFECTS 52
4.1 ABSTRACT 52
4.2 INTRODUCTION 52
4.3 RESULTS 55
4.3.1 HEIGHT AND STATURE OF UGT78D2 PLANTS ARE ALTERED 55
4.3.2 THE UGT78D2 GROWTH DEFECTS ARE FLAVONOID-DEPENDENT 57
4.3.3 KAEMPFEROL 3-0-RHAMNOSIDE-7-0-RHAMNOSIDE IS THE UGT78D2
PHENOTYPE-INDUCING FLAVONOL 57
4.3.4 ANTHOCYANINS ARE NOT RESPONSIBLE FOR THE UGT78D2 GROWTH DEFECTS 61
4.3.5 BASIPETAL AUXIN TRANSPORT IS REDUCED IN INFLORESCENCE STEM OF
UGT78D263
4.3.6 FREE IAA QUANTIFICATION IN WT AND UGT78D2 PLANTS 66
4.3.7 EXPRESSION PROFILE OF UGT78D1 POINTS TO K1 AS AN ENDOGENOUS AUXIN
TRANSPORT INHIBITOR 66
4.4 DISCUSSION 68
4.4.1 THE ROLE OF K1 IN REPRESSING PLANT GROWTH 68
4.4.2 KAEMPFEROL 3-0-RHAMNOSIDE-7-0-RHAMNOSIDE IS AN ENDOGENOUS AUXIN
TRANSPORT INHIBITOR 70
4.4.3 ARE FLAVONOL AGLYCONES INVOLVED IN THE INHIBITION OF AUXIN
TRANSPORT IN
UGT78D2 ? 71
4.4.4 POSSIBLE MECHANISMS HOW FLAVONOLS MODULATE AUXIN TRANSPORT 71
5 CHAPTER 5: ARABIDOPSIS UGT74F1 AND UGT74F2 ARE NOT
RESPONSIBLE FOR STRESS-RELATED SALICYLIC ACID GLUCOSYLATION 73
5.1 INTRODUCTION 73
IMAGE 4
CONTENT VII
5.2 RESULTS 73
5.2.1 UGT74F2 IS EFFICIENTLY SILENCED BY THE AMIRNA 73
5.2.2 THE UGT74F1 UGT74F2 DOUBLE MUTANT HAS WILD-TYPE SA LEVELS 75
5.3 DISCUSSION 77
6 REFERENCES 78
7 SUPPPLEMENTAL DATA 91
ABBREVIATIONS 92
ACKNOWLEDGEMENTS 93
|
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| language | English |
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| physical | VII, 94 S. Ill., graph. Darst. |
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| spelling | Yin, Ruohe 1977- Verfasser (DE-588)1012966569 aut Arabidopsis flavonoid glycosylation impacts on phenylpropanoid biosynthesis and plant growth Ruohe Yin 2010 VII, 94 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier München, Univ., Diss., 2010 (DE-588)4113937-9 Hochschulschrift gnd-content Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:19-131462 http://edoc.ub.uni-muenchen.de/13146/ Verlag kostenfrei Volltext https://nbn-resolving.org/urn:nbn:de:bvb:19-131462 Resolving-System DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024410635&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Yin, Ruohe 1977- Arabidopsis flavonoid glycosylation impacts on phenylpropanoid biosynthesis and plant growth |
| subject_GND | (DE-588)4113937-9 |
| title | Arabidopsis flavonoid glycosylation impacts on phenylpropanoid biosynthesis and plant growth |
| title_auth | Arabidopsis flavonoid glycosylation impacts on phenylpropanoid biosynthesis and plant growth |
| title_exact_search | Arabidopsis flavonoid glycosylation impacts on phenylpropanoid biosynthesis and plant growth |
| title_full | Arabidopsis flavonoid glycosylation impacts on phenylpropanoid biosynthesis and plant growth Ruohe Yin |
| title_fullStr | Arabidopsis flavonoid glycosylation impacts on phenylpropanoid biosynthesis and plant growth Ruohe Yin |
| title_full_unstemmed | Arabidopsis flavonoid glycosylation impacts on phenylpropanoid biosynthesis and plant growth Ruohe Yin |
| title_short | Arabidopsis flavonoid glycosylation impacts on phenylpropanoid biosynthesis and plant growth |
| title_sort | arabidopsis flavonoid glycosylation impacts on phenylpropanoid biosynthesis and plant growth |
| topic_facet | Hochschulschrift |
| url | http://edoc.ub.uni-muenchen.de/13146/ https://nbn-resolving.org/urn:nbn:de:bvb:19-131462 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024410635&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT yinruohe arabidopsisflavonoidglycosylationimpactsonphenylpropanoidbiosynthesisandplantgrowth |