Long term observation of living cells by wide field fluorescence lifetime imaging microscopy:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Berlin
mbv, Mensch-und-Buch-Verl.
2011
|
| Schriftenreihe: | Chemical science
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 128 S. Ill., graph. Darst. |
| ISBN: | 9783866649897 |
Internformat
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV039519705 | ||
| 003 | DE-604 | ||
| 005 | 00000000000000.0 | ||
| 007 | t | ||
| 008 | 110809s2011 ad|| m||| 00||| eng d | ||
| 020 | |a 9783866649897 |9 978-3-86664-989-7 | ||
| 035 | |a (OCoLC)794533247 | ||
| 035 | |a (DE-599)BVBBV039519705 | ||
| 040 | |a DE-604 |b ger |e rakwb | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-83 | ||
| 082 | 0 | |a 571.60282 |2 22/ger | |
| 100 | 1 | |a Vitali, Marco |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Long term observation of living cells by wide field fluorescence lifetime imaging microscopy |c vorgelegt von Marco Vitali |
| 246 | 1 | |a Long-term observation of living cells by wide-field fluorescence lifetime imaging microscopy | |
| 264 | 1 | |a Berlin |b mbv, Mensch-und-Buch-Verl. |c 2011 | |
| 300 | |a 128 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 0 | |a Chemical science | |
| 502 | |a Zugl.: Berlin, Techn. Univ., Diss., 2011 | ||
| 650 | 0 | 7 | |a Fluoreszenz-Resonanz-Energie-Transfer |0 (DE-588)4773018-3 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Fluoreszenzmikroskop |0 (DE-588)4328299-4 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Zelle |0 (DE-588)4067537-3 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Zelle |0 (DE-588)4067537-3 |D s |
| 689 | 0 | 1 | |a Fluoreszenzmikroskop |0 (DE-588)4328299-4 |D s |
| 689 | 0 | 2 | |a Fluoreszenz-Resonanz-Energie-Transfer |0 (DE-588)4773018-3 |D s |
| 689 | 0 | |5 DE-604 | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024372225&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-024372225 | ||
Datensatz im Suchindex
| _version_ | 1804148327150780416 |
|---|---|
| adam_text | IMAGE 1
CONTENTS
CONTENTS 1
DEUTSCHE ZUSAMMENFASSUNG 3
GENERAL INTRODUCTION 5
1 WIDE-FIELD MULTI-PARAMETER FLUORESCENCE LIFETIME IMAGING 7
1.1 INTRODUCTION 7
1.2 FREQUENCY-DOMAIN AND TIME-DOMAIN ACQUISITION SYSTEMS 8
1.3 CONFOCAL AND WIDE-FIELD FLUORESCENCE MICROSCOPY 9
1.4 WIDE-FIELD FLIM: STATE OF THE ART 12
1.4.1 THE CONCEPT OF PHOTON ECONOMY 12
1.4.2 WIDE-FIELD FLIM BY TIME GATING 14
1.4.3 WIDE-FIELD FLIM IN THE FREQUENCY-DOMAIN 16
1.4.4 POSITION-SENSITIVE PHOTOMULTIPLIERS APPLIED TO WIDE-FIELD FLIM .
.. 17 1.5 THE QUADRANT ANODE PHOTOMULTIPLIER 20
1.6 THE IMAGING SPECTROMETER 21
1.7 TOTAL INTERNAL REFLECTION ILLUMINATION 22
1.8 THE WIDE-FIELD MULTI-PARAMETER FLIM SETUP (WFMP-FLIM) 26
1.8.1 THE EXPERIMENTAL SETUP 26
1.8.2 THE SAMPLE ILLUMINATION 29
1.8.3 TIME RESOLUTION AND PULSE INTERLEAVED EXCITATION 31
1.8.4 SENSITIVITY OF THE FLIM SETUP 32
1.9 CALIBRATIONS AND CORRECTIONS OF THE EXPERIMENTAL SETUP 35
1.9.1 CORRECTION OF THE GEOMETRICAL DISTORTION 37
1.9.2 CORRECTION OF THE DETECTOR COLOR EFFECT 38
1.10 DISCUSSION 42
2 DATA ANALYSIS OF TIME-DOMAIN FLIM EXPERIMENTS 49
2.1 INTRODUCTION 49
2.2 STATISTICAL LIMITS TO THE DATA ANALYSIS 50
2.3 OVERVIEW ON THE DATA ANALYSIS TECHNIQUES 51
BIBLIOGRAFISCHE INFORMATIONEN HTTP://D-NB.INFO/1014250811
DIGITALISIERT DURCH
IMAGE 2
2 CONTENTS
2.4 THE MODEL FUNCTION 53
2.5 THE MAXIMUM LIKELIHOOD APPROACH 56
2.6 METHODS TO MAXIMIZE THE LIKELIHOOD FUNCTION 58
2.7 IMPLEMENTATION OF THE FIT PROGRAM FOR FLIM DATA SETS 61
2.8 MONTE CARLO TEST OF THE ESTIMATION ACCURACY 62
2.9 TEST OF THE PERFORMANCE OF THE ANALYSIS ON REAL DATA 66
2.10 DISCUSSION 68
2.11 CONCLUSIONS 69
3 LONG-TERM FLIM IMAGING ON LIVING CELLS 75
3.1 INTRODUCTION 75
3.2 THE FOERSTER RESONANCE ENERGY TRANSFER 76
3.2.1 CLASSICAL ELECTRODYNAMICS APPROACH 77
3.2.2 QUANTUM MECHANICAL APPROACH 79
3.3 PRACTICAL CONSIDERATIONS ABOUT FRET 83
3.4 OVERVIEW ABOUT THE FRET MEASUREMENT IN LIVING CELLS 85
3.5 THE EGFP-TAGRFP FRET PAIR 88
3.6 DESIGN AND EXPRESSION OF THE FRET CONSTRUCT 92
3.7 STEADY-STATE FLUORESCENCE EMISSION SPECTRA OF SINGLE CELLS 93 3.8
MEASUREMENT OF PHOTOBLEACHING AND PRODUCTION OF ROS 95
3.9 LIFETIME FRET MEASUREMENTS IN HELA CELLS 98
3.10 MITOCHONDRIAL TRAFFICKING IN NEURONAL CELL CULTURES 103
3.11 THE AXONAL TRANSPORT IN NEURONS 105
3.12 KYMOGRAMS AND LIFETIME-KYMOGRAMS 106
3.13 DISCUSSION 106
SUMMARY 115
APPENDIX 117
A CONVERGENCE UNDER THE GLOBAL ANALYSIS APPROXIMATION 117
B EINSTEIN COEFFICIENTS AND OBSERVABLES 119
LIST OF ABBREVIATIONS 123
LIST OF PUBLICATIONS 125
ACKNOWLEDGEMENTS 127
|
| any_adam_object | 1 |
| author | Vitali, Marco |
| author_facet | Vitali, Marco |
| author_role | aut |
| author_sort | Vitali, Marco |
| author_variant | m v mv |
| building | Verbundindex |
| bvnumber | BV039519705 |
| ctrlnum | (OCoLC)794533247 (DE-599)BVBBV039519705 |
| dewey-full | 571.60282 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 571 - Physiology & related subjects |
| dewey-raw | 571.60282 |
| dewey-search | 571.60282 |
| dewey-sort | 3571.60282 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01751nam a2200409 c 4500</leader><controlfield tag="001">BV039519705</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">00000000000000.0</controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">110809s2011 ad|| m||| 00||| eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9783866649897</subfield><subfield code="9">978-3-86664-989-7</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)794533247</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV039519705</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-83</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">571.60282</subfield><subfield code="2">22/ger</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Vitali, Marco</subfield><subfield code="e">Verfasser</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Long term observation of living cells by wide field fluorescence lifetime imaging microscopy</subfield><subfield code="c">vorgelegt von Marco Vitali</subfield></datafield><datafield tag="246" ind1="1" ind2=" "><subfield code="a">Long-term observation of living cells by wide-field fluorescence lifetime imaging microscopy</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Berlin</subfield><subfield code="b">mbv, Mensch-und-Buch-Verl.</subfield><subfield code="c">2011</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">128 S.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">Chemical science</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="a">Zugl.: Berlin, Techn. Univ., Diss., 2011</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Fluoreszenz-Resonanz-Energie-Transfer</subfield><subfield code="0">(DE-588)4773018-3</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Fluoreszenzmikroskop</subfield><subfield code="0">(DE-588)4328299-4</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Zelle</subfield><subfield code="0">(DE-588)4067537-3</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Zelle</subfield><subfield code="0">(DE-588)4067537-3</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Fluoreszenzmikroskop</subfield><subfield code="0">(DE-588)4328299-4</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="2"><subfield code="a">Fluoreszenz-Resonanz-Energie-Transfer</subfield><subfield code="0">(DE-588)4773018-3</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024372225&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-024372225</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV039519705 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T00:05:22Z |
| institution | BVB |
| isbn | 9783866649897 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-024372225 |
| oclc_num | 794533247 |
| open_access_boolean | |
| owner | DE-83 |
| owner_facet | DE-83 |
| physical | 128 S. Ill., graph. Darst. |
| publishDate | 2011 |
| publishDateSearch | 2011 |
| publishDateSort | 2011 |
| publisher | mbv, Mensch-und-Buch-Verl. |
| record_format | marc |
| series2 | Chemical science |
| spelling | Vitali, Marco Verfasser aut Long term observation of living cells by wide field fluorescence lifetime imaging microscopy vorgelegt von Marco Vitali Long-term observation of living cells by wide-field fluorescence lifetime imaging microscopy Berlin mbv, Mensch-und-Buch-Verl. 2011 128 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Chemical science Zugl.: Berlin, Techn. Univ., Diss., 2011 Fluoreszenz-Resonanz-Energie-Transfer (DE-588)4773018-3 gnd rswk-swf Fluoreszenzmikroskop (DE-588)4328299-4 gnd rswk-swf Zelle (DE-588)4067537-3 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Zelle (DE-588)4067537-3 s Fluoreszenzmikroskop (DE-588)4328299-4 s Fluoreszenz-Resonanz-Energie-Transfer (DE-588)4773018-3 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024372225&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Vitali, Marco Long term observation of living cells by wide field fluorescence lifetime imaging microscopy Fluoreszenz-Resonanz-Energie-Transfer (DE-588)4773018-3 gnd Fluoreszenzmikroskop (DE-588)4328299-4 gnd Zelle (DE-588)4067537-3 gnd |
| subject_GND | (DE-588)4773018-3 (DE-588)4328299-4 (DE-588)4067537-3 (DE-588)4113937-9 |
| title | Long term observation of living cells by wide field fluorescence lifetime imaging microscopy |
| title_alt | Long-term observation of living cells by wide-field fluorescence lifetime imaging microscopy |
| title_auth | Long term observation of living cells by wide field fluorescence lifetime imaging microscopy |
| title_exact_search | Long term observation of living cells by wide field fluorescence lifetime imaging microscopy |
| title_full | Long term observation of living cells by wide field fluorescence lifetime imaging microscopy vorgelegt von Marco Vitali |
| title_fullStr | Long term observation of living cells by wide field fluorescence lifetime imaging microscopy vorgelegt von Marco Vitali |
| title_full_unstemmed | Long term observation of living cells by wide field fluorescence lifetime imaging microscopy vorgelegt von Marco Vitali |
| title_short | Long term observation of living cells by wide field fluorescence lifetime imaging microscopy |
| title_sort | long term observation of living cells by wide field fluorescence lifetime imaging microscopy |
| topic | Fluoreszenz-Resonanz-Energie-Transfer (DE-588)4773018-3 gnd Fluoreszenzmikroskop (DE-588)4328299-4 gnd Zelle (DE-588)4067537-3 gnd |
| topic_facet | Fluoreszenz-Resonanz-Energie-Transfer Fluoreszenzmikroskop Zelle Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024372225&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT vitalimarco longtermobservationoflivingcellsbywidefieldfluorescencelifetimeimagingmicroscopy |