Integrin and cell adhesion molecules: methods and protocols
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York [u.a.]
Humana Press
2011
|
| Schriftenreihe: | Methods in molecular biology
757 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XVI, 529 S. Ill., graph. Darst. |
| ISBN: | 1617791652 9781617791659 |
Internformat
MARC
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Datensatz im Suchindex
| _version_ | 1804148017509433344 |
|---|---|
| adam_text | Titel: Integrin and cell adhesion molecules
Autor: Shimaoka, Motomu
Jahr: 2011
Contents
Preface............................................................ v
Contributors........................................................ xiii
Part I Basic Protocols for the Study of Integrin and Related Cell
Adhesion Molecule Functionality In Vitro
1 Overview: Assays for Studying Integrin-Dependent Cell Adhesion........... 3
Alexandre Chigaev and Larry A. Sklar
2 Cell Adhesion Assays............................................. 15
Gabriele Weite-Schmidt and Stéphanie Chreng
3 Real-Time Analysis of Integrin-Dependent Transendothelial Migration
and Integrin-Independent Interstitial Motility of Leukocytes................ 31
Ziv Shulman and Ronen Alon
4 Lentiviral Gene Transfer Method to Study Integrin Function
in T Lymphocytes................................................ 47
Daliya Banerjee and Motomu Shimaoka
5 Surface Plasmon Resonance Biosensing in Studies of the Binding Between ß2
Integrin I Domains and Their Ligands................................ 55
Thomas Vorup-Jensen
6 Cell-Free Ligand-Binding Assays for Integrin LFA-1...................... 73
Koichi Yuki
Part II Structural Biology Approaches for Studying Integrins
and Related Cell Adhesion Molecules
7 Overview: Structural Biology of Integrins.............................. 81
Guanyuan Fu, Wei Wang, and Bing-Hao Luo
8 Protein Expression and Purification of Integrin I Domains
and IgSF Ligands for Crystallography................................. 101
Hongmin Zhang andjia-huai Wang
9 Electron Microscopic Imaging of Integrin.............................. Ill
Kenji Iwasaki
10 An NMR Method to Study Protein-Protein Interactions.................. 129
Noritaka Nishida and Ichio Shimada
11 Single-Molecule Methods to Study Cell Adhesion Molecules............... 139
Joonil Seog
Part III Imaging for Investigating Cell Adhesion and Migration
12 Overview: Imaging in the Study of Integrins............................ 159
Christopher V. Carman
IX
x Contents
13 Live Imaging of LFA-1-Dependent T-Cell Motility and Stop Signals.......... 191
Andrew J. Wiemer, Sarah Wernimont, and Anna Huttenlocher
14 Monitoring Integrin Activation by Fluorescence Resonance
Energy Transfer................................................. 205
Craig T. Lefort, Young-Min Hyun, and Minsoo Kim
15 High-Resolution Fluorescence Microscopy to Study
Transendothelial Migration......................................... 215
Christopher V. Carman
16 Multiphoton Intravital Microscopy to Study Lymphocyte Motility
in Lymph Nodes................................................. 247
Thomas T. Murooka and Thorsten R. Mempel
Part IV Signaling Through Cell Adhesion Molecules
17 Overview of Integrin Signaling in the Immune System.................... 261
Tatsuo Kinashi
18 Rapl and Integrin Inside-Out Signaling............................... 279
Koko Katagiri and Tatsuo Kinashi
19 Isolation of Focal Adhesion Proteins for Biochemical
and Proteomic Analysis............................................ 297
Jean-Cheng Kuo, Xuemei Han, John R. Yates III,
and Clare M. Waterman
20 Talin and Signaling Through Integrins................................ 325
Mohamed Bouaouina, David S. Harburger,
and David A. Calderwood
21 Proteomics Method for Identification of Pseudopodium
Phosphotyrosine Proteins.......................................... 349
Yingchun Wang and Richard L. Klemke
Part V Cell Adhesion and Migration at Organismal Levels
in a Physiological Context
22 Overview: Studying Integrins In Vivo................................. 369
Clifford A. Lowell and Tanya N. Mayadas
23 A Method for the Generation of Conditional Gene-Targeted Mice........... 399
Masahiro Yamamoto and Kiyoshi Takeda
24 T-Cell Homing to the Gut Mucosa: General Concepts
and Methodological Considerations.................................. 411
Jaime De Calisto, EduardoJ. Villabianca, Sen Wang,
Maria R. Bono, Mario Rosemblatt, and J. Rodrigo Mora
25 In Vivo Quantitative Proteomics: The SILAC Mouse..................... 435
Sara Zanivan, Marcus Krueger, and Matthias Mann
26 Analysis of Chemotaxis in Dictyostelium............................... 451
Huaqing Cai, Chuan-Hsiang Huang, Peter N. Devreotes,
and Miho Iijima
Contents xi
Part VI Methods and Technologies Towards Novel Therapeutics
and Diagnostics Targeting Integrins
27 Integrins in Tumor Angiogenesis and Lymphangiogenesis................. 471
Philippe Foubert and Judith A. Vamer
28 PET-Radioimmunodetection of Integrins: Imaging Acute Colitis
Using a 64Cu-Labeled Anti-ß7 Integrin Antibody......................... 487
Jason L.J. Dearling and Alan B. Packard
29 Integrin-Targeted Nanoparticles for siRNA Delivery...................... 497
Noa Ben-Arie, Ranit Kedmi, and Dan Peer
30 Humanized Mice for Studying Human Leukocyte Integrins In Vivo.......... 509
Sang-Soo Kim, Priti Kumar, Chunting Ye, and Premlata Shankar
Index................................................................. 523
|
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| id | DE-604.BV039166203 |
| illustrated | Illustrated |
| indexdate | 2024-07-10T00:00:27Z |
| institution | BVB |
| isbn | 1617791652 9781617791659 |
| language | English |
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| physical | XVI, 529 S. Ill., graph. Darst. |
| publishDate | 2011 |
| publishDateSearch | 2011 |
| publishDateSort | 2011 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | Integrin and cell adhesion molecules methods and protocols ed. by Motomu Shimaoka New York [u.a.] Humana Press 2011 XVI, 529 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 757 Springer protocols Integrine (DE-588)4278201-6 gnd rswk-swf Labortechnik (DE-588)4123602-6 gnd rswk-swf Zell-Adhäsionsmolekül (DE-588)4300852-5 gnd rswk-swf Zell-Adhäsionsmolekül (DE-588)4300852-5 s Labortechnik (DE-588)4123602-6 s DE-604 Integrine (DE-588)4278201-6 s b DE-604 Shimaoka, Motomu Sonstige oth Erscheint auch als Online-Ausgabe 978-1-61779-166-6 Methods in molecular biology 757 (DE-604)BV035362695 757 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024183558&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Integrin and cell adhesion molecules methods and protocols Methods in molecular biology Integrine (DE-588)4278201-6 gnd Labortechnik (DE-588)4123602-6 gnd Zell-Adhäsionsmolekül (DE-588)4300852-5 gnd |
| subject_GND | (DE-588)4278201-6 (DE-588)4123602-6 (DE-588)4300852-5 |
| title | Integrin and cell adhesion molecules methods and protocols |
| title_auth | Integrin and cell adhesion molecules methods and protocols |
| title_exact_search | Integrin and cell adhesion molecules methods and protocols |
| title_full | Integrin and cell adhesion molecules methods and protocols ed. by Motomu Shimaoka |
| title_fullStr | Integrin and cell adhesion molecules methods and protocols ed. by Motomu Shimaoka |
| title_full_unstemmed | Integrin and cell adhesion molecules methods and protocols ed. by Motomu Shimaoka |
| title_short | Integrin and cell adhesion molecules |
| title_sort | integrin and cell adhesion molecules methods and protocols |
| title_sub | methods and protocols |
| topic | Integrine (DE-588)4278201-6 gnd Labortechnik (DE-588)4123602-6 gnd Zell-Adhäsionsmolekül (DE-588)4300852-5 gnd |
| topic_facet | Integrine Labortechnik Zell-Adhäsionsmolekül |
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