Mammalian cell viability: methods and protocols
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York
Humana Press
2011
|
| Schriftenreihe: | Methods in molecular biology
740 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Includes bibliographical references and index |
| Beschreibung: | XI, 240 S. Ill., graph. Darst. |
| ISBN: | 9781617791079 |
Internformat
MARC
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| 245 | 1 | 0 | |a Mammalian cell viability |b methods and protocols |c ed. by Martin J. Stoddart |
| 264 | 1 | |a New York |b Humana Press |c 2011 | |
| 300 | |a XI, 240 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 740 | |
| 490 | 0 | |a Springer protocols | |
| 500 | |a Includes bibliographical references and index | ||
| 650 | 4 | |a Cell physiology | |
| 650 | 4 | |a Mammals / Cytology | |
| 650 | 4 | |a Cytology / Methodology | |
| 650 | 4 | |a Cells / Aging | |
| 650 | 4 | |a Cell death | |
| 650 | 4 | |a Mammals / Physiology | |
| 650 | 4 | |a Cell Survival | |
| 650 | 4 | |a Mammals / physiology | |
| 700 | 1 | |a Stoddart, Martin J. |e Sonstige |4 oth | |
| 830 | 0 | |a Methods in molecular biology |v 740 |w (DE-604)BV035362695 |9 740 | |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-024147577 | ||
Datensatz im Suchindex
| _version_ | 1804147963578023936 |
|---|---|
| adam_text | Titel: Mammalian cell viability
Autor: Stoddart, Martin J.
Jahr: 2011
Contents
Preface............................................................. p
Contributors......................................................... ix
1 Cell Viability Assays: Introduction.................................... 1
Martin J. Stoddart
2 Cell Viability Analysis Using Trypan Blue: Manual and Automated Methods..... 7
Kristine S. Louis and Andre C. Siegel
3 Estimation of Cell Number Based on Metabolic Activity:
The MTT Reduction Assay.......................................... 13
László Kupcsik
4 WST-8 Analysis of Cell Viability During Osteogenesis of Human
Mesenchymal Stem Cells............................................ 21
Martin J. Stoddart
5 Assessment of Cell Proliferation with Resazurin-Based Fluorescent Dye........ 27
EwaM. Czekanska
6 The xCELLigence System for Real-Time and Label-Free
Monitoring of Cell Viability......................................... 33
Ning Ke, Xiaobo Wang, Xiao Xu, and Yanta A. Abassi
7 Analysis of Tumor and Endothelial Cell Viability and Survival
Using Sulforhodamine B and Clonogenic Assays.......................... 45
Caroline Woolston and Stewart Martin
8 Annexin V/7-AAD Staining in Keratinocytes............................ 57
Maya Zimmermann and Norbert Meyer
9 Measurement of Caspase Activity: From Cell Populations
to Individual Cells................................................ 65
Gabriela Paroni and Claudio Brancolini
10 Rapid Quantification of Cell Viability and Apoptosis in B-Cell
Lymphoma Cultures Using Cyanine SYTO Probes........................ 81
Donald Wlodkowic, Joanna Skommer, and Zbigniew Darzynkiewicz
11 Multiplexing Cell Viability Assays..................................... 91
Helga HJ. Gerets, Stephane Dhalluin, and Franck A. Atienzar
12 Cytotoxicity Testing: Measuring Viable Cells, Dead Cells,
and Detecting Mechanism of Cell Death................................ 103
Terry L. Riss, Richard A. Moravec, and Andrew L. Niles
13 Fluorescein Diacetate for Determination of Cell Viability
in 3D Fibroblast-Collagen-GAG Constructs............................ 115
Heather M. Powell, Alexis D. Armour, and Steven T. Boyce
viii Contents
14 Confocal Imaging Protocols for Live/Dead Staining
in Three-Dimensional Carriers....................................... 127
Benjamin Gantenbein-Ritter, Christoph M. Sprecher,
Samantha Chan, Svenja Illien-Jiinger, and Sibylle Grad
15 Viability Assessment of Osteocytes Using Histological Lactate
Dehydrogenase Activity Staining on Human Cancellous Bone Sections......... 141
Katharina Jahn and Martin J. Stoddart
16 Measuring Glutamate Receptor Activation-Induced Apoptotic
Cell Death in Ischemic Rat Retina Using the TUNEL Assay................. 149
Won-Kyuju and Keun-Toung Kim
17 Exploiting the Liberation of Zn2+ to Measure Cell Viability................. 157
Christian J. Stork and Tang V. Li
18 Noninvasive Bioluminescent Quantification of Viable
Stem Cells in Engineered Constructs.................................. 165
Karim Oudina, Adeline Cambon-Binder,
and Delphine Logeart-Avramoglou
19 Raman Micro-Spectroscopy as a Non-invasive Cell Viability Test............. 179
Sophie Verrier, Alina Zoladek, and loan Notingher
20 Closed Ampoule Isothermal Microcalorimetry for Continuous
Real-Time Detection and Evaluation of Cultured Mammalian
Cell Activity and Responses.......................................... 191
Olivier Braissant and Alma U. Dan Daniels
21 Digital Image Processing of Live/Dead Staining......................... 209
Pieter Spaepen, Sebastian De Boodt, Jean-Marie Aerts,
and Jos Vander Sloten
Index.............................................................. 231
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| id | DE-604.BV039129191 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T23:59:35Z |
| institution | BVB |
| isbn | 9781617791079 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-024147577 |
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| owner | DE-12 |
| owner_facet | DE-12 |
| physical | XI, 240 S. Ill., graph. Darst. |
| publishDate | 2011 |
| publishDateSearch | 2011 |
| publishDateSort | 2011 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | Mammalian cell viability methods and protocols ed. by Martin J. Stoddart New York Humana Press 2011 XI, 240 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 740 Springer protocols Includes bibliographical references and index Cell physiology Mammals / Cytology Cytology / Methodology Cells / Aging Cell death Mammals / Physiology Cell Survival Mammals / physiology Stoddart, Martin J. Sonstige oth Methods in molecular biology 740 (DE-604)BV035362695 740 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024147577&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Mammalian cell viability methods and protocols Methods in molecular biology Cell physiology Mammals / Cytology Cytology / Methodology Cells / Aging Cell death Mammals / Physiology Cell Survival Mammals / physiology |
| title | Mammalian cell viability methods and protocols |
| title_auth | Mammalian cell viability methods and protocols |
| title_exact_search | Mammalian cell viability methods and protocols |
| title_full | Mammalian cell viability methods and protocols ed. by Martin J. Stoddart |
| title_fullStr | Mammalian cell viability methods and protocols ed. by Martin J. Stoddart |
| title_full_unstemmed | Mammalian cell viability methods and protocols ed. by Martin J. Stoddart |
| title_short | Mammalian cell viability |
| title_sort | mammalian cell viability methods and protocols |
| title_sub | methods and protocols |
| topic | Cell physiology Mammals / Cytology Cytology / Methodology Cells / Aging Cell death Mammals / Physiology Cell Survival Mammals / physiology |
| topic_facet | Cell physiology Mammals / Cytology Cytology / Methodology Cells / Aging Cell death Mammals / Physiology Cell Survival Mammals / physiology |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=024147577&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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