Ionic conductances of the ruminal epithelium:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Berlin
Mensch und Buch Verl.
2010 [erschienen] 2011
|
| Schlagworte: | |
| Online-Zugang: | Volltext Inhaltsverzeichnis |
| Beschreibung: | Nebentitel: Ionenleitfähigkeiten des Pansenepithels |
| Beschreibung: | [6], 141 S. Ill., graph. Darst. |
Internformat
MARC
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| 008 | 110531s2011 gw ad|| m||| 00||| eng d | ||
| 020 | |z 9783866649613 |9 978-3-86664-961-3 | ||
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| 100 | 1 | |a Stumpff, Friederike |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Ionic conductances of the ruminal epithelium |c eingereicht von Friederike Stumpff |
| 246 | 1 | 3 | |a Ionenleitfähigkeiten des Pansenepithels |
| 264 | 1 | |a Berlin |b Mensch und Buch Verl. |c 2010 [erschienen] 2011 | |
| 300 | |a [6], 141 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Nebentitel: Ionenleitfähigkeiten des Pansenepithels | ||
| 502 | |a Berlin, Freie Univ., Habil.-Schr., 2011 | ||
| 650 | 7 | |a rumen |2 cabt | |
| 650 | 7 | |a omasum |2 cabt | |
| 650 | 7 | |a ion channels |2 cabt | |
| 650 | 7 | |a ion transport |2 cabt | |
| 650 | 7 | |a ionophores |2 cabt | |
| 650 | 7 | |a sodium |2 cabt | |
| 650 | 7 | |a potassium |2 cabt | |
| 650 | 7 | |a chloride |2 cabt | |
| 650 | 7 | |a magnesium |2 cabt | |
| 650 | 7 | |a ammonium |2 cabt | |
| 650 | 7 | |a ammonia |2 cabt | |
| 650 | 7 | |a urea |2 cabt | |
| 650 | 7 | |a acetate |2 cabt | |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 776 | 0 | 8 | |i Erscheint auch als |n Online-Ausgabe |o urn:nbn:de:kobv:188-fudissthesis000000022641-5 |
| 856 | 4 | 1 | |u http://www.diss.fu-berlin.de/diss/receive/FUDISS_thesis_000000022641 |z kostenfrei |3 Volltext |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022582234&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 912 | |a ebook | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-022582234 | ||
Datensatz im Suchindex
| _version_ | 1804145736377434112 |
|---|---|
| adam_text | IMAGE 1
CONTENTS
1 INTRODUCTION 1
2 OPEN QUESTIONS 2
3 THE FORESTOMACH SYSTEM: AN OVERVIEW 5
3.1.1 HISTOLOGY AND ORGANOGENESIS OF THE FORESTOMACHS 5
3.1.2 SALIVA 7
3.1.3 RELEASE OF CATIONS AND ANIONS INTO THE RUMEN BY FERMENTATIONAL
PROCESSES 8
3.1.4 CLINICALIMPORTANCE OF MAINTAINING RUMINAL HOMEOSTASIS 11
3.1.5 CLINICAL IMPORTANCE OF OMASAL ABSORPTION 11
3.1.6 IMPORTANCE OF DEVELOPING TRANSPORT MODELS ACROSS FORESTOMACH
EPITHELIA 12
4 LITERATURE 13
4.1 PARACELLULAR PATHWAY 13
4.1.1 TRANSPORT OF CHLORIDE ACROSS FORESTOMACH EPITHELIA: A
TRANSCELLULAR PROCESS 14
4.1.2 PARACELLULAR TRANSPORT: THE RUMEN 15
4.1.3 PARACELLULAR TRANSPORT: THE OMASUM 16
4.1.4 PARACELLULAR TRANSPORT: THE RETICULUM 17
4.1.5 OPEN QUESTIONS 18
4.2 THE STRATUM CORNEUM AND THE APICAL MICROCLIMATE 18
4.2.1 THE FUNCTIONAL SIGNIFICANCE OF THE APICAL MICROCLIMATE 18
4.2.2 OPEN QUESTIONS 19
4.3 BASOLATERAL EXTRUSION OF NA* VIA THE NA*/K*-ATPASE 20
4.4 APICAL UPTAKE OF SODIUM VIA NA*/H*- EXCHANGE (NHE) 20
4.4.1 STIMULATION OF NHE BY CO 2 AND SHORT CHAIN FATTY ACIDS (SCFA) 21
4.4.2 OPEN QUESTIONS 22
4.5 ELECTROGENIC NA* CONDUCTANCE OF THE RUMEN 23
4.5.1 INTERACTIONS OF NA* CONDUCTANCE WITH CA 2*ANDMG2* 24
4.5.2 EFFECTS OF VOLTAGE ON ELECTROGENIC NA* TRANSPORT 25
4.5.3 OPEN QUESTIONS 26
BIBLIOGRAFISCHE INFORMATIONEN HTTP://D-NB.INFO/1011457555
DIGITALISIERT DURCH
IMAGE 2
IONIC CONDUCTANCES OF THE RUMLNAL EPITHELIUM
4.6 TRANSPORT OF MAGNESIUM 26
4.6.1 APICAL UPTAKE OF MAGNESIUM 27
4.6.2 BASOLATERAL EFFLUX OF MG 2* 28
4.6.3 OPEN QUESTIONS 28
4.7 TRANSPORT OF POTASSIUM 28
4.7.1 POTASSIUM CONDUCTANCES OF THE RUMEN 29
4.7.2 THE INVERSE REGULATION OF RUMINAL NA* AND K* CONCENTRATION 29
4.7.3 OPEN QUESTIONS 29
4.8 TRANSPORT OF NITROGEN ACROSS THE RUMINAL WALL .-. 30
4.9 ABSORPTION OF AMMONIA FROM THE RUMEN 31
4.9.1 THE RUMINAL TRANSPORT OF AMMONIA: NH 3 OR NH4*? 32
4.9.2 OPEN QUESTIONS 33
4.10 SECRETION OF UREA INTO THE RUMEN 33
4.10.1 OPEN QUESTIONS 34
4.11 TRANSPORT OF CHLORIDE AND BICARBONATE 34
4.11.1 DIFFUSIONAL UPTAKE OF CO 2 34
4.11.2 APICAL CI7HCO 3 EXCHANGER 35
4.11.3 OPEN QUESTIONS 36
4.12 ABSORPTION OF SCFA FROM THE RUMEN 36
4.12.1 PRODUCTION OF SCFA 36
4.12.2 UPTAKE OF SCFA OCCURS TRANSCELLULARLY 37
4.12.3 APICAL UPTAKE OF SCFA 37
4.12.4 METABOLISM OF SCFA 39
4.12.5 BASOLATERAL EFFLUX OF SCFA 39
4.12.6 OPEN QUESTIONS 40
4.13 TRANSPORT OF ANIONS ACROSS THE OMASUM 40
4.13.1 OPEN QUESTIONS 41
5 METHODS 41
5.1 CALCULATION OF THE UPPER LIMIT FOR PARACELLULAR TRANSPORT FROM THE
PARTIAL CONDUCTANCE EQUATION 41
5.2 ISOLATION OF CELLS FROM THE RUMEN 42
5.3 ESTABLISHMENT OF A METHOD FOR THE ISOLATION OF CELLS FROM THE OMASUM
43
IMAGE 3
CONTENTS
5.4 CONFOCAL LASER SCANNING MICROSCOPY AND IMMUNOHISTOCHEMISTRY 44
5.4.1 HE STAINING AND STAINING FOR CYTOKERATIN AND VIMENTIN 45
5.4.2 STAINING FOR TIGHT JUNCTION PROTEINS 46
5.5 PATCH-CLAMP MEASUREMENTS 47
5.5.1 ELECTROPHYSIOLOGY 48
5.5.2 PERFUSION OF THE BATH CHAMBER 52
5.5.3 SOLUTIONS AND CHEMICALS 52
5.5.4 WHOLE CELL CURRENTS 53
5.5.5 REVERSAL POTENTIALS AND RELATIVE PERMEABILITY RATIO 54
5.5.6 SINGLE-CHANNEL CONDUCTANCES 55
5.6 MICROELECTRODE EXPERIMENTS 56
5.6.1 EXPERIMENTAL SET-UP 56
5.6.2 FABRICATION OF DOUBLE-BARRELED PH-SENSITIVE MICROELECTRODES 57
5.6.3 MEASUREMENTS 59
5.7 STATISTICS 59
6 RESULTS 59
6.1 IDENTIFYING THE PROTEINS OF THE PARACELLULAR PATHWAY 59
6.2 APICAL MICROCLIMATE 63
6.3 ELECTROGENIC UPTAKE OF NA*, CA 2*, AND MG2* BY THE RUMEN: THE
NON-SELECTIVE CATION
CHANNEL 65
6.3.1 ELECTROGENIC CONDUCTANCE FOR SODIUM: INTERACTION WITH MG 2* 66
6.3.2 PERMEABILITY TO CA 2* AND MG2* 68
6.3.3 MAGNESIUM AND THE VOLTAGE DEPENDENCE OF THE RUMINAL SODIUM
CONDUCTANCE 69
6.3.4 INTERACTIONS OF THE NON-SELECTIVE CATION CHANNEL WITH SCFA 71
6.3.5 INTERACTIONS OF THE NON-SELECTIVE CATION CHANNEL WITH CAMP 71
6.3.6 THE BASOLATERAL EFFLUX OF MG 2* 72
6.3.7 INTERACTION OF THE NON-SELECTIVE CATION CHANNEL(S) WITH BLOCKERS
72
6.4 CONDUCTANCE FOR POTASSIUM 73
6.4.1 INWARDLY RECTIFYING POTASSIUM CONDUCTANCE 73
6.4.2 BLOCKER SENSITIVITY 74
6.4.3 OUTWARDLY RECTIFYING POTASSIUM CONDUCTANCE 74
6.4.4 A MODEL FOR RUMINAL POTASSIUM TRANSPORT 75
IMAGE 4
IONIC CONDUCTANCES OF THE RUMINAL EPITHELIUM
6.5 THE CONDUCTANCE OF THE RUMEN FOR AMMONIUM 76
6.5.1 PERMEABILITY OF THE INTACT RUMEN TO NH 3 AND NH4* 77
6.5.2 ISOLATED CELLS OF THE RUMINAL EPITHELIUM EXPRESS CHANNELS
PERMEABLE TO NH 4* 77
6.5.3 INTERACTION OF NH3/NH4* TRANSPORT WITH RUMINAL SODIUM TRANSPORT 78
6.5.4 THE BASOLATERAL EFFLUX OF NH 4* 79
6.6 THE SECRETION OF UREA BY THE RUMEN 81
6.6.1 TRANSPORT OF UREA IS MEDIATED BY A TRANSPORT PROTEIN 81
6.6.2 UREA RECYCLING AND PH HOMEOSTASIS 82
6.7 APICAL UPTAKE OF SCFA 82
6.8 DISTRIBUTION THEORY ACROSS EPITHELIA OR WHY LIPID DIFFUSION CANNOT
WORK 84
6.9 BASOLATERAL EFFLUX OF CHLORIDE AND SCFA: THE MAXI-ANION CHANNEL 87
6.9.1 THE LARGE-CONDUCTANCE ANION CHANNEL OF THE RUMEN 87
6.9.2 SENSITIVITY TO BLOCKERS 89
6.9.3 POTENTIAL DEPENDENCE AND THE THREE STATE GATING MODEL 89
6.9.4 HALIDE SELECTIVITY SEQUENCE 91
6.9.5 PERMEABILITY OF THE RUMINAL ANION CHANNEL TO THE ANIONS OF SCFA 93
6.9.6 INTERACTIONS OF SCFA WITH THE NON-SELECTIVE CATION CHANNEL OF THE
RUMEN 94
6.9.7 THE LARGE-CONDUCTANCE ANION CHANNEL OF THE OMASUM 95
6.9.8 A MODEL FOR THE EFFLUX OF SCFA FROM THE FORESTOMACHS 96
7 DISCUSSION 99
7.1 THE APICAL NON-SELECTIVE CATION CONDUCTANCE OF THE RUMINAL
EPITHELIUM 99 7.1.1 SODIUM, MAGNESIUM, AND THE POTASSIUM HOMEOSTASIS OF
THE RUMINANT 99
7.2 UREA, AMMONIA AND PH HOMEOSTASIS 102
7.2.1 THE NON-SELECTIVE CATION CHANNEL AS A PATHWAY FOR THE APICAL
UPTAKE OF NH 4* 103
7.2.2 THE BASOLATERAL K* CHANNEL AS A PATHWAY FOR THE EFFLUX OF NH 4*
103
7.2.3 THE INTERACTIONS BETWEEN AMMONIA AND RUMINAL NA* AND H* TRANSPORT
104
7.2.4 UREA, AMMONIA, AND PH HOMEOSTASIS 105
7.3 SCFA AND THE BASOLATERAL ANION CHANNEL 107
7.3.1 SALIVA IS NEEDED FOR RUMINAL BUFFERING 107
7.3.2 THE COUPLED TRANSPORT OF SCFA AND NA* 108
7.3.3 BASOLATERAL EFFLUX OF SCFA FROM FORESTOMACH EPITHELIA THROUGH A
MAXI ANION CHANNEL 109
7.3.4 MULTIPLE PATHWAYS MEDIATE THE EFFLUX OF SCFA FROM THE RUMEN 111
8 SUMMARY 113
IMAGE 5
CONTENTS
9 REFERENCES 115
10 ABBREVIATIONS 136
11 ACKNOWLEDGMENTS 138
12 APPENDIX 139
|
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| dewey-ones | 630 - Agriculture and related technologies |
| dewey-raw | 630 |
| dewey-search | 630 |
| dewey-sort | 3630 |
| dewey-tens | 630 - Agriculture and related technologies |
| discipline | Agrar-/Forst-/Ernährungs-/Haushaltswissenschaft / Gartenbau |
| format | Thesis Book |
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| indexdate | 2024-07-09T23:24:11Z |
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| language | English |
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| spelling | Stumpff, Friederike Verfasser aut Ionic conductances of the ruminal epithelium eingereicht von Friederike Stumpff Ionenleitfähigkeiten des Pansenepithels Berlin Mensch und Buch Verl. 2010 [erschienen] 2011 [6], 141 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Nebentitel: Ionenleitfähigkeiten des Pansenepithels Berlin, Freie Univ., Habil.-Schr., 2011 rumen cabt omasum cabt ion channels cabt ion transport cabt ionophores cabt sodium cabt potassium cabt chloride cabt magnesium cabt ammonium cabt ammonia cabt urea cabt acetate cabt (DE-588)4113937-9 Hochschulschrift gnd-content Erscheint auch als Online-Ausgabe urn:nbn:de:kobv:188-fudissthesis000000022641-5 http://www.diss.fu-berlin.de/diss/receive/FUDISS_thesis_000000022641 kostenfrei Volltext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022582234&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Stumpff, Friederike Ionic conductances of the ruminal epithelium rumen cabt omasum cabt ion channels cabt ion transport cabt ionophores cabt sodium cabt potassium cabt chloride cabt magnesium cabt ammonium cabt ammonia cabt urea cabt acetate cabt |
| subject_GND | (DE-588)4113937-9 |
| title | Ionic conductances of the ruminal epithelium |
| title_alt | Ionenleitfähigkeiten des Pansenepithels |
| title_auth | Ionic conductances of the ruminal epithelium |
| title_exact_search | Ionic conductances of the ruminal epithelium |
| title_full | Ionic conductances of the ruminal epithelium eingereicht von Friederike Stumpff |
| title_fullStr | Ionic conductances of the ruminal epithelium eingereicht von Friederike Stumpff |
| title_full_unstemmed | Ionic conductances of the ruminal epithelium eingereicht von Friederike Stumpff |
| title_short | Ionic conductances of the ruminal epithelium |
| title_sort | ionic conductances of the ruminal epithelium |
| topic | rumen cabt omasum cabt ion channels cabt ion transport cabt ionophores cabt sodium cabt potassium cabt chloride cabt magnesium cabt ammonium cabt ammonia cabt urea cabt acetate cabt |
| topic_facet | rumen omasum ion channels ion transport ionophores sodium potassium chloride magnesium ammonium ammonia urea acetate Hochschulschrift |
| url | http://www.diss.fu-berlin.de/diss/receive/FUDISS_thesis_000000022641 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022582234&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT stumpfffriederike ionicconductancesoftheruminalepithelium AT stumpfffriederike ionenleitfahigkeitendespansenepithels |