cDNA libraries: methods and applications
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York [u.a.]
Humana Press
2011
|
| Schriftenreihe: | Methods in molecular biology
729 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Includes bibliographical references and index |
| Beschreibung: | XI, 275 S. Ill. 27 cm |
| ISBN: | 9781617790645 1617790648 |
Internformat
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| 490 | 1 | |a Methods in molecular biology |v 729 | |
| 500 | |a Includes bibliographical references and index | ||
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| 650 | 4 | |a DNA, Complementary | |
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Datensatz im Suchindex
| _version_ | 1804145682437636096 |
|---|---|
| adam_text | Titel: cDNA libraries
Autor: Lu, Chaofu
Jahr: 2011
Contents
Preface............................................................. p
Contributors......................................................... ix
Part I Enhanced Approaches to Inherent Problems
1 Isolation of Fungal Infection Structures from Plant Tissue by Flow
Cytometry for Cell-Specific Transcriptome Analysis........................ 3
Hiroyuki Takahara, Elmar Endl, and Richard O Connell
2 Preparation of a High-Quality cDNA Library from a Single-Cell Quantity
of mRNA Using Chum-RNA........................................ 15
Hiroshi Nojima and Takahiro Tougan
3 Construction of a Full-Length cDNA Library from Castor Endosperm
for High-Throughput Functional Screening............................. 37
Chaqfu Lu, James G. Wallis, and John Browse
4 Full-Length Transcriptome Analysis Using a Bias-Free cDNA Library
Prepared with the Vector-Capping Method.............................. 53
Seishi Kato, Mio Oshikawa, and Kuniyo Ohtoko
5 Construction of Improved Yeast Two-Hybrid Libraries..................... 71
Richard H. Maier, Christina J. Maier, andKamil under
6 Normalization of Full-Length-Enriched cDNA.......................... 85
Ekaterina A, Boßdanova, Ekaterina V. Barsova, Irina A. Shagina,
Alexander Scheglov, Veronika Anisimova, Laura L. Vagner,
Sergey A, Lukyanov, and Dmitry A. Shagin
7 Bioinformatic Methods for Finding Differentially Expressed Genes in cDNA
Libraries, Applied to the Identification of Tumour Vascular Targets............ 99
John M.J. Herbert, DovJ. Stekel, Manuela Mura, Michail Sychev,
and Roy Bicknell
Part II Visionary Applications
8 Enzymatic Production of RNAi Libraries from cDNAs and High-Throughput
Selection of Effective shRNA Expression Constructs....................... 123
Kohtaroh Sugao and Kenzo Hirose
9 Construction of Small RNA cDNA Libraries for High-Throughput
Sequencing...................................................... 141
Cheng Lu and Vikas Shedge
10 Focusing Mutations Within Random Libraries to Distinct Areas:
Protein Domain Library Generation by Overlap Extension.................. 153
Andreas Grate and Joachim Jose
11 Generation of Families of Construct Variants Using Golden Gate Shuffling..... 167
Carota Engler and Sylvestre MariUonnet
vin Contents
12 Application of Full-Length cDNA Resources to Gain-of-Function
Technology for Characterization of Plant Gene Function................... 183
Youichi Kondou, Mieko Higuchi, Takanari Ichikawa, and Minami Matsui
13 Construction of Yeast Surface-Displayed cDNA Libraries................... 199
Scott Bidlingmaier and Bin Liu
14 Identification of Protein/Target Molecule Interactions Using Yeast
Surface-Displayed cDNA Libraries.................................... 211
Scott Bidlingmaier and Bin Liu
15 SNP Discovery by Transcriptome Pyrosequencing........................ 225
W. Brad Barbazuk and Patrick S. Schnäble
16 RNA-Seq Analysis of Gene Expression and Alternative Splicing
by Double-Random Priming Strategy.................................. 247
Michael T. Lovci, Hai-Ri Li, Xiang-Dong Fu, and Gene W. Teo
17 Generation of a Large Catalog of Unique Transcripts for Whole-Genome
Expression Analysis in Nonmodel Species............................... 257
Diana Bellin, Alberto Ferrarini, and Massimo Deltedonne
Index.................................................................. 271
|
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| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.86 |
| dewey-search | 572.86 |
| dewey-sort | 3572.86 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Book |
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| id | DE-604.BV037392682 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T23:23:20Z |
| institution | BVB |
| isbn | 9781617790645 1617790648 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-022545533 |
| oclc_num | 701812651 |
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| owner | DE-12 DE-29T DE-11 |
| owner_facet | DE-12 DE-29T DE-11 |
| physical | XI, 275 S. Ill. 27 cm |
| publishDate | 2011 |
| publishDateSearch | 2011 |
| publishDateSort | 2011 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | cDNA libraries methods and applications ed. by Chaofu Lu ... New York [u.a.] Humana Press 2011 XI, 275 S. Ill. 27 cm txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 729 Includes bibliographical references and index Antisense DNA. DNA, Complementary Lu, Chaofu Sonstige oth Methods in molecular biology 729 (DE-604)BV035362695 729 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022545533&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | cDNA libraries methods and applications Methods in molecular biology Antisense DNA. DNA, Complementary |
| title | cDNA libraries methods and applications |
| title_auth | cDNA libraries methods and applications |
| title_exact_search | cDNA libraries methods and applications |
| title_full | cDNA libraries methods and applications ed. by Chaofu Lu ... |
| title_fullStr | cDNA libraries methods and applications ed. by Chaofu Lu ... |
| title_full_unstemmed | cDNA libraries methods and applications ed. by Chaofu Lu ... |
| title_short | cDNA libraries |
| title_sort | cdna libraries methods and applications |
| title_sub | methods and applications |
| topic | Antisense DNA. DNA, Complementary |
| topic_facet | Antisense DNA. DNA, Complementary |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022545533&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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