Signal transduction immunohistochemistry: methods and protocols
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Humana
2011
|
| Schriftenreihe: | Methods in molecular biology
717 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Includes bibliographical references and index |
| Beschreibung: | xiii, 302 p. ill. 27 cm |
| ISBN: | 9781617790232 1617790230 |
Internformat
MARC
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| 020 | |a 9781617790232 |9 978-1-61779-023-2 | ||
| 020 | |a 1617790230 |9 1-61779-023-0 | ||
| 035 | |a (OCoLC)690089416 | ||
| 035 | |a (DE-599)BVBBV037392672 | ||
| 040 | |a DE-604 |b ger |e rakwb | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-12 | ||
| 245 | 1 | 0 | |a Signal transduction immunohistochemistry |b methods and protocols |c ed. by Alexander E. Kalyuzhny |
| 264 | 1 | |a New York, NY |b Humana |c 2011 | |
| 300 | |a xiii, 302 p. |b ill. |c 27 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 717 | |
| 500 | |a Includes bibliographical references and index | ||
| 650 | 4 | |a Cellular signal transduction | |
| 650 | 4 | |a Immunohistochemistry | |
| 650 | 4 | |a Signal Transduction | |
| 700 | 1 | |a Kaljužnyj, Aleksandr |e Sonstige |4 oth | |
| 830 | 0 | |a Methods in molecular biology |v 717 |w (DE-604)BV035362695 |9 717 | |
| 856 | 4 | 2 | |m HBZ Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022545526&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
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Datensatz im Suchindex
| _version_ | 1804145682420858880 |
|---|---|
| adam_text | Titel: Signal transduction immunohistochemistry
Autor: Kalyuzhny, Alexander E.
Jahr: 2011
Contents
Preface................................................................. vit
Contributors............................................................. xi
Part I Antibodies as a Tool: From Concept
to Design and Application
1 Overview of the Generation, Validation, and Application
of Phosphosite-Specific Antibodies.................................... 3
Kathy Brumbaugh, Wade Johnson, Wen-Chieh Liao,
Mong-ShangLin, J.P. Houchins, Jeff Cooper, Steven Stoesz,
and Roberto Campos-Gonzalez
2 Selection and Validation of Antibodies for Signal Transduction
Immunohistochemistry............................................. 45
JurajBodo and Eric D. Hsi
3 An Overview of Western Blotting for Determining Antibody
Specificities for Immunohistochemistry................................. 55
Biß T. Kurien, YaserDorri, Skyler Dillon, AnilDsouza,
and R. Hal Scofield
4 Optimized Protocol to Make Phospho-Specific Antibodies that Work.......... 69
Amy J. Archuleta, Crystal A. Stutzke, Kristin M. Nixon,
and Michael D. Browning
Part II Preservation and Unmasking of Tissue Antigens
5 Methodology and Technology for Stabilization of Specific
States of Signal Transduction Proteins.................................. 91
Mats Borén
6 An Enhanced Antigen-Retrieval Protocol for Immunohistochemical
Staining of Formalin-Fixed, Paraffin-Embedded Tissues.................... 101
Sergei I. Syrbu and Michael B. Cohen
Part III Imaging Techniques and High-Throughput Data Analysis
7 Imaging Techniques in Signal Transduction IHC......................... 113
Jerry Sedgewick
8 Practical Considerations of Image Analysis and Quantification
of Signal Transduction IHC Staining.................................. 143
Michael Grunkin, Jakob Raundahl, and Niels T. Foged
9 Flow Cytometric Analysis of Cell Signaling Proteins....................... 155
Maria A. Sunt and Vernon C. Maino
x Contents
10 CytoSys: A Tool for Extracting Cell-Cycle-Related Expression
Dynamics from Static Data.......................................... 171
Jayant Avva, Michael C. Weis, Radina P. Soebiyanto,
James W. Jacobberger, andSree N. Sreenath
Part IV Neuroscience, Cancer, and Stem Cell Research
11 Signaling Events Initiated by Kappa Opioid Receptor Activation:
Quantification and Immunocolocalization Using Phospho-Selective
KOR, P38 MAPK, and KIR 3.1 Antibodies.............................. 197
Julia C. Lemos, Clarisse A. Roth, and Charles Chavkin
12 Immunohistochemical Assessment of Signal Transduction
and Cell-Cycle Networks in Neural Tumors............................. 221
Daniel Ciznadija, Afiar Barlas, and Katia Manova
13 Novel Multicolor Immunofluorescence Technique
Using Primary Antibodies Raised in the Same Host Species................. 233
Jillian Frisch, J.P. Houchins, Michael Grahek, Jordan Schoephoerster,
Jodi Hagen, Joseph Sweet, Leopoldo Mendoza, David Schwartz,
and Alexander E. Kalyuzhny
14 Activation and Differentiation of Mesenchymal Stem Cells.................. 245
PravinJ. Mishra and Debabrata Banerjee
Part V Novel Assays and Techniques
15 Double In Situ Detection of Sonic Hedgehog mRNA
and pMAPK Protein in Examining the Cell Proliferation
Signaling Pathway in Mouse Embryo.................................. 257
Sho Fujisawa, Mesruh Turkekul, Afiar Barlas, Ning Fan,
and Katia Manova
16 Identifying Intracellular Sites of Eicosanoid Lipid Mediator
Synthesis with EicosaCell Assays...................................... 277
Christianne Bandeira-Melo, Peter F. Weller,
and Patricia T Bozza
17 Absorption Control in Immunohistochemistry Using Phospho- Peptides
Immobilized on Magnetic Beads...................................... 291
Jordan Schoephoerster, Jillian Frisch, Michael Grahek,
Chun Wu, Yingwei He, Wei Wang, Jennifer Nguyen,
David Schwartz, and Alexander E. Kalyuzhny
Index.................................................................. 301
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| id | DE-604.BV037392672 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T23:23:20Z |
| institution | BVB |
| isbn | 9781617790232 1617790230 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-022545526 |
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| owner_facet | DE-12 |
| physical | xiii, 302 p. ill. 27 cm |
| publishDate | 2011 |
| publishDateSearch | 2011 |
| publishDateSort | 2011 |
| publisher | Humana |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | Signal transduction immunohistochemistry methods and protocols ed. by Alexander E. Kalyuzhny New York, NY Humana 2011 xiii, 302 p. ill. 27 cm txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 717 Includes bibliographical references and index Cellular signal transduction Immunohistochemistry Signal Transduction Kaljužnyj, Aleksandr Sonstige oth Methods in molecular biology 717 (DE-604)BV035362695 717 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022545526&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Signal transduction immunohistochemistry methods and protocols Methods in molecular biology Cellular signal transduction Immunohistochemistry Signal Transduction |
| title | Signal transduction immunohistochemistry methods and protocols |
| title_auth | Signal transduction immunohistochemistry methods and protocols |
| title_exact_search | Signal transduction immunohistochemistry methods and protocols |
| title_full | Signal transduction immunohistochemistry methods and protocols ed. by Alexander E. Kalyuzhny |
| title_fullStr | Signal transduction immunohistochemistry methods and protocols ed. by Alexander E. Kalyuzhny |
| title_full_unstemmed | Signal transduction immunohistochemistry methods and protocols ed. by Alexander E. Kalyuzhny |
| title_short | Signal transduction immunohistochemistry |
| title_sort | signal transduction immunohistochemistry methods and protocols |
| title_sub | methods and protocols |
| topic | Cellular signal transduction Immunohistochemistry Signal Transduction |
| topic_facet | Cellular signal transduction Immunohistochemistry Signal Transduction |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022545526&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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