RNA detection and visualization: methods and protocols
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York [u.a.]
Humana
2011
|
| Schriftenreihe: | Methods in molecular biology
714 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Includes bibliographical references and index |
| Beschreibung: | XIV, 497 S. Ill., graph. Darst. 27 cm |
| ISBN: | 9781617790041 |
Internformat
MARC
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| 245 | 1 | 0 | |a RNA detection and visualization |b methods and protocols |c ed. by Jeffrey E. Gerst |
| 264 | 1 | |a New York [u.a.] |b Humana |c 2011 | |
| 300 | |a XIV, 497 S. |b Ill., graph. Darst. |c 27 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 714 | |
| 500 | |a Includes bibliographical references and index | ||
| 650 | 4 | |a RNA / Analysis / Laboratory manuals | |
| 650 | 4 | |a RNA / analysis / Laboratory Manuals | |
| 650 | 4 | |a Genetic Techniques / Laboratory Manuals | |
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Datensatz im Suchindex
| _version_ | 1804145678323023872 |
|---|---|
| adam_text | Contents
Preface
.................................................................
v
Contributors
............................................................. xi
Part I Visualizing mRNAs In Situ
1
Single Molecule Imaging of
RNA
In Situ
............................... 3
Mona
Batish, Arjun Raj, and Sanjay
Ту
agi
2
Б1ЅН
and Immunofluorescence Staining in Chlamydomonas
................. 15
James Uniacke,
Daniét Colón-Ramos,
and William Zerges
3
High Resolution Fluorescent In Situ Hybridization in
Drosophila
............. 31
Eric
Lécnyer
4
Localization and Anchorage of Maternal mRNAs to Cortical Structures
of Ascidian Eggs and Embryos Using High Resolution In Situ Hybridization
.... 49
Alexandre Paix,
Janet Chenevert, and Christian Sardet
5
Visualization of mRNA Localization in Xenopus Oocytes
................... 71
James A. Gagnon and Kimberly L. Mowry
6
Visualization of mRNA Expression in the Zebrafish Embryo
................. 83
Yossy Machlufand Gil Levkowitz
7
High-Resolution Fluorescence In Situ Hybridization to Detect mRNAs
in
Neuronal
Compartments In Vitro and In Vivo
......................... 103
Sharon A. Swanger, Gary
J
.
Bassell, and Christina Gross
8
Localization of mRNA in Vertebrate Axonal Compartments
by In Situ Hybridization
............................................ 125
José
Roberto Sotelo-Silveira,
Aldo
Calliari,
Alejandra
Кип,
Victoria Elizondo,
Lucia
Canclini, and
Josá
Roberto Sotelo
Part II Visualizing mRNAs In Vivo Using Molecular
Probes or Reconstituted Fluorescent Proteins
9
Tiny Molecular Beacons for in vivo mRNA Detection
...................... 141
Diana P.
Brat», Irina
R
Catrina,
and
Salvatore
A.B.
Harnu
10
Delivery of Molecular Beacons for Live-Cell Imaging and Analysis of
RNA
...... 159
Antony
К.
Chen, Won Jong Rhee, Gang Boo, and Andrew
Тѕоигклѕ
11
Genetically-Encoded Fluorescent Probes for Imaging Endogenous
mRNA in Living Cells
............................................. 175
Такеакі
Ozawa and Yosbio Umezawa
12
Visualization of Induced
RNA
in Single Bacterial Cells
..................... 189
Aura Borogovac and Natalia
R
Brande
VÜ
viii Contents
Part III Visualizing mKNAs In Vivo Using Aptamers and Intact
Fluorescent Proteins
13
Visualizing mRNAs in Fixed and Living Yeast Cells
........................ 203
Franck
Gallardo
and Pascal
Chartrand
14
In Vivo Visualization of
RNA
Using the UlA-Based Tagged
RNA
System
...... 221
Sunglan Chung and Peter A. Takizawa
15
Visualizing Endogenous mRNAs in Living Yeast Using m-TAG, a PCR-Based
RNA
Aptamer Integration Method, and Fluorescence Microscopy
............ 237
lAora Haim-Vilmovsky and Jeffrey
E. Gerst
16
Imaging mRNAs in Living Mammalian Cells
............................ 249
Sharon Yunger and Yaron Sbav-Tal
17
Using the
mRNA-MS2/MS2CP-FP
System to Study mRNA
Transport During
Drosophila
Oogénesis................................
265
Katsiaryna
Belaya and Daniel St Johnston
Part IV Use of Cell Fractionation to Demonstrate
the Sub-Cellular Localization of
RNA
18
Genome-Wide Analysis of
RNA
Extracted from Isolated Mitochondria
......... 287
Erez Eliyahu,
Daniel Melamed, and Yoav
Arava
19
Analyzing mRNA Localization to the Endoplasmic Reticulum via
Cell Fractionation
................................................. 301
Sujatha Jagannathan, Christine Nwosu, and Christopher V.
Nicchiila
20
Isolation of mRNAs Encoding Peroxisomal Proteins from Yeast
Using a Combined Cell Fractionation and Affinity Purification Procedure
....... 323
Gadi
Zapor,
Cecile
Brocard, and Jeffrey
E. Gerst
21
Profiling
Axonal mRNA Transport
.................................... 335
DiannaE. Willis and JefferyL· Twiss
11
RNA
Purification from
Tumor
Celi
Protrusions Using Porous
Polycarbonate Filters
.............................................. 353
Jay Sbankar and Ivan
Ä. Nabi
Part V Affinity Purification of mRNAs and the Identification
of Trans-Acting Factors
23
RNA-Binding Protein
Immunopurificatìon-Microarray (RlP-Chip)
Analysis to Profile Localized RNAs
.................................... 369
Alesna
Galgana
and
André P.
Gerber
24
RaPID: An Aptamer-Based mRNA Affinity Purification Technique
for the Identification of
RNA
and Protein Factors Present
in Ribonudeoprotein Complexes
..................................... 387
Boris Slobodin and Jeffrey
R
Gerst
25
RIP: An mRNA Localization Technique
................................ 407
Sabarinath Jayaseelan, Francis Doyle,
Salvatore
Current*,
and Scott A. Tenenbaum
Contents ix
26
The Dual Use of
RNA
Aptamer Sequences for Affinity Purification
and Localization Studies of RNAs and RNA-Protein Complexes
.............. 423
ScottC. Walker, Paul
D
.
Good, Theresa A. Gipon, and David R. Engelke
Part VI Use of Bioinformatics to Identify
Cis-
Acting Motifs
and Structures in RNAs
27
Identifying and Searching for Conserved
RNA
Localisation Signals
............ 447
Russell S. Hamilton and
lian
Davis
28
Computational Prediction of
RNA
Structural Motifs Involved
in Post-Transcriptional Regulatory Processes
............................. 467
Michal
Rąbani,
Michael
Kertész,
and
Eran
Segal
Index
.................................................................. 481
|
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| id | DE-604.BV037389684 |
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| indexdate | 2024-07-09T23:23:16Z |
| institution | BVB |
| isbn | 9781617790041 |
| language | English |
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| spelling | RNA detection and visualization methods and protocols ed. by Jeffrey E. Gerst New York [u.a.] Humana 2011 XIV, 497 S. Ill., graph. Darst. 27 cm txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 714 Includes bibliographical references and index RNA / Analysis / Laboratory manuals RNA / analysis / Laboratory Manuals Genetic Techniques / Laboratory Manuals Messenger-RNS (DE-588)4169499-5 gnd rswk-swf Biochemische Analyse (DE-588)4255721-5 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf RNS (DE-588)4076759-0 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content RNS (DE-588)4076759-0 s Biochemische Analyse (DE-588)4255721-5 s Methode (DE-588)4038971-6 s DE-604 Messenger-RNS (DE-588)4169499-5 s b DE-604 Gerst, Jeffrey E. edt Erscheint auch als Online-Ausgabe 978-1-61779-005-8 Methods in molecular biology 714 (DE-604)BV035362695 714 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022542600&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | RNA detection and visualization methods and protocols Methods in molecular biology RNA / Analysis / Laboratory manuals RNA / analysis / Laboratory Manuals Genetic Techniques / Laboratory Manuals Messenger-RNS (DE-588)4169499-5 gnd Biochemische Analyse (DE-588)4255721-5 gnd Methode (DE-588)4038971-6 gnd RNS (DE-588)4076759-0 gnd |
| subject_GND | (DE-588)4169499-5 (DE-588)4255721-5 (DE-588)4038971-6 (DE-588)4076759-0 (DE-588)4143413-4 |
| title | RNA detection and visualization methods and protocols |
| title_auth | RNA detection and visualization methods and protocols |
| title_exact_search | RNA detection and visualization methods and protocols |
| title_full | RNA detection and visualization methods and protocols ed. by Jeffrey E. Gerst |
| title_fullStr | RNA detection and visualization methods and protocols ed. by Jeffrey E. Gerst |
| title_full_unstemmed | RNA detection and visualization methods and protocols ed. by Jeffrey E. Gerst |
| title_short | RNA detection and visualization |
| title_sort | rna detection and visualization methods and protocols |
| title_sub | methods and protocols |
| topic | RNA / Analysis / Laboratory manuals RNA / analysis / Laboratory Manuals Genetic Techniques / Laboratory Manuals Messenger-RNS (DE-588)4169499-5 gnd Biochemische Analyse (DE-588)4255721-5 gnd Methode (DE-588)4038971-6 gnd RNS (DE-588)4076759-0 gnd |
| topic_facet | RNA / Analysis / Laboratory manuals RNA / analysis / Laboratory Manuals Genetic Techniques / Laboratory Manuals Messenger-RNS Biochemische Analyse Methode RNS Aufsatzsammlung |
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