Genetic cell lineage tracing and transcriptome analysis of neuronal progenitor cells during neurogenesis and regeneration of the adult zebrafish telencephalon:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2010
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 83 Bl. Ill., graph. Darst. |
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| 245 | 1 | 0 | |a Genetic cell lineage tracing and transcriptome analysis of neuronal progenitor cells during neurogenesis and regeneration of the adult zebrafish telencephalon |c von Dorian Freudenreich |
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| adam_text | IMAGE 1
GENETIC CELL LINEAGE TRACING AND TRANSCRIPTOME ANALYSIS
OF NEURONAL PROGENITOR CELLS DURING NEUROGENESIS AND REGENERATION OF THE
ADULT ZEBRAFISH TELENCEPHALON
DISSERTATION
ZUR ERLANGUNG DES AKADEMISCHEN GRADES DOCTOR RERUM NATURALIUM (DR. RER.
NAT.)
VORGELEGT DER
FAKULTAET MATHEMATIK UND NATURWISSENSCHAFTEN DER TECHNISCHEN UNIVERSITAET
DRESDEN VON
DORIAN FREUDENREICH
GEBOREN AM 17. MAERZ 1981 IN FREIBURG IM BREISGAU
GUTACHTER: PROF. DR. MICHAEL BRAND PROF. DR. GERD KEMPERMANN
EINGEREICHT AM: 03.05.2010 TAG DER VERTEIDIGUNG: 24.06.2010
BIBLIOGRAFISCHE INFORMATIONEN HTTP://D-NB.INFO/1008152196
DIGITALISIERT DURCH
IMAGE 2
TABLE OF CONTENTS
TABLE OF CONTENTS
LIST OF PUBLICATIONS
SUMMARY
INTRODUCTION
ADULT NEUROGENESIS IN MAMMALS 8
- NEUROGENESIS IN THE HIPPOCAMPUS OF RODENTS 9
- NEUROGENESIS IN THE SUBVENTRICULAR ZONE OF RODENTS 9
ADULT NEUROGENESIS IN ZEBRAFISH 10
FUNCTIONS OF ADULT NEUROGENESIS 12
REGULATION OF ADULT NEUROGENESIS - OVERVIEW 13
- NOTCH SIGNALLING AND NEUROGENESIS 14
- NOTCH SIGNALLING IN THE POSTNATAL AND ADULT BRAIN 15
NEURODEGENERATION, BRAIN INJURY AND REGENERATION 16 GENOME WIDE
APPROACHES TO IDENTIFY REGULATORS OF NEUROGENESIS 16 AND REGENERATION
ZEBRAFISH AS A MODEL ORGANISM 17
CRE/LOX SYSTEM FOR GENETIC FATE MAPPING 17
MATERIALS AND METHODS
MATERIALS - TECHNICAL EQUIPMENT 19
- TRANSGENIC LINES 19
- KITS 19
- REAGENTS AND BUFFERS 20
- PRIMARY ANTIBODIES 21
- SECONDARY ANTIBODIES 21
- IN SITU HYBRIDISATION PROBES 22
METHODS - FISH MAINTENANCE 23
- DISSOCIATION OF BRAINS 23
- CELL CULTURE CONDITIONS 23
- DAPT TREATMENT 23
- BRDU LABELLING 24
- PREPARATION OF CRYOSECTIONS 24
- IMMUNOHISTOCHEMISTRY 24
IMAGE 3
TABLE OF CONTENTS
IN SITU HYBRIDISATION AND PROBE SYNTHESIS 25
IMAGE ACQUISITION AND PROCESSING 26
GENERATION OF TRANSGENIC LINES 27
TAMOXIFEN TREATMENTS 27
HEAT SHOCK EXPERIMENTS 27
BRAIN LESIONS 28
ELECTRON MICROSCOPY AND PREPARATION OF SECTIONS 28
FLOW CYTOMETRY, CELL SORTING AND RNA PURIFICATION 28
GENE EXPRESSION ARRAY 29
BIOINFORMATIC ANALYSIS 30
AIMS OF THIS STUDY 31
RESULTS
HER4.1 IN THE ADULT ZEBRAFISH BRAIN - EXPRESSION OF HER4.1 IN RADIAL
GLIA CELLS OF THE ADULT ZEBRAFISH 32 TELENCEPHALON - HER4.1 IS A TARGET
GENE OF NOTCH SIGNALLING IN THE ADULT BRAIN 33 - EPENDYMAL AND RADIAL
GLIA CHARACTERISTICS OF HER4.1 CELLS 34
- CHARACTERISATION OF VENTRICULAR PROLIFERATING CELLS INDEPENDENT OF 36
GLIAL MARKERS - HER4.1 CELLS SHOW PROGENITOR CHARACTERISTICS 36
GENETIC FATE MAPPING - HER4.1 CELLS GENERATE NEW NEURONS 37
- NEURONAL INTEGRATION AND SUBTYPE SPECIFICATION 40
- HER4.1 CELLS: INTERMEDIATE PROGENITOR CELLS? 41
- MOLECULAR MECHANISMS UNDERLYING NEUROGENESIS 42
- HER4.1 CELLS GENERATE NEW NEURONS AFTER INJURY OF THE ADULT BRAIN 43
MICROARRAY - MICROARRAY ANALYSIS 45
- FIRST VERIFICATION OF MICROARRAY RESULTS 47
- DIFFERENTIALLY REGULATED GENE EXPRESSION IN NEUROGENESIS 48 -
DIFFERENTIALLY REGULATED GENE EXPRESSION IN REGENERATION 48 - MICROARRAY
DATA PROCESSING BY INTEGRATION OF SEVERAL CRITERIA 51 - COMPARISON OF
TWO DIFFERENT METHODS FOR GENE IDENTIFICATION 52 - REGENERATION SPECIFIC
GENES 53
- BIOINFORMATIC ANALYSIS 55
- KEGG PATHWAY ANALYSIS - NEUROGENESIS RELATED PATHWAYS 55 - GENE
ONTOLOGY ANALYSIS - REGENERATION RELATED GENES AND PATHWAYS 56
IMAGE 4
TABLE OF CONTENTS
DISCUSSION
NEURONAL PROGENITOR CELLS IN THE ADULT ZEBRAFISH TELENCEPHALON 57 HER4.1
CELL CHARACTERISTICS 58
NEUROGENIC AND NON-NEUROGENIC GLIA CELLS 58
HER4.1 AND CELL PROLIFERATION 58
GENETIC FATE MAPPING OF HER4.1 CELLS - INDUCTION OF RECOMBINATION 59
- HER4.1 DRIVEN CREER 72 EXPRESSION IN RETINA AND SPINAL CORD 60 -
HER4.1 CELLS GENERATE NEURONS 60
- FATE MAPPING FROM EMBRYO TO ADULT 61
- NOTCH SIGNALLING IN NEUROGENESIS 62
- ACTIVATION OF THE DELTA/NOTCH PATHWAY AFTER INJURY 62
MICROARRAY ANALYSIS - MICROARRAY RESULTS 63
- GENES INVOLVED IN NEUROGENESIS 63
- GENES INVOLVED IN REGENERATION 63
- REGENERATION SPECIFIC GENES 65
- MICROARRAY DATA ANALYSIS 65
- KEGG AND GO ANALYSIS 66
CONCLUSIONS AND PERSPECTIVES 68
APPENDIX 69
REFERENCES 70
ABBREVIATIONS 80
ACKNOWLEDGEMENTS 82
DECLARATION 83
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| discipline | Biologie |
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| spelling | Freudenreich, Dorian 1981- Verfasser (DE-588)142017264 aut Genetic cell lineage tracing and transcriptome analysis of neuronal progenitor cells during neurogenesis and regeneration of the adult zebrafish telencephalon von Dorian Freudenreich 2010 83 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Dresden, Tech. Univ., Diss., 2010 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022482270&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Freudenreich, Dorian 1981- Genetic cell lineage tracing and transcriptome analysis of neuronal progenitor cells during neurogenesis and regeneration of the adult zebrafish telencephalon |
| subject_GND | (DE-588)4113937-9 |
| title | Genetic cell lineage tracing and transcriptome analysis of neuronal progenitor cells during neurogenesis and regeneration of the adult zebrafish telencephalon |
| title_auth | Genetic cell lineage tracing and transcriptome analysis of neuronal progenitor cells during neurogenesis and regeneration of the adult zebrafish telencephalon |
| title_exact_search | Genetic cell lineage tracing and transcriptome analysis of neuronal progenitor cells during neurogenesis and regeneration of the adult zebrafish telencephalon |
| title_full | Genetic cell lineage tracing and transcriptome analysis of neuronal progenitor cells during neurogenesis and regeneration of the adult zebrafish telencephalon von Dorian Freudenreich |
| title_fullStr | Genetic cell lineage tracing and transcriptome analysis of neuronal progenitor cells during neurogenesis and regeneration of the adult zebrafish telencephalon von Dorian Freudenreich |
| title_full_unstemmed | Genetic cell lineage tracing and transcriptome analysis of neuronal progenitor cells during neurogenesis and regeneration of the adult zebrafish telencephalon von Dorian Freudenreich |
| title_short | Genetic cell lineage tracing and transcriptome analysis of neuronal progenitor cells during neurogenesis and regeneration of the adult zebrafish telencephalon |
| title_sort | genetic cell lineage tracing and transcriptome analysis of neuronal progenitor cells during neurogenesis and regeneration of the adult zebrafish telencephalon |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022482270&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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