The type IV secretion genomic island of Neisseria gonorrhoeae and its impact on human cells:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Berlin
Mensch und Buch Verl.
2011
|
| Schriftenreihe: | Biological science
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | VIII, 102 S. Ill., graph. Darst. |
| ISBN: | 9783866649361 |
Internformat
MARC
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Datensatz im Suchindex
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| adam_text | IMAGE 1
TABLE OF
CONTENTS
TABLE OF CONTENTS
ZUSAMMENFASSUNG I
ABSTRACT ILL
1. INTRODUCTION 1
1.1 NEISSERIA GONORRHOEAE 1
1.1.1 NEISSERIA SPP 1
1.1.2 STRUCTURE AND CLINICAL MANIFESTATIONS 1
1.2 GENETIC FEATURES OF NEISSERIA GONORRHOEAE 2
1.2.1 TRANSFORMATION IN NEISSERIA GONORRHOEAE 2
1.2.2 TYPE IV SECRETION SYSTEMS 4
1.2.3 GONOCOCCAL GENETIC ISLAND 6
1.3 PATHOGENICITY FACTORS OF NEISSERIA GONORRHOEAE AND HOST INTERACTIONS
9
1.3.1 TYPE IV PILI 9
1.3.2 OPA PROTEINS 10
1.3.3 LIPOOLIGOSACCHARIDE (LOS) 11
1.3.4 PORINS 12
1.3.5 INTERACTIONS WITH THE HOST 13
1.3.6 HOST RESPONSE IS
2. OBJECTIVES 25
3. NUCLEIC ACID SECRETION BY NEISSERIA GONORRHOEAE 26
3.1 A SHORT INTRODUCTION 26
BIBLIOGRAFISCHE INFORMATIONEN HTTP://D-NB.INFO/1010964313
DIGITALISIERT DURCH
IMAGE 2
TABLE OF
CONTENTS
3.2 RESULTS 27
3.2.1 GENERATION AND CHARACTERIZATION OF GGI MUTANTS 27
3.2.3 NUCLEIC ACID RELEASE FROM MS11 WILD TYPE, MSLLTRAG AND MS11GGI 32
3.3 DISCUSSION 37
K INFLUENCE OF THE GONOCOCCAL GENETIC ISLAND ON THE HOST 40
4.1 A SHORT INTRODUCTION 40
4.2 RESULTS 42
4.2.1 IL-8 RESPONSE TO NEISSERIA GONORRHOEAE 42
4.2.2 MAPKS AND NF-KB ACTIVATION AFTER HIGH BACTERIAL LOAD INFECTIONS 44
4.2.3 APOPTOSIS REGULATION BY NEISSERIA GONORRHOEAE INFECTION 46
4.2.6 MAPK ACTIVATION AFTER INFECTION WITH LOW BACTERIAL LOAD AND ERK1/2
MOI DEPENDENCY
54
4.3 DISCUSSION 57
I. CONCLUSION AND OUTLOOK 61
I. MATERIALS 64
6.1 CELL LINES AND MEDIA 64
6.2 BACTERIAL STRAINS 64
6.3 OLIGONUDEOTIDES 65
6.4 VECTORS 65
6.5 ANTIBODIES 66
6.5.1 PRIMARY ANTIBODIES 66
6.5.2 SECONDARY ANTIBODIES 66
VI
IMAGE 3
TABLE OF
CONTENTS
6.6 ANTIBIOTICS 67
6.7 ENZYMES 67
6.8 MEDIA FOR BACTERIA 67
6.9 BUFFERS AND SOLUTIONS 68
6.9.1 SDS-PAGE AND WESTERN BLOT 68
6.9.2 IMMUNOFLUORESCENCE 69
6.9.3 CLONING 70
6.9.4 IL-8 ELISA 70
6.10 CHEMICAL REAGENTS 71
6.11 KITS 71
6.12 LABWARE 72
6.13 INSTRUMENTS 72
6.13 SOFTWARE 73
7. METHODS 74
7.1 CELL CULTURE METHODS 74
7.1.1 PASSAGING OF CELLS 74
7.1.2 INFECTION OF CELLS WITH N. GONORRHOEAE 74
7.1.3 ADHESION ASSAY 75
7.1.4 APOPTOSIS ASSAY 75
7.2 GROWTH AND MANIPULATION OF BACTERIA 76
7.2.1 GROWTH OF W. GONORRHOEAE AND E. COLI 76
7.2.2 TRANSFORMATION OF E. COLI 76
VII
IMAGE 4
TABLE OF
CONTENTS
7.2.3 TRANSFORMATION OF N. GONORRHOEAE 77
7.3 NUCLEIC ACID METHODS 77
7.3.1 ISOLATION OF GENOMIC DNA FROM N. GONORRHOEAE 77
7.3.2 CLONING OF TRANSFORMATION VECTORS 77
7.4 PROTEIN BIOCHEMICAL METHODS 79
7.4.1 DISCONTINUOUS SDS POLYACRYLAMID GEL ELECTROPHORESIS (SDS-PAGE) 79
7.4.2 IMMUNOBLOT (WESTERN BLOT) 79
7.4.3 IL-8 ELISA 80
7.5 MICROSCOPY 80
7.5.1 INDIRECT IMMUNOFLUORESCENCE STAINING 80
7.5.2 CONFOCAL LASER SCANNING MICROSCOPY 81
7.6 STATISTICS 81
8. LIST OF ABBREVIATIONS 82
9. REFERENCES 85
10. APPENDIX 96
10.1 SUPPLEMENTARY FIGURES 96
10.2 PUBLICATIONS 101
10.3 SELBSTAENDIGKEITSERKLAERUNG 102
VIII
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| isbn | 9783866649361 |
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| spelling | Munke, Rebekka 1977- Verfasser (DE-588)143856030 aut The type IV secretion genomic island of Neisseria gonorrhoeae and its impact on human cells von Rebekka Munke Berlin Mensch und Buch Verl. 2011 VIII, 102 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Biological science Zugl.: Berlin, Humboldt-Univ., Diss., 2011 Neisseria gonorrhoeae (DE-588)4278906-0 gnd rswk-swf Pathogenität (DE-588)4205990-2 gnd rswk-swf Gen (DE-588)4128987-0 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Neisseria gonorrhoeae (DE-588)4278906-0 s Pathogenität (DE-588)4205990-2 s Gen (DE-588)4128987-0 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=022458745&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Munke, Rebekka 1977- The type IV secretion genomic island of Neisseria gonorrhoeae and its impact on human cells Neisseria gonorrhoeae (DE-588)4278906-0 gnd Pathogenität (DE-588)4205990-2 gnd Gen (DE-588)4128987-0 gnd |
| subject_GND | (DE-588)4278906-0 (DE-588)4205990-2 (DE-588)4128987-0 (DE-588)4113937-9 |
| title | The type IV secretion genomic island of Neisseria gonorrhoeae and its impact on human cells |
| title_auth | The type IV secretion genomic island of Neisseria gonorrhoeae and its impact on human cells |
| title_exact_search | The type IV secretion genomic island of Neisseria gonorrhoeae and its impact on human cells |
| title_full | The type IV secretion genomic island of Neisseria gonorrhoeae and its impact on human cells von Rebekka Munke |
| title_fullStr | The type IV secretion genomic island of Neisseria gonorrhoeae and its impact on human cells von Rebekka Munke |
| title_full_unstemmed | The type IV secretion genomic island of Neisseria gonorrhoeae and its impact on human cells von Rebekka Munke |
| title_short | The type IV secretion genomic island of Neisseria gonorrhoeae and its impact on human cells |
| title_sort | the type iv secretion genomic island of neisseria gonorrhoeae and its impact on human cells |
| topic | Neisseria gonorrhoeae (DE-588)4278906-0 gnd Pathogenität (DE-588)4205990-2 gnd Gen (DE-588)4128987-0 gnd |
| topic_facet | Neisseria gonorrhoeae Pathogenität Gen Hochschulschrift |
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