The function of Krüppel-like factor in B-cell development: = Die Funktion von Krüppel-like Faktor 2 in der B-Zellentwicklung
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2010
|
| Schlagworte: | |
| Online-Zugang: | kostenfrei https://nbn-resolving.org/urn:nbn:de:bvb:29-opus-22762 http://d-nb.info/1010641980/34 Inhaltsverzeichnis |
| Beschreibung: | V, 93 S. Ill., graph. Darst. 21 cm |
Internformat
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV037256973 | ||
| 003 | DE-604 | ||
| 005 | 20110325 | ||
| 007 | t | ||
| 008 | 110302s2010 ad|| m||| 00||| eng d | ||
| 016 | 7 | |a 1010033700 |2 DE-101 | |
| 035 | |a (OCoLC)711841613 | ||
| 035 | |a (DE-599)DNB1010033700 | ||
| 040 | |a DE-604 |b ger |e rakddb | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-384 |a DE-473 |a DE-703 |a DE-1051 |a DE-824 |a DE-29 |a DE-12 |a DE-91 |a DE-19 |a DE-1049 |a DE-92 |a DE-739 |a DE-898 |a DE-355 |a DE-706 |a DE-20 |a DE-1102 |a DE-29T | ||
| 084 | |a 610 |2 sdnb | ||
| 100 | 1 | |a Winkelmann, Rebecca |d 1982- |e Verfasser |0 (DE-588)143505572 |4 aut | |
| 245 | 1 | 0 | |a The function of Krüppel-like factor in B-cell development |b = Die Funktion von Krüppel-like Faktor 2 in der B-Zellentwicklung |c vorgelegt von Rebecca Winkelmann, geb. Heidbüchel |
| 246 | 1 | 1 | |a Die Funktion von Krüppel-like Faktor 2 in der B-Zellentwicklung |
| 264 | 1 | |c 2010 | |
| 300 | |a V, 93 S. |b Ill., graph. Darst. |c 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Erlangen-Nürnberg, Univ., Diss., 2010 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 776 | 0 | 8 | |i Erscheint auch als |n Online-Ausgabe |o urn:nbn:de:bvb:29-opus-22762 |
| 856 | 4 | |u https://open.fau.de/handle/openfau/1559 |x Verlag |z kostenfrei |3 Volltext | |
| 856 | 4 | |u https://nbn-resolving.org/urn:nbn:de:bvb:29-opus-22762 |x Resolvingsystem | |
| 856 | 4 | |u http://d-nb.info/1010641980/34 |x Langzeitarchivierung Nationalbibliothek | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=021170186&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 912 | |a ebook | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-021170186 | ||
Datensatz im Suchindex
| _version_ | 1804143874612920320 |
|---|---|
| adam_text | TABLE
OF
CONTENTS
1
ZUSAMMENFASSUNG
...................................................................................
1
2
SUMMARY
....................................................................................................
2
3
INTRODUCTION
................................................................................................
3
3.1
B
CELL
DEVELOPMENT
AND
HOMEOSTASIS
...................................................................
3
3.1.1
CENTRAL
B
CELL
DEVELOPMENT
...................................................................................
3
3.1.2
PERIPHERAL
B
CELL
DEVELOPMENT
..............................................................................
6
3.1.3
PLASMA
CELL
GENERATION
AND
MIGRATION
...................................................................
9
3.2
KRUPPEL-LIKE
FACTOR
2
(KLF2)
...............................................................................
11
3.2.1
KRUPPEL-LIKE
TRANSCRIPTION
FACTORS
........................................................................
11
3.2.2
KLF2
IN
T
LYMPHOCYTES
........................................................................................
12
3.2.3
REGULATION
OF
KLF2
..............................................................................................
13
4
SPECIFIC
AIMS
.........................................................................................
15
5
RESULTS
.....................................................................................................
16
5.1
B
CELL
SPEFICIC
KLF2
DELETION
IN
VIVO
........................................................................
16
5.1.1
KLF2
EXPRESSION
PROFILE
DURING
B
CELL
DEVELOPMENT
..........................................
16
5.1.2
NORMAL
B
CELL
DEVELOPMENT
IN
THE
BONE
MARROW
OF
KLF2-DEFICIENT
ANIMALS
......
18
5.1.3
ENLARGED
SPLEEN
SIZE
AND
INCREASED
NUMBERS
OF
SPLENIC
B
CELL
POPULATIONS
IN
KLF2-DEFICIENT
ANIMALS
..................................................................................
20
5.1.4
DECREASED
FREQUENCIES
OF
B
CELLS
IN
THE
PERITONEAL
CAVITY,
THE
BLOOD
AND
PEYERS
PATCHES
OF
KLF2-DEFICIENT
MICE
.............................................................
25
5.1.5
REDUCED
NUMBERS
OF
PLASMA
CELLS
IN
THE
BONE
MARROW
AFTER
BOOST
IMMUNIZATION
WITH
TD
ANTIGEN
............................................................................
28
5.1.6
DISTRIBUTION
OF
PERIPHERAL
B
CELL
SUBSETS
IS
CONTROLLED
BY
KLF2
REGULATING
L-SELECTIN
AND
A47
INTEGRIN
BUT
NOT
S1P1
..........................................................
32
5.1.7
KLF2
DETERMINES
FO
B
CELL
IDENTITY
.....................................................................
35
5.2
BIOCHEMICAL
ANALYSES
OF
KLF2
...........................................................................
37
5.2.1
KLF2
PROTEIN
MODIFICATIONS
IN
SPLENIC
B
CELLS
AND
NON
B
CELLS
...........................
37
5.2.2
IN
SILICO
PHOSPHORYLATION
ANALYSIS
OF
KLF2
AMINO
ACID
SEQUENCE
......................
38
5.2.3
KLF2
IS
POSTTRANSLATIONALLY
MODIFIED
BY
PHOSPHORYLATION
...................................
40
I
6
DISCUSSION
...............................................................................................
42
6.1
KLF2-DEFICIENCY
RESULTS
IN
IMPAIRED
B
CELLS
HOMEOSTASIS
AND
PLASMA
CELL
HOMING
42
6.2
BIOCHEMICAL
ANALYSES
OF
KLF2
.............................................................................
47
7
MATERIAL
AND
METHODS
............................................................................
50
7.1
MATERIAL
................................................................................................................
50
7.1.1
CHEMICALS
...........................................................
50
7.1.2
ANTIBODIES
.........................................................................................................
50
7.1.3
PCR
PRIMER
........................................................................................................
53
7.1.4
PLASMIDS.............................................................................................................
54
7.1.5
CELL
LINES.............................................................................................................
55
7.1.6
MOUSE
STRAINS
....................................................................................................
55
7.1.7
BACTERIA
..............................................................................................................
56
7.1.8
TECHNICAL
EQUIPMENT
.........................................................................................
56
7.1.9
PLASTIC
MATERIAL
..................................................................................................
57
7.1.10
CONSUMABLE
MATERIAL
.........................................................................................
58
7.2
METHODS
...............................................................................................................
59
7.2.1
CELL
CULTURE
.........................................................................................................
59
7.2.1.1
CULTIVATION
AND
HARVEST
OF
VERTEBRATE
CELL
LINES
..................................................
59
7.2.1.2
CRYOCONSERVATION
..............................................................................................
59
7.2.1.3
THAWING
OF
CRYOCONSERVED
CELLS
........................................................................
59
7.2.1.4
CELL
COUNTING
.....................................................................................................
60
7.2.2
ISOLATION
OF
PRIMARY
B
AND
T
LYMPHOCYTES
FROM
DIFFERENT
ORGANS
.......................
60
7.2.2.1
ERYTHROCYTE
DEPLETION
.........................................................................................
60
7.2.2.2
MACS-SORTING
OF
CD43
SPLENIC
AND
CD19
+
BONE
MARROW
B
CELLS
....................
60
7.2.3
FLOW
CYTOMETRY
...................................................................................................
61
7.2.3.1
ANALYSES
OF
SURFACE
EXPRESSION
AND
INTRACELLULAR
PROTEINS................................
61
7.2.3.2
MOFLOYY
CELL
SORTING
...........................................................................................
61
7.2.4
HISTOLOGY
............................................................................................................
62
7.2.5
MOLECULAR
BIOLOGICAL
METHODS
............................................................................
62
7.2.5.1
PLASMID
DNA
AND
RNA
PREPARATION
..................................................................
62
7.2.5.2
DNA
RESTRICTION
ANALYSES
...................................................................................
62
7.2.5.3
DNA
FRAGMENT
AGAROSE
GEL
ELECTROPHORESIS
......................................................
63
II
7.2.5.4
ISOLATION
OF
GENOMIC
TAIL
DNA
...........................................................................
63
7.2.5.5
DNA/RNA
CONCENTRATION
...................................................................................
63
7.2.5.6
POLYMERASE
CHAIN
REACTION
(PCR)....................................................................
63
7.2.5.7 CDNA
SYNTHESIS
................................................................................................
64
7.2.5.8 QUALITATIVE
RT-PCR
..........................................................................................
64
7.2.5.9 QUANTITATIVE
TAQMAN
-RT-PCR
.......................................................................
65
7.2.5.10
AFFYMETRIX
MICROARRAY
ANALYSIS
.........................................................................
65
7.2.6
ANTIBODY
ISOTYPE
DETECTION
USING
ELISA
...........................................................
65
7.2.7
IGG
SPECIFIC
ELISPOT
...........................................................................................
66
7.2.8
PROTEINBIOCHEMISTRY
..........................................................................................
66
7.2.8.1
CELL
LYSATES
.......................................................................................................
66
7.2.8.2
NUCLEAR
EXTRACTS
...............................................................................................
66
7.2.8.3
BCA
TEST
............................................................................................................
67
7.2.8.4
PHOSPHATASE
INHIBITOR
AND
LY294002
STIMULATION
............................................
67
7.2.8.5
IMMUNOPRECIPITATION
.........................................................................................
67
7.2.8.6
SDS
PAGE
.......................................................................................................
68
7.2.8.7
WESTERN
BLOT
ANALYSIS
.......................................................................................
69
7.2.9
CALCIUM
MEASUREMENTS
....................................................................................
69
7.2.10
TRANSFECTION
AND
INFECTION
OF
VERTEBRATE
CELLS
...................................................
70
7.2.10.1
TRANSIENT
TRANSFECTION
OF
ADHERENT
CELLS
USING
CALCIUM
PHOSPHATE
..................
70
7.2.10.2
INFECTION
OF
NIH3T3
CELLS
..................................................................................
70
7.2.11
IN
VIVO
METHODS
................................................................................................
70
7.2.11.1
IMMUNIZATION
....................................................................................................
70
7.2.11.2
FTY720-TREATMENT
............................................................................................
71
7.2.11.3
BRDU-TREATMENT
.................................................................................................
71
7.2.12
STATISTICS
...........................................................................................................
71
8
REFERENCES
............................................................................................
72
9
ABBREVIATIONS
........................................................................................
87
10
PUBLICATIONS
...........................................................................................
91
11
VITA
..........................................................................................................
92
12
ACKNOWLEDGMENT
.................................................................................
93
III
|
| any_adam_object | 1 |
| author | Winkelmann, Rebecca 1982- |
| author_GND | (DE-588)143505572 |
| author_facet | Winkelmann, Rebecca 1982- |
| author_role | aut |
| author_sort | Winkelmann, Rebecca 1982- |
| author_variant | r w rw |
| building | Verbundindex |
| bvnumber | BV037256973 |
| collection | ebook |
| ctrlnum | (OCoLC)711841613 (DE-599)DNB1010033700 |
| discipline | Medizin |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV037256973 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T22:54:36Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-021170186 |
| oclc_num | 711841613 |
| open_access_boolean | 1 |
| owner | DE-384 DE-473 DE-BY-UBG DE-703 DE-1051 DE-824 DE-29 DE-12 DE-91 DE-BY-TUM DE-19 DE-BY-UBM DE-1049 DE-92 DE-739 DE-898 DE-BY-UBR DE-355 DE-BY-UBR DE-706 DE-20 DE-1102 DE-29T |
| owner_facet | DE-384 DE-473 DE-BY-UBG DE-703 DE-1051 DE-824 DE-29 DE-12 DE-91 DE-BY-TUM DE-19 DE-BY-UBM DE-1049 DE-92 DE-739 DE-898 DE-BY-UBR DE-355 DE-BY-UBR DE-706 DE-20 DE-1102 DE-29T |
| physical | V, 93 S. Ill., graph. Darst. 21 cm |
| psigel | ebook |
| publishDate | 2010 |
| publishDateSearch | 2010 |
| publishDateSort | 2010 |
| record_format | marc |
| spelling | Winkelmann, Rebecca 1982- Verfasser (DE-588)143505572 aut The function of Krüppel-like factor in B-cell development = Die Funktion von Krüppel-like Faktor 2 in der B-Zellentwicklung vorgelegt von Rebecca Winkelmann, geb. Heidbüchel Die Funktion von Krüppel-like Faktor 2 in der B-Zellentwicklung 2010 V, 93 S. Ill., graph. Darst. 21 cm txt rdacontent n rdamedia nc rdacarrier Erlangen-Nürnberg, Univ., Diss., 2010 (DE-588)4113937-9 Hochschulschrift gnd-content Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:29-opus-22762 https://open.fau.de/handle/openfau/1559 Verlag kostenfrei Volltext https://nbn-resolving.org/urn:nbn:de:bvb:29-opus-22762 Resolvingsystem http://d-nb.info/1010641980/34 Langzeitarchivierung Nationalbibliothek DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=021170186&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Winkelmann, Rebecca 1982- The function of Krüppel-like factor in B-cell development = Die Funktion von Krüppel-like Faktor 2 in der B-Zellentwicklung |
| subject_GND | (DE-588)4113937-9 |
| title | The function of Krüppel-like factor in B-cell development = Die Funktion von Krüppel-like Faktor 2 in der B-Zellentwicklung |
| title_alt | Die Funktion von Krüppel-like Faktor 2 in der B-Zellentwicklung |
| title_auth | The function of Krüppel-like factor in B-cell development = Die Funktion von Krüppel-like Faktor 2 in der B-Zellentwicklung |
| title_exact_search | The function of Krüppel-like factor in B-cell development = Die Funktion von Krüppel-like Faktor 2 in der B-Zellentwicklung |
| title_full | The function of Krüppel-like factor in B-cell development = Die Funktion von Krüppel-like Faktor 2 in der B-Zellentwicklung vorgelegt von Rebecca Winkelmann, geb. Heidbüchel |
| title_fullStr | The function of Krüppel-like factor in B-cell development = Die Funktion von Krüppel-like Faktor 2 in der B-Zellentwicklung vorgelegt von Rebecca Winkelmann, geb. Heidbüchel |
| title_full_unstemmed | The function of Krüppel-like factor in B-cell development = Die Funktion von Krüppel-like Faktor 2 in der B-Zellentwicklung vorgelegt von Rebecca Winkelmann, geb. Heidbüchel |
| title_short | The function of Krüppel-like factor in B-cell development |
| title_sort | the function of kruppel like factor in b cell development die funktion von kruppel like faktor 2 in der b zellentwicklung |
| title_sub | = Die Funktion von Krüppel-like Faktor 2 in der B-Zellentwicklung |
| topic_facet | Hochschulschrift |
| url | https://open.fau.de/handle/openfau/1559 https://nbn-resolving.org/urn:nbn:de:bvb:29-opus-22762 http://d-nb.info/1010641980/34 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=021170186&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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