Secondary growth in the Arabidopsis hypocotyl:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Göttingen
Cuvillier
2010
|
| Ausgabe: | 1. Aufl. |
| Schlagworte: | |
| Online-Zugang: | Inhaltstext Inhaltsverzeichnis |
| Beschreibung: | X, 112 S. Ill., graph. Darst. |
| ISBN: | 9783869555881 |
Internformat
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Datensatz im Suchindex
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| adam_text |
IMAGE 1
TABLE OF CONTENTS
LIST OF TABLES LIST OF FIGURES
LIST OF GENE ABBREVIATIONS
SUMMARY
1 INTRODUCTION 1
1.1 SECONDARY GROWTH IN PLANT STEMS AND THE ACTIVITY OF VASCULAR
CAMBIUM. 1
1.2 KNOX (KNOTTED-1 LIKE HOMEOBOX) GENE FUNCTION IN PLANT DEVELOPMENT. 2
1.3 MOLECULAR AND GENETIC CONTROL OF SECONDARY GROWTH, XYLEM
DIFFERENTIATION AND SECONDARY CELL WALL DEPOSITION 4
1.4 WORKING HYPOTHESIS 6
1.5 OBJECTIVES 7
2 MATERIALS AND METHODS 8
2.1 PLANT MATERIAL AND GROWTH CONDITIONS 8
2.2 IDENTIFICATION AND PRODUCTION OF HOMOZYGOUS SINGLE AND DOUBLE KNOX
MUTANTS 8
2.3 KNAT1-GR INDUCTION 11
2.4 RNA EXTRACTION AND CDNA SYNTHESIS 11
2.5 QUANTITATIVE REAL TIME RT-PCR 13
2.6 GUS ASSAYS 16
2.7 HISTOLOGIE ANALYSIS AND QUANTITATIVE MEASUREMENTS OF SECONDARY
GROWTH. 16
2.8 EMBEDDING AND TOLUIDINE BLUE STAINING 17
2.9 LIGHT MICROSCOPY 18
2.10 IDENTIFICATION OF GENES CO-EXPRESSED WITH STMANA KNAT1 18
BIBLIOGRAFISCHE INFORMATIONEN HTTP://D-NB.INFO/1009024264
DIGITALISIERT DURCH
IMAGE 2
3 RESULTS 19
3.1 ISOLATION OF INSERTIONAL ALIELES AS TOOL FOR FUNCTIONAL ANALYSES OF
ARABIDOPSIS KNOX GENES 19
3.2 DEFECTIVE SECONDARY XYLEM DEVELOPMENT IN ARABIDOPSIS HYPOCOTYLS STM
AND KNATL MUTANTS 22
3.3 STM AND KNAT1 ARE SPECIFICALLY EXPRESSED IN THE CAMBIAL ZONE OF
ARABIDOPSIS HYPOCOTYLS 27
3.4 THE DEVELOPMENT OF SECONDARY XYLEM IS INHIBITED IN STM AND KNATL
MUTANTS 28
3.5 PHASE II OF SECONDARY XYLEM DEVELOPMENT OI ARABIDOPSIS HYPOCOTYL IS
INHIBITED IN JIM AND KNATL MUTANTS 35
3.6 NON-EPISTATIC INTERACTION BETWEEN STM AND KNAT1 IN ARABIDOPSIS
HYPOCOTYLS 37
3.7 SECONDARY GROWTH OF ARABIDOPSIS HYPOCOTYLS WAS REDUCED BY
OVEREXPRESSION OF KNAT1 39
3.8 OVEREXPRESSION OF KNAT1 GENE DID NOT RESTORE THE STM PHENOTYPE IN
STM-GK 42
3.9 STM AND KNAT1 ARE REQUIRED FOR ATHB-8::GUS EXPRESSION IN PRECURSORS
OF XYLEM FIBERS 45
3.10 STMIKNAT1 ARE UPSTREAM OF GENES ASSOCIATED WITH VASCULAR MERISTEM
DIFFERENTIATION, FIBER DEVELOPMENT AND SECONDARY CELL WALL 46
4 DISCUSSION 54
4.1 INSERTIONAL MUTAGENESIS 54
4.2 SECONDARY GROWTH IN ARABIDOPSIS HYPOCOTYLS VERSUS INFLORESCENCE
STEMS 55
4.3 PLEIOTROPIC EFFECTS OF REDUCED STM AND KNAT1 GENE FUNCTION ON
SECONDARY GROWTH 56
4.4 GENETIC INTERACTION BETWEEN KNAT1 AND STM. 60
IMAGE 3
4.5 DOWNSTREAM FACTORS 61
4.6 OVEREXPRESSION CONTROVERSY 63
4.7 CONFLICT WITH PREVIOUSLY PUBLISHED DATA 64
4.8 WORKING MODEL OF ACTION OF STM AND KNA TI DURING SECONDARY XYLEM
DEVELOPMENT 65
5 REFERENCES 67
6 SUPPLEMENTAL DATA 74
7 APPENDIXES 92
PUBLICATION (SUNARYO AND FISCHER, 2009) 101
ACKNOWLEDGEMENTS 109
CURRICULUM VITAC 110 |
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| classification_tum | BIO 456d |
| ctrlnum | (OCoLC)706926358 (DE-599)DNB1009024264 |
| dewey-full | 571.862364 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 571 - Physiology & related subjects |
| dewey-raw | 571.862364 |
| dewey-search | 571.862364 |
| dewey-sort | 3571.862364 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| edition | 1. Aufl. |
| format | Thesis Book |
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| spelling | Sunaryo, Widi 1973- Verfasser (DE-588)143256211 aut Secondary growth in the Arabidopsis hypocotyl Widi Sunaryo 1. Aufl. Göttingen Cuvillier 2010 X, 112 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zugl.: Göttingen, Univ., Diss., 2010 Pflanzenwachstum (DE-588)4045595-6 gnd rswk-swf Hypokotyl (DE-588)4209535-9 gnd rswk-swf Ackerschmalwand (DE-588)4141299-0 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Ackerschmalwand (DE-588)4141299-0 s Hypokotyl (DE-588)4209535-9 s Pflanzenwachstum (DE-588)4045595-6 s DE-604 X:MVB text/html http://deposit.dnb.de/cgi-bin/dokserv?id=3635111&prov=M&dok_var=1&dok_ext=htm Inhaltstext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=021152619&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Sunaryo, Widi 1973- Secondary growth in the Arabidopsis hypocotyl Pflanzenwachstum (DE-588)4045595-6 gnd Hypokotyl (DE-588)4209535-9 gnd Ackerschmalwand (DE-588)4141299-0 gnd |
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| title | Secondary growth in the Arabidopsis hypocotyl |
| title_auth | Secondary growth in the Arabidopsis hypocotyl |
| title_exact_search | Secondary growth in the Arabidopsis hypocotyl |
| title_full | Secondary growth in the Arabidopsis hypocotyl Widi Sunaryo |
| title_fullStr | Secondary growth in the Arabidopsis hypocotyl Widi Sunaryo |
| title_full_unstemmed | Secondary growth in the Arabidopsis hypocotyl Widi Sunaryo |
| title_short | Secondary growth in the Arabidopsis hypocotyl |
| title_sort | secondary growth in the arabidopsis hypocotyl |
| topic | Pflanzenwachstum (DE-588)4045595-6 gnd Hypokotyl (DE-588)4209535-9 gnd Ackerschmalwand (DE-588)4141299-0 gnd |
| topic_facet | Pflanzenwachstum Hypokotyl Ackerschmalwand Hochschulschrift |
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