Verfügbarkeit: Antiviral RNAi

Antiviral RNAi: concepts, methods, and applications

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Bibliographische Detailangaben
Format:	Buch
Sprache:	English
Veröffentlicht:	New York, NY [u.a.] Humana Press 2011
Schriftenreihe:	Methods in molecular biology 721
Online-Zugang:	Inhaltsverzeichnis
Beschreibung:	XI, 411 S. Ill., graph. Darst.
ISBN:	1617790362 9781617790362

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Datensatz im Suchindex

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adam_text	Contents Preface ............................................................. v Contributors. ........................................................ ix Part I General Introduction 1 Defense and Counterdefense in the RNAi-Based Antiviral Immune System in Insects .......................................... 3 Joël T. van Mierlo, Koen W.R. van Chef, and Ronald P. van Rij 2 RNAi and Cellular miRNAs in Infections by Mammalian Viruses ............. 23 Joost Haasnoot and Ben Berkbout 3 Viral miRNAs .................................................... 43 Karlie Plaisance-Bonstaff and Rolf Renne 4 Progress in RNAi-Based Antiviral Therapeutics ........................... 67 Jiehua Zhou and John J. Rossi 5 Chemical Modification of Small Interfering RNA ......................... 77 Jesper В. Bramsen andj0rßen Kjems Part II Viral Small RNAs 6 Viral Small RNA Cloning and Sequencing .............................. 107 Valérie Gausson and Maria-Carla Saleh 7 Visitor, An Informatie Pipeline for Analysis of Viral siRNA Sequencing Datasets ..................................... 123 Christophe Antoniewski 8 Computational Prediction of Viral miRNAs ............................. 143 Adam Grundhoff 9 Detection of Viral microRNAs by Northern Blot Analysis ................... 153 Lydia V. McClure, Yao-Tang Lin, and Christopher S. Sullivan 10 Detection of Viral microRNA with SI Nuclease Protection Assay ............. 173 Matthias John and Sébastien Pfeffer 11 Characterization of RISC-Associated Adenoviral Small RNAs ................ 183 NingXu and Göran Akusßrvi Part III RNAi-Based Antiviral Defense 12 Identification of Viral Suppressors of RNAi by a Reporter Assay in Drosophila S2 Cell Culture .................................... 201 Koen W.R. van CleefiJoel T. van Mierlo, Marius van den Beek, and Ronald P. van Rij VI! viii Contents 13 Dicer Assay in Drosophila S2 Cell Extract ............................... 215 Baojun Yang and Hongwei Li 14 Slicer Activity in Drosophila melanągaster S2 Extract ....................... 231 Arabinda Nayak and Raul Andino 15 Gel Mobility Shift Assays for RNA Binding Viral RNAi Suppressors ........... 245 Tibor Csorba and József Burgyán 16 dsRNA Uptake in Adult Drosophila ................................... 253 Benjamin Obadia and Maria-Carla Saleh Part IV Antiviral RNAi Therapy 17 Design of Small Interfering RNAs for Antiviral Applications ................. 267 Diana Rothe, ErikJ. Wade, and Jens Kurreck 18 RNAi-Inducing Lentiviral Vectors for Anti-HIV-1 Gene Therapy ............. 293 Ting Poi Liuyjan-Tinus Westerink, Olivier ter Brake, and Ben Berkhout 19 Production of Multicopy shRNA Lentiviral Vectors for Antiviral Therapy ....... 313 ScotD. Henry, Quiwei Pan, andLucJ.W. van der Laan 20 Intranasal Delivery of Antiviral siRNA ................................. 333 Sailen Barik 21 Antibody-Mediated Delivery of siRNAs for Anti-HW Therapy ............... 339 Sanß-Soo Kim, Sandesh Subramanya, Dan Peer, Motornu Shimaoka, and Premiata Shankar 22 Aptamer-Targeted RNAi for HIV-1 Therapy ............................ 355 Jiehua Zhou and John J. Rossi Part V RNAi Screens to Study Virus-Host Interactions 23 RNAi Screening for Host Factors Involved in Viral Infection Using Drosophila Cells ............................................. 375 Sara Cherry 24 Genome-Wide RNAi Screen for Viral Replication in Mammalian Cell Culture .......................................... 383 Bhupesh K. Prusty, Alexander Karlas, Thomas F. Meyer, and Thomas Rudel 25 RNAi Screening in Mammalian Cells to Identify Novel Host Cell Molecules Involved in the Regulation of Viral Infections ................ 397 Carolyn B. Coyne and Sara Cherry Index .................................................................. 407
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spelling	Antiviral RNAi concepts, methods, and applications ed. by Ronald P. van Rij New York, NY [u.a.] Humana Press 2011 XI, 411 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 721 Springer protocols Rij, Ronald P. van Sonstige oth Erscheint auch als Online-Ausgabe 978-1-61779-037-9 Methods in molecular biology 721 (DE-604)BV035362695 721 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=021103710&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
spellingShingle	Antiviral RNAi concepts, methods, and applications Methods in molecular biology
title	Antiviral RNAi concepts, methods, and applications
title_auth	Antiviral RNAi concepts, methods, and applications
title_exact_search	Antiviral RNAi concepts, methods, and applications
title_full	Antiviral RNAi concepts, methods, and applications ed. by Ronald P. van Rij
title_fullStr	Antiviral RNAi concepts, methods, and applications ed. by Ronald P. van Rij
title_full_unstemmed	Antiviral RNAi concepts, methods, and applications ed. by Ronald P. van Rij
title_short	Antiviral RNAi
title_sort	antiviral rnai concepts methods and applications
title_sub	concepts, methods, and applications
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