Characterization of the UBX protein Ubx5 from Saccharomyces cerevisiae:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
München
Verl. Dr. Hut
2010
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| Ausgabe: | 1. Aufl. |
| Schriftenreihe: | Biochemie
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 109 S. Ill., graph. Darst. 210 mm x 148 mm, 163 g |
| ISBN: | 9783868537154 |
Internformat
MARC
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| 100 | 1 | |a Temiz, Silke |d 1980- |e Verfasser |0 (DE-588)143272926 |4 aut | |
| 245 | 1 | 0 | |a Characterization of the UBX protein Ubx5 from Saccharomyces cerevisiae |c Silke Temiz |
| 250 | |a 1. Aufl. | ||
| 264 | 1 | |a München |b Verl. Dr. Hut |c 2010 | |
| 300 | |a 109 S. |b Ill., graph. Darst. |c 210 mm x 148 mm, 163 g | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 0 | |a Biochemie | |
| 502 | |a Zugl.: München, Techn. Univ., Diss., 2010 | ||
| 650 | 0 | 7 | |a Kernproteine |0 (DE-588)4389511-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Saccharomyces cerevisiae |0 (DE-588)4178812-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Ubiquitin-Protein-Ligase |0 (DE-588)4398417-4 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Saccharomyces cerevisiae |0 (DE-588)4178812-6 |D s |
| 689 | 0 | 1 | |a Kernproteine |0 (DE-588)4389511-6 |D s |
| 689 | 0 | 2 | |a Ubiquitin-Protein-Ligase |0 (DE-588)4398417-4 |D s |
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Datensatz im Suchindex
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| adam_text | IMAGE 1
TABLE OF CONTENTS
1. SUMMARY 1
2. INTRODUCTION 5
2.1 THE UBIQUITIN/PROTEASOME SYSTEM (UPS) 5
2.1.1 THE UBIQUITIN CONJUGATION SYSTEM 5
2.1.2 FUNCTIONS OF THE UBIQUITIN SYSTEM IN S. CEREVISIAE. 8
2.1.3 UBIQUITIN BINDING DOMAINS ( U B D S) 11
2.1.4 THE 26S PROTEASOME 13
2.2 SUBSTRATE SPECIFICITY OF THE UBIQUITIN/PROTEASOME SYSTEM 15 2.2.1
CULLIN UBIQUITIN LIGASES 15
2.2.2 THE AAAATPASECDC48 21
2.2.3 COFACTORS OF CDC48 * 23
2.2.4 UBX DOMAIN PROTEINS 25
2.2.5 UBX5 29
2.3 AIM OF THIS WORK..»». »»..»»» 31
3 RESULTS 33
3.1 GENERAL CHARACTERIZATION OF UBX5........ 33
3.1.1 SUBCELLULAR LOCALIZATION OF UBX5 33
3.1.2 PHENOTYPES OF UBX5 35
3.1.3 GENETIC INTERACTIONS OF UBX5 WITH THE COFACTORS OF C D C 48 36
3.1.4 PHYSICAL INTERACTIONS OF UBX5 WITH OTHER COFACTORS OF C D C 48 38
3.1.5 INTERACTION OF THE UBX5 DOMAINS WITH CDC48 UF /NPI4 39
3 .2 U B X2 AND U B X5 INTERACT WITH CULLINS 45
3 . 2 .1 UBX5 INTERACTS WITH CULLINS 45
3.2.2 UBX5 RECRUITS CDC48 TO ROCL AND CDC53 47
3.2.3 UBX5 RECRUITS CDC48 TO MMS22 48
3.2.4 GENETIC INTERACTIONS OF UBX5 52
3.2.5 UBX2 INTERACTS WITH CULLINS 53
3.2.6 UBX2 AND UBX5 INTERACT WITH RTT107 AND TOPL 56
3.2.7 UBX2 IS INVOLVED IN NUCLEAR PROCESSES - 58
4. DISCUSSION 61
4.1 INTERACTION OF UBX5 WITH OTHER CDC48 COFACTORS 62
4.2 UBX5 RECRUITS CDC48 TO CDC53 64
4 .3 UBXS AND UBX2 ARE IMPLICATED IN D NA DAMAGE REPAIR 66
4 .4 UBX5 AND UBX2 ARE IMPLICATED IN THE PROCESSING OF STALLED
REPLICATION FORKS 68 4.5 THE RELATIONSHIP BETWEEN UBX2 AND UBX5 70
5. MATERIAL A ND M E T H O DS 73
5 .1 MATERIALS ........... .........»....».»....»»»».»»..»».... .. ...»»
»..»»»»»»»....».»...... 73
5.1.1 CHEMICALS AND REAGENTS 73
5.1.2 ANTIBODIES - 73
5.1.3 COMPUTATIONAL ANALYSES 74
5.1.4 . COT; STRAINS 74
5.1.5 E.COLI VECTORS - 74
5.1.6 E.COLI MEDIA. ~ - 74
5.1.75. CEREVISIAE STRAINS 74
5.1.85. CEREVISIAE VECTORS . - 75
5.1.95. CEREVISIAE PRIMERS * - 75
BIBLIOGRAFISCHE INFORMATIONEN HTTP://D-NB.INFO/1009230514
DIGITALISIERT DURCH
IMAGE 2
5 . 1 . 1 0 5. CEREVISIAE PLASMIDS 76
5 . 1 . 1 1 5. CEREVISIAE MEDIA AND SOLUTIONS 76
5.2 E.COLI TECHNIQUES 77
5.2.1 CULTIVATION AND STORAGE OF E.COLI. 77
5.2.2 PREPARATION OF COMPETENT COU CELLS 78
5.2.3 TRANSFORMATION OF PLASMID DNA INTO E. COU 78
5.2.4 EXPRESSION OF RECOMBINANT PROTEINS IN E.COLI 79
5 . 3 5. CEREVISIAE TECHNIQUES 79
5.3.1 CULTIVATION OF 5. CEREVISIAE 79
5.3.2 PREPARATION OF COMPETENT 5. CEREVISIAE CELLS 79
5.3.3 TRANSFORMATION OF 5. CEREVISIAE CELLS 79
5.3.4 GENOMIC INTEGRATION BY HOMOLOGOUS RECOMBINATION 80
5.3.5 MATING TYPE ANALYSIS OF HAPLOID MUTANTS 80
5.3.6 MATING OF HAPLOID 5. CEREVISIAE STRAINS 81
5 . 3 .7 SPORULATION AND TETRADE ANALYSIS OF DIPLOID 5. CEREVISIAE
STRAINS 81
5.3.8 PHENOTYPE ANALYSIS BY GROWTH TESTS 81
5.3.9 ANALYSES OF PROTEIN-PROTEIN INTERACTIONS USING THE TWO-HYBRID
SYSTEM 82 5.3.10 LIVE-CELL MICROSCOPY 82
5.4 PROTEIN BIOCHEMICAL METHODS.. 82
5.4.1 GEL ELECTROPHORESIS AND IMMUNOBLOTTING TECHNIQUES 82
5.4.2 PREPARATION OF CELL EXTRACTS 85
5.4.3 PROTEIN PURIFICATION AND BINDING STUDIES 85
5.5 MOLECULAR BIOLOGY METHODS 90
5.5.1 ISOLATION OF DNA 90
5.5.2 POLYMERASE CHAIN REACTION (PCR) 91
5.5.3 MOLECULAR CLONING METHODS 95
6. REFERENCES 97
|
| any_adam_object | 1 |
| author | Temiz, Silke 1980- |
| author_GND | (DE-588)143272926 |
| author_facet | Temiz, Silke 1980- |
| author_role | aut |
| author_sort | Temiz, Silke 1980- |
| author_variant | s t st |
| building | Verbundindex |
| bvnumber | BV036962097 |
| classification_tum | CHE 820d |
| ctrlnum | (OCoLC)706972860 (DE-599)DNB1009230514 |
| dewey-full | 572.84 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.84 |
| dewey-search | 572.84 |
| dewey-sort | 3572.84 |
| dewey-tens | 570 - Biology |
| discipline | Biologie Chemie |
| edition | 1. Aufl. |
| format | Thesis Book |
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| id | DE-604.BV036962097 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T22:51:37Z |
| institution | BVB |
| isbn | 9783868537154 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-020876968 |
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| physical | 109 S. Ill., graph. Darst. 210 mm x 148 mm, 163 g |
| publishDate | 2010 |
| publishDateSearch | 2010 |
| publishDateSort | 2010 |
| publisher | Verl. Dr. Hut |
| record_format | marc |
| series2 | Biochemie |
| spelling | Temiz, Silke 1980- Verfasser (DE-588)143272926 aut Characterization of the UBX protein Ubx5 from Saccharomyces cerevisiae Silke Temiz 1. Aufl. München Verl. Dr. Hut 2010 109 S. Ill., graph. Darst. 210 mm x 148 mm, 163 g txt rdacontent n rdamedia nc rdacarrier Biochemie Zugl.: München, Techn. Univ., Diss., 2010 Kernproteine (DE-588)4389511-6 gnd rswk-swf Saccharomyces cerevisiae (DE-588)4178812-6 gnd rswk-swf Ubiquitin-Protein-Ligase (DE-588)4398417-4 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Saccharomyces cerevisiae (DE-588)4178812-6 s Kernproteine (DE-588)4389511-6 s Ubiquitin-Protein-Ligase (DE-588)4398417-4 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020876968&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Temiz, Silke 1980- Characterization of the UBX protein Ubx5 from Saccharomyces cerevisiae Kernproteine (DE-588)4389511-6 gnd Saccharomyces cerevisiae (DE-588)4178812-6 gnd Ubiquitin-Protein-Ligase (DE-588)4398417-4 gnd |
| subject_GND | (DE-588)4389511-6 (DE-588)4178812-6 (DE-588)4398417-4 (DE-588)4113937-9 |
| title | Characterization of the UBX protein Ubx5 from Saccharomyces cerevisiae |
| title_auth | Characterization of the UBX protein Ubx5 from Saccharomyces cerevisiae |
| title_exact_search | Characterization of the UBX protein Ubx5 from Saccharomyces cerevisiae |
| title_full | Characterization of the UBX protein Ubx5 from Saccharomyces cerevisiae Silke Temiz |
| title_fullStr | Characterization of the UBX protein Ubx5 from Saccharomyces cerevisiae Silke Temiz |
| title_full_unstemmed | Characterization of the UBX protein Ubx5 from Saccharomyces cerevisiae Silke Temiz |
| title_short | Characterization of the UBX protein Ubx5 from Saccharomyces cerevisiae |
| title_sort | characterization of the ubx protein ubx5 from saccharomyces cerevisiae |
| topic | Kernproteine (DE-588)4389511-6 gnd Saccharomyces cerevisiae (DE-588)4178812-6 gnd Ubiquitin-Protein-Ligase (DE-588)4398417-4 gnd |
| topic_facet | Kernproteine Saccharomyces cerevisiae Ubiquitin-Protein-Ligase Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020876968&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT temizsilke characterizationoftheubxproteinubx5fromsaccharomycescerevisiae |