FLIM microscopy in biology and medicine:
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Boca Raton, Fla. [u.a.]
CRC Press
2010
|
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XXIX, 407 S., [16] Bl. Ill., graph. Darst. |
| ISBN: | 9781420078909 1420078909 |
Internformat
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV036953355 | ||
| 003 | DE-604 | ||
| 005 | 20200803 | ||
| 007 | t | ||
| 008 | 110112s2010 ad|| |||| 00||| eng d | ||
| 020 | |a 9781420078909 |9 978-1-4200-7890-9 | ||
| 020 | |a 1420078909 |9 1-4200-7890-9 | ||
| 035 | |a (OCoLC)699094315 | ||
| 035 | |a (DE-599)HBZHT015985210 | ||
| 040 | |a DE-604 |b ger |e rakwb | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-91 |a DE-11 | ||
| 082 | 0 | |a 570.282 | |
| 084 | |a WC 3100 |0 (DE-625)148081: |2 rvk | ||
| 084 | |a MED 371f |2 stub | ||
| 084 | |a FEI 320f |2 stub | ||
| 245 | 1 | 0 | |a FLIM microscopy in biology and medicine |c ed. by Ammasi Periasamy ... |
| 264 | 1 | |a Boca Raton, Fla. [u.a.] |b CRC Press |c 2010 | |
| 300 | |a XXIX, 407 S., [16] Bl. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 700 | 1 | |a Periasamy, Ammasi |4 edt | |
| 856 | 4 | 2 | |m HEBIS Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020868368&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-020868368 | ||
Datensatz im Suchindex
| _version_ | 1804143674827735040 |
|---|---|
| adam_text | IMAGE 1
FLIM MICROSCOPY
IN
BIOLOGY AND MEDICINE
EDITED BY
AMMASI PERIASAMY
ROBERT M. CLEGG
LTFI) CRC PRESS V^F J TAYLOR & FRANCIS CROUP BOCA RATON LONDON NEW YORK
CRC PRESS IS AN IMPRINT OF THE TAYLOR & FRANCIS CROUP AN INFORMA
BUSINESS
A CHAPMAN & HALL BOOK
IMAGE 2
TABLE OF CONTENTS
PREFACE, XIX ACKNOWLEDGMENTS, XXIII
THE EDITORS, XXV
CONTRIBUTORS, XXVII
SECTION 1 INTRODUCTION, MICROSCOPY, FLUOROPHORES
CHAPTER 1 * FLUORESCENCE LIFETIME-RESOLVED IMAGING:
WHAT, WHY, HOW-A PROLOGUE 3
ROBERT M. CLECC
1.1 INTRODUCTION . 3
1.2 GOAL OF THIS CHAPTER 4
1.3 WHY MEASURE FLUORESCENCE LIFETIMES? 4
1.4 WHY MEASURE LIFETIME-RESOLVED IMAGES? 7
1.5 SPECIFIC FEATURES OF THE DIFFERENT PATHWAYS AND
RATES OF DE-EXCITATION 8
1.5.1 INTRINSIC RATE OF EMISSION (FLUORESCENCE) 8
1.5.2 THERMAL RELAXATION (INTERNAL CONVERSION) 10
1.5.3 MOLECULAR RELAXATION OF THE SOLVENT OR MOLECULAR
MATRIX ENVIRONMENT 11
1.5.4 QUENCHERS (DYNAMIC) 12
1.5.5 EXCITED-STATE REACTIONS 12
1.5.6 FORSTER RESONANCE ENERGY TRANSFER (FRET) 13
1.5.7 INTERSYSTEM CROSSING AND DELAYED EMISSION 15
1.5.8 SLOW LUMINESCENCE WITHOUT INTERSYSTEM CROSSING 16
1.5.9 PHOTOLYSIS (PROCESS AND INTERPRETATION OF ITS MEASUREMENT) 17
1.5.10 THE UNIFYING FEATURE OF EXTRACTING INFORMATION FROM EXCITED-STATE
PATHWAYS 18
IMAGE 3
VIII * TABLE OF CONTENTS
1.6 OTHER PARAMETERS RELATED TO LIFETIME-RESOLVED FLUORESCENCE-DYNAMIC
AND STEADY-STATE MEASUREMENTS 18
1.6.1 ANISOTROPY DECAY ^^ 18
1.6.2 STEADY-STATE QUENCHING (DYNAMIC) MEASUREMENT ^^ 21
1.7 DATA ACQUISITION /^ 21
1.7.1 SCANNING AND FULL FIELD 21
1.7.1.1 SCANNING MODES 22
1.7.1.2 FULL-FIELD MODES 23
1.7.2 TIME AND FREQUENCY DOMAINS 23
1.7.2.1 TIME DOMAIN 24
1.7.2.2 FREQUENCY DOMAIN 25
1.7.3 EQUIVALENCE OF TIME AND FREQUENCY DOMAINS 26
1.7.4 PERFORMANCE GOALS AND COMPARISONS 26
1.8 DATA ANALYSIS 27
1.9 DISPLAY OF LIFETIME-RESOLVED IMAGES 28
1.10 SUMMARY , 28
REFERENCES 29
CHAPTER 2 * PRINCIPLES OF FLUORESCENCE FOR QUANTITATIVE FLUORESCENCE
MICROSCOPY 35
NEIL ANTHONY, PENG GUO, AND KEITH BERLAND
2.1 INTRODUCTION 35
2.2 WHAT IS FLUORESCENCE? 35
2.3 ABSORPTION 36
2.3.1 MOLECULAR EXCITATION RATES 38
2.3.1.1 ONE-PHOTON EXCITATION 39
2.3.1.2 TWO-PHOTON EXCITATION 41
2.4 FLUORESCENCE AND MOLECULAR RELAXATION PATHWAYS 45
2.4.1 INTERNAL CONVERSION 45
2.4.2 FLUORESCENCE EMISSION 45
2.4.2.1 QUANTUM YIELD 47
2.4.2.2 FLUORESCENCE LIFETIMES 47
2.4.2.3 FLUORESCENCE EMISSION SPECTRA 49
IMAGE 4
TABLE OF CONTENTS * IX
2.4.3 NONRADIATIVE RELAXATION PATHWAYS 50
2.4.3.1 BASICS OF FRET 51
2.4.3.2 INTERSYSTEM CROSSING AND PHOSPHORESCENCE 52
2.4.4 PHOTOSELECTION AND ANISOTROPY ^ ^- ~~~~ 52
2.5 MEASURING FLUORESCENCE IN THE MICROSCOPE 54
2.5.1 SENSITIVITY OF FLUORESCENCE MEASUREMENTS 54
2.5.1.1 FLUORESCENCE SIGNALS 56
2.5.2 OBSERVATION VOLUMES AND MOLECULAR BRIGHTNESS 56
2.5.3 SATURATION 57
2.5.4 PHOTOBLEACHING 58
2.6 SUMMARY 59
REFERENCES 59
CHAPTER 3 -VISIBLE FLUORESCENT PROTEINS FOR FRET-FLIM 65
RICHARD N. DAY
3.1 INTRODUCTION 65
3.2 BACKGROUND . 66
3.2.1 OVERVIEW OF THE FLUORESCENT PROTEINS 66
3.2.2 SPECTRAL VARIANTS FROM THE AEQUOREA GFP 68
3.2.3 AEQUOREA FLUORESCENT PROTEINS AND DIMER FORMATION 70
3.2.4 NEW FLUORESCENT PROTEINS FROM CORALS 70
3.3 METHODS 72
3.3.1 VISIBLE FLUORESCENT PROTEINS FOR FRET MEASUREMENTS 72
3.3.2 STANDARDS FOR LIVE-CELL FRET IMAGING 73
3.3.3 USING FRET-FLIM TO DETECT PROTEIN INTERACTIONS IN LIVING CELLS 75
3.3.4 VERIFYING PROTEIN INTERACTIONS USING ACCEPTOR
PHOTOBLEACHING FRET 77
3.3.5 ALTERNATIVE FLUOROPHORE PAIRS FOR FRET-FLIM 77
3.3.6 FLUORESCENT PROTEINS DESIGNED SPECIFICALLY FOR FLIM APPLICATIONS
79
3.4 CRITICAL DISCUSSION 81
3.4.1 GENERAL CONSIDERATIONS AND LIMITATIONS 81
3.4.2 OVEREXPRESSION ARTIFACTS 82
3.4.3 FACTORS LIMITING FRET-FLIM 82
3.4.4 FALSE POSITIVES AND FALSE NEGATIVES 83
3.4.5 ANALYSIS IN THE CELL POPULATION 83
IMAGE 5
* TABLE OF CONTENTS
3.5 SUMMARY 84
3.6 FUTURE PERSPECTIVE 84
REFERENCES 84
SECTION 2 INSTRUMENTATION
CHAPTER 4 -WIDE-FIELD FLUORESCENCE LIFETIME IMAGING MICROSCOPY USING A
GATED IMAGE INTENSIFIER CAMERA 93
YUANSHENC SUN, JAMES N. DEMAS, AND AMMASI PERIASAMY
4.1 INTRODUCTION 93
4.2 BACKGROUND 95
4.3 METHODS 95
4.3.1 THEORY BEHIND THE RLD METHOD 95
4.3.1.1 SINGLE-EXPONENTIAL DECAY 96
4.3.1.2 DOUBLE-EXPONENTIAL DECAY 97
4.3.2 COMPONENTS REQUIRED FOR RLD-BASED LIFETIME IMAGING 99
4.3.3 HOW DATA WERE ACQUIRED USING THE RLD METHOD 100
4.3.3.1 CALIBRATION OF THE SYSTEM WITH A KNOWN FLUOROPHORE
(SINGLE-EXPONENTIAL DECAY) 100
4.3.3.2 DOUBLE-EXPONENTIAL DECAYS: BIOLOGICAL EXAMPLES 102
4.4 CRITICAL DISCUSSION 106
4.5 PITFALLS 107
4.6 SUMMARY 110
APPENDIX 4.1: MONTE CARLO SIMULATION 110
APPENDIX 4.2: PREPARATION OF CELLS 111
REFERENCES 112
CHAPTER 5 * FREQUENCY-DOMAIN FLIM 115
BRYAN Q. SPRING AND ROBERT M. CLEGG
5.1 INTRODUCTION TO FREQUENCY-DOMAIN METHODS 115
5.1.1 OVERVIEW 115
5.1.2 HETERODYNE AND HOMODYNE METHODS FOR MEASURING FLUORESCENCE
LIFETIMES 116
5.1.3 A FEW PRELIMINARY COMMENTS 117
IMAGE 6
TABLE OF CONTENTS * XI
5.2 RELATIONSHIP BETWEEN OBSERVABLES AND
FLUORESCENCE LIFETIMES 117
5.2.1 A PRIMER IN COMPLEX ANALYSIS 117
5.2.2 A GENERAL EXPRESSION FOR THE FLUORESCENCE SIGNAL 117
5.2.3 A GENERAL EXPRESSION FOR THE MEASURED
HOMO-/HETERODYNE SIGNAL 121
5.2.4 SINGLE- VERSUS MULTIFFEQUENCY FLIM 122
5.2.4.1 SINGLE-FREQUENCY FLIM 122
5.2.4.2 MULTIFREQUENCY FLIM 123
5.2.4.3 HOMODYNE MULTIFREQUENCY FLIM 124
5.2.4.4 HETERODYNE MULTIFREQUENCY FLIM 125
5.3 EXTRACTING THE DEMODULATION AND PHASE SHIFT VALUES USING A DIGITAL
FOURIER TRANSFORM 126
5.4 VIDEO-RATE FLIM 127
5.4.1 OVERVIEW 127
5.4.2 INSTRUMENTATION 127
5.4.2.1 ILLUMINATION SOURCES AND ELECTRO-OPTICS FOR MODULATED EXCITATION
LIGHT 127
5.4.2.2 GAIN-MODULATED IMAGE INTENSIFIES 129
5.4.2.3 OPTICAL SETUP AND ELECTRONICS 130
5.4.3 CORRECTIONS FOR RANDOM NOISE AND SYSTEMATIC ERRORS 131
5.4.3.1 CORRECTING FOR LASER FLUCTUATIONS AND DARK CURRENT 131
5.4.3.2 GAIN-MODULATED IMAGE INTENSIFIER PERFORMANCE 131
5.5 ENHANCED FLIM MODES 133
5.5.1 VIDEO-RATE CONFOCAL FLIM 133
5.5.2 RAPID SPECTRAL FLIM 134
5.6 DATA DISPLAY 136
5.6.1 DUAL-LAYER FLIM IMAGES 136
5.6.2 DUAL-LAYER FRACTIONAL CONCENTRATION IMAGES 137
5.7 SUMMARY 139
REFERENCES 139
CHAPTER 6 * LASER SCANNING CONFOCAL FLIM MICROSCOPY 143
HANS C. CERRITSEN, ARIEN BADER, AND SASHA AGRONSKAIA
6.1 INTRODUCTION 143
6.1.1 HISTORICAL BACKGROUND 145
IMAGE 7
XII * TABLE OF CONTENTS
6.2 LIFETIME DETECTION METHODS IN SCANNING MICROSCOPY 146
6.2.1 TIME-CORRELATED SINGLE-PHOTON COUNTING (TCSPC) 146
6.2.2 TIME GATING / 149
6.3 DETECTORS AND ELECTRONICS 151
6.3.1 DETECTORS / 151
6.3.2 FRONT-END ELECTRONICS 153
6.4 COUNT RATE AND^ACQUISITION TIME 154
6.4.1 DETECTOR AND ELECTRONICS LIMITATIONS 155
6.4.2 EFFICIENCY OF TIME-DOMAIN LIFETIME DETECTION METHODS 156
6.5 EXAMPLE 158
6.6 SUMMARY 160
6.7 FUTURE PERSPECTIVE 160
REFERENCES 161
CHAPTER 7 * MULTIPHOTON FLUORESCENCE LIFETIME IMAGING
AT THE DAWN OF CLINICAL APPLICATION 165
KARSTEN KONIG AND AISADA UCHUGONOVA
7.1 INTRODUCTION * 165
7.2 PRINCIPLE OF MULTIPHOTON IMAGING 167
7.3 CLINICAL MULTIPHOTON TOMOGRAPHY 169
7.4 MULTIPHOTON FLIM TECHNIQUE 170
7.5 APPLICATIONS 174
7.5.1 MULTIPHOTON SKIN IMAGING 174
7.5.2 TWO-PHOTON FLIM IMAGING OF STEM CELLS 177
7.6 CONCLUSION 183
ACKNOWLEDGMENT 184
REFERENCES 184
CHAPTER 8 * FLIM MICROSCOPY W I TH A STREAK CAMERA: MONITORING
METABOLIC PROCESSES IN LIVING CELLS AND TISSUES 189
V. KRISHNAN RAMANUJAN, JAVIER A. JO, RAVI RANJAN, AND BRIAN A. HERMAN
8.1 INTRODUCTION 189
8.2 STREAKFLIM: SYSTEM INTEGRATION 190
8.2.1 STEP-BY-STEP DEMONSTRATION OF STREAKFLIM SYSTEM APPLICATION 193
8.2.1.1 DATA ACQUISITION 193
8.2.1.2 DATA ANALYSIS 195
IMAGE 8
TABLE OF CONTENTS * XIII
8.3 CRITICAL DISCUSSION 195
8.4 FURTHER APPLICATIONS / 200
8.4.1 IMAGING CANCER CELLS IN THREE^DIMENSIONAL ARCHITECTURE 200
8.4.2 KINETIC IMAGING OF PH TRANSIENTS DURING GLUCOSE METABOLISM 203
8.4.3 FLIM-BASED ENZYME ACTIVITY ASSAYS IN VIVO 205
8.5 SUMMARY AND FUTURETERSPECTIVE 207
REFERENCES / 208
CHAPTER 9 * SPECTRALLY RESOLVED FLUORESCENCE LIFETIME IMAGING
MICROSCOPY: SLIM/MWFLIM 211
CHRISTOPH BISKUP, BIRGIT HOFFMANN, KLAUS BENNDORF, AND ANGELIKA RUCK
9.1 INTRODUCTION 211
9.2 BACKGROUND 214
9.2.1 THE SPECTRAL AXIS OF THE FLUORESCENCE DECAY SURFACE 214
9.2.2 THE TIME AXIS OF THE FLUORESCENCE DECAY SURFACE 216
9.2.3 GLOBAL ANALYSIS 217
9.2.4 A SPECIAL CASE: GLOBAL ANALYSIS OF FRET MEASUREMENTS 218
9.3 METHODS - 224
9.3.1 THE SETUP 224
9.3.2 OPERATION PRINCIPLE OF THE STREAK CAMERA 226
9.3.3 OPERATION PRINCIPLE OF THE MWFLIM/SLIM SETUP 226
9.3.4 BENEFITS OF THE TECHNIQUES 228
9.3.5 CALIBRATION 229
9.3.5.1 CALIBRATION OF THE SPECTRAL AXIS 229
9.3.5.2 CALIBRATION OF THE TIME AXIS 230
9.3.5.3 CALIBRATION OF THE INTENSITY AXIS 230
9.3.6 DATA ANALYSIS 231
9.3.6.1 THE INSTRUMENT RESPONSE FUNCTION 231
9.3.6.2 DECONVOLUTION AND DATA FITTING 232
9.3.7 APPLICATIONS 234
9.3.7.1 FUNCTIONAL STAINING OF CELL STRUCTURES 234
9.3.7.2 PHOTODYNAMIC THERAPY (PDT) 234
9.3.7.3 FORSTER RESONANCE ENERGY TRANSFER 237
9.4 CRITICAL DISCUSSION 239
9.5 SUMMARY 241
REFERENCES 241
IMAGE 9
XIV * TABLE OF CONTENTS
CHAPTER 10 TIME-RESOLVED FLUORESCENCE ANISOTROPY 245
STEVEN S. VOGEL, CHRISTOPHER THALER, PAUL S^ BLANK, AND SRINAGESHV.
KOUSHIK
10.1 INTRODUCTION /^ 245
10.2 UNDERLYING CONCEPTS 246
10.3 LIGHT HAS AN ORIENTATION 247
10.4 PHOTOSELECTION 248
10.4.1 PHOTOSELECTION OF A RANDOMLY ORIENTED POPULATION
OF FLUOROPHORES 251
10.5 HOW DO WE DETECT POLARIZED EMISSIONS? 255
10.6 HOW DO WE QUANTIFY POLARIZED EMISSIONS? 261
10.7 THE ANISOTROPY OF RANDOMLY ORIENTED POPULATIONS
OF FLUOROPHORES 264
10.8 DEPOLARIZATION FACTORS AND SOLEILLET S RULE 265
10.8.1 INSTRUMENTAL DEPOLARIZATION 268
10.8.2 DEPOLARIZATION CAUSED BY ABSORPTION AND EMISSION
DIPOLE ORIENTATION 270
10.8.3 TIMESCALE OF DEPOLARIZATION 270
10.8.4 DEPOLARIZATION CAUSED BY MOLECULAR ROTATION 271
10.8.5 DEPOLARIZATION CAUSED BY FRET 275
10.9 FLUORESCENCE ANISOTROPY APPLICATIONS 282
10.9.1 PHOSPHORYLATION ASSAY 283
10.9.2 PUTTING LIMITS ON THE VALUE OF K 2 284
10.9.3 DIFFERENTIATING BETWEEN DIRECTLY EXCITED ACCEPTORS AND FRET 285
10.10 CONCLUSION 285
ACKNOWLEDGMENTS 286
REFERENCES 286
SECTION 3 DATA ANALYSIS
CHAPTER 11 * GENERAL CONCERNS OF FLIM DATA REPRESENTATION AND ANALYSIS:
FREQUENCY-DOMAIN MODEL-FREE ANALYSIS 291 YI-CHUN CHEN, BRYAN Q. SPRING,
CHITTANON BURANACHAI, BIANCATONG, GEORGE MALACHOWSKI, AND ROBERT M.
CLEGG
11.1 INTRODUCTION 291
11.2 TIME DOMAIN ASSUMING VERY SHORT EXCITATION PULSES 293
IMAGE 10
TABLE OF CONTENTS * XV
11.3 FREQUENCY DOMAIN 296
11.3.1 CALCULATING F(T) MEAS DIRECTLY FROM THE-CONVOLUTION INTEGRAL 296
11.3.2 CALCULATING F(T) MEAS FROM THE FINITE FOURIER TRANSFORM OF THE
REPETITIVE 5-PULSE RESULT / 299
11.3.3 CALCULATING THE FREQUENCY RESPONSE FROM THE CONVOLUTION THEOREM
OF FOURIERTRANSFORMS 301
11.4 ANALYSIS OF THE MEASURED DATA, F{T) MEAS , AT EVERY PIXEL 302
11.5 REMARKS ABOUT SIGNAL-TO-NOISE CHARACTERISTICS OF TIME- AND
FREQUENCY-DOMAIN SIGNALS: COMPARISON TO SINGLE- CHANNEL EXPERIMENTS 303
11.6 FLIM EXPERIMENTS: CHALLENGES, ADVANTAGES,
AND SOLUTIONS 305
11.7 HOW FLIM CIRCUMVENTS THE DATA DELUGE 306
11.7.1 POLAR PLOTS OF FREQUENCY-DOMAIN DATA (MODEL-FREE ANALYSIS) 307
11.7.1.1 POLAR PLOT DESCRIPTION OF FLUORESCENCE DIRECTLY EXCITED BY
LIGHT PULSES 307
11.7.1.2 POLAR PLOT OF FLUORESCENCE FROM A PRODUCT SPECIES OF AN
EXCITED-STATE REACTION 311
11.7.2 COMBINING SPECTRA AND POLAR PLOTS 314
11.7.2.1 TWO DIFFERENT NONINTERACTINGFLUOROPHORES 315
11.7.2.2 FRET: OBSERVING DONOR AND ACCEPTOR FLUORESCENCE SIMULTANEOUSLY
318
11.8 WAVELETS AND DENOISING 320
11.8.1 WHY USE THIS IMAGE ANALYSIS? 320
11.8.2 WAVELET TRANSFORMS FOR DISCRIMINATING FLUORESCENCE LIFETIMES
BASED ON SPATIAL MORPHOLOGY 321
11.8.2.1 WHAT IS A WAVELET TRANSFORM? 321
11.8.2.2 APPLICATIONS OF WAVELETS TO HOMODYNE FLIM 324
11.8.3 DENOISING HOMODYNE FLIM DATA 325
11.8.3.1 SOURCES OF NOISE FOR HOMODYNE FLIM 325
11.8.3.2 REMOVAL OF SIGNAL-DEPENDENT NOISE: TI-HAAR DENOISING 325
11.8.3.3 TI-HAAR DENOISING IMPROVES HOMODYNE FLIM ACCURACY 326 11.8.4
THE FUTURE OF WAVELET AND DENOISING IMAGE ANALYSIS FOR HOMODYNE FLIM 328
11.9 NONITERATIVE DATA REGRESSION (CHEBYSHEV AND LAGUERRE POLYNOMIALS)
330
11.9.1 NONITERATIVE DATA REGRESSION 330
IMAGE 11
XVI * TABLE OF CONTENTS
11.9.2 CONVEXITY IN MODELING AND MULTIPLE SOLUTIONS 330
11.9.2.1 FORMULATION OF MODELING AS A DYNAMIC SYSTEM 332
11.9.2.2 SOLUTION TO CONVEXITY IN A HILBERT SPACE 332
11.9.2.3 ERROR EVALUATION 334
REFERENCES / 335
CHAPTER 12 * NONLINEAR CURVE-FITTING METHODS FOR TIME-RESOLVED DATA
ANALYSIS 341
IGNACY CRYCZYNSKI, RAFAL LUCHOWSKI, SHASHANK BHARILL, JULIAN BOREJDO,
AND ZYGMUNT GRYCZYNSKI
12.1 INTRODUCTION 341
12.2 BACKGROUND 342
12.3 METHODS 343
12.3.1 BASIC TERMINOLOGY AND ASSUMPTIONS 343
12.3.2 LEAST-SQUARES ANALYSIS 345
12.3.2.1 TIME DOMAIN 346
12.3.2.2 FREQUENCY DOMAIN 348
12.3.3 LEAST-SQUARES PARAMETER ESTIMATION 349
12.3.4 DIAGNOSTICS FBR QUALITY OF CURVE-FITTING RESULTS 350
12.3.5 UNCERTAINTY OF CURVE-FITTING PROCEDURES 350
12.4 EXAMPLES 351
12.4.1 HOW TO ANALYZE EXPERIMENTAL DATA 351
12.4.2 SYSTEMATIC ERRORS 352
12.4.2.1 LIGHT DELAY 352
12.4.2.2 COLOR EFFECT IN THE DETECTOR 353
12.4.2.3 POLARIZATION EFFECT 355
12.4.2.4 PILEUP EFFECT 356
12.4.2.5 SOLVENT EFFECT 357
12.4.3 ANALYSIS OF MULTIEXPONENTIAL DECAYS 358
12.4.3.1 EFFECT OF THE SIGNAL LEVEL 359
12.4.3.2 TWO AND THREE COMPONENTS OF INTENSITY DECAYS 361
12.4.3.3 FLUORESCENCE LIFETIME DISTRIBUTION: BIOLOGICAL EXAMPLES 364
12.5 SUMMARY 367
REFERENCES . 368
IMAGE 12
TABLE OF CONTENTS * XVII
CHAPTER 13 * GLOBAL ANALYSIS OF FREQUENCY DOJNAIN FLIM DATA 371
HERNAN E. GRECCO AND PETER J. VERVEER / /
13.1 INTRODUCTION / 371
13.2 FOURIER DESCRIPTION Q F F L IM DATA 372
13.3 GLOBAL ANALYSIS OF/FLIM DATA 374
13.4 APPLICATION TO FRET-FLIM DATA 375
13.5 DISCUSSION AND OUTLOOK 375
13.6 SUMMARY 380
APPENDIX 13.1: METHODS 380
CELL PREPARATION 380
FLUORESCENCE LIFETIME IMAGING MICROSCOPY 381
REFERENCES 381
SECTION 4 APPLICATIONS
CHAPTER 14 * FLIM APPLICATIONS IN THE BIOMEDICAL SCIENCES 385
AMMASI PERIASAMY AND ROBERT M. CLEGG
14.1 INTRODUCTION 385
14.2 A BRIEF HISTORICAL JOURNEY THROUGH THE DEVELOPMENT OF
LIFETIME-RESOLVED IMAGING 386
14.3. AUTOFLUORESCENCE LIFETIME IMAGING OF CELLS 388
14.4 PAP SMEAR DETECTION USING TIME-GATED LIFETIME
IMAGING MICROSCOPY 390
14.5 FLIM IN ALZHEIMER S DISEASE 394
14.6 OPTICAL PROJECTION OF FLIM IMAGES OF MOUSE EMBRYO 394
14.7 FULL-FIELD FLIM WITH QUADRANT DETECTOR 395
14.8 CONCLUSION 396
REFERENCES 398
INDEX, 401
|
| any_adam_object | 1 |
| author2 | Periasamy, Ammasi |
| author2_role | edt |
| author2_variant | a p ap |
| author_facet | Periasamy, Ammasi |
| building | Verbundindex |
| bvnumber | BV036953355 |
| classification_rvk | WC 3100 |
| classification_tum | MED 371f FEI 320f |
| ctrlnum | (OCoLC)699094315 (DE-599)HBZHT015985210 |
| dewey-full | 570.282 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 570 - Biology |
| dewey-raw | 570.282 |
| dewey-search | 570.282 |
| dewey-sort | 3570.282 |
| dewey-tens | 570 - Biology |
| discipline | Biologie Medizintechnik Feinwerktechnik Medizin |
| format | Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01176nam a2200325 c 4500</leader><controlfield tag="001">BV036953355</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20200803 </controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">110112s2010 ad|| |||| 00||| eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9781420078909</subfield><subfield code="9">978-1-4200-7890-9</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">1420078909</subfield><subfield code="9">1-4200-7890-9</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)699094315</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)HBZHT015985210</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-91</subfield><subfield code="a">DE-11</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">570.282</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 3100</subfield><subfield code="0">(DE-625)148081:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">MED 371f</subfield><subfield code="2">stub</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">FEI 320f</subfield><subfield code="2">stub</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">FLIM microscopy in biology and medicine</subfield><subfield code="c">ed. by Ammasi Periasamy ...</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Boca Raton, Fla. [u.a.]</subfield><subfield code="b">CRC Press</subfield><subfield code="c">2010</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">XXIX, 407 S., [16] Bl.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Periasamy, Ammasi</subfield><subfield code="4">edt</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">HEBIS Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020868368&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-020868368</subfield></datafield></record></collection> |
| id | DE-604.BV036953355 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T22:51:25Z |
| institution | BVB |
| isbn | 9781420078909 1420078909 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-020868368 |
| oclc_num | 699094315 |
| open_access_boolean | |
| owner | DE-91 DE-BY-TUM DE-11 |
| owner_facet | DE-91 DE-BY-TUM DE-11 |
| physical | XXIX, 407 S., [16] Bl. Ill., graph. Darst. |
| publishDate | 2010 |
| publishDateSearch | 2010 |
| publishDateSort | 2010 |
| publisher | CRC Press |
| record_format | marc |
| spelling | FLIM microscopy in biology and medicine ed. by Ammasi Periasamy ... Boca Raton, Fla. [u.a.] CRC Press 2010 XXIX, 407 S., [16] Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Periasamy, Ammasi edt HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020868368&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | FLIM microscopy in biology and medicine |
| title | FLIM microscopy in biology and medicine |
| title_auth | FLIM microscopy in biology and medicine |
| title_exact_search | FLIM microscopy in biology and medicine |
| title_full | FLIM microscopy in biology and medicine ed. by Ammasi Periasamy ... |
| title_fullStr | FLIM microscopy in biology and medicine ed. by Ammasi Periasamy ... |
| title_full_unstemmed | FLIM microscopy in biology and medicine ed. by Ammasi Periasamy ... |
| title_short | FLIM microscopy in biology and medicine |
| title_sort | flim microscopy in biology and medicine |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020868368&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT periasamyammasi flimmicroscopyinbiologyandmedicine |