Dissection of a type I interferon pathway that controls intracellular Legionella pneumophila infection:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Berlin
Mensch & Buch Verl.
2010
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | IX, 99 S. Ill., graph. Darst. |
| ISBN: | 9783866648654 |
Internformat
MARC
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| 245 | 1 | 0 | |a Dissection of a type I interferon pathway that controls intracellular Legionella pneumophila infection |c vorgelegt von Juliane Lippmann |
| 264 | 1 | |a Berlin |b Mensch & Buch Verl. |c 2010 | |
| 300 | |a IX, 99 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Zugl.: Berlin, Humboldt-Univ., Diss., 2010 | ||
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Datensatz im Suchindex
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| adam_text | IMAGE 1
TABLE OF CONTENTS
TABLE OF CONTENTS
ZUSAMMENFASSUNG /
SUMMARY... //
TABLE OF CONTENTS ///
ABBREVIATIONS V
1 INTRODUCTION 1
1.1 THE INNATE AND THE ADAPTIVE IMMUNE SYSTEM 1
1.1.1 THE INNATE IMMUNE SYSTEM RELIES ON PATTERN RECOGNITION RECEPTORS 2
1.1.1.1 TLRS 3
1.1.1.2 NLRS 4
1.1.1.3 RLRS 6
1.1.1.4 CYTOSOLIC DNA-SENSORS 7
1.1.1.4.1 ZBP1 8
1.1.1.4.2 POL ILL/RIG-I 10
1.1.2 THE EFFECTORS OF THE INNATE IMMUNE SYSTEM 10
1.1.2.1 IFNS 10
1.1.2.1.1 TRANSCRIPTIONAL CONTROL OF TYPE I IFNS 11
1.1.2.1.2 IFNS ACTIVATE THE JAK-STAT PATHWAY 12
1.1.2.1.3 TYPE IIFN INDUCTION BY PRRS 13
1.1.2.1.4 TYPE I IFN INDUCTION BY BACTERIA 14
1.1.2.1.5 SIGNIFICANCE OF TYPE I IFNS IN HOST RESPONSE TO BACTERIA 15
1.1.2.2 TYPE I AND TYPE II IFN-MEDIATED IMMUNITY VIA IFN-STIMULATED
GENES 16 1.1.2.2.1 IMMUNITY-RELATED GTPASES 17
1.2 LEGIONELLA PNEUMOPHILA 18
1.3 L PNEUMOPHILA AND INNATE IMMUNITY - THE ROLE OF TLRS, NAIP5/NLRC4
AND IFNY.20 1.4 AIM OF THIS STUDY 24
2 RESULTS 25
2.1 L PNEUMOPHILA-INFECTED CELLS PRODUCED IFNSS IN A PROCESS REQUIRING
THE BACTERIAL T4SS, BACTERIAL UPTAKE AND THE RECOGNITION OF BACTERIAL
DNA 25
2.2 IFNSS PRODUCTION IN L. PNEUMOPHILA-INFECTED BMMS IS DEPENDENT ON
STING AND IRF3, WHEREAS THE TLRS, NOD2, ZBP1 AND MAVS WERE NOT REQUIRED
27 2.2.1 IFNSS RESPONSES INDUCED BY L. PNEUMOPHILA INFECTION OR CYTOSOLIC
DNA DO NOT REQUIRE HZBP1 IN HUMAN CELLS 29
2.3 TYPE I IFNS ACTIVATE A CELL-AUTONOMOUS RESISTANCE MECHANISM AGAINST
L PNEUMOPHILA THAT IS INDEPENDENT OF NAIP5/NLRC4 AND IFNY 32 2.4 THE
TYPE I IFN-MEDIATED RESISTANCE PATHWAY IMPACTS NUMBERS OF L PNEUMOPHILA
IN REPLICATION VACUOLES INDEPENDENTLY OF ER RECRUITMENT OR LYSOSOMAL
FUSION 34 2.5 IFNA/SS-MEDIATED INTRACELLULAR RESISTANCE AGAINST L.
PNEUMOPHILA APPEARS TO BE
INDEPENDENT OF PYROPTOSIS, AUTOPHAGY, RNI, ROI, IRON DEPRIVATION AND
TRYPTOPHAN DEPLETION 36
2.6 POTENTIAL ROLE OF ANTIBACTERIALLY-ACTING ISGS IN TYPE I
IFN-DEPENDENT CELL-AUTONOMOUS RESISTANCE AGAINST L. PNEUMOPHILA 38
2.7 ROLE OF TYPE I IFNS IN L PNEUMOPHILA INFECTION IN VIVO 40
3 DISCUSSION 43
3.1 BACTERIAL FACTORS REQUIRED FOR TYPE I IFN PRODUCTION DURING L.
PNEUMOPHILA INFECTION IN MURINE CELLS 44
3.2 HOST CELL MOLECULES INVOLVED IN TYPE I IFN PRODUCTION BY L.
PNEUMOPHILA 46 3.3 INTERACTION OF TYPE I IFNS WITH NAIP5/NLRC4 AND IFNY
IN CELL-AUTONOMOUS DEFENSE DURING L PNEUMOPHILA INFECTION 49
3.4 MECHANISMS OF THE INTRACELLULAR DEFENSE MEDIATED BY TYPE I IFN
DURING L. PNEUMOPHILA INFECTION 51
3.5 ROLE OF TYPE I IFNS IN L PNEUMOPHILA INFECTION IN VIVO 54
3.6 ASPECTS OF TYPE I IFN-MEDIATED DEFENSE IN MICE AND HUMANS 56
3.7 CONCLUSIONS AND OUTLOOK 59
BIBLIOGRAFISCHE INFORMATIONEN HTTP://D-NB.INFO/1009137476
DIGITALISIERT DURCH
IMAGE 2
TABLE OF CONTENTS
4 MATERIALS AND METHODS 61
4.1 BACTERIAL CULTURES 61
4.1.1 L PNEUMOPHILA 61
4.2 MICE 61
4.3 CELL CULTURES 62
4.3.1 HUMAN CELL CULTURES 62
4.3.2 MURINE CELL CULTURES 62
4.3.3 ISOLATION, GENERATION AND IN VITRO CULTURE OF BMMS 63
4.4 MOLECULAR BIOLOGY METHODS 63
4.4.1 RNA INTERFERENCE 63
4.4.2 EXPRESSION PLASMIDS AND CDNA TRANSFECTION 64
4.4.3 DNA AND RNA PURIFICATION 64
4.4.3.1 DNA AND RNA PURIFICATION FROM L PNEUMOPHILA 64
4.4.3.2 CELLULAR MRNA PREPARATION AND ANALYSIS 65
4.4.4 RT-PCR ANALYSIS 65
4.4.5 QUANTITATIVE RT-PCR 66
4.4.6 IMMUNOBLOTTING 66
4.4.6.1 TOTAL PROTEIN EXTRACTION 66
4.4.6.2 SDS POLYACRYLAMIDE GEL ELECTROPHORESIS (SDS-PAGE) 67 4.4.6.3
WESTERN BLOT AND DETECTION 67
4.4.6.4 BUFFERS AND GELS USED FOR IMMUNOBLOTTING 67
4.4.7 ELISA 68
4.5 CELLULAR BIOLOGY METHODS 68
4.5.1 CHEMICAL TREATMENTS 68
4.5.2 TRANSFECTIONS 68
4.5.3 BACTERIAL PREPARATIONS 68
4.5.4 INFECTIONS 69
4.5.5 BACTERIAL REPLICATION ASSAYS 69
4.5.5.1 IN VITRO INTRACELLULAR REPLICATION ASSAYS 69
4.5.5.2 IN VIVO MOUSE INFECTIONS AND BACTERIAL RECOVERY 70
4.5.5.3 IMMUNOFLUORESCENCE ASSAY TO MEASURE FORMATION OF VACUOLES
CONTAINING REPLICATING BACTERIA 70
4.5.6 LCV ISOLATION ASSAY 70
4.5.7 LDH DETECTION ASSAY 71
4.5.8 APOPTOSIS DETECTION ASSAY 71
4.5.9 LUCIFERASE REPORTER ASSAY 71
4.6 REAGENTS AND INSTRUMENTATION 72
5 REFERENCES 75
6 APPENDIX 93
CURRICULUM VLTAE 93
PUBLICATIONS. . 95
ACKNOWLEDGEMENTS 97
SELBSTAENDIGKEITSERKLAERUNG 99
IV
|
| any_adam_object | 1 |
| author | Lippmann, Juliane 1980- |
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| dewey-ones | 616 - Diseases |
| dewey-raw | 616.241 |
| dewey-search | 616.241 |
| dewey-sort | 3616.241 |
| dewey-tens | 610 - Medicine and health |
| discipline | Biologie Medizin |
| format | Thesis Book |
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| isbn | 9783866648654 |
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| spelling | Lippmann, Juliane 1980- Verfasser (DE-588)14319349X aut Dissection of a type I interferon pathway that controls intracellular Legionella pneumophila infection vorgelegt von Juliane Lippmann Berlin Mensch & Buch Verl. 2010 IX, 99 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zugl.: Berlin, Humboldt-Univ., Diss., 2010 Legionelleninfektion (DE-588)4192355-8 gnd rswk-swf Interferon (DE-588)4130836-0 gnd rswk-swf Angeborene Immunität (DE-588)4739713-5 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Legionelleninfektion (DE-588)4192355-8 s Angeborene Immunität (DE-588)4739713-5 s Interferon (DE-588)4130836-0 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020806002&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Lippmann, Juliane 1980- Dissection of a type I interferon pathway that controls intracellular Legionella pneumophila infection Legionelleninfektion (DE-588)4192355-8 gnd Interferon (DE-588)4130836-0 gnd Angeborene Immunität (DE-588)4739713-5 gnd |
| subject_GND | (DE-588)4192355-8 (DE-588)4130836-0 (DE-588)4739713-5 (DE-588)4113937-9 |
| title | Dissection of a type I interferon pathway that controls intracellular Legionella pneumophila infection |
| title_auth | Dissection of a type I interferon pathway that controls intracellular Legionella pneumophila infection |
| title_exact_search | Dissection of a type I interferon pathway that controls intracellular Legionella pneumophila infection |
| title_full | Dissection of a type I interferon pathway that controls intracellular Legionella pneumophila infection vorgelegt von Juliane Lippmann |
| title_fullStr | Dissection of a type I interferon pathway that controls intracellular Legionella pneumophila infection vorgelegt von Juliane Lippmann |
| title_full_unstemmed | Dissection of a type I interferon pathway that controls intracellular Legionella pneumophila infection vorgelegt von Juliane Lippmann |
| title_short | Dissection of a type I interferon pathway that controls intracellular Legionella pneumophila infection |
| title_sort | dissection of a type i interferon pathway that controls intracellular legionella pneumophila infection |
| topic | Legionelleninfektion (DE-588)4192355-8 gnd Interferon (DE-588)4130836-0 gnd Angeborene Immunität (DE-588)4739713-5 gnd |
| topic_facet | Legionelleninfektion Interferon Angeborene Immunität Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020806002&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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