Cryo-EM: C Analyses, interpretation, and case studies
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Amsterdam [u.a.]
Elsevier, Acad. Press
2010
|
| Ausgabe: | 1. ed. |
| Schriftenreihe: | Methods in enzymology
483 |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XLV, 360 S. Ill., graph. Darst. |
| ISBN: | 9780123849939 |
Internformat
MARC
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| 008 | 101208s2010 ad|| |||| 00||| eng d | ||
| 020 | |a 9780123849939 |9 978-0-12-384993-9 | ||
| 035 | |a (OCoLC)698574937 | ||
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| 041 | 0 | |a eng | |
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| 245 | 1 | 0 | |a Cryo-EM |n C |p Analyses, interpretation, and case studies |c ed. by Grant J. Jensen |
| 250 | |a 1. ed. | ||
| 264 | 1 | |a Amsterdam [u.a.] |b Elsevier, Acad. Press |c 2010 | |
| 300 | |a XLV, 360 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in enzymology |v 483 | |
| 490 | 0 | |a Methods in enzymology |v ... | |
| 700 | 1 | |a Jensen, Grant J. |e Sonstige |4 oth | |
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| 830 | 0 | |a Methods in enzymology |v 483 |w (DE-604)BV000000938 |9 483 | |
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| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-020774239 | |
Datensatz im Suchindex
| _version_ | 1813525205127004160 |
|---|---|
| adam_text |
Contents
Contributors
xi
Preface
xv
Volumes in Series
xvii
1.
Analyses of Subnanometer Resolution Cryo-EM Density Maps
1
Matthew
L
Baker, Mariah R. Baker, Corey F. Hryc, and Frank DiMaio
1.
Introduction
2
2.
Features in a Subnanometer Resolution Density Map
3
3.
Tools: Analyzing a Subnanometer Resolution Density Map
4
4.
Protocol: From Density Map to Atomic Model
9
5.
Case Study: Mm-cpn
21
6.
Discussion
24
Acknowledgments
25
References
25
2.
Methods for Segmentation and Interpretation of Electron
Tomographie
Reconstructions
31
Niels
Volkmann
1.
Introduction
32
2.
Noise Reduction
35
3.
Segmentation
36
4.
Detection and Mapping of Macromolecular Assemblies
38
5.
Classification and Averaging
39
6.
Validation
40
Acknowledgments
41
References
42
3.
Integration of Cryo-EM with Atomic and Protein-Protein
Interaction Data
47
Friedrich Förster
and Elizabeth Villa
1.
Introduction
48
2.
The Problem of Placing Assembly Subunits into Cryo-EM Maps
49
3.
Structure Prediction of Subunits
50
Contents
VI
_-------------
4. Protein-Protein
Interaction Data
5Ą
5.
Model Building of a Complex Using Cryo-EM and Additional Data
58
6.
Refinement of Atomic Models Using High-Resolution Maps
64
7.
Conclusion and Outlook 68
References 69
4.
Unified Data Resource for Cryo-EM
73
Catherine L. Lawson
1.
Introduction
74
2.
EM Structural Data Archives
75
3.
Deposition and Content 77
4.
Access
79
5.
Examples of Use
82
6.
Future Prospects 85
7.
Guide to Deposition of EM Structural Data
85
Acknowledgments
87
References
87
5.
Electron Crystallography and Aquaporins
91
Andreas D.
Schenk,
Richard K.
Hite,
Andreas
Engel, Yoshinori
Fujiyoshi,
and Thomas
Walz
1.
Electron Crystallography
92
2.
Contributions of Electron Crystallography to the Structural Biology
of Aquaporins
93
3.
Contributions of Structural Studies on Aquaporins to Advances
in Electron Crystallography
99
Acknowledgments
113
References
113
6.
Cryoelectron Microscopy Applications in the Study
of Tubulin Structure, Microtubule Architecture, Dynamics
and Assemblies, and Interaction of Microtubules with Motors
121
Kenneth H. Downing and
Eva Nogales
1.
Introduction: The Role of Electron Microscopy in Tubulin Studies
122
2.
Tubulin Studies by Electron Crystallography
123
3.
Microtubule Structure
128
4.
Structure of Microtubule Assembly/Disassembly Intermediates
131
5.
Mechanism of Kinesin Movement Along Microtubule
133
6.
Drug Binding Studied by Diffraction and Modeling
136
7.
Tomography for Larger Structures
137
8.
Conclusion
139
Contents
vii
Acknowledgments
139
References
140
7.
Helical Crystallization of Two Example Membrane Proteins:
MsbA and the Ca2+-ATPase
143
John Paul Glaves, Lauren Fisher, Andrew Ward, and Howard S. Young
1.
Helical Crystallization of the Bacterial Integral Membrane
Protein, MsbA
144
2.
Helical Crystallization of the Sarcoplasmic Reticulum Ca2+-ATPase
147
3.
Discussion
151
4.
Conclusions
156
Acknowledgments
157
References
157
8.
Multiparticle Cryo-EM of Ribosomes
161
Justus Loerke, Jan Giesebrecht, and Christian M. T. Spahn
1.
Introduction
162
2.
Dealing with Heterogeneity:
3D
Sorting
163
3.
Subnanometer Multiparticle Cryo-EM of Ribosomes in Practice
165
4.
Interpretation
171
5.
Conclusions
173
Acknowledgments
174
References
174
9.
Single-Particle Electron Microscopy of Animal Fatty
Acid Synthase: Describing Macromolecular Rearrangements
that Enable Catalysis
179
Edward J. Brignole and Francisco
Asturias
1.
Electron Microscopy and the Next Frontier in Structural Biology
180
2.
The Catalytic Cycle of FAS Requires Conformational Changes
180
3.
Methods and Rationale Employed in the Conformational
Analysis of FAS
182
4.
EM and FAS: A Versatile Tool for a Flexible
Macromolecule
195
Acknowledgments
198
References
199
10.
Tomography of Actin Cytoskeletal Networks
203
Dorit
Hanein
1.
Introduction
204
2.
Testing the Lamella Hypothesis
205
v¡¡¡
Contents
3.
Challenges
206
Acknowledgments
212
References
212
11.
Visual Proteomics
215
Friedrich
Förster, Bong-Gyoon
Han, and Martin
Beck
1.
Introduction
216
2.
Data Acquisition
217
3.
Templates
221
4.
Template Matching
227
5.
Assessment of Performance
232
6.
The Spatial Proteome of
L
'mterrogans
237
7.
Outlook
241
Acknowledgments
241
References
242
12.
Cryoelectron Tomography of Eukaryotic Cells
245
Asaf Mader, Nadav
Elad,
and Ohad
Medalia
1.
Introduction
246
2.
Specimen Preparation
248
3.
Relying on Correlative Light and Electron Microscope for Cellular
Structural Study of Eukaryotic Cells
252
4.
Cryoelectron Tomography of Cytoskeleton-Driven Processes
254
5.
Cryotomography of Midbodies
256
6.
Structural Analysis of the Nuclear Pore Complex by Cryo-ET
257
7.
Concluding Remarks
260
Acknowledgments
260
References
260
13. 3D
Visualization of
HIV
Virions
by Cryoelectron Tomography
267
]un Liu, Elizabeth R. Wright, and
Hanspeter Winkler
1.
Introduction
268
2.
Cryoelectron Tomography
270
3. 3D
Visualization of Intact HIVVirion
278
4.
Conclusions and Perspective
286
Acknowledgments
286
References
286
Contents ix
14. Automation in
Single-Particle
Electron
Microscopy:
Connecting the Pieces
291
Dmitry Lyumkis,
Arne
Moeller,
Anchi
Cheng, Amber
Herold,
Eric
Hou,
Christopher Irving, Erica
L Jacovetty,
Pick-Wei
Lau, Anke
АЛ.
Mulder,
James Pulokas, Joel D. Quispe, Neil R.
Voss,
Clinton S. Potter,
and Bridget Carragher
1.
Introduction
292
2.
Specimen Preparation
295
3.
Autoloading and Robotic Screening
300
4.
Microscopy
304
5.
Image Processing
311
6.
Assessment and Integration
322
7.
The Future of Automation
327
Acknowledgments
330
References
330
Author Index
339
Subject Index
355 |
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| illustrated | Illustrated |
| indexdate | 2024-10-21T12:06:50Z |
| institution | BVB |
| isbn | 9780123849939 |
| language | English |
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| physical | XLV, 360 S. Ill., graph. Darst. |
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| publisher | Elsevier, Acad. Press |
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| series | Methods in enzymology |
| series2 | Methods in enzymology |
| spelling | Cryo-EM C Analyses, interpretation, and case studies ed. by Grant J. Jensen 1. ed. Amsterdam [u.a.] Elsevier, Acad. Press 2010 XLV, 360 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in enzymology 483 Methods in enzymology ... Jensen, Grant J. Sonstige oth (DE-604)BV036854506 3 Methods in enzymology 483 (DE-604)BV000000938 483 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020774239&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Cryo-EM Methods in enzymology |
| title | Cryo-EM |
| title_auth | Cryo-EM |
| title_exact_search | Cryo-EM |
| title_full | Cryo-EM C Analyses, interpretation, and case studies ed. by Grant J. Jensen |
| title_fullStr | Cryo-EM C Analyses, interpretation, and case studies ed. by Grant J. Jensen |
| title_full_unstemmed | Cryo-EM C Analyses, interpretation, and case studies ed. by Grant J. Jensen |
| title_short | Cryo-EM |
| title_sort | cryo em analyses interpretation and case studies |
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