Functional glycomics:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Amsterdam [u.a.]
Elsevier, Acad. Press
2010
|
| Ausgabe: | 1. ed. |
| Schriftenreihe: | Methods in enzymology
479 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XLVIII, 447 S. Ill., graph. Darst. |
| ISBN: | 9780123809971 |
Internformat
MARC
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| 245 | 1 | 0 | |a Functional glycomics |c ed. by Minoru Fukuda |
| 250 | |a 1. ed. | ||
| 264 | 1 | |a Amsterdam [u.a.] |b Elsevier, Acad. Press |c 2010 | |
| 300 | |a XLVIII, 447 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-020709733 | ||
Datensatz im Suchindex
| _version_ | 1804143482101563392 |
|---|---|
| adam_text | Contents
Contributors
xiii
Preface
xix
Volumes in Series
xxi
Section I. Stem Cell
1
1.
^1,4-Galactosyltransferase V: A Growth Regulator in
Glioma
3
Jianhai Jiang and Jianxin Gu
1.
Overview
4
2.
Experimental
5
3.
Results
8
4.
Conclusion and Future Direction
18
Acknowledgments
22
References
22
2.
Roles of Polysialic Acid in Migration and Differentiation
of Neural Stem Cells
25
Kiyohiko Angata and
Minoru Fukuda
1.
Overview
26
Acknowledgments
34
References
34
3.
Structural and Functional Analysis of Chondroitin
Sulfate
Proteogiycans in the Neural Stem Cell Niche
37
Swetlana
Sirko, Kaoru
Akita, Alexander
Von Holst,
and Andreas Faissner
1.
Overview
39
2.
Immunohistochemistry of Embryonic and Postnatal Mouse
Brain Sections
42
3.
Method to Stain Embryonic Sections for NSPC Markers
43
4.
Analysis of the Adult Neurogenic Niche and SVZ-Derived Cells by
Immunocytochemistry
44
5.
Immunocytochemistry of Acutely Dissociated Cells
46
6.
Isolation of NSPCs by Immunopanning or by
Immunoisolation
Using
Paramagnetic Beads (EasySep)
47
v¡
Contents
7. Neurosphere
Cultures
and Various Methods for Their Analysis
49
8.
Disaccharide Analysis of CS/DS Chains from Embryonic Brain and
Conditioned Neurosphere Culture Media and Effect of Sodium Chlorate
Treatment on Neurosphere Formation at Clonal Cell Density
55
9.
Analysis of NSPC-Proliferation In Vitro and In Vivo
56
10.
Analysis of CSPG Functions in NSPCs Using Chondroitinase ABC
Treatment in Culture
56
11.
Analysis of Chondroitin
Sulfate
Functions in the Neural Stem
Cell Niche
58
12.
RT-PCR and
Semiquantitative
Analysis of the Synthetic Machinery
for Glycosaminoglycans
59
13.
In Situ Hybridization of Sulfotransferases in Tissue and Neurosphere
Sections
63
14.
Microscopy
66
15.
Conclusion and Outlook
66
Acknowledgments
66
References
66
4.
Transcript Analysis of Stem Cells
73
Alison V. Nairn, Mitche
dela
Rosa, and Kelley W. Moremen
1.
Introduction
74
2.
qRT-PCR as a Tool for Determining Transcript Analysis for
Glycan-Related Gene Expression
75
3.
Examples of the Applications of qRT-PCR Transcript Analysis to
Investigate Changes in Glycan-Related Gene Expression in Stem Cells
83
Acknowledgments
88
References
88
5.
Directing Stem Cell Trafficking via GPS
93
Robert
Sackstein
1.
Overview
94
2.
Rationale for GPS
95
3.
Guiding Principles and Method for GPS
97
4.
Method of aC^-Fucosylation of Cell Surface Using FTVI
99
5.
Detection of E-Selectin Ligand Expression Following GPS
100
6.
Testing for E-Selectin Ligand Activity Using E-Selectin-lg Chimera
(Three-Step Method for Flow Cytometry)
101
7.
Summary 102
Acknowledgments
ЮЗ
References
103
Contents
vii
6.
Functional Assays for the Molecular Chaperone Cosmc
107
Tongzhongju and Richard D. Cummings
1.
Overview
108
2.
T-Synthase and Cosmc
110
3.
T-Synthase Activity Assay
113
4.
Assay Activity for Function of Cosmc
116
5.
Conclusion and Future Directions
119
Acknowledgments
120
References
120
7.
Core S-Derived O-Glycans Are Essential for Intestinal
Mucus Barrier Function
123
Lijun Xia
1.
Introduction
124
2.
Generation of C3GnT h Mice
126
3.
Disruption of the C3GnT Gene Eliminates Core 3-Derived
O-Glycans and Exposes the Tn Antigen in
Murine
Colon
128
4.
Deficiency of C3GnT Results in Reduced Muc2 Expression in
Colon and Impaired Mucosal Integrity
131
5.
СЗЄпТ 1
Mice are Highly Susceptible to Dextran Sodium
Sulfate-lnduced Colitis
133
6.
Conclusion and Future Direction
138
Acknowledgments
140
References
140
8.
СогеЗ
Glycan as Tumor Suppressor
143
Seung Ho Lee and
Minoru Fukuda
1.
Overview
144
2.
Generating
СогеЗ
Glycan Expressing Cell Lines
145
3.
Detection Methods of
СогеЗ
Expression
146
4.
Migration Assay Using Attractant
147
5.
Determination of Major
Integrin
Using Functional
Blocking Antibodies
147
6.
Tumor Formation Assay
149
7.
Western Blotting and Lectin Blotting
150
8.
Heterodimerization Assay
151
9.
FAK
Signaling
152
References
153
v¡¡¡
Contents
9.
Characterization
of Mice with Targeted Deletion of the Gene
Encoding Core
2 ßl.o-W-Acetylglucosaminyltransferase-Z 155
Erica L. Stone, Seung Ho Lee, Mohd
Nazri
Ismail, and
Minoru Fukuda
1.
Introduction
156
2.
Generation and Genotyping of C2GnT2
KO
Mice
157
3.
Mice Lacking C2GnT2 Have Reduced Core
2
and no
Core
4
Enzyme Activity and Altered Glycosylation
159
4.
Phenotyping C2GnT2
KO
Mice
161
5.
Conclusions and Future Directions
168
Acknowledgments
170
References
170
10.
Analyzing Physiological Function of Polypeptide
GalNAcT-l-Deficient Mice in Humoral Immunity
173
Mari Tenno
1.
Overview
174
2.
Assay for In Vitro
В
Lymphocyte Activation
174
3.
Assay for In Vivo Antibody Production
175
4.
Immunohistochemical Staining of Frozen Sections
177
5.
Apoptosis Detection Using Antibody Against Caspase
3
Active Form
179
6.
Apoptosis Detection by
TUNEL
System
182
Acknowledgment
183
References
183
11.
j83GnT2 (B3GNT2), A Major Polylactosamine Synthase:
Analysis of
fôgntl-Defícient
Mice
185
Akira Togayachi, Yuko Kozono, Atsushi
Kuno,
Takashi Ohkura,
Takashi Sato,
Jun
Hirabayashi, Yuzuru
Ikehara,
and Hisashi Narimatsu
1.
Overview
186
2.
Glycogenes for Polylactosamine Synthesis (i.e.,
¿81,3-
N-Acetylglucosaminyltransferase Genes)
186
3.
N-Glycan Polylactosamine is Greatly Reduced in B3gnt2~^ Mice
187
4.
Phenotype of B3gnt2 ~ Lymphocytes Lacking
Polylactosamine on
Лѓ
-Glycans
193
5.
Protocols
197
Acknowledgments
202
References
202
Contents
¡χ
12.
Targeted Genetic Inactivation of /V-Acetylglucosaminyltransferase-
IVa Impairs Insulin Secretion from Pancreatic
β
Cells
and Evokes Type
2
Diabetes
205
Kazuaki Ohtsubo
1.
Overview
206
2.
Engineering GnT-IVa-Deficient Mice
207
3.
GnT-IV Enzymology
209
4.
Glucose and Insulin Homeostasis
210
5.
Immunohistochemical Analysis
211
6.
Islet Cell Preparation and Culture
214
7.
Pulse-Chase Labeling
215
8.
Cell Surface Half-Life Time of GLUT2
216
9.
GLUT2 Glycan Analysis by Lectin Blot
217
10.
Cell-Surface Protein Cross-Linking
218
Acknowledgment
220
References
220
13.
The Ashwell-Morell Receptor
223
Prabhjit K. Grewal
1.
Overview
224
2.
Endogenous Ligands of the AMR
227
3.
Hepatocytes in Molecular Clearance Mechanisms of the Liver
228
4.
Genotyping HL-1- or
HL-2-Deficient
Mice
229
5.
Hematology
and Coagulation Analyses Methods (Partly Adapted
from Ellies
er
al,
2002;
Grewal
et al,
2008;
Wang et al,
2001) 230
Acknowledgments
236
References
236
14.
Roles of
GlcNAc-ó-O-Sulfotransferases
in Lymphoid
and Nonlymphoid Tissues
243
Hiroto Kawashima
1.
Overview
244
2.
Establishment of a Mouse Colon Adherent Cell Line and Cell Culture
246
3.
Treatment of CAdCl Cells with SCFAs
247
4.
RT-PCR
247
5.
Histology and immunostaining
248
6.
Preparation of Colonic-Mucin-Enriched Fraction
249
7.
Carbohydrate Structural Analysis
250
8.
Induction of Colitis by Dextran
Sulfate
Sodium
254
Acknowledgments
255
References
255
Contents
15.
Core O-Glycans Required for Lymphocyte Homing: Gene
Knockout Mice of Core
1
/Jl^-Af-Acetylglucosaminyltransferase
and Core
2
W-Acetylglucosaminyltransferase
257
Junya Mitoma and
Minoru Fukuda
1.
Introduction
258
2.
Lymphocyte Homing Assay
260
3.
Staining of Lymph Nodes by L- and E-Selectin-IgM Chimeric
Proteins after Glycosidase Treatment
263
4.
Probing of L-Selectin Ligands on Membrane Filters with L- and
E-selectin-IgM Chimeric Proteins
265
5.
Conclusions
267
Acknowledgments
269
References
269
16.
Immunohistochemical Analysis of Carbohydrate Antigens
in Chronic Inflammatory Gastrointestinal Diseases
271
Motohiro Kobayashi and
Jun
Nakayama
1.
Overview
272
2.
Immunohistochemical Analysis Using Conventional Immunostaining
274
3.
Immunohistochemical Analysis Using Multiple Immunostaining
279
4.
Immunohistochemical Analysis Using L-SelectinlgM
Chimera Binding
283
Acknowledgments
287
References
287
17.
Genetic Defects in Muscular Dystrophy
291
Kumaran Chandrasekharan and Paul T. Martin
1.
Overview
292
2.
Mouse Models of Muscular Dystrophy
294
3.
Approach to Phenotype Analysis in Mouse Muscular
Dystrophy Models
306
4.
Summary
312
Acknowledgments
312
References
312
18.
POMT1 is Essential for Protein O-Mannosylation in Mammals
323
Mark
Lömmel,
Tobias Wilier,
Jesús Cruces,
and
Sabine Strahl
1.
Overview
324
2.
Experimental
325
3.
Discussion/Conclusions
337
Contents xi
Acknowledgments
339
References
340
19.
POMGnTl, POMT1, and POMT2 Mutations in Congenital
Muscular Dystrophies
343
Tamao Endo, Hiroshi
Manya,
Nathalie Seta, and
Pascale
Guicheney
1.
Overview
344
2.
Methods
345
3.
Procedures for Enzymatic Activity and Mutation Search
348
Acknowledgment
350
References
350
20.
Cellular and Molecular Characterization of Abnormal Brain
Development in Protein O-Mannose
/V-AcetylgLucosaminyltransferase
1
Knockout Mice
353
Jianmin Liu, Yuan Yang, Xiaofeng Li, Peng Zhang, Yue Qi,
and Huaiyu Hu
1.
Overview
354
2.
Analysis of a-DG Glycosylation and Laminin Binding by Western Blot
355
3.
Histological Analysis of POMGnTl Knockout Brain
356
4.
Lamination Defects in the Neocortex of POMGnTl Knockout Mice
357
5.
Analysis of the
Pial
Basement Membrane by Laminin Immunostaining
359
6.
Analysis of the
Pial
Basement Membrane by Transmission Electron
Microscopy
360
7.
Analysis of the
Glia
Limitans
by GFAP Immunofluorescence Staining
362
Acknowledgment
364
References
365
21.
Investigating the Functions of LARGE: Lessons from Mutant Mice
367
Jane E. Hewitt
1.
Overview
368
2.
Human LARGE and Relevance to Disease
369
3.
Identification of Mice Carrying Mutations in Large
370
4.
Loss of Functional Large Protein Results in Hypoglycosylation
of a-Dystroglycan
373
5.
Phenotypes of Mice with Mutations in Large
374
6.
Expression of LARGE Genes
378
7.
Does Large Encode a Functional Glycosyltransferase?
379
8.
Largé ^
Mice as a Model for Therapeutic Approaches to
Dystroglycanopathy
381
Acknowledgments
381
References
381
xii Contents
22.
A Tumor Suppressor Function of Laminin-Binding
a-Dystroglycan
387
Xingfeng
Bao
and
Minoru Fukuda
1.
Background
388
2.
Methods
389
Acknowledgment
395
References
395
23.
Tumor Formation Assays
397
Shingo Hatakeyama, Hayato Yamamoto, and Chikara Ohyama
1.
Overview
398
2.
Animal Care and Protocol Approval
399
3.
Disinfection of Mice
399
4.
Analgesia and Anesthesia in Mice
399
5.
IP Injection
401
6.
IV Injection into the Tail Vein
401
7.
IV Tumor Formation Assay Using Immune-Deficiency Mice
403
8.
SC Inoculation
405
9.
FP Inoculation
407
10.
Testicular Inoculation
408
11.
Prostate Inoculation
408
Acknowledgment
411
References
411
Author Index
413
Subject Index
439
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| genre | (DE-588)4143413-4 Aufsatzsammlung gnd-content |
| genre_facet | Aufsatzsammlung |
| id | DE-604.BV036793370 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T22:48:21Z |
| institution | BVB |
| isbn | 9780123809971 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-020709733 |
| oclc_num | 700444990 |
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| owner_facet | DE-91G DE-BY-TUM DE-355 DE-BY-UBR DE-19 DE-BY-UBM DE-12 |
| physical | XLVIII, 447 S. Ill., graph. Darst. |
| publishDate | 2010 |
| publishDateSearch | 2010 |
| publishDateSort | 2010 |
| publisher | Elsevier, Acad. Press |
| record_format | marc |
| series | Methods in enzymology |
| series2 | Methods in enzymology |
| spelling | Functional glycomics ed. by Minoru Fukuda 1. ed. Amsterdam [u.a.] Elsevier, Acad. Press 2010 XLVIII, 447 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in enzymology 479 Labortechnik (DE-588)4123602-6 gnd rswk-swf Glykobiologie (DE-588)7554628-0 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Glykobiologie (DE-588)7554628-0 s Labortechnik (DE-588)4123602-6 s b DE-604 Fukuda, Minoru 1945- Sonstige (DE-588)132497719 oth Methods in enzymology 479 (DE-604)BV000000938 479 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020709733&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Functional glycomics Methods in enzymology Labortechnik (DE-588)4123602-6 gnd Glykobiologie (DE-588)7554628-0 gnd |
| subject_GND | (DE-588)4123602-6 (DE-588)7554628-0 (DE-588)4143413-4 |
| title | Functional glycomics |
| title_auth | Functional glycomics |
| title_exact_search | Functional glycomics |
| title_full | Functional glycomics ed. by Minoru Fukuda |
| title_fullStr | Functional glycomics ed. by Minoru Fukuda |
| title_full_unstemmed | Functional glycomics ed. by Minoru Fukuda |
| title_short | Functional glycomics |
| title_sort | functional glycomics |
| topic | Labortechnik (DE-588)4123602-6 gnd Glykobiologie (DE-588)7554628-0 gnd |
| topic_facet | Labortechnik Glykobiologie Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020709733&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV000000938 |
| work_keys_str_mv | AT fukudaminoru functionalglycomics |