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PCR protocols:

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Bibliographic Details
Format:	Book
Language:	English
Published:	New York Humana Press / Springer 2011
Edition:	3. ed
Series:	Methods in molecular biology 687
Subjects:	Polymerase-Kettenreaktion
Online Access:	Inhaltsverzeichnis
Physical Description:	XI, 348 S. Ill., graph. Darst.
ISBN:	9781607619437

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adam_text	IMAGE 1 PCR PROTOCOLS THIRD EDITION EDITED BY DANIEL J. PARK GENETIC EPIDEMIOLOGY LABORATORY, DEPARTMENT OFPATHOIOGY, THE UNIVERSITY OF MELBOURNE, PARKVILLE, VIC, AUSTRALIA % HUMANA PRESS IMAGE 2 CONTENTS PREFACE V CONTRIBUTORS IX PART I P CR HUSBANDRY 1 SETUP OF A PCR LABORATORY 3 ZAHEER KHAN PART II CLONING AND SEQUENCING 2 LONG-RANGE PCR WITH A DNA POLYMERASE FUSION 17 HOLLY H. HOGREFE AND MICHAEL C. BOMS 3 ISOLATION OF GENOMIC INSERTION SITES OF PROVIRUSES USING SPLINKERETTE-PCR-BASED PROCEDURES 25 BIN YIN 4 LARIAT-DEPENDENT NESTED PCR FOR FLANKING SEQUENCE DETERMINATION 43 DANIEL J. PARK 5 CODEHOP PCR AND CODEHOP PCR PRIMER DESIGN 57 JEANNETTE P. STAHELI, RICHARD BOYCE, DINA KOVARIK, AND TIMOTHY M. ROSE 6 SEQUENCING OF DIFFICULT DNA REGIONS BY SAM SEQUENCING 75 KEITH R. MITCHELSON PART III EXPRESSION, EPIGENETIC AND COPY NUMBER ANALYSIS 7 A GLOBAL SINGLE-CELL CDNA AMPLIFICATION METHOD FOR QUANTITATIVE MICROARRAY ANALYSIS 91 KAZUKI KURIMOTO AND MITINORI SAITOU 8 QUANTITATION OF MICRORNAS BY REAL-TIME RT-QPCR 113 CAIFU CHEN, RUOYINJJ TAN, LINDA WONG, RICHARD FEKETE, AND JASON HALSEY 9 HIGH-THROUGHPUT SUPERSAGE 135 HIDEO MATSUMURA, KENTARO YOSHIDA, SHUJUN LUO, DETLEV H. KRIIGER, GIINTER KAHL, GARY P. SCHROTH, AND RYOHEI TERAUCHI 10 DEEP CAP ANALYSIS OF GENE EXPRESSION 147 JUNPEI KUROSAWA, HIROMI NISHIYORI, AND YOSHIHIDE HAYASHIZAKI 11 LINKING EMULSION PCR HAPLOTYPE ANALYSIS 165 JAMES G. WETTNUR ANDJIA CHEN VII IMAGE 3 VIII CONTENTS 12 PAP-LMPCR: AN IMPROVED, SEQUENCE-SELECTIVE METHOD FOR THE IN VIVO ANALYSIS OF TRANSCRIPTION FACTOR OCCUPANCY AND CHROMATIN FINE STRUCTURE 177 RICHARD INGRAM, ARTHUR RIGGS, AND CONSTANZE BONIFER 13 THE MANY FACES OF MLPA 193 THOMAS OHNESORG, ERIN TURBITT, AND STEFAN J. WHITE 14 ASSESSING GENE-SPECIFIC METHYLATION USING HRM-BASED ANALYSIS 207 EE MING WONG AND ALEXANDER DOBROVIC PART IV PROBES AND ASSAYS 15 ALU PCR 221 MAURIZIO CARDELLI 16 ASYNCHRONOUS PCR 231 CAIFU CHEN, DAVID RUFF, AND JASON HALSEY 17 NOVEL APPLICATIONS OF PCR THROUGH THE USE OF DNA SUBSTRATES 245 STUART M. WILSON 18 ENHANCED SOLID PHASE PCR FOR INCREASED LOADING OF AMPLICON ONTO SOLID SUPPORT 257 DANIEL J. PARK 19 APPLICATION OF BLOCKING OLIGONUCLEOTIDES TO IMPROVE SIGNAL-TO-NOISE RATIO IN A PCR 265 HEGE VESTHEIM, BRUCE E. DEAGLE, AND SIMON N. JARTNAN PART V ENGINEERING AND SCREENING 20 ASYMMETRIC OVERLAP EXTENSION PCR METHOD FOR SITE-DIRECTED MUTAGENESIS 277 YUE-HUA XIAO AND YAN PEI 21 RIBOSOME DISPLAY: A TECHNOLOGY FOR SELECTING AND EVOLVING PROTEINS FROM LARGE LIBRARIES 283 BIRGIT DREIER AND ANDREAS PLIICKTHUN 22 GLOBE: ANALYSIS OF DNA-PROTEIN INTERACTION ANALYSIS 307 TAKAAKI KOJIMA AND HIDEO NAKANO 23 PCR DNA-ARRAY PROFILING OF DNA-BINDING TRANSCRIPTION FACTOR ACTIVITIES IN ADULT MOUSE TISSUES 319 YIMIN SUN, JING CHENG, AND KEITH R. MITCHELSON 24 NUCLEOTIDE EXCHANGE AND EXCISION TECHNOLOGY DNA SHUFFLING AND DIRECTED EVOLUTION 333 JANINA SPECK, SABINE C. STEBEL, KATJA M. ARNDT, AND KRISTIAN M. MILLLER INDEX 345
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discipline	Biologie
edition	3. ed
format	Book
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indexdate	2024-07-09T22:46:39Z
institution	BVB
isbn	9781607619437
language	English
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owner	DE-20
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physical	XI, 348 S. Ill., graph. Darst.
publishDate	2011
publishDateSearch	2011
publishDateSort	2011
publisher	Humana Press / Springer
record_format	marc
series	Methods in molecular biology
series2	Methods in molecular biology
spelling	PCR protocols ed. by Daniel J. Park 3. ed New York Humana Press / Springer 2011 XI, 348 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 687 Polymerase-Kettenreaktion (DE-588)4256726-9 gnd rswk-swf Polymerase-Kettenreaktion (DE-588)4256726-9 s DE-604 Park, Daniel J. Sonstige oth Methods in molecular biology 687 (DE-604)BV035362695 687 HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020642554&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
spellingShingle	PCR protocols Methods in molecular biology Polymerase-Kettenreaktion (DE-588)4256726-9 gnd
subject_GND	(DE-588)4256726-9
title	PCR protocols
title_auth	PCR protocols
title_exact_search	PCR protocols
title_full	PCR protocols ed. by Daniel J. Park
title_fullStr	PCR protocols ed. by Daniel J. Park
title_full_unstemmed	PCR protocols ed. by Daniel J. Park
title_short	PCR protocols
title_sort	pcr protocols
topic	Polymerase-Kettenreaktion (DE-588)4256726-9 gnd
topic_facet	Polymerase-Kettenreaktion
url	http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020642554&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
volume_link	(DE-604)BV035362695
work_keys_str_mv	AT parkdanielj pcrprotocols

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