PCR protocols:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York
Humana Press / Springer
2011
|
| Ausgabe: | 3. ed |
| Schriftenreihe: | Methods in molecular biology
687 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XI, 348 S. Ill., graph. Darst. |
| ISBN: | 9781607619437 |
Internformat
MARC
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Datensatz im Suchindex
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|---|---|
| adam_text | IMAGE 1
PCR PROTOCOLS
THIRD EDITION EDITED BY
DANIEL J. PARK
GENETIC EPIDEMIOLOGY LABORATORY, DEPARTMENT OFPATHOIOGY, THE UNIVERSITY
OF MELBOURNE, PARKVILLE, VIC, AUSTRALIA
% HUMANA PRESS
IMAGE 2
CONTENTS
PREFACE V
CONTRIBUTORS IX
PART I P CR HUSBANDRY
1 SETUP OF A PCR LABORATORY 3
ZAHEER KHAN
PART II CLONING AND SEQUENCING
2 LONG-RANGE PCR WITH A DNA POLYMERASE FUSION 17
HOLLY H. HOGREFE AND MICHAEL C. BOMS 3 ISOLATION OF GENOMIC INSERTION
SITES OF PROVIRUSES USING SPLINKERETTE-PCR-BASED PROCEDURES 25
BIN YIN 4 LARIAT-DEPENDENT NESTED PCR FOR FLANKING SEQUENCE
DETERMINATION 43 DANIEL J. PARK 5 CODEHOP PCR AND CODEHOP PCR PRIMER
DESIGN 57
JEANNETTE P. STAHELI, RICHARD BOYCE, DINA KOVARIK, AND TIMOTHY M. ROSE 6
SEQUENCING OF DIFFICULT DNA REGIONS BY SAM SEQUENCING 75
KEITH R. MITCHELSON
PART III EXPRESSION, EPIGENETIC AND COPY NUMBER ANALYSIS
7 A GLOBAL SINGLE-CELL CDNA AMPLIFICATION METHOD FOR QUANTITATIVE
MICROARRAY ANALYSIS 91
KAZUKI KURIMOTO AND MITINORI SAITOU 8 QUANTITATION OF MICRORNAS BY
REAL-TIME RT-QPCR 113
CAIFU CHEN, RUOYINJJ TAN, LINDA WONG, RICHARD FEKETE, AND JASON HALSEY 9
HIGH-THROUGHPUT SUPERSAGE 135
HIDEO MATSUMURA, KENTARO YOSHIDA, SHUJUN LUO, DETLEV H. KRIIGER, GIINTER
KAHL, GARY P. SCHROTH, AND RYOHEI TERAUCHI 10 DEEP CAP ANALYSIS OF GENE
EXPRESSION 147
JUNPEI KUROSAWA, HIROMI NISHIYORI, AND YOSHIHIDE HAYASHIZAKI 11 LINKING
EMULSION PCR HAPLOTYPE ANALYSIS 165
JAMES G. WETTNUR ANDJIA CHEN
VII
IMAGE 3
VIII CONTENTS
12 PAP-LMPCR: AN IMPROVED, SEQUENCE-SELECTIVE METHOD FOR THE IN VIVO
ANALYSIS OF TRANSCRIPTION FACTOR OCCUPANCY AND CHROMATIN FINE STRUCTURE
177
RICHARD INGRAM, ARTHUR RIGGS, AND CONSTANZE BONIFER
13 THE MANY FACES OF MLPA 193
THOMAS OHNESORG, ERIN TURBITT, AND STEFAN J. WHITE 14 ASSESSING
GENE-SPECIFIC METHYLATION USING HRM-BASED ANALYSIS 207
EE MING WONG AND ALEXANDER DOBROVIC
PART IV PROBES AND ASSAYS
15 ALU PCR 221
MAURIZIO CARDELLI 16 ASYNCHRONOUS PCR 231
CAIFU CHEN, DAVID RUFF, AND JASON HALSEY 17 NOVEL APPLICATIONS OF PCR
THROUGH THE USE OF DNA SUBSTRATES 245 STUART M. WILSON 18 ENHANCED SOLID
PHASE PCR FOR INCREASED LOADING OF AMPLICON
ONTO SOLID SUPPORT 257
DANIEL J. PARK 19 APPLICATION OF BLOCKING OLIGONUCLEOTIDES TO IMPROVE
SIGNAL-TO-NOISE RATIO IN A PCR 265
HEGE VESTHEIM, BRUCE E. DEAGLE, AND SIMON N. JARTNAN
PART V ENGINEERING AND SCREENING
20 ASYMMETRIC OVERLAP EXTENSION PCR METHOD FOR SITE-DIRECTED MUTAGENESIS
277
YUE-HUA XIAO AND YAN PEI 21 RIBOSOME DISPLAY: A TECHNOLOGY FOR SELECTING
AND EVOLVING PROTEINS FROM LARGE LIBRARIES 283
BIRGIT DREIER AND ANDREAS PLIICKTHUN 22 GLOBE: ANALYSIS OF DNA-PROTEIN
INTERACTION ANALYSIS 307
TAKAAKI KOJIMA AND HIDEO NAKANO 23 PCR DNA-ARRAY PROFILING OF
DNA-BINDING TRANSCRIPTION FACTOR ACTIVITIES IN ADULT MOUSE TISSUES 319
YIMIN SUN, JING CHENG, AND KEITH R. MITCHELSON 24 NUCLEOTIDE EXCHANGE
AND EXCISION TECHNOLOGY DNA SHUFFLING AND DIRECTED EVOLUTION 333
JANINA SPECK, SABINE C. STEBEL, KATJA M. ARNDT, AND KRISTIAN M. MILLLER
INDEX 345
|
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| id | DE-604.BV036724748 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T22:46:39Z |
| institution | BVB |
| isbn | 9781607619437 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-020642554 |
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| physical | XI, 348 S. Ill., graph. Darst. |
| publishDate | 2011 |
| publishDateSearch | 2011 |
| publishDateSort | 2011 |
| publisher | Humana Press / Springer |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | PCR protocols ed. by Daniel J. Park 3. ed New York Humana Press / Springer 2011 XI, 348 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 687 Polymerase-Kettenreaktion (DE-588)4256726-9 gnd rswk-swf Polymerase-Kettenreaktion (DE-588)4256726-9 s DE-604 Park, Daniel J. Sonstige oth Methods in molecular biology 687 (DE-604)BV035362695 687 HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020642554&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | PCR protocols Methods in molecular biology Polymerase-Kettenreaktion (DE-588)4256726-9 gnd |
| subject_GND | (DE-588)4256726-9 |
| title | PCR protocols |
| title_auth | PCR protocols |
| title_exact_search | PCR protocols |
| title_full | PCR protocols ed. by Daniel J. Park |
| title_fullStr | PCR protocols ed. by Daniel J. Park |
| title_full_unstemmed | PCR protocols ed. by Daniel J. Park |
| title_short | PCR protocols |
| title_sort | pcr protocols |
| topic | Polymerase-Kettenreaktion (DE-588)4256726-9 gnd |
| topic_facet | Polymerase-Kettenreaktion |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020642554&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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