Microtubules, in vitro:
Gespeichert in:
Format: | Buch |
---|---|
Sprache: | English |
Veröffentlicht: |
Amsterdam [u.a.]
Elsevier, Acad. Press
2010
|
Ausgabe: | 1. ed. |
Schriftenreihe: | Methods in cell biology
95 |
Schlagworte: | |
Online-Zugang: | Inhaltsverzeichnis |
Beschreibung: | 2. Aufl. u.d.T.: Microtubules, in vitro / John J. Correia |
Beschreibung: | XXI, 700, [32] S. Ill., graph. Darst. |
ISBN: | 9780123748157 |
Internformat
MARC
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245 | 1 | 0 | |a Microtubules, in vitro |c ed. by Leslie Wilson ... |
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300 | |a XXI, 700, [32] S. |b Ill., graph. Darst. | ||
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Datensatz im Suchindex
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---|---|
adam_text | CONTENTS
Contributors
Preface
SECTION I Isolation and Biochemistry of Tubulin and
Characterization of Antibodies and
Isotypes
Preparation of Microtubule Protein and Purified Tubulin from Bovine Brain
by Cycles of Assembly and Disassembly and Phosphocellulose Chromatography
Herbert P. Miller and Leslie Wilson
I. Introduction
3
II. Protocols
4
III. Buffer Compositions
13
IV. Concluding Comments
14
References
14
2.
Isolating Tubulin from
Nonneural
Sources
Dan L. Sackett, Karl A. Werbovetz, and Naomi S. Morrissette
I. Why Tubulin from
Nonneural
Sources?
18
II. General Principles of Tubulin Purification
18
III. Specific Properties of Distinct
Nonneural
Sources
19
IV. Genetic Manipulation of Tubulin Genes
22
V. Isolation of Tubulin: Theme and Variation
24
VI. Protocol Notes
28
VII.
Summary
29
References
29
3.
Characterization of
Anti-ß-tubulin
Antibodies
Anthony J.
Spano
and Anthony Frankfurter
I. Introduction
33
II. The Characterization of AA2, a Pan-specific
Anti-ß-tubulin
Monoclonal Antibody That Reacts with All Vertebrate
ß-Tubulin
Isotypes
(Gene Products)
34
III. The Characterization of Isotype-Specific
ß-Tubulin
Monoclonal
and Polyclonal Antibodies
36
IV. The Characterization of Antibodies That Recognize the Glutamyl
Side Chain of Glutamylated Proteins
37
V. Summary
44
References
44
Contents
Expression
Profiling
of
Tubulin
Isotypes and
Microtubule-Interacting
Proteins Using
Real-Time Polymerase
Chain Reaction
Sharon
Lobért,
Laree Hiser, and John J.
Сотій
I. Introduction and Rationale
48
II. Methods and Materials
48
III. Results and Discussion
54
IV. Summary
58
References
58
5.
Nondenaturing
Ełectrophoresis
as a Tool to Investigate Tubulin Complexes
Monica
López Fanarraga, Gerardo Carranza, Raquel Castaño,
Sofia
Nolasco,
J.
Avila,
and]. C.
Zăbala
I. Introduction
60
II. Rationale
60
III. Methods
61
IV. Materials
72
V. Discussion
73
VI. Summary
73
References
74
6.
Mass Spectrometry Analysis of C-Terminal Posttranslational Modifications
of Tubulins
Virginie
Redeker
I. Introduction
78
II. Methods
82
III. Results and Discussion
87
IV. Conclusion
98
References
99
7.
Methods in Tubulin Proteomics
Leah M. Miller,
Hui
Xiao,
Berta Burd,
Susan Band Horwitz,
Ruth Hogue Angeletti, and Pascal Verdier-Pinard
I. Introduction
106
II. Methods
107
III. Summary
123
References
124
SECTION II Microtubule Structure and Dynamics
8.
Cryo-EM Studies of Microtubule Structural Intermediates
and Kinetochore—Microtubule Interactions
Eva S ogales, Vincent H. Ramey, and Hong-Wei Wang
I. Introduction
130
II. Rationale
134
Contents
III. Methods
138
IV. Discussion
149
V. Summary
152
References
153
9.
High-Resolution Imaging of Microtubules and Cytoskeleton
Structures by Atomic Force Microscopy
Loie
Нйтоп,
Patrick
A. Curmi,
and David Pastr e
I. Introduction to AFM Imaging of Biomolecules
158
II. Rationale
159
III. AFM Principle and Operation Mode
159
IV. Practical Aspects of Microtubules Adsorption on Surface
and AFM Imaging
161
V. AFM Images in Air of Microtubule
167
VI. AFM Imaging in Air ofTubulin or MicrotubulerProtein Interactions
169
VII.
Conclusion
171
References
172
10.
Using Computational Modeling to Understand Microtubule Dynamics:
A Primer for Cell Biologists
Holly V. Goodson and Ivan V. Gregoretti
I. Introduction
176
II. Mathematical and Computational Modeling: A Primer
177
III. Using Modeling to Understand Microtubule Dynamics
181
IV. Conclusions
186
References
187
11.
Analysis of Dynamic Instability of Steady-State Microtubules In Vitro
by Video-Enhanced Differential Interference Contrast Microscopy
with an Appendix by
Emin Oroudjev
Xlythili Yeiijerla,
Manu
Lopus, and Leslie Wilson
I. Introduction
190
II. Method
192
III. Summan-
and Conclusion
200
References
201
Appendix
203
I. Introduction
203
II. MT-LHAP and Its Features
204
III. Instructions to Use MT-LHAP
204
IV. Conclusion
206
References
206
12.
Nanometer-Resoiution Microtubule Polymerization Assays Using Optical
Tweezers and
Microfabricated
Barriers
Blake D. Chartehois, Henry T. Schek HI, and AlanJ. Hunt
I. Introduction
208
II. Rationale
209
Contents
III. Methods
209
IV. Materials
212
V. Discussion
214
VI. Summary
217
References
217
13.
Microtubule Dynamics Reconstituted In Vitro and Imaged
by Single-Molecule Fluorescence Microscopy
Christopher
Gell, Volker
Bormuth,
Cary
J. Brouhard,
Daniel
N.
Cohen,
Stefan
Diez,
Claire T. Friel,
Jonne
Helenius,
Bert Nitzsche, Heike Petzold,
Jan Ribbe,
Erik Schiffer,
Jeffrey H. Stear, Anastasiya Trushko, Vladimir
Varga,
Per O. Widlund,
Marija
Zanic, and Jonathon Howard
I. Introduction
222
II. Single-Molecule TIRF Microscopy
224
III. List of Reagents
227
IV. Choice of Fluorophore/Protein Labeling
229
V. (Anti^blinking/Photo-Toxicity/Photo-Bleaching Cocktails
230
VI. Preparation of GMPCPP-Stabilized Microtubules
233
VII.
Glass Treatment and Sample Chamber Preparation
236
VIII.
Binding of Microtubules and Passivation of Surfaces
239
IX. Dynamic Microtubule Assays
241
References
243
14.
Studying Kinesin Motors by Optical SD-Nanometry in Gliding Motility
Assays
Bert Nitzsche,
Volker
Bormuth,
Corina
Bräuer,
Jonathon Howard, Leonid lonov,
Jacob Kerssemakers, Till
Korten, Cecile
Leduc,
Felix Ruhnow, and Stefan
Diez
I. Introduction
248
II. Setup of Gliding Motility Assays
249
III. Analysis of Microtubule and Quantum Dot Movements
259
IV. Future Directions
266
Reagents
267
References
269
SECTION III Drugs
15.
Analysis of Tubulin
Oligomers
by Analytical Ultracentrirugation
JolmJ.
Correia
I. Introduction
276
II. Materials and Methods
276
III. Results and Discussion
282
IV. Summary
286
References
286
Contents
16. Determination
of Drug Binding to Microtubules In Vitro
Jennifer A. Smith and Mary Ann Jordan
I. Introduction
290
II. Methods
292
III. Materials
298
References
298
17.
Fluorescence Spectroscopic Methods to Analyze Drug-Tubulin Interactions
Bhabatarak Bhattacharyya,
Sonia
Kapoor, and Dulal Panda
I. Introduction
302
II. Colchicine Binding to Tubulin
303
III. Vinblastine Binding to Tubulin
310
IV. Taxol Binding to Microtubules
316
V. Determination of Binding Constants Using Extrinsic Fluorescent
Probes
324
VI. Conclusion
325
References
326
18.
A Tubulin Polymerization Microassay Used to Compare Ligand Efficacy
Ashley Davis, Sarah Martinez, Daniel Nelson, and Kim Middleton
I. Introduction
332
II. Rationale
333
III. Methods
342
IV. Summary
349
References
350
19.
Fluorescent Taxoid Probes for Microtubule Research
babel Barasoain, J. Fernando Diaz, and
José M.
Andreu
I. Introduction
354
II. Materials
355
III. Methods
355
IV. Applications and Discussion
362
V. Summary
368
References
370
20.
The Binding of
Vinca
Domain Agents to Tubulin: Structural
and Biochemical Studies
Anthony Cormier, Marcel Knossow, Chunguang Wang, and
Benoît
Gigant
I. Introduction
374
II. Rationale
375
III. Structural Studies of the Tubulin-Vinca Domain Ligand Interactions
376
IV. Fluorescence Is Useful to Monitor Binding of
Vinca
Domain Ligands
to Tubulin in Solution
382
V. Effects of
Vinca
Domain Ligands on Tubulin Biochemical Properties
383
VI. Discussion
387
References
388
Contents
21.
Measurement of
Ligand
Binding to Tubulin by Sulfhydryl Reactivity
Adrian
Bégaye
and Dan L. Sackett
I. Introduction and Rationale
392
II. Methods
393
III. Discussion
401
References
402
SECTION IV Interactions with Motors and MAPs
22.
Probing Interactions of Tubulin with Small Molecules, Peptides, and
Protein Fragments by Solution Nuclear Magnetic Resonance
Marie-Jeanne Clement, Philippe
Savarin,
Elisabeth Adjadj,
André Sobel,
Flavio
Toma,
and Patrick
A. Curmi
I. Introduction
408
II. Rationale
409
III. Methods
414
IV. Application to Tubulin/Microtubules Interactions
422
V. Conclusion
441
VI. Appendix I.
Materials 441
References
444
23.
Microtubule and MAPs: Thermodynamics of Complex Formation
by AUC,
ITC,
Fluorescence, and NMR
François
Dewed,
Pascale Barbier,
Daniel Lafitte,
Isabelle Landrieu,
Guy
Lippens,
and Vincent Peyrot
I. Introduction
450
II. Rationale
452
III. Materials and Methods
456
IV. Discussion
463
V. Concluding Remarks
474
References
475
24.
Quantitative Analysis of MAP-Mediated Regulation of Microtubule Dynamic
Instability In Vitro
—
Focus on
Tau
Erkan Kiris, Donovan Ventimiglia, and Stuart C.
Feinstein
I. Introduction and Rationale
482
II. Methods
490
III. Materials
495
IV. Summary
497
References
498
25.
Structure and Dynamics of the Kinesin-Microtubule Interaction
Revealed by Fluorescence Polarization Microscopy
Hernando Sosa,
Ana
В.
Asenjo, and EnvinJ.
G. Peterman
I. Introduction
506
H.
Rationale
507
Contents
III. Methods
508
IV. Fluorescent Labeling for FPM
516
V. Discussion and Future Directions
517
References
518
26.
Multiple Color Single Molecule TIRF Imaging and Tracking of MAPs
and Motors
Jennifer L. Ross and Ram Dixit
I. Introduction
522
II. TIRF Optics
523
III. Labeling Molecules
528
IV. Examples and Protocols
528
V. Conclusions and Outlook
540
References
541
27.
Studying Plus-End Tracking at Single Molecule Resolution Using
TIRF Microscopy
Ram Dixit and Jennifer L. Ross
I. Introduction
544
II. Rationale
545
III. Methods
546
IV. Discussion
553
References
553
28.
Fluorescence Microscopy Assays on Chemically Functionalized Surfaces
for Quantitative Imaging of Microtubule, Motor, and +TIP Dynamics
Peter Bieling,
Ivo
A. Telley, Christian Hentrich, Jacob Piehler, and Thomas Suney
I. Introduction
556
II. Rationale
557
III. Materials
558
IV. Methods
559
V. Surface Chemistry on Glass
561
VI. Fluorescence Microscopy Assays
565
VII.
Discussion
576
VIII.
Conclusion
577
References
578
SECTION V Functional Extracts and Force Measurements
29.
Quantitative Characterization of Filament Dynamics by Single-Molecule
Lifetime Measurements
Leonid A. Mirny and Daniel j. Needleman
I. Introduction to Cytoskeletal Filament Dynamics
584
II. Single-Molecule Lifetime Measurements
585
Contents
III. Theoretical Foundations
587
IV. Results and Conclusion
597
References
599
30.
Extracting the Mechanical Properties of Microtubules from Thermal
Fluctuation Measurements on an Attached Tracer Particle
Katja
M.
Taute,
Francesco Patnpaloni, and
Ernst-Ludwig
Fiorin
I. Introduction
602
II. Rationale
604
III. Materials
606
IV. Methods
607
V. Discussion
613
VI. Summary
614
References
614
31.
In Vitro Assays to Study Force Generation at Dynamic Microtubule Ends
Liedetvij
Laan
and
Marileen Dogterom
I. Introduction
618
II. Materials
620
III. Methods
621
IV. Results
633
V. Conclusion/Discussion
634
References
636
32.
Reconstitution
and Functional Analysis of Kinetochore
Subcomplexes
Daniei
R.
Gestaut,
Jeremy Cooper, Charles
L.
Asbury, Trisha
N.
Davis, and
Linda
Wordeman
I. Introduction
642
II. Methods
642
III. Conclusion
656
References
656
33.
In Vitro Assays to Study the Tracking of Shortening Microtubule Ends
and to Measure Associated Forces
Ekaterina L. Grishchuk and Fazly I. Ataullakhanov
I. Introduction
658
II. Rationale
659
Ш.
Materials and Methods
660
IV. Summary and Discussion
673
References
674
index
677
Volume in Series
693
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id | DE-604.BV036619708 |
illustrated | Illustrated |
indexdate | 2024-07-09T22:44:20Z |
institution | BVB |
isbn | 9780123748157 |
language | English |
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owner_facet | DE-355 DE-BY-UBR |
physical | XXI, 700, [32] S. Ill., graph. Darst. |
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publisher | Elsevier, Acad. Press |
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spelling | Microtubules, in vitro ed. by Leslie Wilson ... 1. ed. Amsterdam [u.a.] Elsevier, Acad. Press 2010 XXI, 700, [32] S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in cell biology 95 2. Aufl. u.d.T.: Microtubules, in vitro / John J. Correia Mikrotubulus (DE-588)4169864-2 gnd rswk-swf Mikrotubulus (DE-588)4169864-2 s DE-604 Wilson, Leslie Sonstige oth Methods in cell biology 95 (DE-604)BV002534878 95 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020539761&sequence=000004&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
spellingShingle | Microtubules, in vitro Methods in cell biology Mikrotubulus (DE-588)4169864-2 gnd |
subject_GND | (DE-588)4169864-2 |
title | Microtubules, in vitro |
title_auth | Microtubules, in vitro |
title_exact_search | Microtubules, in vitro |
title_full | Microtubules, in vitro ed. by Leslie Wilson ... |
title_fullStr | Microtubules, in vitro ed. by Leslie Wilson ... |
title_full_unstemmed | Microtubules, in vitro ed. by Leslie Wilson ... |
title_short | Microtubules, in vitro |
title_sort | microtubules in vitro |
topic | Mikrotubulus (DE-588)4169864-2 gnd |
topic_facet | Mikrotubulus |
url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020539761&sequence=000004&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
volume_link | (DE-604)BV002534878 |
work_keys_str_mv | AT wilsonleslie microtubulesinvitro |