Protein chromatography:
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | Undetermined |
| Veröffentlicht: |
New York [u.a.]
Humana Press
2011
|
| Schriftenreihe: | Methods in molecular biology
681 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | X, 527 S. Ill., graph. Darst. |
| ISBN: | 9781607619123 9781607619130 |
Internformat
MARC
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| 245 | 1 | 0 | |a Protein chromatography |c ed. by Dermot Walls ... |
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| 300 | |a X, 527 S. |b Ill., graph. Darst. | ||
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Datensatz im Suchindex
| _version_ | 1804143168788103168 |
|---|---|
| adam_text | Contents
Preface
.................................................................
γ
Contributors
............................................................. ix
Part I Overviews
1
A Digest of Protein Purification
...................................... 3
Dermot Walls, Robert McGrath, and
Sinéad T.
Loughran
2
Gel-Filtration Chromatography
...................................... 25
darán O Fágáin,
Philip
M.
Cummins, and Brendan P. O Connor
3
Immunoaffinity Chromatography
..................................... 35
Jenny Fitzgerald, Paul Leonard, Elaine Darcy, and Richard O Kennedy
4
Avoiding Proteolysis During Protein Chromatography
..................... 61
Barry J. Ryan
5
Scale-Up of Protein Purification: Downstream Processing Issues
.............. 73
John Joseph Milne
6
Phage Display: A Powerful Technology for the Generation
of High Specificity Affinity Reagents from Alternative Immune Sources
........ 87
William
J.J
.
Finlay, Laird Bloom, and
Orla
Cunningham
7
Engineering Protein Stability
........................................ 103
Ciarán O Fágáin
8
Microfluidics in Protein Chromatography
............................... 137
Prank A. Gomez
9
Tagging
Recombinant
Proteins to Enhance Solubility and Aid Purification
...... 151
Dermot Walls and
Sinéad T.
Loughran
Part II Protocols
10
Storage and
Lyophilisation
of Pure Proteins
............................. 179
Ciarán O Fágáin
11
Differential Precipitation and Solubilization of Proteins
.................... 203
Barry
J
.
Ryan
12
Ion-Exchange Chromatography: Basic Principles and Application
to the Partial Purification of Soluble Mammalian Prolyl Oligopeptidase
......... 215
Philip M. Cummins, Oonagh
Bowling,
and Brendan F. O Connor
13
Protein Quantitation and Analysis of Purity
............................. 229
Eva M. Campion,
Sinéad
T.
Loughran, and Dermot Walls
14
Purification of Proteins Fused to Glutathione S-Transferase
................. 259
Sandra Harper and David W.
Speicher
15
Purification of Proteins Fused to Maltose-Binding Protein
.................. 281
Mario Lebendiker and Tsafi
Danieli
vil
viii Contents
16
Purification
of
Proteins
from
Baculovirus-Infected
Insect Cells
............... 295
Luke
O Shaußhnessy
and Sean Doyle
17
Purification of Poly-Histidine-Tagged Proteins
........................... 311
Sinéad T.
Loughran
ană Dermot
Walls
18
Immobilized
Metal
Affinity Chromatography/Reversed-Phase Enrichment
of Phosphopeptides and Analysis by CID/ETD Tandem Mass Spectrometry
..... 337
Rosana
Navajas,
Alberto
Paradela,
and Juan Pablo Albar
19
Tag Removal by Site-Specific Cleavage of
Recombinant
Fusion Proteins
........ 349
Adam Charlton and Michael Zachariou
20
Purification of Antibodies Using Affinity Chromatography
.................. 369
Elaine
Dar
су,
Paul Leonard, Jenny Fitzgerald, Martin Danaher,
and Richard O Kennedy
21
Optimized Generation of High-Affinity, High-Specificity
Single-Chain Fv Antibodies from Multiantigen Immunized Chickens
.......... 383
William
J
J. Finlay, Laird Bloom, and
Orla
Cunningham
22
Measuring Protein-Protein Interactions Using Biacore
..................... 403
Paul Leonard, Stephen Hearty, and Richard O Kennedy
23
Ultra-Performance Liquid Chromatography-Mass Spectrometry of Proteins
..... 419
Timothy R. Croley
24 Hydrophobie
Interaction Chromatography
............................. 431
Philip M. Cummins and Brendan F. O Connor
25
Fast Protein Liquid Chromatography
.................................. 439
Ashkan Madadlou, Siobhan O Sullivan, and David Sheehan
26
Shotgun Proteomics: A Qualitative Approach Applying Isoelectric
Focusing on Immobilized
pH
Gradient and LC-MS/MS
................... 449
Laurent
Geiser, Ali
R.
Vaezzadeh, Jacques M.P.
Deshusses,
and Denis F. Hochstrasser
27
Shotgun Proteomics: A Relative Quantitative Approach
Using Off-Gel Electrophoresis and LC-MS/MS
.......................... 459
Laurent
Geiser,
Loie
Dayon,
Ali
R.
Vaezzadeh, and Denis F. Hochstrasser
28
Clinical Proteomics: Liquid Chromatography-Mass Spectrometry
Purification Systems
............................................... 473
Michael Henry and Paula Meleady
29
Strategies for the Purification of Membrane Proteins
...................... 485
Sinead Marian Smith
30
A Multi-Step
Chromatographie
Strategy to Purify Three Fungal
Endo-ß-Glucanases................................................ 497
Tracey McCarthy and Maria G. Tuohy
Index
................................................................... 525
|
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| genre | (DE-588)4143413-4 Aufsatzsammlung gnd-content |
| genre_facet | Aufsatzsammlung |
| id | DE-604.BV036581372 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T22:43:23Z |
| institution | BVB |
| isbn | 9781607619123 9781607619130 |
| language | Undetermined |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-020502290 |
| oclc_num | 699630216 |
| open_access_boolean | |
| owner | DE-20 DE-11 DE-355 DE-BY-UBR DE-29T |
| owner_facet | DE-20 DE-11 DE-355 DE-BY-UBR DE-29T |
| physical | X, 527 S. Ill., graph. Darst. |
| publishDate | 2011 |
| publishDateSearch | 2011 |
| publishDateSort | 2011 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer Protocols |
| spelling | Protein chromatography ed. by Dermot Walls ... New York [u.a.] Humana Press 2011 X, 527 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 681 Springer Protocols Chromatographie (DE-588)4010153-8 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Proteine (DE-588)4076388-2 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Proteine (DE-588)4076388-2 s Chromatographie (DE-588)4010153-8 s Methode (DE-588)4038971-6 s DE-604 Walls, Dermot edt Methods in molecular biology 681 (DE-604)BV035362695 681 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020502290&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Protein chromatography Methods in molecular biology Chromatographie (DE-588)4010153-8 gnd Methode (DE-588)4038971-6 gnd Proteine (DE-588)4076388-2 gnd |
| subject_GND | (DE-588)4010153-8 (DE-588)4038971-6 (DE-588)4076388-2 (DE-588)4143413-4 |
| title | Protein chromatography |
| title_auth | Protein chromatography |
| title_exact_search | Protein chromatography |
| title_full | Protein chromatography ed. by Dermot Walls ... |
| title_fullStr | Protein chromatography ed. by Dermot Walls ... |
| title_full_unstemmed | Protein chromatography ed. by Dermot Walls ... |
| title_short | Protein chromatography |
| title_sort | protein chromatography |
| topic | Chromatographie (DE-588)4010153-8 gnd Methode (DE-588)4038971-6 gnd Proteine (DE-588)4076388-2 gnd |
| topic_facet | Chromatographie Methode Proteine Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020502290&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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