Thiol redox transitions in cell signaling: A Chemistry and biochemistry of low molecular weight and protein thiols
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Amsterdam [u.a.]
Elsevier, AP
2010
|
| Ausgabe: | 1. ed. |
| Schriftenreihe: | Methods in enzymology
473 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XLVIII, 359 S. Ill., graph. Darst. |
| ISBN: | 9780123813459 |
Internformat
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Datensatz im Suchindex
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|---|---|
| adam_text | Contents
Contributors
xi
Preface
xix
Volumes in Series
xxi
1.
Changing Paradigms in Thiology: From
Antioxidant
Defense
Toward
Redox
Regulation
1
Leopold
Flohé
1.
Introduction
2
2.
Early Concepts, Misconceptions, Unsettled Battles, and
Persistent Confusion
3
3.
The Discovery of Natural Free Radicals
6
4.
Love Affairs Between
Hydroperoxides
and Thiolates or
Selenolates
9
5.
Toward Regulatory Circuits with Puzzle Stones from
Redox
Biochemistry
19
6.
Conclusions and Perspectives
27
References
28
2.
Mass Spectrometry-Based Methods for the Determination
of Sulfur and Related Metabolite Concentrations in Cell Extracts
41
Emmanuel Godat, Geoffrey Madalinski,
Ludovic
Muller,
Jean-François
Heilier,
Jean Labarre, and Christophe Junot
1.
Introduction
42
2.
Analytical Methods for the Determination of Sulfur and
Amino
Acid Metabolites
44
3.
Procedures
46
4.
Absolute LC-ESI-MS/MS Quantification of Thiol and
Amino
Acid
Metabolites in Yeast Extracts
49
5.
Qualitative and Quantitative Determination of Thiol and
Amino
Acid Metabolites in Yeast Extracts by Using an LTQ-Orbitrap
Mass Spectrometer
64
6.
Discussion
68
7.
Summary
70
Acknowledgments
73
References
73
vi
Contents
3.
Use
of
Dimedone-Based Chemical
Probes for Sulfenic Acid Detection:
Evaluation of Conditions Affecting Probe Incorporation into
Redox-Sensitive
Proteins
77
Chananat Klomsiri, Kimberly J. Nelson,
Erika Bechtold,
Laura Soito,
Lynnette
С
Johnson, W. Todd Lowther, Seong-Eon Ryu, S. Bruce King,
Cristina
M.
Furdui,
and Leslie
В.
Poole
1.
introduction
78
2.
Materials
81
3.
Methods
83
4.
Summary
92
Acknowledgments
92
References
93
4.
Use of Dimedone-Based Chemical Probes for Sulfenic Acid
Detection: Methods to Visualize and Identify Labeled Proteins
95
Kimberly j. Nelson, Chananat Klomsiri,
Simona
G. Codreanu,
Laura Soito,
Daniel
С
Liebler,
LeAnn
С.
Rogers, Larry W. Daniel, and Leslie B. Poole
1.
Introduction
96
2.
Biotin-Based Affinity Capture to Identify Proteins Containing
Cysteine
Sulfenic Acids
98
3.
Detection Methods to Identify Oxidized Proteins and Cysteines
103
4.
Summary
112
Acknowledgments
113
References
113
5.
Formation and Reactions of Sulfenic Acid in Human
Serum Albumin
117
Beatriz
Alvarez,
Sebastián
Carballal,
Lucía
Turell, and Rafael
Radi
1.
Introduction
118
2.
Preparation of Albumin Solutions
119
3.
Preparation of Oxidized Albumin
123
4.
Detection of Albumin Sulfenic Acid
125
5.
Quantification of Albumin Sulfenic Acid Using
Thionitrobenzoate (TNB)
131
6.
Reactivity of Sulfenic Acid
132
7.
Detection of Albumin Sulfinic Acid
134
8.
Conclusions
134
Acknowledgments
134
References
135
Contents VÜ
6.
Determination of GSH, GSSG, and GSNO Using HPLC with
Electrochemical Detection
137
Li-Peng Yap, Harsh Sancheti, Maria D. Ybanez, Jerome Garcia,
Enrique
Cadenas,
and Derick Han
1.
Introduction
138
2.
Methods
139
3.
Summary
145
Acknowledgments
146
References
146
7.
Measurement of Mixed Disulfides Including Glutathionylated
Proteins
149
Raffaella
Priora,
Lucia
Coppo,
Sonia
Salzano,
Paolo
Di Simplicio,
and
Pietro Ghezzi
1.
Introduction
150
2.
Chemical
Quantification of
PSSX, PSSG, and PSSC 151
3.
Visualization of PSSG by
Western
Blot
156
Acknowledgments
157
References
158
8.
Detection and Quantiflcation of Protein Disulfides in Biological
Tissues: A Fluorescence-Based Proteomic Approach
161
Viviana I. Pérez, Anson
Pierce,
Eric M. de
Waal, Walter F. Ward,
Alex
Bokov,
Asish Chaudhuri, and
Arlan
Richardson
1.
Introduction
163
2.
Material and Methods
164
3.
Results
169
4.
Discussion
174
Acknowledgments
175
References
175
9.
Measurement and fdentiflcation of 5-Glutathiolated Proteins
179
Bradford
G. Hill, Kota
V.
Ramana,
jian
Cai, Aruni Bhatnagar,
and Satish K. Srivastava
1.
Introduction
180
2.
Chemical
Methods for the Measurement of Glutathiolated Proteins
182
3.
Detection of Glutathiolated Proteins by ESI/MS
184
4.
Identification of S-Glutathiolated Proteins in Cells and Tissues
186
5.
Conclusions
194
Acknowledgment
196
References
196
v¡¡¡
Contents
10.
Proteome
Screens for Cys Residues Oxidation: The Redoxome
199
Giovanni Chiappetta, Sega Ndiaye, Aeid Igbaria, Chitranshu Kumar,
Joetle Vinh, and Michel B.
Toledano
1.
Introduction
200
2.
General Considerations
201
3.
Overview of the Different Methods
202
4.
Results and Discussion
206
5.
Conclusions
214
Acknowledgments
214
References
214
11.
Identification by MS/MS of Disulfides Produced by a Functional
Redox
Transition
217
Pierluigi Mauri,
Stefano
Toppo,
Antonella De Palma, Louise Benazzi,
Matilde
Maiorino, and Fulvio
Ursini
1.
Introduction
218
2. MS/MS Identification
of
Redox-Switches in Protein 219
3.
Analytical Procedure
220
4.
Discussion
222
References
224
12.
Mass Spectrometry Approaches for the
Redox
Characterization
of Protein
Cysteine
Residues: The Case of the Transcription
Factor Pax-8
227
Andrea
Scaloni
and Gianluca Tell
1.
Introduction
228
2.
Materials and Methods
230
3.
Results
232
4.
Conclusions and Future Perspectives
241
Acknowledgments
243
References
243
13.
A Simple Method to Systematically Study Oxidatively Modified
Proteins in Biological Samples and Its Applications
251
Byoung-Joon Song, Soo-Kyung
Suh,
and Kwan-Hoon Moon
1.
introduction
252
2.
Materials
254
3.
Methods
255
4.
Discussion
257
Acknowledgment
262
References
262
Contents ix
14.
Direct
and Indirect Detection Methods for the Analysis
of 5-Nitrosylated Peptides and Proteins
265
Federico
Torta, Lisa
Elviri,
and Angela
Bachi
1.
Introduction
266
2.
Indirect Detection of S-Nitrosylated Proteins: His-tag Switch
268
3.
Direct Analysis of S-Nitrosylation by MS
274
4.
Conclusions
278
References
278
15.
A Rapid Approach for the Detection, Quantification, and Discovery
of Novel Sulfenic Acid or 5-Nitrosothiol Modified Proteins
Using a Biotin-Switch Method
281
Joseph R. Burgoyne and Philip Eaton
1.
Introduction
282
2.
Overview of Analytical Strategy
292
3.
Conclusions
296
Acknowledgments
297
References
297
16.
Protein Adducts of Aldehydic
Lipid
Peroxidation
Products:
Identification and Characterization of Protein Adducts Using
an Aldehyde/Keto-Reactive Probe in Combination with
Mass Spectrometry
305
Claudia S.
Maier, Juan
Chavez, Jing Wang, and Jianyong Wu
1.
Introduction
306
2.
Modification of Proteins by Aldehydic
Lipid
Peroxidation
Products
307
3.
Redox
Proteomics of Protein Targets of Reactive
Lipid
Peroxidation
Products
308
4.
Mass Spectrometry-Based Approaches for the Identification
and Characterization of Protein Adducts of Aldehydic
Lipid
Peroxidation
Products
310
5.
Experimental Strategy of Using an Aldehyde/Keto-Reactive Probe
for the Targeted Analysis of Protein Adducts of Aldehydic
Lipid
Peroxidation
Products
311
6.
Applications of the ARP-Labeling Strategy
316
7.
Interpretation of MS/MS Spectra of Protein Adducts and the
Use of Diagnostic Marker Ions
323
8.
Conclusion
324
Acknowledgment
327
References
328
Author Index
331
Subject Index
353
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| spelling | Thiol redox transitions in cell signaling A Chemistry and biochemistry of low molecular weight and protein thiols ed. by Enrique Cadenas ... 1. ed. Amsterdam [u.a.] Elsevier, AP 2010 XLVIII, 359 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in enzymology 473 Methods in enzymology ... Labortechnik (DE-588)4123602-6 gnd rswk-swf Thiole (DE-588)4185267-9 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Thiole (DE-588)4185267-9 s Labortechnik (DE-588)4123602-6 s b DE-604 Cadenas, Enrique Sonstige oth (DE-604)BV036555262 1 Methods in enzymology 473 (DE-604)BV000000938 473 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020476748&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Thiol redox transitions in cell signaling Methods in enzymology Labortechnik (DE-588)4123602-6 gnd Thiole (DE-588)4185267-9 gnd |
| subject_GND | (DE-588)4123602-6 (DE-588)4185267-9 (DE-588)4143413-4 |
| title | Thiol redox transitions in cell signaling |
| title_auth | Thiol redox transitions in cell signaling |
| title_exact_search | Thiol redox transitions in cell signaling |
| title_full | Thiol redox transitions in cell signaling A Chemistry and biochemistry of low molecular weight and protein thiols ed. by Enrique Cadenas ... |
| title_fullStr | Thiol redox transitions in cell signaling A Chemistry and biochemistry of low molecular weight and protein thiols ed. by Enrique Cadenas ... |
| title_full_unstemmed | Thiol redox transitions in cell signaling A Chemistry and biochemistry of low molecular weight and protein thiols ed. by Enrique Cadenas ... |
| title_short | Thiol redox transitions in cell signaling |
| title_sort | thiol redox transitions in cell signaling chemistry and biochemistry of low molecular weight and protein thiols |
| topic | Labortechnik (DE-588)4123602-6 gnd Thiole (DE-588)4185267-9 gnd |
| topic_facet | Labortechnik Thiole Aufsatzsammlung |
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