Functional analysis of the insulin-like growth factor binding protein-related protein Mig30 in Xenopus laevis:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2010
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | IV, 137 S. Ill., graph. Darst. 21 cm |
Internformat
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV036496937 | ||
| 003 | DE-604 | ||
| 005 | 20161104 | ||
| 007 | t| | ||
| 008 | 100610s2010 xx ad|| m||| 00||| eng d | ||
| 015 | |a 10,H06 |2 dnb | ||
| 016 | 7 | |a 1000165809 |2 DE-101 | |
| 035 | |a (OCoLC)705584330 | ||
| 035 | |a (DE-599)BVBBV036496937 | ||
| 040 | |a DE-604 |b ger |e rakddb | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-12 |a DE-29T |a DE-188 | ||
| 082 | 0 | |a 612.01 |2 22/ger | |
| 084 | |a 610 |2 sdnb | ||
| 100 | 1 | |a Kürner, Klaus-Michael |d 1973- |e Verfasser |0 (DE-588)140362789 |4 aut | |
| 245 | 1 | 0 | |a Functional analysis of the insulin-like growth factor binding protein-related protein Mig30 in Xenopus laevis |c von Klaus-Michael Kürner |
| 264 | 1 | |c 2010 | |
| 300 | |a IV, 137 S. |b Ill., graph. Darst. |c 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Tübingen, Univ., Diss., 2009 | ||
| 650 | 0 | 7 | |a Insulin-like-Growth-Factor-Binding-Proteins |0 (DE-588)4646051-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Glatter Krallenfrosch |0 (DE-588)4157477-1 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Glatter Krallenfrosch |0 (DE-588)4157477-1 |D s |
| 689 | 0 | 1 | |a Insulin-like-Growth-Factor-Binding-Proteins |0 (DE-588)4646051-2 |D s |
| 689 | 0 | |5 DE-604 | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020419440&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-020419440 | |
Datensatz im Suchindex
| _version_ | 1817311101799366656 |
|---|---|
| adam_text |
TABLE
OF
CONTENTS
ZUSAMMENFASSUNG
1
ABSTRACT
II
TABLE
OF
CONTENTS
III
1
INTRODUCTION
1
1.1
OVERVIEW
OF
THE
IGF
SYSTEM
2
1.2
IGF-BINDING
PROTEINS
6
1.3
IGFBP-RELATED
PROTEINS
8
1.4
IGF
SIGNALLING
IN
XENOPUS
LAEVIS
11
1.5
SIGNIFICANCE
AND
AIMS
OF
THIS
WORK
12
2
RESULTS
14
2.1
IDENTIFICATION
AND
CLASSIFICATION
OF
A
NEW
SUBGROUP
OF
IGFBP-RPS
14
2.2
CLONING
AND
COMPARATIVE
EXPRESSION
ANALYSIS
OF
THREE
IGFBP-RPS
17
EXPRESSION
ANALYSIS
OF
MIG30
19
EXPRESSION
ANALYSIS
OF
XIGFBP-RP10
19
EXPRESSION
ANALYSIS
OF
XIGFBP-LIKE
20
2.3
MIG30
IS
A
SECRETED
PROTEIN
OF
THE
SPEMANN-MANGOLD
ORGANISER
23
2.4
OVEREXPRESSION
OF
MIG30
INDUCES
ECTOPIC
PATCHES
OF
CEMENT
GLAND
TISSUE
28
2.5
EXPRESSION
OF
MIG30
MUTANT
CONSTRUCTS
REVEALS
DISCRETE
ACTIVITIES
30
2.6
OVEREXPRESSION
OF
MIG30
CAN
PHENOCOPY
GAIN
OF
IGF
SIGNALS
36
2.7
OVEREXPRESSION
OF
MIG30
INHIBITS
CANONICAL
WNT
SIGNALLING
40
2.8
OVEREXPRESSION
OF
MIG30
CAN
COOPERATE
WITH
IGF
SIGNALS
41
2.9
OVEREXPRESSION
OF
MIG30
INHIBITS
CELL
SPREADING
BEHAVIOUR
43
2.10
OVEREXPRESSION
OF
MIG30
INHIBITS
CERBERUS
ACTIVITIES
44
2.11
FURTHER
CHARACTERISATION
OF
MIG30
MUTANT
CONSTRUCTS
46
2.12
MIG30
IS
REQUIRED
FOR
NEURAL
PLATE
AND
NEURAL
CREST
DEVELOPMENT
51
2.13
KNOCKDOWN
OF
MIG30
PERTURBES
FORMATION
OF
BRACHET
'
S
CLEFT
57
2.14
KNOCKDOWN
OF
MIG30
DOES
NOT
ALTER
LEVELS
OF
ACTIVATED
ERK1/2
IN
EARLY
GASTRULA
EMBRYOS
58
2.15
PRECISE
SPATIO-TEMPORAL
EXPRESSION
OF
MIG30
IS
REQUIRED
FOR
NORMAL
DEVELOPMENT
59
3
DISCUSSION
66
3.1
MIG30
INTERACTS
WITH
THE
IGF
PATHWAY
66
3.2
MIG30
IS
REQUIRED
FOR
NEURAL
PLATE
AND
NEURAL
CREST
DEVELOPMENT
70
3.3
MIG30
FUNCTION
IS
REQUIRED
FOR
XBRA
TRANSCRIPTION
75
3.4
FURTHER
IGF-INDCPENDENT
FUNCTIONS
OF
MIG30
76
3.5
MIG30
ALTERS
MORPHOGENETIC
CELL
BEHAVIOURS
78
3.6
SUMMARY
AND
FUTURE
PERSPECTIVES
83
4
MATERIALS
AND
METHODS
86
4.1
SEQUENCE
DATABASES
AND
TOOLS
86
4.2
EQUIPMENT
AND
MATERIALS
86
4.3
REAGENTS
87
4.4
ANIMALS,
BACTERIAL
HOST
STRAINS,
AND
CELL
LINES
88
4.5
BASIC
MOLECULAR
BIOLOGY
TECHNIQUES
89
4.6
CLONING
OF
DNA
CONSTRUCTS
USED
IN
THIS
WORK
93
4.7
REVERSE
TRANSCRIPTION
POLYMERASE
CHAIN
REACTION
(RT-PCR)
98
4.8
IN
VITRO
TRANSCRIPTION
100
4.9
METHODS
USING
XENOPUS
LAEVIS
OOCYTES
AND
EMBRYOS
101
4.10
EXPLANT
AND
CELL
SPREADING
ASSAYS
103
4.11
IN
SITU
HYBRIDISATION
104
4.12
IMMUNOHISTOCHEMISTRY
106
4.13
PROTEIN
CHEMISTRY
107
4.14
LUCIFERASE
REPORTER
ASSAY
109
4.15
CELL
CULTURE
TECHNIQUES
110
5
REFERENCES
115
6
TABLE
OF
FIGURES
132
7
TABLE
OF
TABLES
133
8
ABBREVIATIONS
133 |
| any_adam_object | 1 |
| author | Kürner, Klaus-Michael 1973- |
| author_GND | (DE-588)140362789 |
| author_facet | Kürner, Klaus-Michael 1973- |
| author_role | aut |
| author_sort | Kürner, Klaus-Michael 1973- |
| author_variant | k m k kmk |
| building | Verbundindex |
| bvnumber | BV036496937 |
| ctrlnum | (OCoLC)705584330 (DE-599)BVBBV036496937 |
| dewey-full | 612.01 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 612 - Human physiology |
| dewey-raw | 612.01 |
| dewey-search | 612.01 |
| dewey-sort | 3612.01 |
| dewey-tens | 610 - Medicine and health |
| discipline | Medizin |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>00000nam a2200000 c 4500</leader><controlfield tag="001">BV036496937</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20161104</controlfield><controlfield tag="007">t|</controlfield><controlfield tag="008">100610s2010 xx ad|| m||| 00||| eng d</controlfield><datafield tag="015" ind1=" " ind2=" "><subfield code="a">10,H06</subfield><subfield code="2">dnb</subfield></datafield><datafield tag="016" ind1="7" ind2=" "><subfield code="a">1000165809</subfield><subfield code="2">DE-101</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)705584330</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV036496937</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakddb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-12</subfield><subfield code="a">DE-29T</subfield><subfield code="a">DE-188</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">612.01</subfield><subfield code="2">22/ger</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">610</subfield><subfield code="2">sdnb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Kürner, Klaus-Michael</subfield><subfield code="d">1973-</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)140362789</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Functional analysis of the insulin-like growth factor binding protein-related protein Mig30 in Xenopus laevis</subfield><subfield code="c">von Klaus-Michael Kürner</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2010</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">IV, 137 S.</subfield><subfield code="b">Ill., graph. Darst.</subfield><subfield code="c">21 cm</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="a">Tübingen, Univ., Diss., 2009</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Insulin-like-Growth-Factor-Binding-Proteins</subfield><subfield code="0">(DE-588)4646051-2</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Glatter Krallenfrosch</subfield><subfield code="0">(DE-588)4157477-1</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Glatter Krallenfrosch</subfield><subfield code="0">(DE-588)4157477-1</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Insulin-like-Growth-Factor-Binding-Proteins</subfield><subfield code="0">(DE-588)4646051-2</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020419440&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="943" ind1="1" ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-020419440</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV036496937 |
| illustrated | Illustrated |
| indexdate | 2024-12-02T07:02:00Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-020419440 |
| oclc_num | 705584330 |
| open_access_boolean | |
| owner | DE-12 DE-29T DE-188 |
| owner_facet | DE-12 DE-29T DE-188 |
| physical | IV, 137 S. Ill., graph. Darst. 21 cm |
| publishDate | 2010 |
| publishDateSearch | 2010 |
| publishDateSort | 2010 |
| record_format | marc |
| spelling | Kürner, Klaus-Michael 1973- Verfasser (DE-588)140362789 aut Functional analysis of the insulin-like growth factor binding protein-related protein Mig30 in Xenopus laevis von Klaus-Michael Kürner 2010 IV, 137 S. Ill., graph. Darst. 21 cm txt rdacontent n rdamedia nc rdacarrier Tübingen, Univ., Diss., 2009 Insulin-like-Growth-Factor-Binding-Proteins (DE-588)4646051-2 gnd rswk-swf Glatter Krallenfrosch (DE-588)4157477-1 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Glatter Krallenfrosch (DE-588)4157477-1 s Insulin-like-Growth-Factor-Binding-Proteins (DE-588)4646051-2 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020419440&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Kürner, Klaus-Michael 1973- Functional analysis of the insulin-like growth factor binding protein-related protein Mig30 in Xenopus laevis Insulin-like-Growth-Factor-Binding-Proteins (DE-588)4646051-2 gnd Glatter Krallenfrosch (DE-588)4157477-1 gnd |
| subject_GND | (DE-588)4646051-2 (DE-588)4157477-1 (DE-588)4113937-9 |
| title | Functional analysis of the insulin-like growth factor binding protein-related protein Mig30 in Xenopus laevis |
| title_auth | Functional analysis of the insulin-like growth factor binding protein-related protein Mig30 in Xenopus laevis |
| title_exact_search | Functional analysis of the insulin-like growth factor binding protein-related protein Mig30 in Xenopus laevis |
| title_full | Functional analysis of the insulin-like growth factor binding protein-related protein Mig30 in Xenopus laevis von Klaus-Michael Kürner |
| title_fullStr | Functional analysis of the insulin-like growth factor binding protein-related protein Mig30 in Xenopus laevis von Klaus-Michael Kürner |
| title_full_unstemmed | Functional analysis of the insulin-like growth factor binding protein-related protein Mig30 in Xenopus laevis von Klaus-Michael Kürner |
| title_short | Functional analysis of the insulin-like growth factor binding protein-related protein Mig30 in Xenopus laevis |
| title_sort | functional analysis of the insulin like growth factor binding protein related protein mig30 in xenopus laevis |
| topic | Insulin-like-Growth-Factor-Binding-Proteins (DE-588)4646051-2 gnd Glatter Krallenfrosch (DE-588)4157477-1 gnd |
| topic_facet | Insulin-like-Growth-Factor-Binding-Proteins Glatter Krallenfrosch Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020419440&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT kurnerklausmichael functionalanalysisoftheinsulinlikegrowthfactorbindingproteinrelatedproteinmig30inxenopuslaevis |