Practical cell analysis:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Hoboken, N.J.
Wiley
2010
|
| Ausgabe: | 1. pupl. |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Includes bibliographical references and index |
| Beschreibung: | XX, 294 S. Ill., graph. Darst. |
| ISBN: | 9780470741559 |
Internformat
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Datensatz im Suchindex
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|---|---|
| adam_text | PRACTICAL CELL ANALYSIS DIMITRI PAPPAS DEPT OF CHEMISTRY & BIOCHEMISTRY,
TEXAS TECH UNIVERSITY, USA WILEY A JOHN WILEY AND SONS, LTD, PUBLICATION
CONTENTS PREFACE XIII ACKNOWLEDGMENTS XIX 1 GETTING STARTED (AND GETTING
THE CELLS) 1 1.1 INTRODUCTION 1 1.2 THE DRIVING NEED 2 1.3 PRIMARY AND
CULTURED CELLS 3 1.4 CHOOSING A CULTURED CELL 6 1.5 CHOOSING PRIMARY
CELLS 11 1.6 EASILY OBTAINABLE PRIMARY CELLS 14 1.7 PRIMARY CELLS FROM
TISSUES 16 1.8 PURIFYING PRIMARY CELLS 21 1.9 HOW LONG DO PRIMARY CELLS
REMAIN PRIMARY? 24 1.10 OBTAINING PRIMARY CELLS FROM A COMMERCIAL SOURCE
25 1.11 BACTERIA AND YEAST 26 1.12 PRACTICAL ASPECTS OF CELL CULTURE 27
1.13 SAFETY ASPECTS OF PRIMARY AND TRANSFORMED CELL LINES 29 1.14
TRANSFECTION OF PRIMARY AND TRANSFORMED CELL LINES 30 1.15 CONCLUSION 32
REFERENCES 32 2 THE CELL-CULTURE LABORATORY (TOOLS OF THE TRADE) 35 2.1
INTRODUCTION 35 2.2 ISSUES CONCERNING A CELL LABORATORY 36 2.3 SETTING
UP A CELL CULTURE LABORATORY 44 2.4 CELL LINE STORAGE 48 VIII CONTENTS
2.5 2.6 2.7 2.8 2.9 2.10 PERSONAL PROTECTIVE EQUIPMENT CELL AND SAMPLE
HANDLING COMMON ANALYTICAL INSTRUMENTATION FOR CELL CULTURE
CONSIDERATIONS WHEN SETTING UP A CELL-CULTURE LABORATORY ESTABLISHING
AND REGULATING A CULTURE FACILITY CONCLUSION REFERENCES 3 MAINTAINING
CULTURES 3.1 3.2 3.3 3.4 3.5 3.6 3.7 3.8 INTRODUCTION MEDIUM THE USE OF
MEDIUM IN ANALYSIS, AND ALTERNATIVES CULTURING CELLS PROTOCOL 3.1:
SUB-CULTURE OF ADHERENT CELLS GROWING CELLS IN THREE DIMENSIONS
STERILITY AND CONTAMINATION OF CULTURE STORAGE OF CELL SAMPLES AND CELL
LINES PROTOCOL 3.2: CRYOPRESERVATION OF MAMMALIAN CELLS PROTOCOL 3.3:
RETRIEVAL OF CELLS FROM LIQUID-NITROGEN STORAGE CONCLUSION REFERENCES 4
MICROSCOPY OF CELLS 4.1 4.2 4.3 4.4 4.5 4.6 4.7 4.8 4.9 4.10 4.11
INTRODUCTION MICROSCOPE TYPES CULTURING CELLS FOR MICROSCOPY SIGNALS,
BACKGROUND, AND ARTIFACTS IN OPTICAL MICROSCOPY STAINING CELLS FOR
FLUORESCENCE MICROSCOPY PROTOCOL 4.1 FIXATION OF CELLS FOR
IMMUNOCHEMICAL STAINING MULTIPLE LABELS VIABILITY AND TWO-PHOTON
MICROSCOPY SPATIAL RESOLUTION IN OPTICAL MICROSCOPY IMAGE SATURATION AND
INTENSITY ATOMIC FORCE AND ENVIRONMENTAL SCANNING ELECTRON MICROSCOPY
CONCLUSION REFERENCES 51 52 53 57 61 63 63 65 65 66 71 73 75 77 79 80 83
84 86 86 89 89 90 95 101 104 111 113 116 117 119 120 121 122 CONTENTS IX
5 SEPARATING CELLS 125 5.1 INTRODUCTION 125 5.2 THE CELL SAMPLE 126 5.3
LABEL-FREE (INTRINSIC) SEPARATIONS 131 5.4 IMMUNOMAGNETIC SORTING 134
5.5 CELL-AFFINITY CHROMATOGRAPHY 137 5.6 AFFINITY CHEMISTRY
CONSIDERATIONS IN CAC AND MACS SEPARATIONS 142 PROTOCOL 5.1 ^SCREENING
OF ANTIBODY CLONES 145 5.7 ELUTION IN CELL-AFFINITY CHROMATOGRAPHY 147
5.8 NONSPECIFIC BINDING IN CELL SEPARATIONS 148 5.9 SEPARATION OF RARE
CELLS 151 5.10 FLUORESCENCE-ACTIVATED CELL SORTING 152 5.11 SORTING
PARAMETERS 156 5.12 OTHER SEPARATION TECHNIQUES AND CONSIDERATIONS 156
5.13 CONCLUSION 160 REFERENCES 161 6 FLOW CYTOMETRY: CELL ANALYSIS IN
THE FAST LANE 165 6.1 INTRODUCTION 165 6.2 THE CELL SAMPLE 167 6.3 FLOW
CYTOMETER FUNCTION 169 6.4 OBTAINING OR FINDING A FLOW CYTOMETER 177 6.5
USING FLOW CYTOMETERS 178 6.6 SETTING UP A FLOW CYTOMETER FOR
MULTI-COLOR STAINING 181 6.7 ANALYZING FLOW CYTOMETRY DATA 185 6.8
EXAMPLE FLOW-CYTOMETRY ASSAYS 189 6.9 NO-FLOW CYTOMETRY 191 6.10
CONCLUSION 192 REFERENCES 192 7 ANALYZING CELLS WITH MICROFLUIDIC
DEVICES 195 7.1 INTRODUCTION 195 7.2 ADVANTAGES OF MICROFLUIDICS 196 7.3
CONSIDERATIONS OF MICROFLUIDICS AND CELLS 198 7.4 OBTAINING MICROFLUIDIC
CELL DEVICES 202 7.5 MICROFLUIDIC FLOW CYTOMETRY 209 7.6 CELL
SEPARATIONS 213 7.7 ANALYSIS OF CELL PRODUCTS 215 7.8 CELL CULTURE 219
PROTOCOL 7.1: LOW-SHEAR CELL-CULTURE CHIP 222 CONTENTS * 7.9 CONCLUSION
225 REFERENCES 225 STATISTICAL CONSIDERATIONS 229 8.1 INTRODUCTION 229
8.2 TYPES OF ERROR 230 8.3 FIGURES OF MERIT IN STATISTICAL ANALYSIS OF
CELLS 236 8.4 LIMITS OF DETECTION AND QUANTITATION (OF CELL) 240 8.5
METHODS TO IMPROVE CELL STATISTICS 242 8.6 COMPARING ANALYTICAL VALUES
243 8.7 REJECTING DATA: PROCEED WITH CAUTION 245 8.8 CONCLUSION 245
REFERENCES 246 PROTOCOLS, PROBES, AND STANDARDS 247 9.1 INTRODUCTION 247
9.2 CELL TRANSFECTION AND IMMORTALIZATION (CHAPTER 1) 247 PROTOCOL 9.1:
TRANSFECTING CELLS WITH POLYAMINE REAGENTS 248 PROTOCOL 9.2: STABLE
TRANSFECTION USING POLYAMINE DELIVERY 249 PROTOCOL 9.3: TRANSFECTION
USING ELECTROPORATION 251 PROTOCOL 9.4: CELL IMMORTALIZATION USING HTERT
TRANSFECTION 255 9.3 CALCULATING RELATIVE CENTRIFUGAL FORCE (RCF) AND
CENTRIFUGE ROTOR SPEED (CHAPTER 2) 256 9.4 FLUORESCENCE METHODS
(CHAPTERS 4 AND 6) 256 PROTOCOL 9.4: APOPTOSIS DETECTION USING
FLUOROPHORE-CONJUGATED ANNEXIN-V AND A VIABILITY DYE 257 PROTOCOL 9.5:
APOPTOSIS DETECTION USING FLUOROGENIC CASPASE PROBES 263 9.5 SURFACE
MODIFICATIONS FOR CELL ANALYSIS (CHAPTERS 5 AND 7) 266 PROTOCOL 9.6:
COVALENT LINKAGE OF PROTEINS (NONANTIBODY) TO GLASS BY MICROCONTACT
IMPRINTING 266 PROTOCOL 9.7: COVALENT LINKAGE OF ANTIBODIES TO GLASS 269
PROTOCOL 9.8: NONCOVALENT ATTACHMENT OF ANTIBODIES TO GLASS #1 271
PROTOCOL 9.9: NONCOVALENT ATTACHMENT OF ANTIBODIES TO GLASS OR PDMS #2
272 PROTOCOL 9.10: BLOCKING ENDOGENOUS BIOTIN 273 CONTENTS XI 9.6 FLOW
CYTOMETRY AND CELL SEPARATIONS (CHAPTERS 5 AND 6) 274 PROTOCOL 9.11:
CELL CYCLE MEASUREMENTS BY FLOW CYTOMETRY 274 PROTOCOL 9.12: ANTIGEN
DENSITY MEASUREMENTS IN FLOW CYTOMETRY 275 PROTOCOL 9.13: ANTIGEN
DENSITY MEASUREMENTS USING FLUORESCENCE CORRELATION SPECTROSCOPY 279
PROTOCOL 9.14;,CELL PROLIFERATION USING ANTI-CD71 STAINING (CHAPTERS 4
AND 6) 281 9.7 FLUORESCENT LABELS AND FLUOROGENIC PROBES (CHAPTERS 4-7)
283 REFERENCES 284 INDEX 287
|
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| discipline | Biologie |
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| isbn | 9780470741559 |
| language | English |
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| spelling | Pappas, Dimitri Verfasser aut Practical cell analysis Dimitri Pappas 1. pupl. Hoboken, N.J. Wiley 2010 XX, 294 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Includes bibliographical references and index Cytology Technique Cytologie (DE-588)4070177-3 gnd rswk-swf Labortechnik (DE-588)4123602-6 gnd rswk-swf Cytologie (DE-588)4070177-3 s Labortechnik (DE-588)4123602-6 s DE-604 HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020327516&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Pappas, Dimitri Practical cell analysis Cytology Technique Cytologie (DE-588)4070177-3 gnd Labortechnik (DE-588)4123602-6 gnd |
| subject_GND | (DE-588)4070177-3 (DE-588)4123602-6 |
| title | Practical cell analysis |
| title_auth | Practical cell analysis |
| title_exact_search | Practical cell analysis |
| title_full | Practical cell analysis Dimitri Pappas |
| title_fullStr | Practical cell analysis Dimitri Pappas |
| title_full_unstemmed | Practical cell analysis Dimitri Pappas |
| title_short | Practical cell analysis |
| title_sort | practical cell analysis |
| topic | Cytology Technique Cytologie (DE-588)4070177-3 gnd Labortechnik (DE-588)4123602-6 gnd |
| topic_facet | Cytology Technique Cytologie Labortechnik |
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