Practical high-performance liquid chromatography:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English German |
| Veröffentlicht: |
Chichester [u.a.]
Wiley
2010
|
| Ausgabe: | 5. ed. |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XIII, 412 S. Ill., graph. Darst. |
| ISBN: | 9780470682180 9780470682173 |
Internformat
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| 245 | 1 | 0 | |a Practical high-performance liquid chromatography |c Veronika R. Meyer |
| 246 | 1 | 3 | |a Practical high performance liquid chromatography |
| 250 | |a 5. ed. | ||
| 264 | 1 | |a Chichester [u.a.] |b Wiley |c 2010 | |
| 300 | |a XIII, 412 S. |b Ill., graph. Darst. | ||
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Datensatz im Suchindex
| _version_ | 1804142905750716416 |
|---|---|
| adam_text | Titel: Practical high-performance liquid chromatography
Autor: Meyer, Veronika R.
Jahr: 2010
Contents
Preface to the Fifth Edition..................... xiii
Important and Useful Equations for HPLC.............. 1
1 Introduction........................... 5
1.1 HPLC: A powerful separation method.......... 5
1.2 A first HPLC experiment................. 5
1.3 Liquid chromatographic separation modes....... 8
1.4 The HPLC instrument................... 9
1.5 Safety in the HPLC laboratory.............. 10
1.6 Comparison between high-performance liquid
chromatography and gas chromatography....... 11
1.7 Comparison between high-performance liquid
chromatography and capillary electrophoresis..... 12
1.8 Units for pressure, length and viscosity......... 13
1.9 Scientific journals.................... 14
1.10 Recommended books.................. 15
2 Theoretical Principles...................... 17
2.1 The chromatographic process.............. 17
2.2 Band broadening..................... 19
2.3 The chromatogram and its purport........... 23
2.4 Graphical representation of peak pairs with
different degree of resolution.............. 30
2.5 Factors affecting resolution............... 35
2.6 Extra-column volumes (dead volumes)......... 40
2.7 Tailing.......................... 41
2.8 Peak capacity and statistical resolution probability ... 46
2.9 Effects of temperature in HPLC............. 49
2.10 The limits of HPLC.................... 51
2.11 How to obtain peak capacity............... 55
vii
v¡¡¡ Contents
3 Pumps.............................. 59
3.1 General requirements.................. 59
3.2 The short-stroke piston pump.............. 59
3.3 Maintenance and repair................. 62
3.4 Other pump designs................... 63
4 Preparation of Equipment up to Sample Injection....... 65
4.1 Selection of the mobile phase.............. 65
4.2 Preparation of the mobile phase............. 67
4.3 Gradient systems..................... 68
4.4 Capillary tubing..................... 70
4.5 Fittings.......................... 72
4.6 Sample injectors..................... 74
4.7 Sample solution and sample volume.......... 78
5 Solvent Properties....................... 81
5.1 Table of organic solvents................ 81
5.2 Solvent selectivity.................... 83
5.3 Miscibility......................... 83
5.4 Buffers.......................... 84
5.5 Shelf life of mobile phases................ 87
5.6 The mixing cross..................... 88
6 Detectors............................ 91
6.1 General.......................... 91
6.2 UV detectors....................... 96
6.3 Refractive index detectors................ 99
6.4 Fluorescence detectors................. 101
6.5 Electrochemical (amperometric) detectors...... 103
6.6 Light-scattering detectors............... 104
6.7 Other detectors..................... 106
6.8 Multiple detection................... 107
6.9 Indirect detection.................... 108
6.10 Coupling with spectroscopy.............. 109
7 Columns and Stationary Phases............... 117
7.1 Columns for HPLC................... 117
7.2 Precolumns....................... 119
7.3 General properties of stationary phases........ 120
7.4 Silica.......................... 125
7.5 Chemically modified silica............... 126
7.6 Styrene-divinylbenzene................ 129
Contents ¡x
7.7 Some other stationary phases............. 133
7.8 Column care and regeneration............. 136
8 HPLC Column Tests...................... 141
8.1 Simple tests for HPLC columns............ 141
8.2 Determination of particle size............. 143
8.3 Determination of breakthrough time.......... 144
8.4 The test mixture.................... 146
8.5 Dimensionless parameters for HPLC column
characterization.................... 148
8.6 The van Deemter equation from reduced
parameters and its use in column diagnosis..... 152
8.7 van Deemter curves and other coherences....... 153
8.8 Diffusion coefficients.................. 155
9 Adsorption Chromatography: Normal-Phase
Chromatography....................... 159
9.1 What is adsorption?.................. 159
9.2 The eluotropic series.................. 162
9.3 Selectivity properties of the mobile phase....... 165
9.4 Choice and optimization of the mobile phase..... 166
9.5 Applications...................... 168
10 Reversed-Phase Chromatography.............. 173
10.1 Principle........................ 173
10.2 Mobile phases in reversed-phase chromatography . . 174
10.3 Solvent selectivity and strength............ 177
10.4 Stationary phases................... 181
10.5 Method development in reversed-phase
chromatography.................... 185
10.6 Applications...................... 188
10.7 Hydrophobie interaction chromatography....... 191
11 Chromatography with Chemically Bonded Phases..... 195
11.1 Introduction...................... 195
11.2 Properties of some stationary phases......... 195
11.3 Hydrophilic interaction chromatography....... 200
12 Ion-Exchange Chromatography............... 203
12.1 Introduction...................... 203
12.2 Principle........................ 203
12.3 Properties of ¡on exchangers.............. 204
x Contents
12.4 Influence of the mobile phase............. 207
12.5 Special possibilities of ¡on exchange......... 208
12.6 Practical hints..................... 210
12.7 Applications...................... 213
13 Ion-Pair Chromatography.................. 217
13.1 Introduction...................... 217
13.2 Ion-pair chromatography in practice.......... 218
13.3 Applications...................... 220
13.4 Appendix: UV detection using ion-pair reagents . . . 221
14 Ion Chromatography..................... 225
14.1 Principle........................ 225
14.2 Suppression techniques................ 226
14.3 Phase systems..................... 226
14.4 Applications...................... 230
15 Size-Exclusion Chromatography............... 231
15.1 Principle........................ 231
15.2 The calibration chromatogram............. 234
15.3 Molecular mass determination by means
of size-exclusion chromatography........... 238
15.4 Coupled size-exclusion columns............ 241
15.5 Phase systems..................... 243
15.6 Applications...................... 244
16 Affinity Chromatography................... 249
16.1 Principle........................ 249
16.2 Affinity chromatography as a special case of HPLC . . 251
16.3 Applications...................... 252
17 Choice of Method...................... 255
17.1 The various possibilities................ 255
17.2 Method transfer.................... 260
18 Solving the Elution Problem................. 263
18.1 The elution problem.................. 263
18.2 Solvent gradients................... 264
18.3 Column switching................... 270
18.4 Comprehensive two-dimensional HPLC........ 272
Contents xi
18.5 Optimization of an isocratic chromatogram using
four solvents...................... 273
18.6 Optimization of the other parameters......... 276
18.7 Mixed stationary phases................ 284
19 Analytical HPLC........................ 285
19.1 Qualitative analysis.................. 285
19.2 Trace analysis..................... 287
19.3 Quantitative analysis.................. 291
19.4 Recovery........................ 296
19.5 Peak-height and peak-area determination
for quantitative analysis................ 299
19.6 Integration errors.................... 303
19.7 The detection wavelength............... 304
19.8 Derivatization...................... 306
19.9 Unexpected peaks: Ghost and system peaks..... 308
20 Quality Assurance...................... 311
20.1 Is it worth the effort?.................. 311
20.2 Verification with a second method.......... 312
20.3 Method validation................... 312
20.4 Standard operating procedures............ 314
20.5 Measurement uncertainty............... 315
20.6 Qualifications, instrument test and system
suitability test..................... 317
20.7 The quest for quality.................. 318
21 Preparative HPLC....................... 321
21.1 Problem........................ 321
21.2 Preparative HPLC in practice.............. 322
21.3 Overloading effects.................. 325
21.4 Fraction collection................... 328
21.5 Recycling........................ 330
21.6 Displacement chromatography............ 331
22 Separation of Enantiomers................. 333
22.1 Introduction...................... 333
22.2 Chiral mobile phases................. 335
22.3 Chiral liquid stationary phases............ 336
22.4 Chiral solid stationary phases............. 337
22.5 Indirect separation of enantiomers.......... 345
x¡¡ Contents
23 Special Possibilities...................... 349
23.1 Micro, capillary and chip HPLC............ 349
23.2 High-speed and super-speed HPLC.......... 352
23.3 Fast separations at 1000 bar: UHPLC......... 353
23.4 HPLC with supercritical mobile phases........ 355
23.5 HPLC with superheated water............. 359
23.6 Electrochromatography................ 361
24 Appendix 1: Applied HPLC Theory.............. 363
25 Appendix 2: How to Perform the Instrument Test...... 373
25.1 Introduction...................... 373
25.2 Test sequence..................... 373
25.3 Preparations...................... 374
25.4 Pumptest........................ 377
25.5 UV detector test.................... 379
25.6 Autosampler test.................... 383
25.7 Column oven test................... 383
25.8 Equations and calculations............... 384
25.9 Documentation..................... 385
26 Appendix 3: Troubleshooting................ 387
26.1 Pressure problems................... 387
26.2 Leak in the pump system............... 389
26.3 Deviating retention times............... 389
26.4 Injection problems................... 390
26.5 Baseline problems................... 390
26.6 Peak shape problems................. 392
26.7 Problems with light-scattering detectors....... 393
26.8 Other causes...................... 394
26.9 Instrument test..................... 395
27 Appendix 4: Column Packing................. 397
Index of Separations........................ 401
Subject Index............................ 403
|
| any_adam_object | 1 |
| author | Meyer, Veronika R. 1951- |
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| ctrlnum | (OCoLC)699679746 (DE-599)BVBBV036429151 |
| discipline | Chemie / Pharmazie Chemie |
| edition | 5. ed. |
| format | Book |
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| spelling | Meyer, Veronika R. 1951- Verfasser (DE-588)108936406 aut Praxis der Hochleistungs-Flüssigchromatographie Practical high-performance liquid chromatography Veronika R. Meyer Practical high performance liquid chromatography 5. ed. Chichester [u.a.] Wiley 2010 XIII, 412 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier High performance liquid chromatography HPLC (DE-588)4072641-1 gnd rswk-swf Experiment (DE-588)4015999-1 gnd rswk-swf Flüssigkeitschromatographie (DE-588)4017622-8 gnd rswk-swf (DE-588)4123623-3 Lehrbuch gnd-content HPLC (DE-588)4072641-1 s Experiment (DE-588)4015999-1 s DE-604 Flüssigkeitschromatographie (DE-588)4017622-8 s 1\p DE-604 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020301765&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Meyer, Veronika R. 1951- Practical high-performance liquid chromatography High performance liquid chromatography HPLC (DE-588)4072641-1 gnd Experiment (DE-588)4015999-1 gnd Flüssigkeitschromatographie (DE-588)4017622-8 gnd |
| subject_GND | (DE-588)4072641-1 (DE-588)4015999-1 (DE-588)4017622-8 (DE-588)4123623-3 |
| title | Practical high-performance liquid chromatography |
| title_alt | Praxis der Hochleistungs-Flüssigchromatographie Practical high performance liquid chromatography |
| title_auth | Practical high-performance liquid chromatography |
| title_exact_search | Practical high-performance liquid chromatography |
| title_full | Practical high-performance liquid chromatography Veronika R. Meyer |
| title_fullStr | Practical high-performance liquid chromatography Veronika R. Meyer |
| title_full_unstemmed | Practical high-performance liquid chromatography Veronika R. Meyer |
| title_short | Practical high-performance liquid chromatography |
| title_sort | practical high performance liquid chromatography |
| topic | High performance liquid chromatography HPLC (DE-588)4072641-1 gnd Experiment (DE-588)4015999-1 gnd Flüssigkeitschromatographie (DE-588)4017622-8 gnd |
| topic_facet | High performance liquid chromatography HPLC Experiment Flüssigkeitschromatographie Lehrbuch |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020301765&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT meyerveronikar praxisderhochleistungsflussigchromatographie AT meyerveronikar practicalhighperformanceliquidchromatography |