Protein secretion: methods and protocols
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York, NY [u.a.]
Humana Press
2010
|
| Schriftenreihe: | Methods in molecular biology
619 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XV, 438 S. Ill., graph. Darst. |
| ISBN: | 9781603271677 |
Internformat
MARC
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| 008 | 100406s2010 ad|| |||| 00||| eng d | ||
| 020 | |a 9781603271677 |9 978-1-60327-167-7 | ||
| 035 | |a (OCoLC)636817519 | ||
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| 245 | 1 | 0 | |a Protein secretion |b methods and protocols |c ed. by Anastassios Economou |
| 264 | 1 | |a New York, NY [u.a.] |b Humana Press |c 2010 | |
| 300 | |a XV, 438 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 619 | |
| 650 | 0 | 7 | |a Proteinsekretion |0 (DE-588)4129983-8 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4143413-4 |a Aufsatzsammlung |2 gnd-content | |
| 689 | 0 | 0 | |a Proteinsekretion |0 (DE-588)4129983-8 |D s |
| 689 | 0 | |C b |5 DE-604 | |
| 700 | 1 | |a Economou, Anastassios |4 edt | |
| 830 | 0 | |a Methods in molecular biology |v 619 |w (DE-604)BV035362695 |9 619 | |
| 856 | 4 | 2 | |m Digitalisierung UB Regensburg |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018998121&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-018998121 | ||
Datensatz im Suchindex
| _version_ | 1804141181516382208 |
|---|---|
| adam_text | Contents
Preface
..........................................
v
Contributors
....................................... xi
1.
The Extraordinary Diversity of Bacterial Protein Secretion Mechanisms
..... 1
I. Barry Holland
2.
In Vitro and In Vivo Approaches to Studying the Bacterial Signal
Peptide
Processing
................................ 21
Peng Wang and Ross E. Dalbey
3.
Membrane Insertion of Small Proteins
...................... 39
Andreas
Kuhn,
Natalie
Stiegler,
and
Anne-Kathrin
Schubert
4.
Membrane Protein Insertion in
E. coli
...................... 63
Jijun Yuan, Ross E. Dalbey, and Andreas
Kuhn
5.
Study of Polytopic Membrane Protein Topological Organization
as a Function of Membrane
Lipid
Composition
.................. 79
Mikhail Bogdanov, Philip
N.
Heacock, and William Dowhan
6.
In Vivo Analysis of Protein
Translocation
to the Escherichia
coli Periplasm
.... 103
Dominique Belin
7.
Sorting of Bacterial Lipoproteins to the Outer Membrane
by the
Lol
System
.................................117
Shin-ichiro Narita and Hajime Tokuda
8.
Purification and Functional
Reconstitution
of the Bacterial Protein
Translocation
Pore, the SecYEG Complex
....................131
Ilja
Kusters,
Geert
van
den Bogaart, Janny de
Wit,
Viktor Krasnikov,
Bert
Poolman, and
Arnold Driessen
9.
Reconstitution
of the
SecY Translocon in Nanodiscs
...............145
Kush
Dalai and
Franck
Duong
10.
In Vitro
Assays to Analyze
Translocation
of the Model Secretory
Preprotein Alkaline Phosphatase
.........................157
Giorgos Gouridis, Spyridoula Karamanou, Marina Koukaki,
and Anastassios Economou
11.
Characterization of Interactions Between Proteins Using Site-Directed
Spin Labeling and Electron Paramagnetic Resonance Spectroscopy
........173
Jennine M. Crane, Angela A. Lilly, and Linda L. Randall
VII
viii Preface
12.
Analysis
of
Tat Targeting
Function and
Twin-Arginine Signal
Peptide
Activity
in Escherichia
coli
.............................191
Tracy Palmer, Ben
С.
Berks, and Frank Sargent
13.
Site-Specific Cross-Linking of In Vitro Synthesized
E. coli
Preproteins
for Investigating
Transmembrane Translocation
Pathways
............217
Sascha Panahandeh
and Matthias
Müller
14.
Tracking the Secretion of Fluorescently Labeled Type III Effectors
from Single Bacteria in Real Time
.........................241
Nandi Simpson, Laurent Audry, and Jost Enninga
15.
Comparative Analysis of Cytoplasmic Membrane Proteomes
οι
Escherichia-
coli
Using 2D Blue Native/SDS-PAGE
..............257
Susan
Schlegel, Mirjam Klepsch,
David
Wickström,
Samuel Wagner, and
Jan-Willem de Gier
16.
Using Hidden Markov Models to Discover New Protein Transport Machines
. . 271
Vladimir A. Likic, Pavel
Doležal, Nermin
Celik, Michael Dagley,
and Trevor Lithgow
17.
Bioinformatics Predictions of Localization and Targeting
.............285
Shruti Rastogi and
Burkhard Rost
18.
The
Chloroplast
Protein Import Machinery: A Review
..............307
Penelope
Strittmatter, Jürgen Soll,
and
Bettina
Bolter
19.
Measurement of the Energetics of Protein Transport Across
the
Chloroplast Thylakoid
Membrane
.......................323
Steven M. Theg
20.
In Vitro Dissection of Protein
Translocation
into the Mammalian
Endoplasmic Reticulum
..............................339
Ajay Sharma, Malaiyalam Mariappan, Suhila Appathurai,
and Ramanujan S. Hegde
21.
In Vitro
Reconstitution
of the Selection, Ubiquitination,
and Membrane Extraction of a Polytopic
ERAD
Substrate
............365
Kunio Nakatsukasa and Jeffrey L.
Brodsky
22.
Studying the ArfGAP-Dependent Conformational Changes
inSNAREs
....................................377
Fernanda Rodriguez and Anne Spang
23.
Studying Endoplasmic Reticulum Function In Vitro Using siRNA
........389
Cornelia M. Wilson and Stephen High
24.
High-Quality Immunofluorescence of Cultured Cells
...............403
Dibyendu Bhattacharyya, Adam T. Hammond,
and Benjamin S. Glick
Preface
ix
25.
Trapping
Oxidative
Folding Intermediates During
Translocation
to the
Intermembrane
Space of Mitochondria: In Vivo and In Vitro Studies
. . . 411
Dionisia P. Sideris and
Kostas Tokatlidis
26.
Native Techniques for Analysis of Mitochondrial Protein Import
.........425
F.-Nora
Vößtle,
Oliver Schmidt,
Agnieszka Chacinska,
Nikolaus Pfanner,
and Chris Meisinger
Subject Index
.......................................437
|
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| illustrated | Illustrated |
| indexdate | 2024-07-09T22:11:47Z |
| institution | BVB |
| isbn | 9781603271677 |
| language | English |
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| physical | XV, 438 S. Ill., graph. Darst. |
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| series | Methods in molecular biology |
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| spelling | Protein secretion methods and protocols ed. by Anastassios Economou New York, NY [u.a.] Humana Press 2010 XV, 438 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 619 Proteinsekretion (DE-588)4129983-8 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Proteinsekretion (DE-588)4129983-8 s b DE-604 Economou, Anastassios edt Methods in molecular biology 619 (DE-604)BV035362695 619 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018998121&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Protein secretion methods and protocols Methods in molecular biology Proteinsekretion (DE-588)4129983-8 gnd |
| subject_GND | (DE-588)4129983-8 (DE-588)4143413-4 |
| title | Protein secretion methods and protocols |
| title_auth | Protein secretion methods and protocols |
| title_exact_search | Protein secretion methods and protocols |
| title_full | Protein secretion methods and protocols ed. by Anastassios Economou |
| title_fullStr | Protein secretion methods and protocols ed. by Anastassios Economou |
| title_full_unstemmed | Protein secretion methods and protocols ed. by Anastassios Economou |
| title_short | Protein secretion |
| title_sort | protein secretion methods and protocols |
| title_sub | methods and protocols |
| topic | Proteinsekretion (DE-588)4129983-8 gnd |
| topic_facet | Proteinsekretion Aufsatzsammlung |
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