RT-PCR protocols:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, N.J.
Humana Press
2010
|
| Ausgabe: | 2. ed. |
| Schriftenreihe: | Methods in molecular biology
630 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XI, 341 S. Ill., graph. Darst. |
| ISBN: | 9781607616283 |
Internformat
MARC
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| 245 | 1 | 0 | |a RT-PCR protocols |c ed. by Nicola King |
| 250 | |a 2. ed. | ||
| 264 | 1 | |a Totowa, N.J. |b Humana Press |c 2010 | |
| 300 | |a XI, 341 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 630 | |
| 490 | 0 | |a Springer protocols | |
| 650 | 4 | |a Polymerase chain reaction |v Laboratory manuals | |
| 650 | 4 | |a Reverse Transcriptase Polymerase Chain Reaction |v Laboratory Manuals | |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-018981661 | ||
Datensatz im Suchindex
| _version_ | 1804141154332049408 |
|---|---|
| adam_text | Contents
Preface
.............................................................
v
Contributors.
............................................................ ix
Part I The RT-PCR Detective: Hunting Down the Best Method
1
Single Cell RT-PCR on Mouse Embryos: A General Approach
for Developmental Biology
.......................................... 3
Wolfgang Mann and Thomas
Haaf
2
Poly(A) cDNA Real-Time PCR for Indicator Gene Measurement in Cancer
..... 13
Melissa Oliveira-Cunha, Ajith K. Siriwardena, and Richard J. Byers
3
Transcriptome
Profiling of Host-Microbe Interactions
by Differential Display RT-PCR
...................................... 33
Leong Wai Book and Vincent
Т.К.
Chow
4
Quantitative RT-PCR Methods for Mature microRNA Expression Analysis
...... 49
Stephanie D. Fiedler, Martha Z. Carletti, and Lane K. Christenson
■ 5
Detection of Influenza A Virus Neuraminidase and PB2 Gene
Segments by One Step Reverse Transcription Polymerase Chain Reaction
....... 65
Alejandra
Castillo Alvarez, Victoria Boyd, Richard Lai,
Sandy Pineda, Cheryl Bletchly, Hans G. Heine, and Ross Barnard
6
Detection and Identification of CD46 Splicing Isoforms
by Nested RT-PCR
................................................ 83
Anita
Szalmás, József Kónya, István Sziklai, and Tamás Karost
7
Simultaneous Detection of Bluetongue Virus
RNA,
Internal
Control GAPDH mRNA, and External Control Synthetic
RNA
by Multiplex
Real-Time PCR
.................................... 97
Frank Vandenbussche,
Elise
Vandemeulebroucke, and Kris
De Clercq
8
Detection of West Nile Viral
RNA
from Field-Collected Mosquitoes
in Tropical Regions by Conventional and Real-Time RT-PCR
................ 109
Ana Silvia
González-Reiche, Marta de Lourdes Monzón-Pineda,
Barbara
W.Johnson, and
María Eugenia Morales-Betoulle
9
Detection of
Antisense
RNA
Transcripts by Strand-Specific RT-PCR
........... 125
Eric C.H. Ho, Michael E. Donaldson, and Barry J. Saville
10
RT-PCR Amplification and Cloning of Large Viral Sequences
................ 139
Xiaofeng Fan and Adrian M.
Di
Bisceglie
Part II The RT-PCR Mathematician: Assessing Gene
and
RNA
Expression
11
One-Step RT-LATE-PCR for mRNA and Viral
RNA
Detection
and Quantification
................................................ 153
Cristina
Hartshorn and Lawrence J. Wangh
vn
viii Contents
12
Changes
in Gene Expression
of Caveolin-l After Inflammatory
Pain Using Quantitative
Real-Time PCR
............................... 187
Fiza
Rashid-Doubell
13
Real-Time
Quantitative Reverse Transcriptase Polymerase Chain Reaction
...... 199
Hongxin Fan and Ryan S. Robetorye
14
The Use of Comparative Quantitative RT-PCR to Investigate
the Effect of
Cysteine
Incubation on GPxl Expression
in Freshly Isolated Cardiomyocytes
.................................... 215
Nicola King
15
The Renaissance of Competitive PCR as an Accurate Tool
for Precise Nucleic Acid Quantification
................................. 233
Lorena
Zentilin
and
Mauro Giacca
Part III The RT-PCR Master Chef: Finding the Right Ingredients
16
Skeletal Muscle
RNA
Extraction in Preparation for RT-PCR
................. 251
Janelle
P.
Mollica
17
Reverse Transcription of the Ribonucleic Acid: The First Step
in RT-PCRAssay
................................................. 261
Fadia Haddad and Kenneth M. Baldwin
18
Primer Design for RT-PCR
.......................................... 271
Kelvin Li and Anushka Brownley
19
Hot Start PCR
................................................... 301
Natasha Paul, Jonathan Shunt, and Tony
Le
20
Real-time RT-PCR for Automated Detection of
HIV-1
RNA
During Blood Donor Screening
...................................... 319
Jens Müller
Index
.................................................................. 337
|
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| discipline | Biologie |
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| id | DE-604.BV036091061 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T22:11:22Z |
| institution | BVB |
| isbn | 9781607616283 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-018981661 |
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| physical | XI, 341 S. Ill., graph. Darst. |
| publishDate | 2010 |
| publishDateSearch | 2010 |
| publishDateSort | 2010 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | RT-PCR protocols ed. by Nicola King 2. ed. Totowa, N.J. Humana Press 2010 XI, 341 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 630 Springer protocols Polymerase chain reaction Laboratory manuals Reverse Transcriptase Polymerase Chain Reaction Laboratory Manuals Methode (DE-588)4038971-6 gnd rswk-swf Reverse Transkriptase-Polymerase-Kettenreaktion (DE-588)7544647-9 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Reverse Transkriptase-Polymerase-Kettenreaktion (DE-588)7544647-9 s Methode (DE-588)4038971-6 s DE-604 King, Nicola Sonstige oth Methods in molecular biology 630 (DE-604)BV035362695 630 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018981661&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | RT-PCR protocols Methods in molecular biology Polymerase chain reaction Laboratory manuals Reverse Transcriptase Polymerase Chain Reaction Laboratory Manuals Methode (DE-588)4038971-6 gnd Reverse Transkriptase-Polymerase-Kettenreaktion (DE-588)7544647-9 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)7544647-9 (DE-588)4143413-4 |
| title | RT-PCR protocols |
| title_auth | RT-PCR protocols |
| title_exact_search | RT-PCR protocols |
| title_full | RT-PCR protocols ed. by Nicola King |
| title_fullStr | RT-PCR protocols ed. by Nicola King |
| title_full_unstemmed | RT-PCR protocols ed. by Nicola King |
| title_short | RT-PCR protocols |
| title_sort | rt pcr protocols |
| topic | Polymerase chain reaction Laboratory manuals Reverse Transcriptase Polymerase Chain Reaction Laboratory Manuals Methode (DE-588)4038971-6 gnd Reverse Transkriptase-Polymerase-Kettenreaktion (DE-588)7544647-9 gnd |
| topic_facet | Polymerase chain reaction Laboratory manuals Reverse Transcriptase Polymerase Chain Reaction Laboratory Manuals Methode Reverse Transkriptase-Polymerase-Kettenreaktion Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018981661&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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