Gene expression profile and mechanism of apoptosis inhibition in host cells infected by intracellular bacteria Chlamydia pneumoniae:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2004
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | II, 87 Bl. Ill., graph. Darst. 29 cm |
Internformat
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
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| 001 | BV036082370 | ||
| 003 | DE-604 | ||
| 007 | t| | ||
| 008 | 100317s2004 gw ad|| m||| 00||| eng d | ||
| 015 | |a 0501,H,1671 |2 dnb | ||
| 016 | 7 | |a 972654666 |2 DE-101 | |
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| 035 | |a (DE-599)BVBBV036082370 | ||
| 040 | |a DE-604 |b ger |e rakddb | ||
| 041 | 0 | |a eng | |
| 044 | |a gw |c XA-DE | ||
| 049 | |a DE-355 | ||
| 084 | |a 610 |2 sdnb | ||
| 100 | 1 | |a Limmon, Gino Valentino |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Gene expression profile and mechanism of apoptosis inhibition in host cells infected by intracellular bacteria Chlamydia pneumoniae |c von Gino Valentino Limmon |
| 264 | 1 | |c 2004 | |
| 300 | |a II, 87 Bl. |b Ill., graph. Darst. |c 29 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Jena, Univ., Diss., 2004 | ||
| 650 | 4 | |a Apoptosis | |
| 650 | 4 | |a Chlamydia pneumoniae | |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018973414&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-018973414 | |
Datensatz im Suchindex
| _version_ | 1816445538177908736 |
|---|---|
| adam_text |
CONTENTS
ABBREVIATION
.
I
1.
INTRODUCTION
.
1
1.1.
CHLAMYDIAE
PNEUMONIAE
AN
INTRACELLULAR
P
ATHOGEN
.
1
1.1.1.
TAXONOMY
.
1
1.1.2.
PATHOGENESIS
.
2
1.1.3.
DEVELOPMENTAL
CYCLE
.
3
1.1.4.
MORPHOLOGY
AND
STRUCTURE
.
4
1.1.5.
METABOHSM
.
5
1.2.
CHLAMYDIAE
AND
HOST
CELL
INTERACTION
.
6
1.2.1.
ATTACHMENT
AND
ENTRY
.
6
1.2.2.
SIGNAL
TRANSDUCTION
.
7
1.2.3.
ACTIVATION
OF
IMMUNE
RESPONSES
(INFLAMMATION)
.
8
1.2.4.
APOPTOSIS
.
9
1.3.
ASSOCIATION
BETWEEN
CHLAMYDIA
PNEUMONIAE
INFECTION
AND
ATHEROSCLEROSIS
.
11
1.4.
MICROARRAY
.
13
2.
MATERIALS
AND
METHODS
.
15
2.1.
MATERIALS
.
15
2.1.1.
CELL
LINES
.
15
2.1.1.1.
EPITHELIAL
CELLS
(HEP-2)
.
15
2.1.1.2.
FIBROBLAST
CELLS
.
15
2.1.1.3.
HUMAN
UMBILICAL
VEIN
ENDOTHELIAL
CELLS
(HUVEC).
15
2.1.2.
MEDIUM,
BUFFERS
AND
SOLUTIONS
.
15
2.1.3.
CHEMICALS
.
17
2.1.4.
ENZYMES
AND
ANTIBODIES
.
18
2.1.5.
KITS
AND
DEVICES
.
18
2.1.6.
SOFTWARE
.
19
2.2.
METHODS
.
20
2.2.1.
EXPERIMENTAL
SETUP
.
20
2.2.2.
MICROARRAY
(CHIP)
CONSTRUCTION
.
20
2.2.2.1.
PRESCREENING
(FILTER
HYBRIDIZATION)
.
20
2.2.2.2.
PROBE
PREPARATION,
SPOTTING,
AND
POST
PROCESSING
OF
ARRAY
.
21
2.2.3.
TARGET
PREPARATION
AND
HYBRIDIZATION
.
23
2.2.3.1.
CELL
CULTURE
.
23
2.2.3.2.
INFECTION
OF
CELL
LINES
WITH
CHLAMYDIA
PNEUMONIAE
.
23
2.2.4.
REVERSE
TRANSCRIPTION,
LABELLING,
HYBRIDIZATION
AND
WASHING.
24
2.2.5.
DATA
ANALYSIS
.
25
2.2.5.1.
SCANNING
AND
RAW
DATA
EXTRACTION
.
25
2.2.5.2.
DATA
NORMAHZATION,
THRESHOLDING
AND
FILTERING
.
26
2.2.6.
REAL
TIME
PCR
.
26
2.2.7.
APOPTOSIS
ASSAYS
.
28
2.2.8.
WESTERN
BLOT
EXPERIMENT
.
28
3.
RESULTS
.
30
3.1.
TECHNICAL
PART
.
30
3.1.1.
MICROARRAY
.
30
3.1.1.1.
PRESCREENING
(FILTER
HYBRIDIZATION)
.
30
3.1.1.2.
PROBE
PREPARATION
.
31
3.1.1.2.1.
AMPLIFICATION
AND
PURIFICATION
OF
PCR
PRODUCTS
.
31
3.1.1.2.2.
IMMOBILIZATION
OF
PROBES
AND
POST
PROCESSING
OF
ARRAY.
32
3.1.1.3.
TARGET
PREPARATION
.
34
3.1.1.3.1.
CELL
CULTURE
AN
INFECTION
OF
CELL
LINES
WITH
CHLAMYDIA
PNEUMONIAE
.
34
3.1.1.3.2.
TOTAL
RNA
AND
MRNA
EXTRACTION
.
35
3.1.1.3.3.
REVERSE
TRANSCRIPTION
AND
LABELLING
.
36
3.1.1.4.
HYBRIDIZATION
.
37
3.1.1.5.
DATA
ANALYSIS.
39
3.1.1.5.1.
NORMALIZATION
STRATEGY
.
39
3.1.1.5.2.
CLUSTER
ANALYSIS
.
42
3.2.
BIOLOGICAL
PART
.
45
3.2.1.
PRO
AND
ANTI
INFLAMMATORY
CYTOKINES.
48
3.2.1.1.
CHEMOKINES
AND
CHEMOKINE
RECEPTORS
.
48
3.2.1.2.
INTERLEUKINS
AND
INTERLEUKIN
RECEPTORS
.
49
3.2.1.3.
INTERFERONS
AND
INTERFERON
RECEPTORS
.
50
3.2.1.4.
TUMOUR
NECROSIS
FACTOR
AND
TNF
RECEPTORS
.
51
3.2.2.
SIGNAL
TRANSDUCTION
.
52
3.2.3.
CELL
CYCLE
.
53
3.2.4.
APOPTOSIS
.
55
3.2.5.
REAL
TIME
PCR
.
55
3.2.6.
APOPTOSIS
ASSAYS.
59
3.2.7.
WESTERN
BLOT
EXPERIMENT
.
61
4.
DISCUSSION
.
64
4.1.
GENE
EXPRESSION
PROFILE
.
64
4.2.
ACTIVATION
OF
IMMUNE
REACTION
.
65
4.3.
ACTIVATION
OF
SIGNAL
TRANSDUCTION
.
69
4.4.
CELL
CYCLE
.
70
4.5.
APOPTOSIS
.
71
REFERENCES
.
76
ZUSAMMENFASSUNG
SELBSTANDIGKEITSERKLARUNG |
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| genre_facet | Hochschulschrift |
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| illustrated | Illustrated |
| indexdate | 2024-11-22T17:44:16Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-018973414 |
| oclc_num | 253881744 |
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| physical | II, 87 Bl. Ill., graph. Darst. 29 cm |
| publishDate | 2004 |
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| publishDateSort | 2004 |
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| spelling | Limmon, Gino Valentino Verfasser aut Gene expression profile and mechanism of apoptosis inhibition in host cells infected by intracellular bacteria Chlamydia pneumoniae von Gino Valentino Limmon 2004 II, 87 Bl. Ill., graph. Darst. 29 cm txt rdacontent n rdamedia nc rdacarrier Jena, Univ., Diss., 2004 Apoptosis Chlamydia pneumoniae (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018973414&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Limmon, Gino Valentino Gene expression profile and mechanism of apoptosis inhibition in host cells infected by intracellular bacteria Chlamydia pneumoniae Apoptosis Chlamydia pneumoniae |
| subject_GND | (DE-588)4113937-9 |
| title | Gene expression profile and mechanism of apoptosis inhibition in host cells infected by intracellular bacteria Chlamydia pneumoniae |
| title_auth | Gene expression profile and mechanism of apoptosis inhibition in host cells infected by intracellular bacteria Chlamydia pneumoniae |
| title_exact_search | Gene expression profile and mechanism of apoptosis inhibition in host cells infected by intracellular bacteria Chlamydia pneumoniae |
| title_full | Gene expression profile and mechanism of apoptosis inhibition in host cells infected by intracellular bacteria Chlamydia pneumoniae von Gino Valentino Limmon |
| title_fullStr | Gene expression profile and mechanism of apoptosis inhibition in host cells infected by intracellular bacteria Chlamydia pneumoniae von Gino Valentino Limmon |
| title_full_unstemmed | Gene expression profile and mechanism of apoptosis inhibition in host cells infected by intracellular bacteria Chlamydia pneumoniae von Gino Valentino Limmon |
| title_short | Gene expression profile and mechanism of apoptosis inhibition in host cells infected by intracellular bacteria Chlamydia pneumoniae |
| title_sort | gene expression profile and mechanism of apoptosis inhibition in host cells infected by intracellular bacteria chlamydia pneumoniae |
| topic | Apoptosis Chlamydia pneumoniae |
| topic_facet | Apoptosis Chlamydia pneumoniae Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018973414&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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