Application of disaccharides pre-treatment in improving tolerances of Lactobacillus rhamnosus strains to environmental stresses or during vacuum- and spray drying processes:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Göttingen
Cuvillier
2009
|
| Ausgabe: | 1. Aufl. |
| Schlagworte: | |
| Online-Zugang: | Inhaltstext Inhaltsverzeichnis |
| Beschreibung: | Zsfassung in dt. Sprache |
| Beschreibung: | XVI, 139 S. Ill., graph. Darst. |
| ISBN: | 9783869551708 |
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IMAGE 1
TABLE OF CONTENTS
ZUSAMMENFASSUNG I
ABSTRACT III
DEDICATION V
ACKNOWLEDGEMENTS VII
TABLE OF CONTENTS IX
LIST OF FIGURES XI
LIST OF TABLES XV
LIST OF MEDIA AND REAGENTS XVI
1 INTRODUCTION AND OBJECTIVE OF WORK 1
2 LITERATURE REVIEW 8
2.1 PROBIOTIC PRODUCTS 8
2.1.1 DEFINITION OF PROBIOTICS 8
2.1.2 IMPORTANT FACTORS FOR THE DEVELOPMENT OF PROBIOTIC CULTURES 8
2.1.2.1 VIABILITY OF PROBIOTIC ORGANISMS 9
2.1.3 BENEFICIAL EFFECTS OF PROBIOTICS 10
2.1.3.1 ANTIMICROBIAL PROPERTIES 11
2.1.3.2 ANTIMUTAGENIO PROPERTIES 11
2.1.3.3 ANTICARCINOGENIC PROPERTIES 12
2.1.3.4 IMPROVEMENT IN LACTOSE METABOLISM 12
2.1.3.5 REDUCTION IN SERUM CHOLESTEROL 12
2.2 RESPONSES OF MICROORGANISMS TO ENVIRONMENTAL STRESSES 13
2.2.1 GENERAL INTRODUCTION 13
2.2.1.1 BACTERIAL CELL STRUCTURE 14
2.2.2 ENVIRONMENTAL STRESSFUL CONDITIONS 17
2.2.2.1 OSMOTIC STRESS 17
2.2.2.1.1 TREHALOSE 19
2.2.2.1.2 SUCROSE 21
2.2.2.2 HEAT STRESS 22
2.2.2.3 HIGH PRESSURE 23
2.2.3 FLOW CYTOMETRY 25
2.2.3.1 PRINCIPLES OF THE FLOW CYTOMETER 26
2.2.3.2 ASSESSMENT OF PHYSIOLOGICAL ATTRIBUTES OF MICROORGANISMS 27
2.2.3.2.1 MEMBRANE INTEGRITY 28
2.2.3.2.2 ESTERASE ACTIVITY 28
2.2.3.2.3 PUMP ACTIVITY 29
2.2.3.2.4 MEMBRANE POTENTIAL 30
2.3 DRYING OF BIOLOGICAL PRODUCTS 31
2.3.1 INTRODUCTION 31
2.3.2 PROTECTIVE MECHANISMS OF SUGARS ON BIOLOGICAL MATERIALS DURING
DRYING 33
2.3.2.1 MEMBRANES 33
2.3.2.2 PROTEINS 36
2.3.2.3 INTACT CELLS 37
2.3.3 DRYING OF PROBIOTIC BACTERIA 39
2.3.3.1 INTRODUCTION 39
2.3.3.1.1 SPRAY DRYING 39
2.3.3.1.2 VACUUM DRYING 41
3 MATERIALS AND METHODS 44
3.1 BACTERIAL STRAINS AND GROWTH CONDITIONS 44
3.2 OSMOTIC AGENTS 44
3.3 MEDIA 44
3.3.1 METHODS OF PREPARATION OF MEDIA 44
3.4 PLATE ENUMERATION METHOD 45
3.5 PHYSICAL TREATMENTS 45
3.5.1 SCREENING FOR THE SUB-LETHAL AND LETHAL CONDITIONS 45
3.5.1.1 OSMOTIC TREATMENTS 45
3.5.2 HIGH PRESSURE TREATMENTS 45
3.6 CHARACTERIZATION OF STRESS RESPONSES 46
3.6.1 RESPONSE OF LOG-PHASE CULTURES TO OSMOTIC TREATMENTS 46
3.6.2 ASSESSMENT OF OSMOTIC PRE-TREATED CELLS' RESPONSE TO LETHAL HEAT
STRESS 46
3.6.3 ASSESSMENT OF OSMOTIC PRE-TREATED CELLS' RESPONSE TO LETHAL
OSMOTIC STRESS 47 3.6.4 ASSESSMENT OF OSMOTIC PRE-TREATED CELLS'
RESPONSE TO BILE STRESS 47
IX
BIBLIOGRAFISCHE INFORMATIONEN HTTP://D-NB.INFO/999018264
DIGITALISIERT DURCH
IMAGE 2
3.6.5 ASSESSMENT OF PRESSURE PRE-TREATED CELLS' RESPONSE TO LETHAL HEAT
STRESS 47
3.6.6 ELECTRON MICROSCOPY 48
3.7 FLOWCYTOMETRY 48
3.7.1 STRESS TREATMENTS 48
3.7.2 ESTERASE ACTIVITY AND MEMBRANE INTEGRITY 48
3.7.3 CF EXTRUSION ACTIVITY AND KINETICS OF EXTRUSION 48
3.7.4 FLOWCYTOMETRIC MEASUREMENTS 49
3.7.4.1 DATA ANALYSIS 49
3.7.4.2 STATISTICAL ANALYSIS 50
3.8 DRYING EXPERIMENTS 50
3.8.1 VACUUM DRYING 50
3.8.1.1 ENUMERATION OF PROBIOTICS AFTER DRYING 50
3.8.1.2 FLOWCYTOMETRIC MEASUREMENTS OF SAMPLES DRIED IN VACUUM-OVEN 51
3.8.1.3 STORAGE TESTS 52
3.8.1.4 SALT TOLERANCE TEST 52
3.8.1.5 SCREENING OF BACTERIAL TOLETRANCES TO ACID, BILE, LYSOZYME AND
PEPSIN 52
3.8.2 SPRAY DRYING 52
3.8.2.1 PREPARATION OF SAMPLES 52
3.8.2.2 HEAT CHALLENGE EXPERIMENTS 53
3.8.2.3 DRYING PROCESS 53
3.8.2.4 DETERMINATION OF MOISTURE CONTENT IN DRIED SAMPLES 55
3.8.2.5 ENUMERATION OF PROBIOTICS AFTER SPRAY DRYING 55
3.8.2.6 STORAGE TESTS 56
3.8.2.7 SENSITIVITY TESTS 56
3.8.2.8 FLOWCYTOMETRIC MEASUREMENTS OF SPRAY-DRIED SAMPLES 56
3.8.3 RECOVERY RATES 57
4 RESULTS AND DISCUSSION 58
4.1 EFFECTS OF OSMOTIC CONDITIONS OF MRS- MEDIA ON GROWTH OF BACTERIAL
STRAINS 58
4.1.1 OSMOTIC STRENGTH OF GROWTH MEDIA 58
4.1.2 SUB- LETHAL AND LETHAL CONCENTRATIONS OF OSMOTIC AGENTS 59
4.2 RESPONSES OF EXPONENTIAL-PHASE CULTURES TO OSMOTIC STRESS INDUCED BY
TREHALOSE AND SUCROSE 60
4.2.1 IMPACT OF NON-IONIC PRETREATMENTS ON THE VIABILITY OF STRAINS
SUBJECTED TO SELECTED ENVIRONMENTAL STRESSES 62
4.3 FLUORESCENCE PROPERTIES OF CONTROL AND OSMOTIC-TREATED L. RHAMNOSUS
STRAINS AS INDICATED BY CFDA/PI STAINING 69
4.3.1 ANALYSIS OF FLOW CYTOMETRIC DATA 69
4.3.2 MEMBRANE INTEGRITY AND ESTERASE ACTIVITY OF TREHALOSE TREATED AND
CONTROL CELLS 70 4.3.3 MEMBRANE INTEGRITY AND ESTERASE ACTIVITY OF
SUCROSE TREATED AND CONTROL CELLS 72 4.4 COMPARISON OF THE VIABILITY
STATUS OF CELLS' RESPONSE TO CFDA/PI LABELLING AND STANDARD
CULTURABILITY ASSAY 75
4.4.1 OSMOTIC STRESS INDUCTION BY SUCROSE 76
4.4.2 OSMOTIC STRESS INDUCTION BY TREHALOSE 77
4.5 EXTRUSION OF INTRACELLULAR ACCUMULATED DYE IN RESPONSE TO GLUCOSE
ADDITION 77
4.5.1 EXTRUSION ACTIVITY IN L. RHAMNOSUS GG 78
4.5.2 EXTRUSION ACTIVITY IN L. RHAMNOSUS E800 79
4.5.3 KINETIC MEASUREMENTS OF CF-EXTRUSION RATES 81
4.6 SURVIVAL RATES OF PROBIOTIC CELLS AFTER DRYING IN VACUUM OVEN 83
4.6.1 CELLS IN THE EXPONENTIAL PHASE 83
4.6.2 CELLS IN THE STATIONARY PHASE 84
4.6.3 FLOW CYTOMETRIC ANALYSIS OF BACTERIA DRIED UNDER VACUUM 86
4.6.4 BACTERIAL TOLERANCES TO LYSOZYME, PEPSIN, ACID AND BILE 88
4.6.5 PROBIOTIC SURVIVAL RATES IN VACUUM-DRIED TREHALOSE DURING STORAGE
89
4.7 SPRAY DRYING OF PROBIOTIC BACTERIA 91
4.7.1 THERMAL TOLERANCE OF PROBIOTIC STRAINS 91
4.7.2 SURVIVAL RATES DURING SPRAY DRYING 92
4.7.3 TOLERANCE OF CELLS TO NACI, LYSOZYME AND SIMULATED GASTRIC JUICE
97
4.7.4 FLOW CYTOMETRIC ANALYSIS OF SPRAY-DRIED BACTERIAL CELLS 98
4.7.5 SCANNING ELECTRON MICROSCOPY OF SPRAY DRIED CELLS 101
4.7.6 SURVIVAL KINETICS OF SPRAY-DRIED PROBIOTICS AT NON-REFRIGERATED
STORAGE CONDITIONS 102 4.7.6.1 EFFECT OF MONOSODIUM GLUTAMATE (MSG) ON
SURVIVAL OF SPRAY-DRIED CELLS DURING STORAGE 104 4.7.7 STABILITY OF
SPRAY-DRIED L. RHAMNOSUS GG IN DAIRY BEVERAGE POWDER AND APPLE JUICE 106
4.8 INFLUENCE OF MILD PRESSURE PRE-TREATMENT ON THE THERMOTOLERANCE OF
L. RHAMNOSUS GG. 110 5 CONCLUSION AND RECOMMENDATION 112
REFERENCES 117
CURRICULUM VITAE 139 |
| any_adam_object | 1 |
| author | Sunny-Roberts, Elizabeth O. 1971- |
| author_GND | (DE-588)140622381 |
| author_facet | Sunny-Roberts, Elizabeth O. 1971- |
| author_role | aut |
| author_sort | Sunny-Roberts, Elizabeth O. 1971- |
| author_variant | e o s r eos eosr |
| building | Verbundindex |
| bvnumber | BV036052403 |
| classification_tum | LEB 420d LEB 123d |
| ctrlnum | (OCoLC)637053097 (DE-599)DNB999018264 |
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| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 664 - Food technology |
| dewey-raw | 664.001579 |
| dewey-search | 664.001579 |
| dewey-sort | 3664.001579 |
| dewey-tens | 660 - Chemical engineering |
| discipline | Chemie / Pharmazie Biologie Lebensmitteltechnologie |
| edition | 1. Aufl. |
| format | Thesis Book |
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| indexdate | 2024-07-20T10:29:47Z |
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| publisher | Cuvillier |
| record_format | marc |
| spelling | Sunny-Roberts, Elizabeth O. 1971- Verfasser (DE-588)140622381 aut Application of disaccharides pre-treatment in improving tolerances of Lactobacillus rhamnosus strains to environmental stresses or during vacuum- and spray drying processes vorgelegt von Elizabeth O. Sunny-Roberts 1. Aufl. Göttingen Cuvillier 2009 XVI, 139 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zsfassung in dt. Sprache Zugl.: Berlin, Techn. Univ., Diss., 2009 Stressreaktion (DE-588)4138565-2 gnd rswk-swf Zerstäubungstrocknung (DE-588)4190759-0 gnd rswk-swf Probiotikum (DE-588)4338112-1 gnd rswk-swf Trehalose (DE-588)4290438-9 gnd rswk-swf Lactobacillus (DE-588)4166370-6 gnd rswk-swf Saccharose (DE-588)4178816-3 gnd rswk-swf Osmose (DE-588)4043973-2 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Lactobacillus (DE-588)4166370-6 s Probiotikum (DE-588)4338112-1 s Stressreaktion (DE-588)4138565-2 s Osmose (DE-588)4043973-2 s Trehalose (DE-588)4290438-9 s Saccharose (DE-588)4178816-3 s Zerstäubungstrocknung (DE-588)4190759-0 s DE-604 text/html http://deposit.dnb.de/cgi-bin/dokserv?id=3399539&prov=M&dok_var=1&dok_ext=htm Inhaltstext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018944053&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Sunny-Roberts, Elizabeth O. 1971- Application of disaccharides pre-treatment in improving tolerances of Lactobacillus rhamnosus strains to environmental stresses or during vacuum- and spray drying processes Stressreaktion (DE-588)4138565-2 gnd Zerstäubungstrocknung (DE-588)4190759-0 gnd Probiotikum (DE-588)4338112-1 gnd Trehalose (DE-588)4290438-9 gnd Lactobacillus (DE-588)4166370-6 gnd Saccharose (DE-588)4178816-3 gnd Osmose (DE-588)4043973-2 gnd |
| subject_GND | (DE-588)4138565-2 (DE-588)4190759-0 (DE-588)4338112-1 (DE-588)4290438-9 (DE-588)4166370-6 (DE-588)4178816-3 (DE-588)4043973-2 (DE-588)4113937-9 |
| title | Application of disaccharides pre-treatment in improving tolerances of Lactobacillus rhamnosus strains to environmental stresses or during vacuum- and spray drying processes |
| title_auth | Application of disaccharides pre-treatment in improving tolerances of Lactobacillus rhamnosus strains to environmental stresses or during vacuum- and spray drying processes |
| title_exact_search | Application of disaccharides pre-treatment in improving tolerances of Lactobacillus rhamnosus strains to environmental stresses or during vacuum- and spray drying processes |
| title_full | Application of disaccharides pre-treatment in improving tolerances of Lactobacillus rhamnosus strains to environmental stresses or during vacuum- and spray drying processes vorgelegt von Elizabeth O. Sunny-Roberts |
| title_fullStr | Application of disaccharides pre-treatment in improving tolerances of Lactobacillus rhamnosus strains to environmental stresses or during vacuum- and spray drying processes vorgelegt von Elizabeth O. Sunny-Roberts |
| title_full_unstemmed | Application of disaccharides pre-treatment in improving tolerances of Lactobacillus rhamnosus strains to environmental stresses or during vacuum- and spray drying processes vorgelegt von Elizabeth O. Sunny-Roberts |
| title_short | Application of disaccharides pre-treatment in improving tolerances of Lactobacillus rhamnosus strains to environmental stresses or during vacuum- and spray drying processes |
| title_sort | application of disaccharides pre treatment in improving tolerances of lactobacillus rhamnosus strains to environmental stresses or during vacuum and spray drying processes |
| topic | Stressreaktion (DE-588)4138565-2 gnd Zerstäubungstrocknung (DE-588)4190759-0 gnd Probiotikum (DE-588)4338112-1 gnd Trehalose (DE-588)4290438-9 gnd Lactobacillus (DE-588)4166370-6 gnd Saccharose (DE-588)4178816-3 gnd Osmose (DE-588)4043973-2 gnd |
| topic_facet | Stressreaktion Zerstäubungstrocknung Probiotikum Trehalose Lactobacillus Saccharose Osmose Hochschulschrift |
| url | http://deposit.dnb.de/cgi-bin/dokserv?id=3399539&prov=M&dok_var=1&dok_ext=htm http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018944053&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT sunnyrobertselizabetho applicationofdisaccharidespretreatmentinimprovingtolerancesoflactobacillusrhamnosusstrainstoenvironmentalstressesorduringvacuumandspraydryingprocesses |